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Citations for Stemcell Technologies :
351 - 400 of 605 citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... splenocytes were first enriched for OT-II CD4+ T cells using EasySep™ mouse CD4+ T cell isolation kit (StemCell Technologies), incubated with FcR block and stained with the following fluorescence-conjugated antibodies ...
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bioRxiv - Immunology 2020Quote: BMDNs were obtained by crushing long bones the bones in 5% FCS using a mortar and either isolated by negative selection using the EasySep Mouse Neutrophil Enrichment Kit (Stemcell Technologies) or by positive selection using biotinylated antibodies (see supplemental data for more details).
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bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... infection efficiency was determined by GFP expression and infected cells were isolated using RoboSep mouse CD8a positive selection kit II (STEMCELL Technologies Inc. ...
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bioRxiv - Developmental Biology 2020Quote: Methylcellulose-based hematopoietic progenitor assay was routinely used to enumerate haematopoietic progenitors from both mouse (MethoCult™ GF M3434, Stemcell Technologies) and human (MethoCult™ H4434 ...
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bioRxiv - Immunology 2021Quote: ... CD90.2+ cells were pre-sorted from Rag2−/− lung and bone marrow single cell suspensions using an EasySep™ Mouse CD90.2 Positive Selection Kit II (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the mice were irradiated with a dose of 100 cGy per mouse and injected intravenously with 0.2 million of HSCs (purchased from Stemcell Technologies Inc.). After 8–10 weeks ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were either harvested to assess levels of exhaustion marker expression by flow cytometry or DMSO-treated cells were purified using the mouse CD8 T cell isolation kit (StemCell Technologies). Enriched mouse CD8+ T cells were then treated with either DMSO alone or soquelitinib at concentrations ranging from 0 to 10 μM with additional two rounds of stimulation with the OVA peptide ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of naïve (CD44loCD62Lhi) lymph node CD8+ T cells (isolated using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit, Stemcell Technologies) were washed with ice-cold PBS supplemented with 2% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: CD8+ T cells were isolated from the spleens and lymph nodes of OTI mice using the EasySep Mouse CD8+ T Cell Isolation Kit (StemCell Technologies). For direct co-culture experiments ...
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bioRxiv - Neuroscience 2023Quote: Nodose ganglia were dissected from Snca-/- mice and placed in 300 μL ice cold mouse Intesticult media (StemCell Technologies, Cat#06005) containing nerve growth factor-2 (NGF ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from spleens and lymph nodes using the EasySep mouse CD4+ T cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The M210B4 stromal cell line was isolated from normal mouse bone marrow adherent cells50 and maintained in RPMI 1640 medium (STEMCELL Technologies) with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from splenocytes by the EasySep Mouse CD4+ T-cell Isolation Kit (#19852, STEMCELL Technologies, Vancouver, Canada) were used to evaluate the capability to differentiate into different T cell subsets as previously described [18,19,20] ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cell isolation was performed with magnetic separation (EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit, StemCell Technologies).
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: Luminal or luminal progenitor cells were isolated from mouse mammary glands and sorted in 96-well plate filled with 5% FBS EpiCULT full medium (STEMCELL Technologies). 500 luminal cells or 200 luminal progenitors were then transferred to a 48-well plate previously seeded with 10,000 NIH3T3 irradiated feeder cells per well and incubated at 37°C ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from spleens of P14 donor mice by negative selection using the EasySepTM Mouse CD8+ T-cell isolation kit (STEMCELL Technologies). P14 cells were activated in vitro for 24-28 hours with 100U/mL recombinant IL-2 ...
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bioRxiv - Biochemistry 2023Quote: ... neutrophils were purified by an in-house negative selection kit based on a modified mouse-neutrophil positive selection kit EasySep (Stemcell Technologies). The antibodies used for the negative selection were ...
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bioRxiv - Bioengineering 2023Quote: ... splenic T lymphocytes were isolated from Cas9 transgenic mice using negative selection with the EasySep Mouse T Cell Isolation Kit (Stemcell Technologies) and then activated with Dynabeads Mouse T-Activator CD3/CD28 beads (Cat # 11456D ...
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bioRxiv - Immunology 2023Quote: ... Naive B cells were isolated by negative magnetic activated cell sorting using the EasySepTM Mouse B cell Isolation Kit (StemCell Technologies). 30 million B cells were injected i.p ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Cancer Biology 2023Quote: CD8 T cells were isolated from the lymph node of naïve mice with unactivated Confetti cassette using EasySep™ Mouse CD8+ T Cell Isolation Kit (StemCell Technologies) and macrophages were sorted from the tumors via FACS sorting ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from peripheral lymph nodes (inguinal, axillary, brachial and superficial cervical) by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies). CD4+ T cells were stimulated in 96 well flat-bottomed plates coated with 20mg/ml αCD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells from spleens were negatively enriched using an EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853) according to the manufacturer’s instructions (STEMCELL Technologies). Enriched CD8+ T cells (92% - 96% purity ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated from spleens and lymph nodes of P14 Tcf7GFP or OTI Tcf7GFP mice using the EasySepTM Mouse CD8+ T cell Isolation Kit (Stemcell Technologies), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Splenic naïve CD4+ T cells were negatively selected using an EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naïve T cells were resuspended in supplemented RPMI 1640 medium with penicillin (100 U/ml) ...
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bioRxiv - Immunology 2022Quote: ... or GZB-cre Zbtb20-fl/fl CD45.1 OT-I mice (KO) and naive CD8 T cells purified using EasySep Mouse Naive CD8 T Cell Isolation Kits (catalog no. 19858A; STEMCELL Technologies). Fifty thousand naive OT-I cells were retro-orbitally injected into congenic B6 recipient mice ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... and purified CD4+ T cells were obtained by positive selection with the “EasySep Mouse CD4 Positive Selection II” kit (StemCell Technologies). Then ...
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bioRxiv - Developmental Biology 2023Quote: CFU assays were performed according to the Mouse “Colony-Forming Unit” (CFU) Assays Using MethoCult™” GF M3434 protocol (StemCell Technologies) (STEMCELL) ...
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bioRxiv - Cancer Biology 2023Quote: ... transduced mouse bladder urothelial or prostate epithelial organoids were liberated by dissociating Matrigel matrix with Dispase 5 U/ml (STEMCELL Technologies). Organoids were washed with PBS and resuspended in ice cold Matrigel with either 105 EBLM or UGSM and subcutaneously injected into the flanks of six-to eight-week-old male NSG (NOD-SCID-IL2Ry-null ...
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bioRxiv - Immunology 2024Quote: ... OT-I+/- or total CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection using the EasySepTM Mouse CD8+ T cell isolation kit (#19753, StemCell Technologies). For in vitro experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... femur and tibia were flushed and neutrophils (PMN) enrichment was performed with the EasySep mouse neutrophil enrichment kit (Stem Cell Technologies). PMN ROS production upon stimulation with PMA (100 nM ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells (OT-I cells) were isolated by negative selection with the EasySepTM Mouse CD8 T cell isolation kit (Catalog #19853, Stemcell Technologies) following the manufacturers protocol ...
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bioRxiv - Immunology 2024Quote: ... Cells were filtered through a 70 µm nylon strainer and further purified with the EasySepTM mouse neutrophil enrichment kit (StemCell Technologies), following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... CD4+ T were isolated from the spleen and LN of OT-II TCR Tg GFP+ mice using the Easysep Mouse CD4+ T cell isolation kit (Stemcell Technologies) or MojoSort Mouse CD4 Naïve T cell isolation kit (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: Primary mouse NK cells were isolated from spleens of 8-12-week-old wild-type or Prf1-/- mice using the negative selection EasySep mouse NK cell isolation kit (STEMCELL Technologies) or positive selection CD49b (DX5 ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the mouse spleen and injected with mEERL cells with scramble or Marchf8 sgRNA using the CD8+ T-cell Isolation Kit (STEMCELL Technologies). The cells were labeled with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: Splenic B cells from BACtg and wild type littermate control mice were isolated using the EasySep Mouse B Cell Isolation Kit (StemCell Technologies) and rested for 30 minutes and then either left unstimulated or stimulated with the addition of 10 μg/ml goat anti-IgM F(ab’)2 crosslinking antibody (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2024Quote: ... CD8+/CD4+T cells (for OT-I/OT-II mice) were isolated from this suspension using the EasySep Mouse CD8+/CD4+ T Cell Isolation kit (STEMCELL Technologies; Cat#19852 for CD4+T ...
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bioRxiv - Bioengineering 2021Quote: ... Microwells were incubated with Anti-Adherence Rinsing Solution (STEMCELL Technologies) for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... T175 flask were pretreated with anti-adherence solution (STEMCELL Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... and rabbit anti-GFAP (1:500; Stem Cell Technologies 60128) were diluted in 1% blocking solution (normal donkey serum in 0.3% Triton X-100 in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... 500 µl of anti-adherence rinsing solution (STEMCELL Technologies 07010) was added to each well ...
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bioRxiv - Biophysics 2024Quote: ... were treated with anti-adherence rinsing solution (Stem Cell Technologies) to prevent cell adhesion ...
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bioRxiv - Neuroscience 2023Quote: ... pre-treated with Anti-Adherence Rinsing Solution (Stem Cell Technologies) in embryoid body (EB ...
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bioRxiv - Developmental Biology 2022Quote: ... wells were rinsed with anti-adhesion rinsing solution (STEMCELL Technologies), centrifuged at max speed for 5 min ...