Labshake search
Citations for Diagenode :
451 - 500 of 582 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Spirolactone, an unprecedented antifungal β-lactone spiroketal macrolide from Streptomyces iranensisbioRxiv - Biochemistry 2024Quote: ... sonicating on high for 5 x 60 sec on/30sec off using the bioruptor pico sonication water bath (Diagenode) and finally disrupting twice with the tissuelyser (QIAGEN ...
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bioRxiv - Cell Biology 2024Quote: ... After 2 × 5 cycles of sonication (30 s “ON”, 30 s “OFF”, High intensity) with a Bioruptor UCD200 (Diagenode), the chromatin solution was diluted 10-fold to a final volume of 3 mL with ChIP dilution buffer before IP ...
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bioRxiv - Microbiology 2023Quote: ... Two µg of high molecular weight genomic DNA (final volume of 100 µL at 20 ng/µL) were sheared using the Megaruptor3 (Diagenode inc, Denville, New Jersey, USA) at speed The DNA Damage repair ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... chromatin was sheared using a Bioruptor Pico Sonicator (5 cycles: 30 seconds ON - 30 seconds OFF, fragments 100 - 300 bp, Diagenode) and 10 % of sheared chromatin was kept as input control whilst 90 % was immunoprecipitated overnight using antibodies against H3K27ac ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Cell Biology 2020Quote: ... assisted by sonication (5 cycles of 30 s ON, 30 s OFF at high power, Bioruptor Plus ultrasonic disintegrator, Diagenode). Protein concentration was measured by the BCA assay.
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Pathology 2021Quote: ... and fragmented by 5 cycles of sonication on a Bioruptor (per cycle: power = high, interval = 30s on/30s off, 5min; Diagenode) to facilitate efficient DNA clustering onto the Illumina sequencing platform ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 ml of thawed OFs were sonicated with a closed-type sonication system (UCD-200T, Biorupter, Diagenode Inc., NJ, USA) and were sequentially centrifuged at 300 g for 10 min (model 5500 with tube 430829 ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to the supernatant and sonicated for 30 s ON/OFF for 5 min at high power in Bioruptor (Diagenode). After centrifugation at 16,000 × g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Neuroscience 2024Quote: Cortex nuclei were purified as above and collected by centrifugation (500 g; 5 min) for immediate use in ATAC Library preparation (Tagmentase–loaded; Diagenode). The nuclei pellets were suspended in 25 μL 2xTagmentation Buffer (Diagenode ...
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bioRxiv - Molecular Biology 2024Quote: ... and then sonicated with series of 30 s ON / 30 s OFF for 5 min on a Bioruptor device (Diagenode) and centrifuged at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Genomics 2020Quote: ... followed by sonication at high power for 10 cycles of 30s ON/OFF (Bioruptor Plus; Diagenode). Lysates were clarified by centrifugation at 10,000g for 15min ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed for 10 minutes at RT in 11% formaldehyde solution in fixation buffer (Diagenode), diluted 1:10 in RPMI with 10% FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... split into two Eppendorf tubes and sonicated for 10 minutes in a Bioruptor® Pico (Diagenode) under standard conditions (30s on ...
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bioRxiv - Physiology 2021Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of antibody (H3K4me3 (Diagenode C15410003), H3K4me1 (abcam ab8895 and H3K27ac (Diagenode C15410196) ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were sonicated (30 s/30 s, 10 cycles, high performance) by Bioruptor (Diagenode; B01020001), followed by centrifugation at 21000xg for 20 min at 20°C ...
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bioRxiv - Plant Biology 2022Quote: ... 50 μM MG132) were added and the samples were sonicated for 10 min on Bioruptor (Diagenode) at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 ng sheared to an average fragment size of ∼300 bp (Diagenode Bioruptor Pico; B01060010) for enzymatic conversion / library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were then sonicated with 3 × 10 cycles (30 s on and off) (Bioruptor, Diagenode) to generate chromatin fragments of ∼500 bp in length ...
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bioRxiv - Neuroscience 2023Quote: ... then sonicated for 10 cycles of 30 s each on/off in a Bioruptor plus (Diagenode) in presence of 50 mg Protein Extraction beads (C20000021 ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016) to generate 100–400 bp DNA fragments ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated on ice for 10 minutes and sonicated using Bioruptor Pico sonicator (Diagenode) for 23 cycles (30s ON/30s OFF) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were sonicated (10 cycles, 30 sec high-power pulse) using a Bioruptor Plus (Diagenode) and centrifuged (10,000 x g ...
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bioRxiv - Genomics 2024Quote: ... USA) with 10 μg DNA sheared using the Megaruptor 2 system (Diagenode, Liège Science Park, Belgium) to obtain an average fragment size of 15-20 kb ...
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bioRxiv - Cell Biology 2024Quote: ... sonicated at high performance for 10 cycles (30 s on/off) using Bioruptor Plus Ultrasonicator (Diagenode), and spun down for 20 minutes at room temperature at 20000 g ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was sonicated to obtain fragments of an average size of 300-400bp using a Bioruptor Pico (Diagenode; 8 cycles; 20s on and 60s off). 3μg of sonicated DNA was used for library preparation ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% PIC) and sheared to 100-600bp fragments (average 250bp) at 4°C in a Bioruptor® Pico sonication device (Diagenode: Denville, NJ) for 5 cycles (30 sec on ...
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bioRxiv - Cancer Biology 2021Quote: ... sodium deoxycholate and 0.5% (vol/vol) N-lauroylsarcosine) and the chromatin was sheared to 300 – 800 base pair (bp) fragments by Bioruptor (Diagenode, Belgium) 12 cycles of 30 second “ON” and 30 second “OFF” intervals ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF, pH 8.5) and sonicated for 5 min (30 sec on, 1 min off) with Bioruptor UCD-200TM (Diagenode, Liège, Belgium). The resulting cell lysates were centrifuged ...
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bioRxiv - Plant Biology 2024Quote: ... 65µg of genomic DNA was split into 5 aliquots and manually sheared with the Megaruptor 3 instrument (Diagenode, P/N B06010003), according to the Megaruptor 3 operations manual ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear extracts were collected and sonicated for 10 minutes to obtain 300bp chromatin fragments (Diagenode Bioruptor Pico). Equal volumes of sheared chromatin were immunoprecipitated with rabbit AR-V7 antibody (RevMab 31-1109-00) ...
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bioRxiv - Neuroscience 2021Quote: ... and chromatin was sheared using a Bioruptor Pico sonicator (10 sonication cycles 30’’ ON/ 30’’ OFF, Diagenode). Sheared chromatin was incubated under constant rotation at 4°C O/N with Protein A-coated magnetic beads ...
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bioRxiv - Physiology 2022Quote: ... Chromatin from approximately 10 million cells was sheared using a sonication device (Bioruptor Pico, #2013-2019, Diagenode) and optimized to produce ~400bp fragments ...
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bioRxiv - Genomics 2021Quote: ... Cells were then sonicated for 10 cycles of 30 seconds on/off on a Bioruptor Pico (Diagenode) at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003] ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin from 10 million cells was sonicated to between 200-600 bp using a Bioruptor Plus (Diagenode). 100µL of Dynabeads Protein G (Invitrogen ...