Labshake search
Citations for Stemcell Technologies :
301 - 350 of 605 citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: CD8 T cells were purified from spleens by negative selection using EasySep Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
-
bioRxiv - Immunology 2021Quote: ... Untouched CD8+ T cells were magnetically isolated using EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, Grenoble, France) or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend ...
-
bioRxiv - Bioengineering 2020Quote: ... Initial hematopoietic lineage negative enrichment was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA), followed by collection of the Lin- Sca-1+ c-kit+ (LSK ...
-
bioRxiv - Immunology 2022Quote: ... Lm-OVA-specific CD8+ OT-I T cells were isolated from the spleen of infected mice by positive selection using the EasySep mouse CD90.1 positive selection kit (StemCell Technologies) as previously described (2 ...
-
bioRxiv - Immunology 2022Quote: CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies, Canada). Cd4-/- mice were adoptively co-transferred intravenously (i.v. ...
-
bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies). CD4+ T cells (4.0 × 105/well ...
-
bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
-
bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
-
bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
-
bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
-
bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
-
bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
-
bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: F814 and WT bone marrow cells were cultured for 10 days in MesencultTM Expansion Kit for mouse (Stemcell tech, 05513) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... CD8+ T cells were enriched from total splenocytes using the EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) (routinely >90% purity).
-
bioRxiv - Cell Biology 2024Quote: Single cell suspensions of BMMNCs were first lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). Lineage depleted cells were then stained with ...
-
bioRxiv - Immunology 2024Quote: ... The samples were enriched for CD4 T cells using the EasySep™ mouse CD4+ T Cell isolation kit (Stemcell technologies) and the dead cells were removed using EasySep™ Dead Cell Removal (Annexin V ...
-
bioRxiv - Immunology 2024Quote: ... and P14 cells were isolated on 7 dpi using the EasySep Mouse APC Positive Selection Kit II (#17667, STEMcell technologies) with a CD45.2 APC antibody (#17-0454-82 ...
-
bioRxiv - Immunology 2024Quote: Neutrophils were purified from the blood Using the EasySepTM Mouse Neutrophils enrichment kit following the manufacturer’s instructions (Stem Cell Technology).
-
bioRxiv - Immunology 2024Quote: ... Lin-c-Kitint/loFlt3+ progenitors were enriched by using an EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies), biotin-conjugated anti-Thy1.2 (53-2.1) ...
-
bioRxiv - Immunology 2024Quote: ... 1x108 cells/ml were incubated with 20μl/ml EasySepTM mouse Hematopoietic Progenitor Cell Isolation Cocktail (15min, 4°C, StemCell technologies), RapidSpheres (50μl/ml ...
-
bioRxiv - Immunology 2024Quote: CD8 T cells were purified from spleens of CD45.1 infected mice using the EasySep Mouse CD8 T cell isolation kit (Stemcell Technologies). The enriched CD8 cell suspension was then labeled with fluorescent antibodies (CD8 ...
-
bioRxiv - Cancer Biology 2024Quote: ... ES R1 cells were grown on a layer of mouse embryonic fibroblast (MEF) feeder cells (Stem Cell Technologies, CA, US) treated with mitomycin C (for 2 hours at a final concentration of 10 μg/mL ...
-
bioRxiv - Bioengineering 2021Quote: ... an anti-adherence rinsing solution (STEMCELL technologies, Cat# 07010) was used to treat the Aggrewell800™ plate ...
-
bioRxiv - Neuroscience 2020Quote: ... Aggrewells coated with anti-adherence rinsing solution (StemCell Tech), U bottom non-treated polystyrene plates (ThermoFisher #168136 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 500μl of anti-adherence rinsing solution (STEMCELL Technologies 07010) was added to each well ...
-
bioRxiv - Developmental Biology 2023Quote: ... 500ml of anti-adherence rinsing solution (STEMCELL Technologies 07010) was added to each well ...
-
bioRxiv - Developmental Biology 2024Quote: ... pre-treated with Anti-Adherence Rinsing Solution (STEMCELL Technologies). After incubation for 18-24 hours at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
-
bioRxiv - Immunology 2021Quote: ... CECs were isolated from the spleens of anemic mice using the EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
-
Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
-
bioRxiv - Immunology 2021Quote: For sorting single cell suspensions were obtained as described prior and enriched with the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). This semi-pure cell suspension was stained with fluorescently labelled antibodies via the methods described above and sorted with a 70uM nozzle (RNAseq and Giemsa stainings ...
-
bioRxiv - Cell Biology 2021Quote: ... Magnetic depletion of lineage-positive haematopoietic cells was performed using the EasySep mouse haematopoietic progenitor cell enrichment kit (Stem Cell Technologies), and lineage-depleted stem and progenitor cells were submitted to mass spectrometry analysis ...
-
bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
-
bioRxiv - Immunology 2021Quote: ... primary naïve CD4+ T cells were isolated from the spleen of OT-II mice (B6.Cg-Tg(TcraTcrb)425Cbn/J) using EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) after homogenization with a 70 µm cell strainer and resuspending the cells in PBS supplemented with 2 % FCS and 1 mM EDTA ...
-
bioRxiv - Microbiology 2021Quote: ... 5% CO2 for 15-20 minutes to allow the Matrigel to polymerize and then 550 μl of pre-warmed complete IntestiCult Growth Medium (mouse) (6005; STEMCELL Technologies) containing 500 nM Y-27632 (10005583 ...
-
bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... Isolated cells were grown in a semi-solid methyl cellulose matrix for the Mouse Hematopoietic CFU Assay in Methocult Media 3630 (STEMCELL Technologies), following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: CD8+ T-cells were aseptically isolated from the spleen and lymph nodes of C57BL/6 or FVB (6-8 weeks old) mice using the negative selection Easysep mouse CD8+ T-cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Splenocytes and lung cells were enriched for total B cells using the EasySep Mouse Pan-B Cell Isolation kit (Stemcell Technologies) while whole mln cells were used for downstream processing ...
-
bioRxiv - Cell Biology 2020Quote: ... Adipogenic or osteogenic differentiation of MEFs was induced for 28 days with MesenCult™ Adipogenic Differentiation Kit (Mouse) (STEMCELL Technologies, Canada) and MesenCult™ Osteogenic Stimulatory Kit (Mouse) ...
-
bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens or mesenteric lymph nodes (MLNs) using the EasySep Mouse CD8+ T Cell Isolation Kit (StemCell Technologies). Where indicated ...
-
bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
-
bioRxiv - Immunology 2021Quote: ... CD11b+ monocyte/macrophages were isolated using positive magnetic selection using the EasySep Mouse CD11b Positive Selection Kit II (Stem cell technologies), according to manufacturer’s instructions.