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Citations for Stemcell Technologies :
401 - 450 of 4776 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured for 2 days in StemSpan (Stem Cell Technologies, Cat. 09655) supplemented with TPO (100ng/mL) ...
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bioRxiv - Microbiology 2024Quote: ... M2-10B4 and S1/S1 stromal cells were obtained from Stem Cell Technologies and maintained in DMEM with 10% FBS and penicillin ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were maintained in mTeSR media (Stem Cell Technologies, catalog no. 85850), which was replenished daily ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were dissociated into a single-cell solution with Accutase (STEMCELL Technologies), and 9,000 live cells were seeded in each well of U-bottom Ultralow attachment 96-well plates (Corning ...
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High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... iPS11 cells were dissociated using Gentle Cell Dissociation Reagent (STEMCELL Technologies, 100-1077) according to manufacturer’s instructions and counted using a hemocytometer ...
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bioRxiv - Neuroscience 2024Quote: ... PSCs were dissociated to single cells using Gentle Cell Dissociation Reagent (StemCell Technologies) and plated in AggreWell™800 culture plates (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were incubated in Accutase cell detachment medium (Stem Cell Technologies, 07922, 07920) at 37 °C for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... For cryopreservation cells were resuspended in CryoStor ® (Stem Cell Technologies, 100-1061) and frozen at - 80 °C.
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bioRxiv - Immunology 2024Quote: ... Live cells were enriched using the EasySep Dead Cell Removal kit (Stemcell Technologies). For scRNAseq of pre-enriched monocytes ...
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bioRxiv - Bioengineering 2024Quote: ... cell aggregates were gently detached using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). To prevent spontaneous differentiation ...
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bioRxiv - Cell Biology 2024Quote: 500 freshly sorted cells were seeded in MethoCult GF M3434 (Stem Cell Technologies) and colonies were scored after 10 days ...
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bioRxiv - Cancer Biology 2024Quote: ... CD8+ T cells were isolated from human peripheral blood mononuclear cells (STEMCELL Technologies) using the EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2024Quote: HUES8 human embryonic stem cells (hESCs) were cultured mTeSR1 (Stem Cell Technologies, 05850) media and incubated at 37 °C with 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... and iEndo-mKate2 PGP1 cells were dissociated from 70%-80% confluency by incubating cells in Gentle Cell Dissociation Reagent (STEMCELL Technologies, #07174) for 12 min at 37°C ...
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bioRxiv - Immunology 2021Quote: NK cells were purified from uninfected spleens of WT and CypD-/- mice using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies) according to the supplier’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6.OT-I.Ubiquitin-GFP CD8+ T cells were enriched from single-cell suspensions of lymph nodes and spleens using mouse CD8-negative selection kits (Stem Cell Technologies). Enriched CD8 T cells (5×106 ...
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were fused with SP2/0 Ag14 myeloma cells (Kohler and Milstein, 1975) and hybrid cells were selected by growth in methylcellulose-containing HAT medium (Stem Cell Technologies). Approximately 600 clones were isolated and transferred to liquid HT medium in 96-well plates ...
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bioRxiv - Cell Biology 2021Quote: ... DKC1 A353V (AAVS1-EGFP) iPS cells and DKC1 A353V mutation corrected (AAVS1-EGFP) iPS cells) were maintained on vitronectin (Stem Cell Tech.) coated plates using StemMACS™ iPS-Brew XF medium (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... Passage one cells were seeded into T75 cell culture flasks (250,000 cells/flask) and expanded in PneumaCult ExPlus medium (StemCell Technologies, Vancouver, Canada) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... cells were sorted for single cells for transfected (GFP+) cells and plated into prewarmed (37C) StemFlex media supplemented with 1X CloneR (Stem Cell Technologies) onto Vitronectin XF (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... Naïve human CD4+ T cells were isolated using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
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bioRxiv - Immunology 2020Quote: ... and CD8+ T cells were negatively isolated using the EasySep™ Mouse CD8+ T cell Isolation Kit (Stem Cell Technologies, Grenoble, France) or MojoSort™ Isolation Kits (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Purity and viability of isolated T cells was assessed using flow cytometry prior to cryopreservation of isolated T cells in CryoStore CS10 cryopreservation media (Stem Cell Technologies) at a density of 1-1.5×107 cells per ml.
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... via Ficoll-Paque (Cytavia) gradient selection for peripheral blood mono-nuclear cells followed by human CD8+ T cell negative magnetic selection (Stem Cell Technologies). Primary CD8+ T cells were thawed in supplemented RPMI 1640 2-12 hours prior to the start of experiments.
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were then seeded into 96-well round bottom plates (cell density at 100,000 (100k) cells/100 μL) and prestimulated in StemSpanTM SFEM (Stem Cell Technologies #09650) supplemented with StemSpan™ CC100 (Stem Cell Technologies #02690) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were plated for single cell clones by plating 1000 cells per 10cm2 dish on Synthemax with 1x CloneR (Stemcell technologies #05888), and single cell clones were genotyped by PCR for correct integration before sequencing both alleles from positive clones.
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC-derived DA neurons were plated on a 48-well Classic MEA plate (Axion BioSystems) at a cell density of 1.2×105 cells per well and maintained in culture using Complete BrainPhys™ (Stem Cell Technologies). Culture Medium prepared as directed in the iCell DopaNeurons MEA Application Protocol (https://www.fujifilmcdi.com/) ...
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bioRxiv - Immunology 2023Quote: ... filtered through 40 µm cell strainers and T cells enriched by negative selection using an EasySep™ pan-T cell enrichment kit (Stemcell Technologies) as per manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium containing hematopoietic cells and trypsinized cells were collected for FACS analysis and CFU-C assay in Methylcellulose M3434 (Stem Cell Technologies). After 10 days of culture at 37°C in 5% CO2 ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6low and CCR6high memory subgroups and cultured at 1 × 106 cells/ml in 24 well plates in ImmunoCult XF T cell expansion medium (Stem Cell Technologies) supplemented with 10% FBS and IL-2 (200 units/ml) ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Cell Biology 2023Quote: ... Accutase was quenched and single cells were seeded for differentiation in suspension at a density of 1×106 cells/mL in complete mTeSR media (Stem Cell Technologies) supplemented with 10ng/mL FGF2 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells and isolated CD4+ T cells were activated using Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies, 10970) per manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... apoptotic and dead cells were removed by immunomagnetic cell separation using the Annexin Dead Cell Removal Kit (StemCell Technologies, cat. no. 17899) and EasySep Magnet (StemCell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Immunology 2024Quote: ... B-cells were then purified from the other harvested PBMCs by negative selection using a Human B-cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Brachial and Axillary) and CD4+ve T cells were isolated using EasySep™ Mouse CD4+ T Cell Isolation Kit (STEM CELL TECH). Protein lysate of snap-freeze tissue in liquid nitrogen was prepared by homogenizing 5mg tissue in 500μl ice-cold RIPA lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Colony formation assays were established using primary AML BM cells cultured at 1×105 cells/ml in MethoCult-H4434 (Stem Cell Technologies) in the presence of vehicle or drug ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples for SLAM population analysis and cell sorting were first subjected to lineage-negative population enrichment using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...