Labshake search
Citations for Greiner :
1401 - 1450 of 1462 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... and subsequently diluted 1:125 in culture medium and added to quadrant 1, 2 and 3 (respectively for 10mM, 1mM and 0.1mM stock plates) of deepwell 384-well plates (#781271, Greiner Bio-One). DMSO-stock solutions of control molecules (100% DMSO as negative control and as positive controls we included 1000-times concentrated docetaxel [30uM] ...
-
bioRxiv - Bioengineering 2022Quote: ... First plasma was collected as follows: 1 mL of blood was placed in K3-EDTA collection tubes (Greiner Bio-One, USA) and centrifuged at 3,000 RCF ...
-
bioRxiv - Microbiology 2022Quote: ... Spores (OD600 = ∼1, 150 μl/well) were added to a 96-well flat-bottomed microtiter plate (black wall, Greiner Bio-One), and the optical density and fluorescence intensity were measured by a BioTek plate reader ...
-
bioRxiv - Microbiology 2022Quote: ... seedlings were singularised and transferred to 1/2 Murashige and Skoog (MS) media in sterile 12-well plates (Greiner bio-one) and grown for three more weeks ...
-
bioRxiv - Immunology 2019Quote: ... Blood was collected using plain hematocrit tubes (Drummond; 1-000-7500-C/50) into K2-EDTA tubes (Terumo CapiJect; T-MQK or Greiner; 450480) and processed the same day ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... tRNAscan-SE was also used to identify tRNA genes in the mitochondrial contigs of the S. chalcedonica assembly. The mitogenome plot (Supp. Fig. 1) was produced with OGDRAW version 1.3.1 (Greiner, et al. 2019). The annotated A ...
-
bioRxiv - Molecular Biology 2023Quote: ... Serially diluted PFI-7 and GID4-Tracer at a concentration of 1 μM were pipetted into white 384-well plates (Greiner 781207) using an Echo acoustic dispenser (Labcyte) ...
-
bioRxiv - Microbiology 2023Quote: ... One milliliter of the solution containing the perturbation and 1 mL of the initial culture were combined in a 2-mL 96-deepwell plate (Greiner, 780270).
-
bioRxiv - Microbiology 2023Quote: ... One milliliter of the solution containing the perturbation and 1 mL of the initial culture were combined in a 2-mL 96-deepwell plate (Greiner, 780270).
-
bioRxiv - Biophysics 2022Quote: ... in PBS buffer was incubated with 25 μl of fresh stock of 3 mg/ml fluorescamine in DMSO at room temperature for 1 hour in a 96-well plate (Greiner 650209). Fluorescence intensity (λEx = 365 nm ...
-
bioRxiv - Microbiology 2023Quote: ... The cell-pellet was then resuspended in 1 ml of TR salts medium and 5 µl of this suspension inoculated into 195 µl TR medium with either carbon source (∼105 colony forming units (CFUs ml−1) in a 96-well plate (Greiner Bio). The optical density (600 nm ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... were washed twice with PBS and mixed together in 1 ml PBS in a 24-well plate (Greiner Bio-One GmbH) at a final OD600 of 0.7 for S ...
-
bioRxiv - Neuroscience 2021Quote: iAstrocytes and hiPSC-derived astrocytes generated according to TCW et al.18 were plated at 20,000 cells per cm2 onto a Greiner μClear 96-well plate (Greiner Bio-One cat. no. 655087) coated with 1:200 diluted Matrigel ...
-
bioRxiv - Cell Biology 2019Quote: ... At the second round cells were transfected with WT or Quad K Jam-C-GFPout and plated on borosilicate glass bottomed dishes (Greiner Bio One, Kremsmunster, Austria). The following day cells were imaged in the presence or absence of 100 nM Bafilomycin (Life technology ...
-
bioRxiv - Plant Biology 2020Quote: ... The stem piece was then placed onto a cell culture dish with a glass bottom of 175 μm (CELLview™ dish, Greiner Bio-One, Germany) in a drop of water and observed using the inverted microscope of a confocal laser scanning microscope (model TCS SP8 with a HyVolution super-resolution detector ...
-
bioRxiv - Immunology 2022Quote: ... Cells were individually trypsinized and combined according to the above-mentioned ratio and seeded in 96-well U-bottom cell repellant plates (Greiner Bio-One, Monroe, NC) in 75 μl of RPMI media per well ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were individually trypsinized and combined according to the above-mentioned ratio and seeded in a 96-well U-bottom cell repellant plate (Greiner Bio-One, Monroe, NC) in 75 µl of EGM-2MV media per well ...
-
bioRxiv - Cell Biology 2019Quote: Immunostaining of the cells 97 was performed in 96-well glass bottom plates (Whatman, GE Healthcare, UK, and Greiner Sensoplate Plus, Greiner Bio-One, Germany) coated with 50 μl of 12.5 μg/ml human fibronectin (Sigma Aldrich ...
-
bioRxiv - Microbiology 2020Quote: Anastomosis of conidia/conidial germlings by formation of CATs was routinely assayed in wells of either polystyrene 24-well cell culture plates (662160 from Greiner Bio-One, Kremsmünster, Austria) or glass-bottom 96-well plates (MGB096-1-2-LG-L from Matrical Bioscience ...
-
bioRxiv - Microbiology 2021Quote: J774A.1 mouse macrophage and LLC-PK1 porcine renal tubule epithelial cell lines were plated in 384-well tissue culture dishes with white walls and clear bottoms (Greiner, Monroe, NC, catalog #781098) at ∼7.0 × 105 cells/cm2 and ∼7.0 × 104 cells/cm2 ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were then counted and plated at a density of 2,000 cells/well on Greiner half-area 96-well plates with a cyclic olefin copolymer (COC) bottom (Greiner Bio-one, Item No.: 675801) in plating media ...
-
bioRxiv - Cancer Biology 2023Quote: HCT116 cells were plated (3 x 103 cells/well) and grown for 3 days in 96-wells ultra-low attachment plates (ref 650970, Greiner Bio-One, Les Ulis) before treatments ...
-
bioRxiv - Cancer Biology 2023Quote: ... Nuclei were isolated with NP40 lysis buffer with a 5 min incubation on ice and passed through a 70 μm cell strainer (Greiner Bio-One EASYstrainer, ThermoFisher). Samples were pooled based on nuclei concentration (Countess II cell counter ...
-
bioRxiv - Microbiology 2023Quote: ... Grown samples were diluted in the respective medium to 106 cells/ml and 150 μl (per well) was transferred to 96-well round (U)-bottom microtiter plate (MTP) (Greiner Bio-One, Kremsmünster, Österreich). To each well ...
-
bioRxiv - Bioengineering 2023Quote: ... 2 × 103 MDA-MB-231 breast cancer cells were mixed with 2 × 103 ADSCs and 200 µL of this cell suspension was seeded into each well of a U-bottom 96-well plate with cell-repellent surface for 24 h (Greiner bio-one, Frickenhausen, Germany) to form the spheroids ...
-
bioRxiv - Biochemistry 2024Quote: ... The cell suspension is filtered once more through a 70-µm strainer (into another 50 ml centrifuge tube to rinse and collect all the cells. 100-mm diameter suspension dishes (Greiner Bio-One, Monroe, NC) are seeded with 10 mL per dish at 0.6-0.8 x 106 cells/mL (∼10,000-15,000 cells/cm2) ...
-
bioRxiv - Biochemistry 2020Quote: ... 1-2 μl of beads per sample were pipetted into the wells of a 384-well glass-bottom microplate (Greiner Bio-One) pre-filled with a prep of ATTO390-SUVs in buffer containing 25 mM HEPES pH 7.5 ...
-
bioRxiv - Genomics 2020Quote: Overnight cultures were diluted 100-fold in 1 mL of LB in a 96-deepwell plate (Masterblock 96 well, 2ml, V-bottom plates by Greiner Bio-one). After 2.5 h at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... Confrontations were carried out by spreading mixtures of both interaction partners at prey-predator ratios of 10:1 on 20 x wMY agar-plates (120 x 120 x 17 mm, Greiner Bio-One). At indicated time points cells were washed off the plate and centrifuged at 3000 rpm ...
-
bioRxiv - Cancer Biology 2021Quote: ... Serially diluted inhibitor and Tracer molecule 19c at a concentration of 1 µM determined previously as the Tracer 19c KD,app were pipetted into white 384-well plates (Greiner 781 207) using an Echo acoustic dispenser (Labcyte) ...
-
bioRxiv - Microbiology 2022Quote: ... 100 μL of RPMI-1640 was added to all the wells of columns 1-9 and 12 of a flat-bottom 96-well microtiter plate (Greiner Bio-One). 200 μL of RPMI-1640 + 12.8 mg/mL BA was added to all wells of column 11 and 200 μL of RPMI-1640 + 64 μg/mL FLC was added to all wells of column 10 ...
-
bioRxiv - Immunology 2022Quote: ... NR-54970) were seeded at a density of 1×104 per well in half area 96-well black opaque plates (Greiner Bio-One) and were grown overnight at 37°C in a 5% CO2 atmosphere ...
-
bioRxiv - Plant Biology 2021Quote: Seedlings were initially grown on 1/2 MS plates for 3 days before being transferred to 96-well plates (655075, Greiner Bio-One) in 100 μL liquid MS for 5 days ...
-
bioRxiv - Plant Biology 2021Quote: Four-day-old seedlings growing on 1/2 MS plates were transferred into individual wells of a transparent 48-well tissue culture plate (Greiner Bio-One) containing 500 μL of liquid MS media with/without elicitor addition ...
-
bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris·HCl pH 7.4 and 1 mM DTT in a 20 µl volume in non-binding clear bottom 384 well plates (Greiner Bio-One, 781906). Compounds ...
-
bioRxiv - Synthetic Biology 2021Quote: ... the cultures were diluted 1:100 into 1 mL of LB/chloramphenicol medium with different concentration of bile salts in 96 deep-well plates (Greiner bio-one), incubated at 37°C with vigorous shaking for a further 4 hours and analyzed by flow cytometry ...
-
bioRxiv - Microbiology 2022Quote: ... Filtrate and diluted concentrate (1:100) were added to a black optical bottom 96 well plate (10 µL/well; Greiner Bio-One) in technical duplicate and each fraction was processed separately in the presence or absence of hydrolysis enzymes according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... a 1:1 volume of reagent and culture medium was added to each sample of a black 96 well plate (655892, Greiner Bio-one), shaken for 10 min at room temperature ...
-
bioRxiv - Biophysics 2023Quote: ... or DMSO (columns 1-2, 23-26, and 47-48) was dispensed into 1536 well black polystyrene assay plates (Greiner, Kremsmünste, Austria) using an Echo 550 liquid dispenser (Beckman Coulter ...
-
bioRxiv - Immunology 2023Quote: ... Antigen-coupled microspheres were washed and incubated with plasma samples at an appropriate sample dilution (1:50 for Isotypes and 1:100 for all Fc-receptors) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
-
bioRxiv - Cancer Biology 2024Quote: ... and HSJD-GBM-001 (GBM1) were cultured in suspension in low-attachment 250 mL/75 cm2 flasks (Greiner #658, Supp. Table 1). The medium for DIPG4 ...
-
bioRxiv - Microbiology 2020Quote: ... Vero E6 cells were seeded at 3×104 in 100 μl DMEM+10% heat-inactivated FBS in 96-well Operetta plates (Greiner Bio-One, Monroe, NC, USA). The following day ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2 mL and 40 mL scale transfections of HEK293E cells were performed in 6-well plates (Costar, Cat no. 3516) and T175 flasks (Greiner Bio-one, Cat no. 660175) respectively ...
-
bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated from EDTA anti-coagulated blood by density gradient separation using prefilled Leucosep™tubes (Greiner Bio-One Ltd, UK). PBMC were cultured in RPMI1640 containing 10% FBS and 1% Penicillin Streptomycin (PS ...
-
bioRxiv - Immunology 2021Quote: ... centrifuged at 300 x g for 10 minutes and then cultured at a density of 4×105 cells/well in a 24-well low adherence culture plate (Greiner Bio-One, North Carolina, USA). Both “Uninfected” and “Infected” wells were seeded for comparison between infection states ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Biological activity of the virus from the lung samples was determined by titrating the prepared suspension to a monolayer of Vero C1008 cell culture in plastic vials with a 25 cm2 working surface area (CellStar, Greiner Bio-One GmbH, Kremsmünster, Austria). After removing the medium ...
-
bioRxiv - Microbiology 2023Quote: BMDMs were cultured as described before and were seeded at a density of 106 cells/ml in 24-well plates (Greiner Bio-One, Kremsmu□nster, Austria). The cells were challenged with the conidia of different strains at a multiplicity of infection of 1:10 and incubated a 37°C with 5 % (vol/vol ...
-
bioRxiv - Molecular Biology 2021Quote: ... and mixed with biotinylated RBD protein (final concentration 1 nM) in white low binding 384well microtitre plates (F-bottom, Greiner Cat nr 781904). As isotype control antibody ...
-
bioRxiv - Pathology 2021Quote: ... diluted to a concentration of 1 mM and 40 nL were dispensed in 384 well plates (Greiner µClear #781091, Greiner Bio-One GmbH) using an acoustic liquid handler (Echo® 550 ...
-
bioRxiv - Pathology 2021Quote: ... diluted to a concentration of 1 mM and 40 nL were dispensed in 384 well plates (Greiner µClear #781091, Greiner Bio-One GmbH) using an acoustic liquid handler (Echo® 550 ...