Labshake search
Citations for Diagenode :
151 - 200 of 582 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
-
bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
-
bioRxiv - Genomics 2024Quote: ... The DNA was quantified using Qubit and the fragment length was assessed with the Fragment Analyzer (HS large fragment kit) and then sheared to a length of 24 kb using the Megaruptor 3 (Diagenode) (45/200/30) ...
-
bioRxiv - Cancer Biology 2024Quote: For library preparation from ChIP-fragments the MicroPlex Library Preparation Kit v3 was used along with ‘24 UDI for MicroPlex v3 - Set I’ (Diagenode). 10 µl of every sample containing 50 pg of precipitated DNA were used for library preparation according to the manufacturer’s protocol for Template Preparation and Library Amplification ...
-
bioRxiv - Cancer Biology 2024Quote: Nuclei isolation and sample preparation was performed using the ATAC-Seq kit and the 24 UDI for Tagmented libraries - Set I (Diagenode) with 5×104 cells per condition after treatment with DOX or BRM014 (1 µM ...
-
bioRxiv - Molecular Biology 2022Quote: ... Chromatin was sheared in Bioruptor® Pico 15 mL tubes with 300 µL beads (Diagenode C30010017) by 25 cycles of 30 sec on and 30 sec off using a Bioruptor® (Diagenode B01060010) ...
-
bioRxiv - Cancer Biology 2023Quote: ... for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Finally ...
-
bioRxiv - Cell Biology 2023Quote: ... for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Finally ...
-
bioRxiv - Microbiology 2024Quote: ... Further they were sonicated (15 min, 30 s on, 30 s off, high settings; Bioruptor, Diagenode). The protein lysates were normalized to 1 mg/ml for their concentration and subjected to affinity precipitation using 15 μl Strep-II tagged beads (IBA Lifesciences GmbH ...
-
bioRxiv - Genetics 2023Quote: ... Briefly, DNA from activated B cells (day 4 with IL-4, anti-CD40) was sonicated to ∼1000 bp (Diagenode Bioruptor) before amplification via an Sµ-specific biotinylated primer (5’/5BiosG/CAGACCTGGGAATGTATGGT-3’) ...
-
bioRxiv - Cell Biology 2024Quote: ... 150 mM NaCl and FPIC) for sonication for 4 min (4 x (30 s ON, 30 s OFF)) (Bioruptor, Diagenode), yielding genomic DNA fragments with a bulk size of 150–500 bp ...
-
bioRxiv - Microbiology 2020Quote: ... high power at 4°C (Bioruptor Plus, Diagenode)] ...
-
bioRxiv - Genomics 2022Quote: ... H3K36me3 (Diagenode, C1541092-10), TFAP2A (Abcam ...
-
bioRxiv - Biochemistry 2023Quote: ... After glass-bead beating, chromatin was sonicated for 24 cycles (high output, 30 s-on, 30 s-off) using the Bioruptor Plus (Diagenode, Belgium) to produce fragments of ∼ 250 bp ...
-
bioRxiv - Molecular Biology 2022Quote: ... 30 sec off) for 15 minutes to shear chromatin to less than 500 bp with Bioruptor (Diagenode). After pelleting the insoluble fraction ...
-
bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
-
bioRxiv - Genomics 2020Quote: ... the DNA was sheared to between 15 kb and 23 kb using the Megaruptor® 3 (Diagenode). HiFi sequencing libraries were prepared28 using SMRTbell™ Express Template Prep Kit 2.0 and followed by immediate treatment with the Enzyme Clean Up Kit (PN ...
-
bioRxiv - Genomics 2022Quote: A total of 15 μg of extracted genomic DNA was sheared using a Megaruptor (Diagenode, Denville, NJ). The large DNA fragment was recycled by BluePippin (Sage Science ...
-
bioRxiv - Genomics 2022Quote: ... DNA was sheared for 26 cycles of shearing (15s on, 30s chilling) using a Bioruptor sonicator (Diagenode), then size selected using SPRI beads (Beckman ...
-
bioRxiv - Neuroscience 2024Quote: ... and sonicated with 30 cycles of 15 s ON-OFF using a BioRuptor (Diagenode, Denville, NJ-USA). The EV lysate was reduced with 10 mmol/L dithiothreitol at 56 °C for 30 minutes ...
-
bioRxiv - Genomics 2024Quote: ... HMW gDNA was sheared to 15-18 Kb using Diagenode’s Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1x of SMRTbell cleanup beads for the repair and a-tailing incubation at 37°C for 30 minutes and 65°C for 5 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... polyA-selected mRNA was adjusted to 15 ng/µL in 100ul and fragmented using Bioruptor ultrasonicator (Diagenode) with 30s on/off for 30 cycles ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
-
bioRxiv - Immunology 2024Quote: ... Samples were sonicated for 15 cycles of 30 s on/ 30 s off with a BioRuptor (Diagenode). To remove trace DNA contamination 1 μl benzonase was added to each sample followed by incubation at 37°C for 15 minutes ...
-
bioRxiv - Genomics 2023Quote: ... DNA was sheared to 20 kb using the megaruptor system (Diagenode). ONT (Oxford nanopore technologies ...
-
bioRxiv - Immunology 2021Quote: ... in a 4°C-cooled water bath sonicator (Diagenode Bioruptor ...
-
bioRxiv - Physiology 2020Quote: ... and sonicated at 4 degrees using Bioruptor sonicator (Diagenode) for 20 cycles on high setting ...
-
bioRxiv - Genomics 2021Quote: ... Per ChIP 4 µl RARα antibody (serum, Diagenode C15310155) or 2 µg IgG control (Diagenode C15410296 ...
-
bioRxiv - Genomics 2022Quote: ... by boiling at 95°C for 5 minutes followed by 5 sonication cycles of 30 s ON / 30 s OFF on the Bioruptor Pico sonicator (Diagenode) at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.1 % SDS in PBS) and sonicated 5 times for 5 min (30s ON/ 30s OFF) at high mode in a Bioruptor Pico (Diagenode). IP were performed overnight at 4°C using 1.5 mg of pre-coated epoxy Epoxy Dynabeads (M-270 ...
-
bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... Shearing took place in Bioruptor® Pico 15 ml tubes loaded with 300 μL of beads (Diagenode C30010017). Sheared chromatin was separated from insoluble material by centrifugation at 20,000 rpm for 20 min at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... Lysates were then sonicated for 15 cycles (30 s ON/30 s OFF) using the Bioruptor Pico (Diagenode). The resulting soluble cell lysates were diluted with dilution buffer (16.7 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2024Quote: ... for 15 cycles of 30 seconds on and 30 seconds off in 1.5 mL Diagenode tubes (Diagenode; #C30010016). Subsequently ...
-
bioRxiv - Developmental Biology 2024Quote: ... and subjected to ultrasonic treatment using Picoruptor (15 cycles of 30 s ON and 30 s OFF) (Diagenode). The cell lysates were then diluted with RIPA buffer for immunoprecipitation (50 mM Tris-HCl at pH 8.0 ...
-
bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lysed cells were rotated for 20 minutes and sonicated in Bioruptor (Diagenode). Cell lysates were cleared by centrifugation ...
-
bioRxiv - Genomics 2020Quote: Genomic DNA was sheared to 20 kb fragments using Megaruptor 2 (Diagenode) and size selected using the BluePippin system (Sage Science ...
-
bioRxiv - Genomics 2020Quote: ... followed by 20 min wash cycle using the provided wash buffers (DIAGENODE Immunoprecipitation Buffers ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were sonicated for 20 min in a pre-cooled Bioruptor (Diagenode) and centrifuged at 13,000 x g for 10 min to remove insoluble particles ...
-
bioRxiv - Physiology 2022Quote: ... followed by sonication (Bioruptor, 20 cycles, 30 seconds on/off, Diagenode, Belgium) and heating to 95°C for 10 minutes ...
-
bioRxiv - Developmental Biology 2022Quote: ... Total protein was extracted by incubating the islets with Laemmli buffer at 95°C for 5 mins and homogenized for another 5 mins in a sonication device (Diagenode, B01060001) at 4°C ...
-
bioRxiv - Systems Biology 2020Quote: ... Pellets were sonicated at 4°C using a Bioruptor (Diagenode) to shear the chromatin to a mean length of 300 bp by repeated cycles (16 cycles of 30 s ON and 30 s OFF) ...
-
bioRxiv - Immunology 2020Quote: ... 30 s OFF at 4°C using a Bioruptor (Diagenode) with intermittent quick vortex and centrifugation using polystyrene tubes ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were sonicated at 4°C in Bioraptor Pico (Diagenode) for 10 cycles ...
-
bioRxiv - Molecular Biology 2021Quote: ... Chromatin was fragmented by sonication (30 cycles of 15 s ‘on’ and 30 s ‘off’) with a Bioruptor (Diagenode) to 200 – 1000 bp ...
-
bioRxiv - Genetics 2024Quote: ... Cells were lysed by sonication for 15 cycles (high, 30 seconds on, 30 seconds off) using Bioruptor Plus (Diagenode). After a 15-minute incubation on ice and centrifugation (21,000 ✕ g ...
-
bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
-
bioRxiv - Cell Biology 2022Quote: ... sonicated 5 x 30 sec in a Bioruptor (Diagenode) at 4 °C ...