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Citations for Diagenode :
101 - 150 of 343 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Physiology 2023Quote: ... 500µl volume) in a water bath sonicator set at 4°C (Bioruptor 300; Diagenode, Denville, NJ). After centrifuging at 10000g for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated 10 minutes at 4°C and then sheared using a Bioruptor (Diagenode, Belgium) for 4 times at 5 cycles and followed by 2 cycles at 30″ ON/OFF high power ...
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bioRxiv - Neuroscience 2024Quote: ... Thawed samples were sonicated (2 cycles [10 s], 30 s between cycles, high setting; Bioruptor Next Gen, Diagenode) and heated (+90 °C for 7 min) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were then sonicated for 15 cycles (30 s ON/30 s OFF) using the Bioruptor Pico (Diagenode). The resulting soluble cell lysates were diluted with dilution buffer (16.7 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2024Quote: ... and subjected to ultrasonic treatment using Picoruptor (15 cycles of 30 s ON and 30 s OFF) (Diagenode). The cell lysates were then diluted with RIPA buffer for immunoprecipitation (50 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin was fragmented by sonication (30 cycles of 15 s ‘on’ and 30 s ‘off’) with a Bioruptor (Diagenode) to 200 – 1000 bp ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... By ultrasonically treating the pellet three times for 30 s with intervals of 30 s with a Bioruptor (Diagenode), the proteins precipitated with acetone for 3 h at −30 °C were dispersed in 50 mM triethylammonium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated for 7.5 min (30 s on, 30 s off cycles) using a Bioruptor waterbath sonicator (Diagenode), then cleared by centrifugation at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... cells were lysed by sonication with 15 cycles (15 s on/10 s off) using the Bioruptor Plus (Diagenode) at 4C ...
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bioRxiv - Cell Biology 2024Quote: ... After 2 × 5 cycles of sonication (30 s “ON”, 30 s “OFF”, High intensity) with a Bioruptor UCD200 (Diagenode), the chromatin solution was diluted 10-fold to a final volume of 3 mL with ChIP dilution buffer before IP ...
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bioRxiv - Molecular Biology 2020Quote: ... and RISC-Poor small sequencing libraries were constructed with CATS RNA-seq kit (Diagenode C05010041 Lot#4). Library primer dimers were removed by AMPureXP beads (Beckman) ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode). Protein concentration was determined using the Micro BCA™ protein assay kit according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Genetics 2021Quote: ... incubated on ice for 10 min and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles with 30 s ON and 30 s OFF ...
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bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were sonicated for 4 min (20 seconds on, 40 seconds off) using a Bioruptor Pico (Diagenode). After sonication samples were cleared with 1% triton X-100 and pelleted (16000 x g for 5 min at 4 °C) ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Plant Biology 2020Quote: ... Nuclei were disrupted by sonication 10 times for 30 s each and 30 s of cooling in a Bioruptor (Diagenode). IPs were performed using α-GFP (Ab6556 ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was kept on ice and the pellet was resuspended in 200 µL OLB with 500 mM NaCl and sonicated for 10 min at high setting with 30 s / 30 s duty cycle on a Bioruptor sonicator (Diagenode) and centrifuged for 5 min at 20,000 × g at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was sheared by sonication for 10 min at high settings (30 s on, 30 s off) using a Bioruptor Pico sonicator (Diagenode) to reduce sample viscosity ...
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bioRxiv - Cell Biology 2020Quote: ... assisted by sonication (5 cycles of 30 s ON, 30 s OFF at high power, Bioruptor Plus ultrasonic disintegrator, Diagenode). Protein concentration was measured by the BCA assay.
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Genomics 2021Quote: ... Pellet was then resuspended in lysis buffer for 5 min on ice and sonicated as follows: 16 cycles of 30 s on / 30 s off at max power (Bioruptor Diagenode), and centrifuged at 14,000 rpm at 4 °C for 10 min ...
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bioRxiv - Genomics 2021Quote: ... 0.3% NP-40, 10% glycerol, 1XPIC) and sonicated for 10 cycles (30 s on, 30 s off) in a Bioruptor (Diagenode). SDS loading dye was added and the samples were boiled before immunoblotting.
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bioRxiv - Molecular Biology 2021Quote: ... by sonication for four cycles of 30 s pulses with low power and 60 s cooling between pulses using a Bioruptor UCD-200 (Diagenode). Following centrifugation (16,100g for 10 min at 4°C) ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were sonicated five times (30-s pulses with 30-s break interval) using the Bioruptor water bath sonicator (Diagenode). The chromatin extracts were then centrifuged at 9,400 g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... by incubation for 5 min at 99°C followed by sonication (alternating 30 s on- and 30 s off-cycles at highest intensity output (Bioruptor, Diagenode)) ...
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bioRxiv - Genomics 2022Quote: ... by boiling at 95°C for 5 minutes followed by 5 sonication cycles of 30 s ON / 30 s OFF on the Bioruptor Pico sonicator (Diagenode) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and incubation on ice for 30 min followed by sonication for 45 min (30 s on, 30 s off) in a Bioruptor Pico (Diagenode) at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and sonicated for 10 min (medium intensity, 30 s on/30 s off) in a Bioruptor UCD-200 system (Diagenode), and centrifuged at 21,000 x g for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... the resuspended solution was subjected to sonication for 22 cycles (30 s on/30 s off per cycle) with Bioruptor Plus (Diagenode). The lysate was centrifuged with 8,000g at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: 10 μg of genomic DNA were sonicated during 40 cycles (30 s ON/60 s OFF, low intensity) into 200-400 bp fragments using a Bioruptor Plus sonicator (Diagenode). Sonication efficiency was verified by electrophoresis in a 1.5% agarose gel ...
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bioRxiv - Molecular Biology 2024Quote: ... and then sonicated with series of 30 s ON / 30 s OFF for 5 min on a Bioruptor device (Diagenode) and centrifuged at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The chromatin solution was then sonicated for 15 cycles of 30 s ON and 30 s OFF using a Picoruptor Sonicator (Diagenode) to generate 200- to 500-bp DNA fragments ...
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bioRxiv - Systems Biology 2024Quote: ... The proteins were precipitated with acetone for 3 hours at −30°C and the resulting pellet was dispersed in 50 mM triethylammonium bicarbonate by ultrasonic treatment (three times for 30 s with intervals of 30 s) with a Bioruptor (Diagenode). The protein suspension was subjected to digestion with lysyl endopeptidase (Wako ...
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bioRxiv - Cancer Biology 2021Quote: ... 30 s off using a Bioruptor sonicator (Diagenode). For each immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 s off using a Bioruptor Plus (Diagenode). Lysates were cleared via centrifugation at 21,000g for 10 min at 4°C ...
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bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Molecular Biology 2024Quote: ... They were subsequently sonicated for 4 cycles (30 sec on, 30 sec off) using the Bioruptor Pico (Diagenode) and centrifuged for 15 min at 14,000 x g ...
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bioRxiv - Physiology 2024Quote: ... and 500 µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... After glass-bead beating, chromatin was sonicated for 24 cycles (high output, 30 s-on, 30 s-off) using the Bioruptor Plus (Diagenode, Belgium) to produce fragments of ∼ 250 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... the nuclear pellet was resuspended in 300 µl IP buffer and sonicated four times for 10 min (30 s on mode, 30 s off mode) using the Bioruptor Plus (Diagenode, Belgium). Sheared chromatin was collected by centrifugation and a 10-µg sample was used for IP ...
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bioRxiv - Biochemistry 2021Quote: ... and then sonicated for 30 min of 30 cycles (30 s run and 30 s rest) using the noncontact ultrasonic rupture device (Diagenode, Liege, Belgium). The resultant bacterial lysate was centrifuged twice at 12,000 rpm for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... cells were disrupted by sonication for 10 min (alternating 20 s off and 40 s off) using a Bioruptor Plus (Diagenode, Seraing, Belgium). Cell debris was removed by centrifugation at 14,000 rpm for 1 h at 4 °C and the supernatant was retained ...
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bioRxiv - Genetics 2022Quote: ... on ice for 6 min and then sonicated with 30 s ON and 60 s OFF for 16 cycles with Bioruptor Pico sonication device (Diagenode SA, B01060010) at 4 ℃ ...
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bioRxiv - Plant Biology 2022Quote: ... 1× protease inhibitor cocktail) and sonicated with 25 cycles of 30-s-long pulses (30-s intervals) using a Bioruptor Plus (Diagenode, Denville, NJ). Nuclear lysates were cleared with 2 rounds of centrifugation at 13,000 × g for 5 min at 4 °C ...