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Citations for Stemcell Technologies :
651 - 700 of 4776 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The cell pellet was resuspended at 10,000 cells/µl in BrainPhys (Stemcell Technologies Australia, Melbourne, Australia) neural maintenance media with Rho Kinase Inhibitor IV (Stemcell Technologies Australia ...
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bioRxiv - Biophysics 2020Quote: ... cell monolayers were maintained for 20-30 days in STEMDiff cardiomyocte maintenance media (STEM CELL Technologies), on EStim classic 48 well MEA plates (Axion Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were cultured in Mammocult media supplemented with 4 μg/ml Heparin (Stem Cell Technology), 0.5 μg/ml hydrocortisone and 10% Mammocult Proliferation Supplement (Stem Cell Technology) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were adjusted to 1×105 cells/ml final concentration in H4230 methylcellulose medium (STEMCELL Technologies) supplemented with 10% 5637-CM as a stimulant and then plated in 35-mm petri dishes in duplicate and allowed to grow for 7 days in a humidified CO2 incubator (5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: Cells were dissociated from the well using Gentle Cell Dissociation reagent (StemCell Technologies, Cat#: 100-0485). Total proteins were extracted by adding 1ml of cold 10% TCA to the cell pellets ...
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bioRxiv - Cell Biology 2021Quote: ... Cell colonies were separately dissociated and transferred into 6-well plates using ReLeSR (Stem Cell Technologies). Differentiated cells were removed by using a pipette tip to minimize spontaneous differentiation ...
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bioRxiv - Microbiology 2020Quote: ... CD45− cells were magnetically sorted using Ter119/CD45-depletion kit (#19771; Stem Cell Technologies, Vancouver, BC). Next ...
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bioRxiv - Microbiology 2021Quote: ... and stem cells were passaged using enzyme free dissociation reagent ReLeSR™ (Stem Cell Tech#05872). Cultures were maintained in an undifferentiated state ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were cultured in ImmunoCult cell culture media (Stemcell Technologies, Cat no. 10981) supplemented with 35 ng/ml human IL-2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... bulk CD4+ T cells were isolated using the EasySep CD4+ T cell Enrichment Kit (StemCell Technologies). Isolated CD4+ T cells were maintained in RPMI medium supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin (R10 medium) ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were re-suspended in 500μl and counted in 500μl ammonium chloride (Stem Cell Technologies) using the Vicell XR (Beckman Coulter) ...
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bioRxiv - Developmental Biology 2022Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies) with 2 μM ROCK inhibitor (Thiazovivin/TZV ...
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bioRxiv - Cancer Biology 2022Quote: Bone marrow cells were subjected to RBC lysis using Ammonium Chloride lysis buffer (Stem Cell Technologies), then incubated with anti-mouse direct lineage depletion cocktail (Miltenyi ...
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bioRxiv - Immunology 2022Quote: NK cells from spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Sorted cells were plated in triplicate in semi-solid methylcellulose medium (Methocult M3434; Stem Cell Technologies) at 1000 cells/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was changed every day and cells were passaged using gentle cell dissociation reagent (Stemcell Technologies) or singularised using accutase (Stem Cell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched using an EasySep magnetic negative selection kit (Stem Cell Technologies #19853) as per the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit, STEMCELL Technologies) and mRNA was extracted and purified (PureLink RNA Mini Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... RBC-lysed 50,000 whole bone marrow cells were seeded in 1.5mL MethoCult M3434 (Stem Cell Technologies) with no additional supplemental cytokines in triplicate on 6 well plates and scored on day 8 ...
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bioRxiv - Bioengineering 2020Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then treated with 2ml of 0.25% Trypsin/EDTA solution (Stem Cell Technologies, cat #07901) and 2ml of 5mg/mL dispase (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: Unencapsulated 50 cell aggregates were created by dissociation of human iPSCs with Accutase (STEM CELL Technologies) and reseeding in mTeSR™-1 medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... Primary NK cells were separated from donor PBMCs via magnetic bead negative selection (Stem Cell technologies). 96well ELISA plates (Nunc Maxisorp ...
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bioRxiv - Immunology 2021Quote: ... PE positive cells were enriched using an EasyEights™ magnet (Stem Cell Technologies, Cat no. 18103). Three rounds of separation were performed in total and cells were washed in complete media prior to maintenance in culture with complete RPMI + IL-2 ...
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bioRxiv - Immunology 2021Quote: ... EasySep Mouse Naive CD4+ T cell and CD8+ T cell Isolation Kit (StemCell Technologies, Vancouer, Canada) were purchased.
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bioRxiv - Cell Biology 2021Quote: ... CD34+ cells (1 × 105/mL) were suspended in serum-free expansion medium II (Stem Cell Technologies) with 50 ng/mL stem cell factor (PeproTech ...
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bioRxiv - Developmental Biology 2021Quote: ... feeder-free maintenance medium (mTeSR for human ES and iPS cells, Stem Cell Technologies, cat#85850) as previously described (Low et al. ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSCs were detached from the Vitronectin XF substrate using Gentle Cell Dissociation Reagent (Stem Cell Technologies), centrifuged ...
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells or CD4+ T cells were isolated via Lymphoprep and RosetteSep (both STEMCELL Technologies). For Treg isolation ...
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bioRxiv - Immunology 2020Quote: ... T-cells were obtained from PBMCs using the EasySep Human T-cell Isolation Kit (Stemcell Technologies). MLRs were set up in 96 well plates ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated with EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies, 17952).
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bioRxiv - Immunology 2021Quote: ... cells were washed in PBS and incubated in PBS + 50 nM dasatinib (Stem Cell Technologies, Canada) + DNAse at 37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and T cells isolated using the EasySep Human T Cell Isolation kit (Stemcell Technologies, #17951). Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells from healthy donors were purified from leukopaks purchased from Stem Cell Technologies using Ficoll-Paque PLUS (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: CD3ε+ T cells were isolated using negative selection Human T cell Isolation Kit (StemCell Technology, #17951). Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... single cell suspensions were prepared by treatment of hES cells with accutase (#07920, StemCell Technologies Inc.) and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307 ...
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bioRxiv - Immunology 2022Quote: ... NK cells isolated from buffy coats using an EasySep human NK cell enrichment kit (StemCell Technologies) were added to the mixed target population at a 1:1 effector:target ratio ...
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bioRxiv - Physiology 2023Quote: ... Cells were washed with 1x PBS and plated in methocullulose media (Methocult M3434, Stem Cell Technologies). Cells were plated (4 × 105 cells per 35 mm-tissue culture dish ...
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bioRxiv - Neuroscience 2024Quote: ... Cell clusters floating in suspension were dissociated into single-cells with Accutase (Stemcell technologies, Cambridge, MA). The single cell population was resuspended in their respective NDM/GDM media supplemented with RI (StemGent ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
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bioRxiv - Neuroscience 2024Quote: ... GBM cells were grown in serum-free Neurocult NS-A Basal medium (Stem Cell Technologies #05750) that is supplemented with 2mM L-Glutamine (Wisent #609—65-EL) ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were isolated using the EasySep Mouse CD8+ T cell isolation kit (StemCell Technologies) and resuspended at a concentration of 106 cells/mL in complete RPMI supplemented with 1X sodium pyruvate (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from PMBCs using a Nonhuman Primate T-Cell Isolation Kit (STEMCELL Technologies), activated with a nonhuman primate T-Cell Activation/Expansion Kit (Miltenyi Biotec) ...
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bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Primary T cells were isolated from PBMCs using EasySep Human T Cells Isolation Kits (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Dendritic cells were isolated with the EasySepTM Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies). T Cells were isolated with the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... NK cells were enriched from PBMCs by EasySep™ Human NK Cell Isolation Kit (StemCell Technologies) and cultured at 37 °C in 5% CO2 in RPMI 1640 (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell clusters were released from Matrigel by incubation in dispase 5U/mL (Stem Cell Technologies 07913) for up to 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD34+ cells were purified from PBMNCs by the magnetic separation method (EasySep, Stem Cell Technologies) and were cryopreserved ...