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Citations for Greiner :
1301 - 1350 of 1462 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... cells from overnight culture were diluted to OD0.1 in LB and transferred to 96-well black and clear-bottom CELLSTAR® format sterile cell culture microplates (Greiner Bio-One) with 3 technical replicates ...
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bioRxiv - Immunology 2022Quote: ... Purified naive or memory CD4+ or CD8+ T cells were activated for indicated time points in 48-well culture plates (Greiner, Monroe, NC) at 2.5 – 3.0 × 105 cells/well in media containing 25 U/ml recombinant human IL-2 in an incubator at 37°C and 5 % CO2 ...
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bioRxiv - Pathology 2022Quote: ... 150,000 cells/cm2 were seeded with or without tetracycline (5 µg/ml) for 6 days on 6 well plate (Greiner, 657 160). Cells were lysed in Pierce NE-PER™ buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Damage was created by exposure to a UV-A laser beam in cells plated on glass-bottomed dishes (Greiner Bio-One 543979). Cells were pre-sensitized with 1 ng of Hoescht in normal medium for 20 minutes at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 200 μL of cells were then added to individual wells in black clear-bottom 96-well microplates (Greiner 655087 or Corning 3631). Nitrogen-starved cells served as a reference treatment for autophagy ...
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bioRxiv - Plant Biology 2021Quote: ... 200 μl of yeast suspension were added to 800 μl of 0.2x PDB (final OD600 = 0.004) and grown in polystyrene 48-well plates (CELLSTAR® Cell Culture Multiwell Plates, TC treated, Greiner Bio-One) without shaking ...
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bioRxiv - Systems Biology 2019Quote: ... Confocal high-resolution 3D imaging of the PCLS was accomplished by placing the PCLS into a glass-bottomed 35 mm CellView cell culture dish (Greiner BioOne, 627870) as a wet chamber ...
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bioRxiv - Biochemistry 2019Quote: ... dissolved in DMSO was added to 198 µl of cell culture in a black f-bottom 96 well plate (Greiner bio-one) and incubated at room temperature in the dark for 60 minutes before recording fluorescence at 520 nm after excitation at 485 nm in a FluoStar Optima Spectrophotometer (BMG labtech) ...
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bioRxiv - Plant Biology 2019Quote: ... were vapour sterilized and sown in modified KNOP medium (Sijmons et al., 1991) in 6-well cell culture plates (Greiner Bio-one). Seedlings were grown at 21°C under a 16-h/8-h light/dark regime ...
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bioRxiv - Cell Biology 2019Quote: ... Glomeruli were then cultured for 7 days at 37°C in the presence of 5% CO2 in Petri dishes (145/20 mm, Cell Star; Greiner Bio-One) coated with collagen I (Merck) ...
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bioRxiv - Bioengineering 2020Quote: ... The mixed composites were then molded in polystyrene cell culture inserts (0.4 μm Polyethylene terephthalate; PET, membrane, 12 well plate diameter, Greiner bio-one, Austria) and in turn frozen at −80°C for 10 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... Each assembled cell-free reaction was 10 μL in volume and placed in a black-bottomed 384-well plate (Greiner Bio-One) and incubated at 37 °C for 3 hours for GFP expression ...
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bioRxiv - Developmental Biology 2020Quote: ... The left- and right-wing discs of each individual were mounted without coverslip on “Cellview” cell culture dishes with glass bottom (Greiner Bio-one), in order to preserve the original structure volume ...
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bioRxiv - Microbiology 2019Quote: ... Aliquots of 180 µL of cell suspension were transferred to a black flat-bottomed 96-well plate (Greiner Bio-one, Stonehouse, UK). Eight technical replicates ...
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bioRxiv - Systems Biology 2021Quote: hCMEC/D3 cells were cultured to confluency on transwell inserts (0.4 µm pore size, 0.33 cm2 diameter, Greiner Bio-One Gmbh, Austria) prior to 16 h treatment with 10 ng/ml TNFα ...
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bioRxiv - Cancer Biology 2020Quote: ... a 30 μl aliquot of each single cell line at density of 75,000 cells/well was delivered to a well in a 384-well microtiter plate (Greiner Bio-One 781091) pre-coated with 10 μg/ml of Rat Tail Collagen Type I (354249) ...
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bioRxiv - Microbiology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood samples by Ficoll density gradient centrifugation using LeucoSEP tubes (Greiner Bio-One) as described previously (96) ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of DMSO solutions was spiked into dry well of F-bottom clear cell culture treated 96-wells plates (Greiner Bio One), with columns 1 and 12 devoted to controls and used to calculate the Z’-factor ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 cells (2.5 × 104) were seeded in each well of black CLEAR flat-bottom 96-well plate (Greiner Bio-one™). The cells were incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... were produced by inoculation of VeroE6/TMPRSS2 cells and titred as previously described.(33, 36) Caco-2-ACE2 cells and cardiac PCs were plated in μClear 96-well microplates (Greiner Bio-one) and the next day infected with either REMRQ0001 or B.1.1.7 at multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2022Quote: RPE1 cells stably expressing CENP-A-GFP and Centrin1-GFP and HeLa cells stably expressing PRC1-GFP were grown on six-well plates (Greiner Bio-one) and subjected to HAUS6 or HAUS8 siRNA treatment as indicated before ...
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bioRxiv - Bioengineering 2022Quote: ... B6 patellar tendon cells were seeded (7,500 cells/well) in basal media onto B6 naïve or provisional ECM-coated 96-well glass-bottom plates (Greiner Bio-One, #655892). Non-coated wells served as controls ...
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bioRxiv - Cancer Biology 2022Quote: ... The BME-cell suspension was seeded as 30 μL drops on prewarmed 24-wells suspension culture plates (Greiner Bio-One, Kremsmünster, Austria) and cultured following standard protocols28.
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bioRxiv - Genomics 2022Quote: ... 120 μL of a suspension containing 4×105 cells from each sample was then plated in a 96-well untreated plate (Greiner Bio-One) for each of the three sets of stimulation conditions ...
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bioRxiv - Microbiology 2022Quote: ... for 7 hours at 37 °C and added to fully confluent A549 cells in a 24-well glass bottom plate (Greiner Bio-one). Germlings for the TF mutants with a delay in germination were grown for a longer length of time until they achieve approximately similar visual hyphal growths as the parental strain ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were grown to confluence before transferring to a 12-well plate with ThinCert™ cell culture inserts with 0.4μm pores (Greiner Bio-One, 665641). Cells were airlifted after 2-3 days by removing the medium from the apical chamber and providing PneumaCult-ALI media (StemCell ...
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bioRxiv - Immunology 2022Quote: The blood from a ImmunoVax study donors were collected in EDTA tubes and the isolation of blood mononuclear cell was performed using Leucosep centrifuge tubes (Greiner Bio-one) prefilled with density gradient medium (Ficoll-PaqueTM PLUS ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell culture was supported by a fully automated cell culture device (SelecT, TAP_UK) and screening was done in 1536 well plate assay format (Greiner, Cat. #789183-A). Cell passaging was done every 3rd ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were then agitated for 5 min and 5 μl of the cell lysate was transferred to a white 384-well ProxiPlate (Greiner Bio-one) followed by the addition of 5 μl of the detection buffer (a mixture of activation buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4 µL of HepG2-CYP2B6-hCAR cells at 6.25 × 105 cells/mL (2,500 cells/well) were dispensed using a Multidrop Combi dispenser in tissue culture–treated 1536-well white assay plates (Greiner Bio-One) in the same culture media ...
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bioRxiv - Molecular Biology 2023Quote: Stably integrated optoDroplets HeLa cell lines (listed in Table S9) were seeded on the 35-mm glass-bottom dish (CELLview™ Culture dish, Greiner, 627860) and grown overnight in normal growth medium to reach ∼ 50% confluency ...
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bioRxiv - Cancer Biology 2022Quote: ... This cell suspension was passed through a 70 μm filter (#352350; Falcon) and seeded on a treated cell culture flask (T-75 #658175; Greiner Bio-One). NPSF cultures were incubated in a humidified 32 °C incubator with 5% CO2 and 3% O2 ...
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bioRxiv - Molecular Biology 2023Quote: ... embryoid bodies (EBs) were generated by growing 750’000 cells in suspension for 6 days in non-adherent dishes (Greiner Bio-One 633181). Spheroids were allowed to grow without disturbing the plate for the first 3 days ...
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bioRxiv - Pathology 2023Quote: ... cells were seeded in a volume of 40 μl/well in 96 well plates (black with micro-clear bottom) (Greiner Bio-one). For western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... BRAF-mutant VM48 melanoma cells (1,500 per well) were seeded in a round-bottom 96-well plate (Greiner Bio-One, Monroe, NC) in full RPMI containing 0.3% methylcellulose (4,000 cP ...
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bioRxiv - Genetics 2022Quote: ... Transfected cells were washed twice with 2 mL of extracellular solution (ECS, see below) and re-plated onto 35-mm polystyrene cell culture dishes (Cellstar, Greiner Bio-One). hKV1.3 currents were recorded from activated peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Microbiology 2023Quote: 25000 A549 or 30000 HuTu80 cells per well were seeded in a black optical bottom 96-well plate (Greiner Bio-one; #655096) 24 h prior to experimentation ...
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bioRxiv - Cell Biology 2023Quote: WT SH-SY5Y cells or those expressing GFP-γ-tubulin/LGP120-mCherry were cultured in 96-well black plates (Corning: 3603 or Greiner: 655892). After 48 hours of culture ...
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bioRxiv - Immunology 2023Quote: ... Ex vivo PBMC stimulations were performed with 5×105 cells/well in round-bottom 96-well plates (Greiner Bio-One, Kremsmünster, Austria) for 24 hours at 37°C and 5% carbon dioxide ...
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bioRxiv - Microbiology 2023Quote: ... Immediately after transfection 10,000 cells were seeded into flat bottomed 96-well tissue culture plates or clear 96-well Microplates (Greiner Bio-one, UK) containing 10 μM mα-DGN ...
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bioRxiv - Cell Biology 2024Quote: HEK293T cells expressing HA-TC-hGAPDH protein with or without FLAG-IDO1 in black walled 96-well plate (Greiner Bio-One # 655090) were stained with FlAsH-EDT2 ...
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bioRxiv - Bioengineering 2024Quote: ... Spleens were manually homogenized by the plunger of a 3 ml syringe (Becton, Dickinson & Company) in a 100 μM cell strainer (Greiner Bio-One). Single cell suspensions of splenocytes were prepared by forcing cells through cell strainers with complete RPMI media (RPMI ...
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bioRxiv - Neuroscience 2024Quote: Part of the HEK293 cells transfected described above were also transferred in a 96-well plate (Greiner bio-one, PS, F-bottom), 105 cells per well ...
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bioRxiv - Immunology 2024Quote: ... blood samples were collected from participants in sodium heparin or lithium heparin Vacutainer CPT Mononuclear Cell Preparation Tubes (BD Biosciences or Greiner Bio-One). PBMC were isolated by density centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed using 1x passive lysis buffer and transferred to a solid white flat-bottom 96-well plate (Greiner Bio-One). Firefly luciferase activity was read first on a GloMax Multi Detection System (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed and incubated with D-Luciferin substrate for 5 min in white Lumitrac™ plates (Greiner Bio-one, Monroe, NC). Luminescence was analyzed using a SpectraMax M5 system connected to SoftMax Pro version 8.0 software (Molecular Devices ...
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bioRxiv - Microbiology 2024Quote: ... Vero E6 cells (2.5 × 104) were seeded in each well of black μCLEAR flat-bottom 96-well plate (Greiner Bio-one™). The cells were incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... and 1:100 for all other readouts) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). The high affinity FcR was not tested due to its minimal role in tuning antibody effector function 48 ...
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bioRxiv - Developmental Biology 2021Quote: Embryos were anesthetized and mounted in 1% low-melting agarose on a 35-mm-diameter glass-base dish (627870 or 627861 Greiner). Confocal images were obtained using a Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were mounted with 10 μL of 1% agarose gel onto a glass-based dish (Greiner Bio-One, #627871) for stable imaging ...