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Citations for Diagenode :
301 - 343 of 343 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: Approximately 10 µg of genomic DNA was sheared to 30-50 kb using the Megaruptor 3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends ...
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bioRxiv - Genomics 2024Quote: ... DNA was sheared to 10-20 kb with a Megaruptor 3 sonicator (Diagenode, Denville, New Jersey, USA) and treated with SMRTbell cleanup beads (PacBio ...
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bioRxiv - Cancer Biology 2022Quote: ... Pellets were resuspended in 1.8mL of lysis buffer 3 (10mM Tris-HCl pH 7.5, 100mM NaCl, 1mM EDTA) and split in 6x 1.5mL sonication tubes (Diagenode). Upon 8 cycles of sonication with Pico Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2021Quote: ... and sonicated (3 cycles of the 30 sec pulse with 30 sec interval on ice) using Bioruptor (Diagenode). The mixture was then boiled at 95 °C for 5 min to denature the proteins ...
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bioRxiv - Genomics 2023Quote: 10 ug of C5 genomic DNA was sheared to 30 kb - 50 kb using the Megaruptor 3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Genomics 2023Quote: ... and 10 µg of DNA were sheared to appropriate size range (15-20 kb) using Megaruptor 3 (Diagenode). The sheared DNA was concentrated by bead purification using PB Beads (PacBio) ...
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bioRxiv - Plant Biology 2024Quote: ... Chromatin sonicated 3 times for 5 min (30 sec on/off intervals) in a Bioruptor® Plus (Diagenode) at the low setting to generate DNA fragments in the range of 200-800 bp ...
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bioRxiv - Genomics 2024Quote: ... HMW DNA was sheared to an appropriate size range (∼10-30 kb) using a Megaruptor 3 (Diagenode, USA). The sequencing library was constructed using the SMRTbell Express Template Prep Kit 2.0 following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... chromatin was sheared to 100-500bp fragments using the Bioruptor Pico sonicator for 3 cycles of 30 sec on/off in 0.5ml tubes (Diagenode). Quantitation of chromatin was performed using a qubit fluorimeter where at least 10 ng of chromatin were used per experiment ...
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bioRxiv - Plant Biology 2022Quote: ... the protocol involves fragmenting the HMW DNA to a mean fragment length of 20 kb using Megaruptor 3 (Diagenode), followed by DNA damage repair ...
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Neuroscience 2024Quote: ... the extracted DNA was sheared to a target size of 18-20 kb using the Megaruptor 3 (Diagenode, B060100003). After confirmation of correct sizing ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... by 30-min incubation on ice followed by sonicating for 3 min at medium power using a Bioruptor (Diagenode). Protein concentration was measured by Bradford assay (Bio-Rad ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... lysates were first sonicated for 3 cycles of 1 min pulse at 30 sec intervals in a Bioruptor (Diagenode) and then centrifuged at 4°C for 5 min at 1,000 g ...
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bioRxiv - Genomics 2024Quote: Long-read sequencing was facilitated by fragmenting genomic DNA using the Megaruptor 3 shearing kit (Diagenode SA, Seraing, Belgium). Fragments less than 5 kb were selectively removed using the AMPure PB beads size selection kit (Pacbio ...
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bioRxiv - Genomics 2024Quote: ... and one of two U2OS libraries were prepared by first treating 3 μg of DNA with the Short Fragment Eliminator Kit (ONT) according to the manufacturer’s protocol before shearing with the DNA Fluid+ Kit on a Megaruptor 3 (Diagenode) to a mean size of approximately 50k bp ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 30 min at 4°C with rocking and then sonicated (3 x 30 sec interval, high setting in a Bioruptor water bath sonicator, Diagenode). Lysates were cleared by centrifugation at 13,000 g for 15 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: 15 μg of DNA was cleaned with a 1X AMPure PB beads (Pacific Biosciences) cleanup and sheared to a target size of 14 kilobases (kb) using the Megaruptor 3 instrument (Diagenode) with the following settings ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Microbiology 2024Quote: ... The cells were incubated for 20 min on ice and lysed by sonication (3 cycles, 30 sec high-power pulse) using a Bioruptor Plus (Diagenode). After centrifugation (10,000 x g ...
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bioRxiv - Genomics 2024Quote: ... The DNA was quantified using Qubit and the fragment length was assessed with the Fragment Analyzer (HS large fragment kit) and then sheared to a length of 24 kb using the Megaruptor 3 (Diagenode) (45/200/30) ...
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bioRxiv - Genomics 2024Quote: ... 6.4 ug of HG008-T and 6.1 ug of HG008-N-P were sheared separately on a Megaruptor 3 (Diagenode, B06010003) to a target size of 15 to 20 kb ...
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bioRxiv - Genomics 2024Quote: A starting amount of 4-5 ug HMW gDNA was sheared to a target size of 20-30 kbp using a Megaruptor 3 instrument (Diagenode). The sheared DNA underwent size selection using a Pippin HT instrument (Sage Science ...
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bioRxiv - Neuroscience 2024Quote: ... were subjected to 3 cycles of sonication (30sec ON/30sec OFF for 10 min, at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to the sonicated lysate to a final concentration of 1% ...
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bioRxiv - Neuroscience 2024Quote: ... were subsequently subjected to 3 cycles of sonication (30s ON/30s OFF for 10 minutes at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to a final concentration of 1% ...
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bioRxiv - Genetics 2024Quote: ... We sheared the high molecular weight (HMW) DNA to achieve consistent and narrow size ranges to 20kb using the Megaruptor 3 Shearing kit (E07010003, Diagenode). Megaruptor 3 was performed using customized settings ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Molecular Biology 2024Quote: ... An input of 50 ng of genomic DNA was sheared to 6 kb - 10 kb using the Megaruptor® 3 (Diagenode) or g-TUBE (Covaris) ...
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bioRxiv - Plant Biology 2024Quote: ... 65µg of genomic DNA was split into 5 aliquots and manually sheared with the Megaruptor 3 instrument (Diagenode, P/N B06010003), according to the Megaruptor 3 operations manual ...
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bioRxiv - Developmental Biology 2021Quote: ... and subsequently sonicated for 3 cycles (30 seconds ON, 30 seconds OFF) on low settings using a Bioruptor® Plus (Diagenode, #B01020001). Samples were then centrifuged for 10 min at 10.000 g and supernatant was collected ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mM TEAB and 1 x Halt protease inhibitor cocktail) and sonication (3 × 5 min cycles of 30s on/off; Diagenode Bioruptor sonication device). Extracted proteins protein were digested to peptides with in-solution digestion performed at the Turku Proteomics Facility ...
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bioRxiv - Plant Biology 2023Quote: ChIP assays were performed on leaves and axillary buds enriched samples of 3 weeks old plants using anti-H3K27me3 (Diagenode pAb-195-050) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10.0 μg of high molecular weight purified genomic DNA (final volume of 120 μl) was sheared using the Diagenode Megaruptor 3 instrument (Diagenode Inc., Denville, NJ, USA) and a two-cycle shear method at speed 33 followed by speed 34 ...