Labshake search
Citations for Stemcell Technologies :
401 - 450 of 574 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... in embryoid body (EB) Seeding medium with 50 μM ROCK inhibitor (Y27632, Stem Cell Technologies). On day 7 EBs were transferred from AggrewellTM800 24-well plates using wide-bore p1000 pipette tips and embedded in Matrigel by gently mixing around 30 EBs in 100 μl Expansion medium with 150 μl cold Matrigel (Corning ...
-
bioRxiv - Bioengineering 2021Quote: ... the cuvettes were allowed to rest for 15 minutes before the T cells were gently moved to antibiotic-free medium containing 1µg/mL DNase (StemCell Tech) at 37°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
-
bioRxiv - Neuroscience 2022Quote: iPSCs were cultured in feeder-free conditions on Matrigel-coated (0.08 mg/well) 6-well tissue culture plates in mTesR1 media (StemCell Technologies) at 37 °C and 5 % CO2 ...
-
bioRxiv - Genomics 2023Quote: Unedited and WDR90 knock-out iPSC clones were transitioned into feeder-free condition in mTeSR™ Plus medium (STEMCELL Technologies) in a matrigel-coated 6 well plate at a density of 5x105 cells/well with 5 µM ROCK Inhibitor ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
-
bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
-
bioRxiv - Immunology 2019Quote: ... Primary human NK cells were isolated from leukocyte-enriched peripheral blood of healthy human donors using the RosetteSep™ Human NK Cell Enrichment Cocktail (StemCell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Primary human CD8+ were isolated from healthy donor blood with commercially available negative selection kits (RosetteSep Human CD8+ T cell Enrichment Cocktail, STEMCELL technologies, UK) following the manufacturers instruction ...
-
bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
-
bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
-
bioRxiv - Cell Biology 2022Quote: ... lineage depletion was performed as follows: added 20 µl of the EasySep mouse hematopoietic progenitor cell isolation cocktail (Stem Cell Technologies 19856), incubated for 15 min ...
-
bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
-
bioRxiv - Cell Biology 2023Quote: Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). T cells were cultured at 37°C and 5% CO2 in RPMI 1640 (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... Human CD4+ T cells were isolated from approximately 2 x 108 elutriated lymphocytes to approximately 90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (15062; Stem Cell Technologies), using Ficoll/Hypaque (45-001-749 ...
-
bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
-
bioRxiv - Immunology 2024Quote: Human T cells were isolated from healthy donors who provided informed consent using RosetteSep Human T-Cell Enrichment Cocktail (STEMCELL Technologies, Canada) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2024Quote: ... NK cells were isolated from whole blood using a density based negative selection kit according to the manufacturer’s instructions (RosetteSep Human NK Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation ...
-
bioRxiv - Bioengineering 2024Quote: ... CD3+ primary human T-cells were isolated by negative selection as per manufacturer’s instructions (Cat # 15021, 15061, RosetteSep Human T-cell Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). T-cell pellets were resuspended in dilution medium and counted using a hemocytometer with 0.4% Trypan blue viability stain (ThermoFisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... each containing 80µL protease mix (4.5µL/mL Accutase; Stem Cell Technologies, 07920; 4.5 µL/mL Accumax; Stem Cell Technologies, 07921 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Dual SMAD inhibitors (2μM Dorsomorphin; StemCell Tech; #72102, and 2 μM A83-01; StemCell Tech; #72022) until day 5 ...
-
bioRxiv - Cell Biology 2021Quote: ... and then replated in media supplemented with 10 µM ROCK inhibitor (Y-27632, StemCell Technologies, #72304). For the transduction of the virus ...
-
bioRxiv - Cell Biology 2022Quote: ... medium was supplemented with 20% Wnt3a-CM and 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). For the first day ...
-
bioRxiv - Bioengineering 2021Quote: ... cells grown in a culture dish were incubated with ROCK inhibitor (Y-27632, 10μM, StemCell Technologies) for 1 h at 37°C prior to harvesting with Accutase ...
-
bioRxiv - Cancer Biology 2023Quote: ... The electroporated cells were cultured in mTeSR Plus medium containing ROCK inhibitor (Stemcell Technologies, Cat#72304) for 24 hours after the electroporation ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were treated overnight with 10 µM Rock inhibitor Y-27632 (Cat. # 72304, Stem Cell Technologies) to attenuate cell death ...
-
bioRxiv - Developmental Biology 2024Quote: ... 10 μM ROCK inhibitor Y-27632 (Stem Cell Technologies, cat.# 72304; 10 mM stock in ddH2O), and 2.5 μM CHIR99021 (Stem Cell Technologies ...
-
bioRxiv - Systems Biology 2024Quote: ... resuspending in mTESR supplemented with 10uM Y-27632 dihydrochloride ROCK inhibitor (Stemcell Technologies; Cat. No. 72302) and plating in Geltrex-coated plates.
-
Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... complete solution was removed and 3 ml 2i+Lif medium + 10uM ROCK inhibitor (Stemcell Technologies, 72304) was added ...
-
bioRxiv - Immunology 2019Quote: Cells were cultured at 37°C under 5% CO2 in 100 μl of StemSpan serum-free expansion medium (STEMCELL technologies 9650) supplemented with cytokine cocktails aimed at proliferation (50 ng/ml murine recombinant thrombopoietin (TPO ...
-
Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... between 50-250*103 cells per 200µl of the following culture media: Human LT-HSCs (LIN−/CD38lo/CD34+/CD45RA−/CD90+) in StemSpan Serum-free expansion medium (SFEM; Stemcell Technologies) supplemented with 100ng/ml human SCF ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissues were cut into 1-2 mm pieces and subsequently incubated in serum-free medium containing collagenase/hyaluronidase (Stem Cell Technologies) at 37 ºC ...
-
bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... CD34+ cells from MF patients were isolated as described above and cultured in StemSpan serum free medium (SFEM; cat# 09605, StemCell Technologies) with stem cell factor (SCF ...
-
bioRxiv - Cancer Biology 2021Quote: ... Primary patient material was aliquoted into 24- or 48-well plates in 200μl StemSpan™ Serum-Free Expansion Medium (SFEM) (STEMCELL Technologies) supplemented with 20% FCS and the following cytokines ...
-
bioRxiv - Cancer Biology 2022Quote: CD34-positive and Lin-negative cells from PBMC were single-cell sorted into 96-well plates containing 100 μL of free medium consisting of StemSpam SFEMII (Stemcell Technologies) with human cytokines SCF ...
-
bioRxiv - Genetics 2022Quote: ... HSPCs were transferred into erythroid differentiation medium (EDM) consisting of StemSpan serum-free expansion medium II(SFEM II; Stem Cell Technologies) with 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The CHOP-WT10 cell line was maintained as an iPSC culture35 and subsequently transitioned to feeder-free cultures and maintained with mTeSR1 (StemCell Technologies) on hESC qualified matrigel (Corning) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
-
bioRxiv - Genomics 2023Quote: ... The iPSCs were transitioned into feeder-free conditions inhouse and maintained as feeder-free cultures in mTeSR Plus basal medium supplemented with mTeSR Plus 5X supplement (StemCell Technologies). The cultures were grown in multiwell plates coated with Matrigel® hESC-Qualified Matrix (Corning ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then passaged using GCDR and replated on 10-cm dishes coated with Animal Component Free (ACF) Attachment Substrate (StemCell Technologies); all subsequent passages were grown using the same medium and culture substrate ...
-
bioRxiv - Cell Biology 2022Quote: ... were obtained from the CCHMC Pluripotent Stem Cell Facility (PSCF) (44) and cultured in feeder-free condition with mTeSR1 (85850, StemCell Technologies) and on a hESC-qualified Matrigel (354277 ...
-
bioRxiv - Genomics 2023Quote: ... hESC was maintained and expanded in feeder-free culture conditions with mTeSR basal medium (STEMCELL Technologies Inc., Vancouver, Canada, cat # 85850) on 6-well plates culture-treated coated with Corning Matrigel hESC-qualified Matrix ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were washed and resuspended in a fresh pre-warmed complete culture medium and cultured in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
-
bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 x 106 lineage negative cells/ml were transduced with 1 x 108 TU/ml of empty vector or Vegfc lentivirus in serum-free Stem-Span medium (StemCell Technologies) supplemented with 10 ng/ml IL-3 ...
-
bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...
-
bioRxiv - Cell Biology 2023Quote: For cell growth assay 100 LIN-CD34+CD38- CD9+ or CD9- cells were sorted and seeded in 96 well plate and culture in StemSpan Serum –Free Expansion Medium (SFEM, StemCell Technologies) supplemented with 100u/ml penicillin (Hyclone ...