Labshake search
Citations for Greiner :
1351 - 1400 of 1462 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... The samples were mounted with 10 µL of 1% agarose gel onto a glass-based dish (Greiner Bio-One, #627871) for obtaining stable images ...
-
bioRxiv - Immunology 2022Quote: ... and 1:100 for all other readouts) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... LC fractions (1 per 6 s) were collected row by row in serpentine-like fashion on clear 384-well plates (Greiner Bio One ...
-
bioRxiv - Immunology 2019Quote: ... were isolated by resuspending cells in 4 mL P80 (80% P100 (9:1 percoll:10X PBS) + 20% RPMI) percoll in a 15 mL centrifuge tube (Greiner) before overlaying 4 mL P40 (40% P100 + 60% 1X PBS ...
-
bioRxiv - Immunology 2019Quote: Collected blood was placed in 1 mL of sterile 2 mM EDTA/PBS solution in a 15 mL centrifuge tube (Greiner). Tubes were topped up with ice-cold PBS + 0.1% BSA ...
-
bioRxiv - Evolutionary Biology 2020Quote: Overnight cultures were diluted 1:103 in fresh competence medium and grown for 4.5 h (37°C, 250 rpm) in a 24-well microtiter plate (1 mL, final OD ≈ 0.25; Greiner Bio-one). Microtiter plates were covered with rayon film adhesive covers (VWR) ...
-
bioRxiv - Immunology 2021Quote: ... infected larvae at 2 dpi were mounted in 1% low-melting point agarose (Fisher BioReagents) in a 35mm glass-bottom dish (Greiner) and oriented laterally ...
-
bioRxiv - Immunology 2021Quote: ... and 1:100 for all other readouts) for 2 hours at 37°C in 384-well plates (Greiner Bio-One). Unbound antibodies were washed away ...
-
bioRxiv - Cell Biology 2022Quote: ... 4×104 ECs per well were seeded for confluent experiments and 1×103 ECs per well for sparse experiments in fibronectin-coated 96-well plates (μClear, Greiner). Cells were cultured for 24h for sparse and 96h for confluent under basal conditions (EGMV2 ...
-
bioRxiv - Microbiology 2022Quote: ... The antigen-coupled microspheres were washed and incubated with heat-inactivated serum samples at an appropriate sample dilution (1:100-1:400 for antibody isotyping and 1:1,000 for all low-affinity FcγRs) overnight in 384-well plates with continuous shaking (Greiner Bio-One). Unbound antibodies were washed away using the magnetic 384-well HydroSpeed Plate Washer (Tecan ...
-
bioRxiv - Biochemistry 2022Quote: ... 150 μl of the resulting mixture was pipetted into 1 well of a 96-well plate (Greiner Bio-One, # 655076). CaM was used at a final concentration of 673 nM (13 ng/μl) ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 μl of respective beads was transferred into the well of a 384-well glass-bottom microplate (Greiner Bio-One) pre-filled with 20 μl of 25mM TRIS-Cl pH 7.5 ...
-
bioRxiv - Microbiology 2023Quote: ... 180 μl M9 minimal medium containing kanamycin and gentamicin as well as purine compounds at concentrations from 0 to 1 mM were added into 96-well black microplates (Greiner). Subsequently ...
-
bioRxiv - Microbiology 2024Quote: ... virus solutions were manually mixed immediately prior to deposition of 1 μL droplets on open 96-well plates (non-binding microplates, flat-bottom, transparent, Greiner Bio-One ...
-
bioRxiv - Biophysics 2021Quote: TIRF was used to monitor serum-induced detachment of LNPs tethered to the surface of CELLview™ glass-bottom quartering cell culture dishes (Greiner Bio-One) via a streptavidin-biotin linkage to DSPE-PEG2000 biotin lipids (0.006% incorporation) ...
-
bioRxiv - Microbiology 2021Quote: ... 10 µl of the cells were transferred to different wells of a 96-well plate (Greiner Bio-One™ CellStar™ μClear™) containing 140 µl of MSM with elevated ferulate concentrations (20 mM – 80 mM) ...
-
bioRxiv - Cell Biology 2022Quote: 2’500 siRNA transfected cells were seeded 24h after transfection per well in 100 µl of standard growth media in cell-repellent 96 well microplates (Greiner Bio-one, 650790). Plates were incubated 24 hours at 37°C in 5% CO2 to allow spheroid formation ...
-
bioRxiv - Bioengineering 2020Quote: RAW 264.7 macrophages were seeded at 50,000 cells per well in a 96 well black glass-bottom plate (Greiner 96 well plates, 655891, VWR). Cells had been previously incubated with folic acid deficient RPMI medium (10% FBS ...
-
bioRxiv - Neuroscience 2021Quote: ... seeded (9,000 cells/well) onto poly-L-ornithine/ laminin-coated 384-well optical bottom plates containing the kinase inhibitors (Greiner Bio-One, Kremsmünster, Austria) and cultured in neuronal differentiation medium for two weeks (Fig ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were then transferred to a sterile Cellstar T-75 culture flask with a red filter screw cap (Greiner Bio-One, Monroe, NC) containing RPMI 1640 (with glutamine and phenol red ...
-
bioRxiv - Cell Biology 2021Quote: RPE1 cells with stable expression of H2B-mTurquoise and Geminin-mVenus were seeded in 96-well μclear microplates (Greiner Bio-one, Monroe, NC) and grown in the incubator for at least 6 hr prior to imaging ...
-
bioRxiv - Biophysics 2020Quote: ... One day before the stress granule co-localization assay and FRAP measurements cells were seeded in 96-well CELLview™slides (Greiner Bio-One) with glass bottom suitable for imaging so that on the day of the measurement cells reached ∼50% of confluence ...
-
bioRxiv - Cell Biology 2021Quote: Primary rat cardiac cells (Innoprot, Elexalde Derio, Spain) were cultured on gelatin-coated 75 cm2 flasks (CELLSTAR, Greiner Bio-One GmbH, Frickenhausen, Germany) in DMEM/F12 medium supplemented with 20% FBS ...
-
bioRxiv - Microbiology 2022Quote: ... 1.2 ×104 Vero cells were plated into each well of a black clear flat-bottom 96-well plate (Greiner Bio-One; Cat# 655090). The cells were incubated overnight at 37 °C with 5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: ... 250,000 epithelial progenitor cells were detached from feeders as described above and seeded onto matrigel coated 24 well inserts (ThinCerts™, Greiner Bio-one) and fed apically and basally with BEGM Bronchial Epithelial Cell Growth Medium Bullet Kit (Lonza ...
-
bioRxiv - Immunology 2021Quote: ... and CHO wt cells transiently expressing CD4 domain-deletion mutants were plated at ∼10,000 cells per well of a µClear 96-well plate (Greiner Bio One, cat. #655090) and cultivated at standard conditions ...
-
bioRxiv - Cancer Biology 2021Quote: ... MCF7 or BT549 cells with or without genetic modifications and transduced with an H2B-mCherry construct into a 4-section imaging chamber (Greiner Bio-one,627870). Cells were grown overnight and treated with mock-control or 250 nM reversine 1 hour before imaging started ...
-
bioRxiv - Cancer Biology 2021Quote: HEp-2 cells conjugated to NanoShuttlesTM were seeded on a 24-well ultralow-attachment plate (ULA, Cellstar® Greiner Bio-one, Kremsmünster, Austria) in 105 cells and a final volume of 400 μL/well.
-
bioRxiv - Cancer Biology 2019Quote: HEK cells grown in flasks were trypsinized and seeded at a density of 0.25 × 106 cells in (tissue culture grade) 35mm culture dishes (Greiner Bio-one, Kremsmünster, Austria). 100nM siRNA or plasmids at indicated concentrations were transfected with Dharmafect or Liofectamine 2000 as per the manufacturer’s instructions when cultures were 50-60% confluent (by 24 h post-plating) ...
-
bioRxiv - Bioengineering 2021Quote: ... The mononuclear fraction of CB and AML cells were isolated by using a Ficoll density centrifugation with Lymphoprep (Axonlab, 1114545) in Leucosep tubes (Greiner bio-one, 227290). Prior to culturing or freezing AML cells ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cell mixture was suspended in the SSFG medium and dispensed into the round-bottom non-treated 96-well plate (Greiner bio-one, 650185). The plate was centrifuged at 400x g for 3 min to facilitate aggregation and incubated for 7 days ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 x 105 MDA-MB-231 cells were plated on a 6-well insert with a polycarbonate membrane pore size of 8 µm (Greiner Bio One 657628) and the cells were recovered and analysed 48 h later.
-
bioRxiv - Microbiology 2021Quote: ... Intermediary compound dilutions were made in complete cell medium directly before adding the compound to 96-well plates (CELLSTAR, Greiner-Bio, Vilvoorde, Belgium) in which Vero E6-EBOV-VP30 cells had been seeded one day prior at 20,000 cells/well ...
-
bioRxiv - Immunology 2022Quote: Bone marrow exudate cells were recovered from tibias and femurs of C57BL/6 mice and cultured in bacteriological Petri dishes (Greiner bio-one 664161) in DMEM medium (Gibco Life Technologies ...
-
bioRxiv - Immunology 2022Quote: ... were plated at a density of 5 × 103 cells per well in white-walled 96-well tissue culture plates (Greiner Bio-One 655098) in growth medium (DMEM ...
-
bioRxiv - Plant Biology 2022Quote: ... The lysed cell contents were centrifuged at 1,000g for 3 minutes and divided into a white 96-well plate (Greiner Bio-One, Cat. # 655075) in triplicate ...
-
bioRxiv - Immunology 2022Quote: ... BM cells were plated at a density of 7 × 106 cells per well on a non-tissue culture polystyrene 94 mm petri dish (Greiner Bio-One, Germany) and cultured in 10 mL RPMI medium (Gibco™ ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... The cuvette was then incubated on ice for 10 min and cells were transferred to a 10-cm Petri dish (Greiner Bio-One #664160) containing 10 mL HL5 medium supplemented with heat-killed Klebsiella aerogenes (lab stock) ...
-
bioRxiv - Genomics 2023Quote: ... of U-251 MG dCas9-VPR were seeded in 30 µl of medium into white 384-well cell culture plates (Greiner Bio-One, #781080). The plates were incubated in a rotating tower incubator (LiCONiC StoreX STX ...
-
bioRxiv - Molecular Biology 2023Quote: ... at a final concentration of 0.2% v/v and immediately transferred onto a 10-well cell culture microscopy slide (CellView; Greiner Group AG, Kremsmünster, Austria) pre-treated with poly-lysine (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... WT and UBAP2L KO HeLa cells expressing GFP-FXR1 were grown on 35/10 mm 4 compartment glass bottom dishes (Greiner Bio-One, 627871) and synchronized by double thymidine block ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... This crucial assumption was based on the property of mutual exclusion (all depolarized cells are infected by max. one virion, Greiner et al. 2009), and was supported by single-cell burst size data for a related chlorovirus (Lievens et al ...
-
bioRxiv - Immunology 2023Quote: ... 1,000,000 cells in 2 ml culturing media were used for the 25 mm diameter coverslips (in a 6-well cell culture plate Greiner Bio-One #657160) and 200,000 cells in 200 µl culturing media were added to the apical compartment of transwell (the basolateral compartment of transwells was filled with 600 µl culturing media) ...
-
bioRxiv - Cancer Biology 2023Quote: The migratory ability of MCF7 and T47D cells transfected with SVIP siRNA was assessed using 24-well Transwell inserts (8-μm pore size; Greiner Bio-one, Austria). A total of 1×104 cells were plated in the upper chambers of Transwell filters in 100 μl phenol red free DMEM with 5% DCT-FBS ...
-
bioRxiv - Genetics 2023Quote: ... at 0.33 x 107 cells per well for the RNA sequencing experiment and 2 x 105 cells per well in a 96-well (M0562, Greiner, Sigma Aldrich, USA) for microscopy ...
-
bioRxiv - Neuroscience 2024Quote: ... HEK-M8 cells were seeded in Poly-L-Lysine HBr coated 96-well black wall/clear-bottom plates (Greiner Bio-One, Frickenhausen, Germany) at a density of 100,000 cells per well in normal cell culture medium and incubated overnight ...
-
bioRxiv - Genetics 2024Quote: ... the N/TERT-2G heterogeneous cell population was separated using the standard limiting serial dilution method in a 96-well plate (Greiner Bio-One, France), S1F/TERT-1 and Mel-ST heterogeneous cell populations were sorted using BD FACSMelody™ Cell Sorter ...
-
bioRxiv - Developmental Biology 2023Quote: ... the pieces were manually dissociated into single cells by brief pipetting and then transferred into wells of a 24-well glass bottom plate (Greiner Bio-One, 662896) that had been pre-coated with Protein A from Staphylococcus aureus (Sigma P6031 ...
-
bioRxiv - Cancer Biology 2022Quote: ... This mixture (1 mL) was applied to the bottom of an ultra-low attachment 6-well plate (Greiner Bio-One Inc.) and allowed to cool for 5 minutes at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 g (corresponding to approximately 8 pieces) of tissue was transferred to a vial for cryopreservation (Greiner Bio-One, Kremsmünster, Austria), covered with medium for cryopreservation composed of MEM alpha (Thermo Fisher Scientific ...