Labshake search
Citations for Stemcell Technologies :
251 - 300 of 574 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... CD34+ cells (1 × 105/mL) were suspended in serum-free expansion medium II (Stem Cell Technologies) with 50 ng/mL stem cell factor (PeproTech ...
-
bioRxiv - Developmental Biology 2021Quote: ... feeder-free maintenance medium (mTeSR for human ES and iPS cells, Stem Cell Technologies, cat#85850) as previously described (Low et al. ...
-
bioRxiv - Bioengineering 2021Quote: CD34+ leukemic blasts were cultured in StemSpan™ SFEM II serum-free medium (STEMCELL Technologies, 09605) supplemented with the StemSpan™ CD34+ Expansion supplement (STEMCELL Technologies ...
-
bioRxiv - Developmental Biology 2021Quote: ... H7 (WiCell) pluripotent stem cells were maintained in feeder-free conditions using mTeSR1 media (StemCell Technologies) + 1% penicillin/streptomycin (Thermo Fisher) ...
-
bioRxiv - Genomics 2019Quote: ... H1 human ESCs (WA01, WiCell Research Institute) were maintained in feeder-free mTesR1 medium (Stemcell Technologies). hESCs (passage 26 ...
-
bioRxiv - Cell Biology 2021Quote: ... hiPSCs were then incubated in imaging media of phenol red-free mTeSR1 media (Stem Cell Technologies) with 1% penicillin streptomycin (Thermo Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... The iPSC lines were cultured on feeder-free Matrigel-coated plates in mTeSR media (StemCell Technologies) before differentiation into forebrain cerebral organoids.
-
bioRxiv - Genomics 2023Quote: ... iPSCs were maintained in feeder-free conditions in mTESR1 (85850, Stem Cell Technology, Vancouver, BC, Canada) with 1% Penicillin/Streptomycin (30-002-Cl ...
-
bioRxiv - Bioengineering 2023Quote: ... the cells were dissociated using Animal Component-Free (ACF) enzymatic dissociation solution (Stemcell Technologies, Cologne, Germany). Then ...
-
bioRxiv - Developmental Biology 2024Quote: ... hESCs were cultured under feeder-free conditions in mTeSR Plus media (Stem Cell Technologies, 100-0276) on plates coated with Matrigel (Corning ...
-
bioRxiv - Neuroscience 2024Quote: ... GBM cells were grown in serum-free Neurocult NS-A Basal medium (Stem Cell Technologies #05750) that is supplemented with 2mM L-Glutamine (Wisent #609—65-EL) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 250 cells were plated in triplicate in cytokine-free methylcellulose media (Methocult GM M3234, StemCell Technologies) supplemented with 20 ng/mL IL-3 and 20 ng/mL SCF (R&D Systems) ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were cultured in StemSpan™ Serum-Free Expansion Medium version II (SFEM II; STEMCELL Technologies) containing 50 ng/mL each of recombinant human stem cell factor (SCF ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were sorted directly into 200 μl Stem Span Serum-Free Expansion Medium (SFEM, StemCell Technologies), supplemented with 100 units/ml penicillin ...
-
bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
-
bioRxiv - Immunology 2022Quote: ... CD4+ T cells were enriched by Lymphoprep and RosetteSep Human CD4+ T Cell Enrichment Cocktail (both STEMCELL Technologies). CD4-enriched cells were then CD25-enriched using CD25 MicroBeads II (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2023Quote: ... The minced tissue was then placed in an enzyme cocktail consisting of 1U/ml dispase (Stemcell Technologies, 07913), 2,000U/ml collagenase I (Worthington ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies, 15023 and 15022) and EasySep Human CD4 T Cell Iso Kit (Stemcell Technologies ...
-
bioRxiv - Genomics 2021Quote: ... cells were centrifuged (350 x g for 5 minutes) and resuspended in 5 mL of prewarmed 0.25% trypsin/EDTA (#07901, Stemcell Technologies) at 37°C for 5 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.1mM EDTA-2Na).Lineage negative (Lin−) cells were enriched using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell technologies) and stained with fluorescent antibodies ...
-
bioRxiv - Cancer Biology 2020Quote: ... and the cells then dissociated in 2.5 mg/ml trypsin with 1 mM EDTA and 5 mg/ml dispase (STEMCELL Technologies) with 100 μg/ml DNaseI (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells for feeder free systems were passaged with a non-enzymatic disassociation solution ReLeSR (Stem Cell Technologies) according to manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: Flow sorted cells were seeded in serum-free methylcellulose-based medium with cytokines (MethoCult SFH4436, Stemcell technologies). 5 μM imiquimod or DMSO were added to the methocult at the time of seeding in a separate location within the media prior to vortexing ...
-
bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF, STEMCELL Technologies, 78139), 0.2% Chemically Defined Lipid Concentrate (Thermofisher ...
-
bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF, STEMCELL Technologies, 78139), 0.2% Chemically Defined Lipid Concentrate (Thermofisher ...
-
bioRxiv - Neuroscience 2021Quote: ... under a feeder-free condition and were passaged once per week with ReLeSR medium (STEM CELL Technologies). All hPSC studies were approved by the Embryonic Stem Cell Research Oversight committee at Rutgers University.
-
bioRxiv - Neuroscience 2021Quote: ... UK) at passage 32 were maintained under feeder-free conditions on vitronectin (STEMCELL Technologies, British Columbia, Canada)-coated 6-well plates in E8 medium (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... G797 cells were maintained in serum-free NeuroCult™ NS-A Basal Medium (STEMCELL Technologies Canada Inc) supplemented with N2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The hPSC lines were cultured in serum-free defined conditioned media mTeSR or mTeSR plus (STEMCELL Technologies) on hES-certified matrigel (Corning cat#354277) ...
-
bioRxiv - Systems Biology 2021Quote: H1 male hESC cell line was maintained under feeder-free conditions in mTeSR1 medium (Stem Cell Technologies) supplemented with 1% antibiotic-antimycotic (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The isolated monocytes were counted and resuspended in growth medium (Stemcell Technologies, DC differentiation Medium, serum-free) supplemented with GM-CSF (1000 U/mL ...
-
bioRxiv - Immunology 2023Quote: ... and stem cells were passaged weekly using enzyme free dissociation reagent ReLeSR™ (Stem Cell Tech #05872). Cultures were maintained in an undifferentiated state ...
-
bioRxiv - Genetics 2023Quote: ... were transitioned from MEF-dependent culture to feeder-free culture using mTeSR® medium (Stem Cell Technologies) and 6-well plates coated with hESC-qualified Matrigel® (Corning ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 10-M RHO/ROCK pathway inhibitor Y-276932 (STEMCELL Technologies Cat# 72304) were used to dissociate iPSCs to single cells during passaging ...
-
bioRxiv - Genomics 2020Quote: ... cells were treated with 10 μM ROCK Inhibitor (Y-27632) (Stem Cell Technologies) in Essential 8 (E8 ...
-
bioRxiv - Bioengineering 2021Quote: ... with or without 10 ng/mL ROCK inhibitor Y-27632 (STEMCELL Technologies, 72302). The condition which promoted the best viability and propagation was then applied to primary SSC cultures for 3 passages at 37°C and 5% CO2 and 2 freeze-thaw cycles ...
-
bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were treated with 10 μM ROCK inhibitor Y-27632 (STEMCELL Technologies). The cells were washed using Dulbecco’s Phosphate-Buffered Saline (DPBS ...
-
bioRxiv - Bioengineering 2021Quote: ... media was additionally supplemented with the ROCK inhibitor Y-27632 (Stemcell Technologies, 72308) at 7.5uM and 5uM concentrations respectively ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with 10 μM ROCK inhibitor Y-27632 (RI; 05990, STEMCELL Technologies, Canada) for the first 24 h after thawing or passaging ...
-
bioRxiv - Immunology 2023Quote: ... moDCs were treated with 10 μM RALDH inhibitor diethylaminobenzaldehyde (DEAB) (Stem Cell Technologies), 10 μM of PPARγ inhibitor (GW9662) ...
-
bioRxiv - Developmental Biology 2023Quote: ... immersed in mTeSR1 media with 10 μM ROCK inhibitor (Y-27632, STEMCELL Technologies) to prevent dissociation- induced apoptosis and transferred to an incubator at 37°C and 5% CO2 ...
-
bioRxiv - Neuroscience 2023Quote: ... and supplemented with WNT pathway inhibitor IWP-2 (2.5 μM; STEMCELL Technologies, 72124) and recombinant Human/Murine/Rat Activin A (50 ng ml−1 ...
-
bioRxiv - Cell Biology 2023Quote: ... and resuspended in media supplemented with 10$M ROCK inhibitor Y27632 (STEMCELL Technologies). Cells were then seeded onto Geltrex-coated plates (12-well ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... media supplemented with 10μM of the ROCK inhibitor Y27632 (STEMCELL Technologies, cat # 72302). 24h later ...
-
bioRxiv - Genomics 2024Quote: ... Medium was supplemented with 10uM ROCK inhibitor (ROCKi) (Y-27632, Stemcell Tech, 72304) for 16-48 hours when cells were passaged with Accutase (StemCell Tech ...
-
bioRxiv - Cell Biology 2022Quote: ... CD4+ cells were isolated by negative selection (RosetteSepTM Human CD4+ T cell Enrichment Cocktail, STEMCELL technologies, Cambridge, UK; #15023) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using EasySep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5x10 6 cells/mL left overnight for recovery ...
-
bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies) or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... centrifugation were enriched for CD34+ cells using the RosetteSep™ Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies) as per manufacturer’s instructions.