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Citations for Diagenode :
201 - 250 of 343 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Following normalization of protein concentrations of cleared lysates ...
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bioRxiv - Genomics 2021Quote: ... pH 7.4) aided by homogenization by passing the cells through various gouge needles and sonication using the Bioruptor® sonication system (Diagenode) for 15 cycles of 30 s ON / 30 s OFF at high setting ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... and 30 s off in a sonicator (Diagenode, New Jersey, USA) and centrifuged at 14000 rpm for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 times for 30 s (maximum power, Bioruptor®plus, Diagenode). The lysate was clarified by ultracentrifugation (41 000 xG ...
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bioRxiv - Immunology 2022Quote: ... and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus). After sonication ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Genomics 2024Quote: ... at 4°C for 13 cycles with 30 sec on and 30 sec rest in 15 ml Bioruptor Tubes (Diagenode Cat #C30010017) with 650 mg of beads (Diagenode Cat #C03070001) ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated for 15 min (cycles of 30 sec ON and 30 sec OFF, 4°C, low frequency) in a Bioruptor Pico (Diagenode, Seraing, Belgium). Samples were subsequently incubated for 15 min at 70°C ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% PIC) and sheared to 100-600bp fragments (average 250bp) at 4°C in a Bioruptor® Pico sonication device (Diagenode: Denville, NJ) for 5 cycles (30 sec on ...
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bioRxiv - Cell Biology 2024Quote: ... followed by six 30 s cycles of sonication (Bioruptor Pico, Diagenode, USA). Protein concentration was measured using the BCA assay (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by six cycles of 30 s of sonication (Bioruptor Pico, Diagenode, USA). Proteins were reduced with 5 mM dithiothreitol and alkylated with 10 mM iodoacetamide in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... before 5 cycles of 20 s on/off sonication using a Bioruptor (Diagenode). Samples were centrifuged for 15 min at 14,000 g to pellet insoluble debris and lipids ...
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bioRxiv - Microbiology 2020Quote: ... followed by six cycles of 30 s of sonication (Bioruptor Pico, Diagenode, USA). Protein concentration was measured using the BCA assay (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... followed by six cycles of 30 s of sonication (Bioruptor Pico, Diagenode, USA). Protein concentration was measured using the BCA assay (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... and DNA sheared by sonication (45 cycles; 30 s on/off; Bioruptor Pico, Diagenode) in aliquots (0.2 ml) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were sonicated using a Bioruptor Plus from Diagenode (15 cycles of 30 s). The protein concentration was measured using a tryptophan assay ...
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bioRxiv - Cell Biology 2023Quote: ... 30 s off using a Bioruptor Plus Sonication System (Diagenode, Denville, NJ, United States). Protein was quantified by Bradford54 and samples were mineralized in trace metal grade nitric acid HNO3 and boiled at 100 °C.
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bioRxiv - Molecular Biology 2024Quote: ... Brains were subjected to 2 x 30 s of sonication with a Bioruptor (Diagenode), 20 min of incubation on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated for 30 minutes at 4°C (30 seconds ON, 30 seconds OFF, output setting high) using a water bath sonicator (Diagenode Bioruptor® Plus; Cat. No. B01020001), transferred to new 1.5 ml tubes ...
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bioRxiv - Cell Biology 2023Quote: ... nuclei were sonicated in ice water for 5×30 s (power: middle, Bioruptor Plus, Diagenode). Samples were centrifuged for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... crushed with a Dounce homogenizer and sonicated ten times for 30 s (maximum power, Bioruptorplus, Diagenode). After centrifugation at 10,000g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... then sonicated for 10 cycles of 30 s each on/off in a Bioruptor plus (Diagenode) in presence of 50 mg Protein Extraction beads (C20000021 ...
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bioRxiv - Cell Biology 2024Quote: ... sonicated at high performance for 10 cycles (30 s on/off) using Bioruptor Plus Ultrasonicator (Diagenode), and spun down for 20 minutes at room temperature at 20000 g ...
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bioRxiv - Plant Biology 2021Quote: ... Nucleic acids were fragmented with a Diagenode Bioruptor® Sonicator (Diagenode, Belgium). For immunoprecipitation ...
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bioRxiv - Physiology 2024Quote: ... Tissue homogenates were further processed to remove the nucleic acid traces (Diagenode SA ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
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bioRxiv - Molecular Biology 2023Quote: ... and chromatin was sheared by sonication using a Bioruptor (Diagenode, 9 cycles of 30 s on/off). Extracts were incubated for 2 h at room temperature with anti-V5 agarose beads (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated with 30 cycles of 15 s ON-OFF using a BioRuptor (Diagenode, Denville, NJ-USA). The EV lysate was reduced with 10 mmol/L dithiothreitol at 56 °C for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Then the lysate was sonicated for 22 min (30 s on/30s off) with Bioruptor Plus (Diagenode). After centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of antibody per IP (Cas9 – Diagenode C15310258 ...
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bioRxiv - Genomics 2021Quote: ... Labs 1 and 3 used Antibody 1 (Diagenode, Denville ...
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bioRxiv - Genomics 2024Quote: ... DNA was then sheared with Megaruptor 3 (Diagenode) (45/200/30 ...
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bioRxiv - Genomics 2024Quote: ... and sheared with Megaruptor 3 (Diagenode, Liège, BELGIUM) at an average size of 20 kb ...
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bioRxiv - Molecular Biology 2021Quote: ... crushed with a Dounce homogenizer and sonicated ten times for 30 s (maximum power, Bioruptor®plus, Diagenode). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... and sonicated for 7.5 min (1.5 mL tubes, high power, 30 s on/off cycle, Bioruptor plus, Diagenode). Lysates (20–30 μL ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were sheared by 10 cycles of sonication (30s on and 30 s off) in a Bioruptor (Diagenode) followed by centrifugation at 12K rpm for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... in cycles of 30 s on-off at mild intensity using a Bioruptor UCD-200 (Diagenode, Denville, NJ). Protein concentrations were quantified using the Bradford method (90 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were sonicated at high setting with 30 s on/off cycles using a Bioruptor UCD-200 (Diagenode). Lysates were cleared by centrifugation at 14000×g ...