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Citations for Stemcell Technologies :
601 - 650 of 4776 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were isolated using EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) by immunomagnetic negative selection according to manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were isolated using EasySepTM Direct Human T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich ...
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bioRxiv - Physiology 2022Quote: ... Selected cells (1 × 105 cells/ml) were expanded in StemSpan H3000 media (StemCell Technologies Inc) supplemented with StemSpam CC100 (StemCell Technologies Inc ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated by EasySep™ Human T Cell Isolation Kits (STEMCELL Technologies, 17951). Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... NK cells were isolated by EasySep™ Human NK Cell Isolation Kits (STEMCELL Technologies, 17955) and activated by 100 Gy irradiated K562 Clone 9 feeder cells ...
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bioRxiv - Cell Biology 2023Quote: ... 2.25·106 cells/well were seeded onto AggreWell TM800 24-well plates (Stem Cell Technologies) in 2mL SCDM (described above) ...
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bioRxiv - Immunology 2022Quote: ... splenic NK cells were isolated using the EasySep mouse NK cell isolation kit (Stemcell Technologies) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... cells were pre-isolated with EasyStep Mouse CD4+ T cell isolation Kit (STEMCELL Technologies Inc) and stained with the fluorochrome-conjugated mAbs targeting CD4 ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were activated for 72 hours with ImmunoCult T cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained in mTeSR+ medium (Stem Cell Technologies #05825) on tissue culture-treated plates coated with Matrigel (Corning #354277) ...
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bioRxiv - Cancer Biology 2023Quote: Primary cells are resuspended in a 3:4 solution of Mammocult medium (Stem Cell Technology) and Matrigel (Corning)17,26,29 ...
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bioRxiv - Immunology 2023Quote: ... CD14+ Mo were FACS sorted from frozen human bone marrow mononuclear cells (Stem Cell Technologies) on a BD FACS Melody Cell Sorted (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: T cells were enriched using the Easy Sep Human T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies, cat #07930) at −80 °C until use (50,000 cells per tube) ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Stimulation was done using ImmunoCult human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies) in non-polarizing conditions using anti-IL-4 (5 μg/ml) ...
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bioRxiv - Microbiology 2023Quote: ... NHBE cells were maintained in filter-sterilized PneumaCult-Ex-PLUS Medium (Stem Cell Technologies #05040) supplemented with 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were dissociated into single cells using Gentle Cell Dissociation Reagent (STEMCELL Technologies, cat # 07174) for 8 minutes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... these cells were used in 3 mL of methylcellulose medium (H4435; Stem Cell Technology, Canada) plated in a 35-mm culture dish (Grainger) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were harvested and dissociated into single cells by incubation with Accutase (STEMCELL Technologies) for 10 min at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... during their expansion phase the cells were cultured in StemSpan H3000 medium (Stem Cell Technologies) supplemented with 100 ng/mL stem- cell factor (SCF ...
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bioRxiv - Developmental Biology 2023Quote: Expanding PP cells were routinely frozen at later passages using mFreSR (Stem Cell technologies, 05854), supplemented with 20 μM ROCKi ...
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bioRxiv - Cell Biology 2023Quote: Thawed CD34+ cells were cultured in StemSpan SFEM II serum-free medium (Stem cell technologies) supplemented with recombinant cytokines (Peprotech Asia) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured at 37°C with 5% CO2 in StemSpan (Stem Cell Technology #09650) supplemented with TPO (0,125ng/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... induced the stem cells into neural progenitor cells using Stemdiff SMADi induction media (STEMCELL Technologies) within a monolayer over the course of 21-30 days on 10 μg/mL poly-L-ornithine (P3655 Millipore Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were expanded using the ImmunoCultTM NK Cell Expansion Kit (STEMCELL Technologies 100-0711), per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... cells grown to a density of 80% were digested with Accutase (Stem Cell Technologies, 07920) for 7 min ...
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bioRxiv - Molecular Biology 2024Quote: ... cell culture plates and differentiated using the STEMdiff Forebrain Neuron Differentiation Kit (Stem Cell Technologies) followed by the STEMdiff Forebrain Neuron Maturation Kit (Stem Cells Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Cells were expanded with ImmunoCult™ human CD2/CD3/CD28 T cell activator (Stemcell Technologies). Four types of cultures were established ...
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bioRxiv - Molecular Biology 2024Quote: ... HUDEP-2 cells were maintained and expanded in serum-free expansion medium (Stem Cell Technologies) supplemented with human Stem Cell Factor (SCF ...
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bioRxiv - Genomics 2024Quote: ... These cells were processed using the EasySep Dead Cell Removal Annexin V Kit (StemCell Technologies), which increased viability to ∼70% and cells were maintained at 1e6 cells per ml with puromycin ...
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bioRxiv - Immunology 2024Quote: ... 2e6 T cells enriched from PBMCs via EasySep Human T Cell Isolation Kit (STEMCELL Technologies) were seeded in the top or bottom chambers of the Transwell culture ...
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bioRxiv - Bioengineering 2024Quote: ... stem cells at 60-80% confluency were dissociated into single cells using Accutase (STEMCELL Technologies), followed by centrifugation and resuspension at a density of 0.3 million cells per milliliter in mTeSR1 supplemented with 10µM Y-27632 (a ROCK inhibitor) ...
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bioRxiv - Bioengineering 2024Quote: ... iPS cells were propagated as colonies using ReleSR (Stem Cell Technologies cat. no. 100-0483) upon reaching 70% confluency ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell suspension was added to 1.5 mL methylcellulose media (MethoCult GF #M3434, Stem Cell Technologies) supplemented with 1X antibiotic-antimycotic (#15240-062 ...
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bioRxiv - Neuroscience 2024Quote: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies. NPCs were cultured on matrigel-coated plates (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... Primary T cells were isolated using the EasySepTM human T cell isolation kit (Stemcell technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were subsequently washed using HBSS with 10 mM HEPES (Stem Cell Technologies, 37150) and incubated for 20 minutes to allow for de-esterification of the AM esters ...
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bioRxiv - Developmental Biology 2024Quote: ... 6 well cell-culture treated plates were coated with 0.1% gelatin (Stem Cell Technologies, 07903) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Immunology 2024Quote: T cells were extracted from OT-II mouse spleens and magnetically sorted using a T-cell extraction kit from Stem Cell Technologies (catalog number 19851). Cells were incubated in culture media (RPMI+ 10% HIFBS + Pen/Strep + 1 mM HEPES + 50 μM beta mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... Anti-CD3/CD28 antibody-coated beads (ImmunoCult) were purchased from STEMCELL technologies (Vancouver, CA).
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bioRxiv - Neuroscience 2021Quote: ... Isotype control was stained using control antibody (STEMCELL Technologies, 60070AD.1, 0.2 ug/ul) at 1:378 dilution for 10 min at 4 °C protected from light ...
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bioRxiv - Bioengineering 2023Quote: ... The following primary antibodies were used: beta-tubulin III (STEMCELL Technologies 60052; 1:1000), GFAP (Dako GA524 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated with Gentle Cell Dissociation Reagent (STEMCELL Technologies 07174 and passaged 1:4 (C1) or 1:6 (C2 ...
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bioRxiv - Developmental Biology 2021Quote: ... and purified for live cells using the EasySep Live/Dead magnetic selection kit (Stem Cell Technologies). Suspensions were passed through a 40-μm mesh filter to obtain single cells.
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bioRxiv - Genomics 2020Quote: ... Cells were first differentiated into definitive endoderm using the STEMdiff Definitive Endoderm Kit (Stem Cell Technologies) for 72 hours and subsequently dissociated with GCDR and passaged as small clumps into growth factor reduced Matrigel-coated (Corning ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from naïve spleens with the EasySep T cell isolation kit (STEMCELL Technologies). For each mouse 4×106 naïve T cells were injected intravenously via the tail ...
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bioRxiv - Cancer Biology 2020Quote: CD4+ T cells were isolated using the EasySep Mouse CD4+ T-cell isolation kit (Stemcell Technologies). The CD4+ T cells were then added in a 1:1 ratio to cultured CMT167 cells ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...