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Citations for Stemcell Technologies :
351 - 400 of 574 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Monocytes were isolated from whole blood by negative selection using RosetteSep human monocyte enrichment cocktail following the manufacturer’s protocol (StemCell Technologies). Using density gradient medium (Lymphoprep ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Biophysics 2020Quote: ... The cells were resuspended in 1 mL of medium contained 1x Stem Span Cytokine Cocktail CC100 (Stem Cell Technologies, Canada) in 24-well plate ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from elutriated lymphocytes by negative selection using RosetteSep CD4+ T cell enrichment cocktail (StemCell Technologies) followed by Ficoll/Hypaque (Amersham Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies # 15061) or the EasySepTM Human Naïve CD4+ T cell Isolation Kit (Stem Cell Technologies #17953 ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary NK cells were isolated from peripheral blood by negative selection using RosetteSep human NK enrichment cocktail (Stemcell Technologies, 15065). Blood was layered onto a Ficoll-Paque (Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... blood was resuspended in PBS/2%FBS/EDTA and separated using Ficoll-Paque Plus (Cytiva, 17144002) density gradient centrifugation in SepMate50 tubes (StemCell Technologies, 85450), followed by two washes in PBS/2%FBS/EDTA ...
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bioRxiv - Immunology 2023Quote: ... PBMC were isolated from blood collected in ethylenediaminetetraacetic acid-anticoagulated (EDTA) tubes by lymphocyte separation medium density gradients (Stemcell technologies, cat# 07801) and resuspended in PRMI 1640 medium supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were digested into clumps with 0.5 mM EDTA for normal culture or digested into single cells with Accutase for passaging (STEMCELL Technologies, Cat# 07920) and cultured with medium containing 10 μM ROCK inhibitor Y-27632 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Akt-transduced BMECs were selected for seven days in serum- and cytokine-free StemSpan SFEM (StemCell Technologies, Inc. 09650) media ...
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bioRxiv - Biochemistry 2019Quote: ... Embryoid bodies (EBs) were generated from feeder-free iPSCs by incubating colonies with Collagenase Type IV (7909, StemCell Technologies) for 10 min ...
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bioRxiv - Biophysics 2019Quote: ... Cells were washed and resuspended in phenol red-free Neurobasal Medium (Lifetech) supplemented with L-glutamine (Stem Cell Technologies) and SM1 (NeuroCult ...
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bioRxiv - Cancer Biology 2019Quote: Neurosphere (sphere) cultures were established in serum-free media as described (9) (NeuroCult™ Media, Stem Cell Technologies, #05700) supplemented with human PDGF-AA (20 ng/mL ...
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bioRxiv - Neuroscience 2020Quote: ... LIN28 and shRNA against p53 [28] in feeder- and serum-free conditions using TeSR-E7 medium (Stem Cell Technologies). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... mESCs were cultured in feeder free conditions with 5% CO2 at 37°C on 0.1% gelatin (StemCell Technologies, 7903) coated plates in 2i medium supplemented with 2% mES certified FBS (Gibco 16141061) ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... The cells were cultured in feeder-free conditions in 10 cm culture dishes in TeSR-E8 (Stem Cell Tech) medium on SyntheMAX (2µg/cm2 ...
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bioRxiv - Pathology 2019Quote: ... All iPSC lines were cultured on LDEV-free hESC-qualified Geltrex-coated tissue culture dishes in mTeSR1 (Stemcell Technologies) medium supplemented with 1% penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... We grew cells in a 5% CO2 incubator in a serum-free NeuroCult NS-A basal medium (STEMCELL Technologies) supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2) at a cell concentration of 5 × 105/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) supplemented with 100 ng/mL of human recombinant SCF ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were plated immediately into pre-warmed antibiotic-free HSPC media (StemSpan SFEM II; Stemcell Technologies; cat. no., 09605_C) supplemented with SCF (100 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Primary CD4+ T cells were purified from the blood of healthy donors by negative selection using the Human CD4+ T Cell Enrichment cocktail (StemCell Technologies). These primary cells were cultured in complete RPMI 1640 medium.
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were isolated from peripheral blood of donor 2395 via negative selection using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood NK cells were isolated from blood collars using a RosetteSep™ human NK cell enrichment cocktail (STEMCELL Technologies). NK cells were then co-cultured for 4 h with confluent monolayers of SH-SY5Y parental ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation of the cells with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2022Quote: T cells were isolated from human donor whole blood samples by negative selection using the RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies). Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with a cocktail of lineage specific antibodies (CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and GlyA; Stemcell Technologies) for negative selection of Lin− cells using an EasySep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were enriched from blood of healthy donors using RosetteSep Human CD4+ T cell enrichment cocktail (Stemcell Technologies, Canada) according to the manufacturer’s instructions followed by Ficoll density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... 100μL of Isolation Cocktail and 100μL of Rapid Spheres (Easy Sep™ Direct Human Total Lymphocytes Isolation Kit: 19655, StemCell Technologies) were added to the remaining whole blood ...
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bioRxiv - Immunology 2022Quote: Monocytes isolated from BCs were obtained first by enrichment of the BC with 15µL of RosetteSep human monocyte enrichment cocktail (Stemcell Technologies, Sweden) per mL of BC ...
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Molecular Biology 2023Quote: ... Peripheral Blood Mononuclear Cells (PMBCs) were isolated from the buffy coat of whole blood (20 mL) using RosetteSep human enrichment cocktail (STEMCELL Technologies) and density gradient centrifugation as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Bone marrow aspirates were filtered through a cell strainer and mixed with a RosetteSep human MM cell enrichment cocktail (negative selection, STEMCELL Technologies). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... was added into the dissociated CO/PCCO single cells suspension together with 1 µL Biotin selection cocktail (Annexin V, STEMCELL Technology), gently mixed and incubated at RT for 3 min ...
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bioRxiv - Neuroscience 2020Quote: ... and expanded using ReLeSR and hESC medium plus ROCK inhibitor (Y-27632, Stem Cell Technologies). The iPSC lines were generated in this way from six unrelated people with DS (QM-DS1 ...
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bioRxiv - Neuroscience 2020Quote: ... and seeded using mTSER media supplemented with 10μM Rock inhibitor (Y-27632, StemCell Technologies, #72304). Media was replaced every day.
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bioRxiv - Molecular Biology 2021Quote: ... cells were cultivated in StemFlex™ cell culture medium supplemented with ROCK inhibitor (STEMCELL Technologies) and HDR enhancer (Integrated DNA Technologies).
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bioRxiv - Genomics 2021Quote: ... ESC/iPSCs were plated in iPS-Brew with 10 μM ROCK inhibitor (Stem Cell Technologies) onto Matrigel-coated (1 mg ml-1 ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL Rho-kinase inhibitor (RockI; Y-27632 STEMCELL Technologies, cat. 72304). To prepare the differentiation experiments ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 μM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 50 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Stem Cell Technologies). The cell suspension was distributed into ultra-low attachment 96-well plates (Corning) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then treated with Wnt pathway inhibitor IWP-4 (5 µM) (Stemcell Technologies, #72552) for 48 hours followed by CM(- ...
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bioRxiv - Immunology 2023Quote: ... mTeSR1 was supplemented with the ROCK inhibitor Y-27632 (STEMCELL Technologies, 72308, 5 μM final).
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bioRxiv - Immunology 2023Quote: ... mTeSR1 was supplemented with the ROCK inhibitor Y-27632 (STEMCELL Technologies, 72308, 7.5 μM final). Cells were partially dissociated to small aggregates/clumps for passaging with 1× TrypLE Express (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were split using Accutase (Merk) and cells were seeded with Rock Inhibitor (STEMCELL Technologies) (1:1000 ...