Labshake search
Citations for Stemcell Technologies :
551 - 600 of 605 citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Each well was precoated with 500 ml of Anti-Adherence Rinsing Solution (Stemcell technologies) and spun for 5 minutes at 1500 rcf ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by incubation of the cells with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with a cocktail of lineage specific antibodies (CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and GlyA; Stemcell Technologies) for negative selection of Lin− cells using an EasySep immunomagnetic column (Stemcell Technologies) ...
-
bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...
-
bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
-
bioRxiv - Immunology 2023Quote: ... the spleen of the mouse with the highest antibody titers was dissociated and fused with Sp2/0-Ag14 myeloma cells (ATCC) using the ClonaCell-HY Hybridoma Kit (StemCell Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... that was coated with 2 ml of anti-adherence rinsing solution (Stem cell technologies: 07010) for 5 mins before plating the cells ...
-
bioRxiv - Microbiology 2023Quote: The remaining 180 µL was stained with 20 µL of anti-APC beads (Stemcell technologies, EasySep APC Positive Selection Kit II ...
-
bioRxiv - Immunology 2022Quote: ... was prepared by addition of 0.5ml of Anti-Adherence Rinsing Solution (STEMCELL Technologies, no 07010) and centrifuged at 3,000 x g for three minutes to remove bubbles from the microwells ...
-
bioRxiv - Bioengineering 2024Quote: ... were coated with 500 µl of AggreWell™ Anti-adherence rinsing solution (7010, StemCell Technologies) and rinsed with 2 ml Neurobasal™ medium ...
-
bioRxiv - Neuroscience 2021Quote: ... the sample was sorted using an APC-conjugated antibody to NOTCH1 (eBiosciences #17-5765-80) followed by APC magnetic selection using the EasySep kit (Stem Cell Tech) as per instructions and plated in PneumaCult medium with a SMAD inhibitor ...
-
bioRxiv - Immunology 2023Quote: ... were then seeded overnight on mIL-1β capture antibody coated-coverslips in cRPMI medium supplemented with 10 ng/mL hrM-CSF (Stem Cell Technologies). 30 min before harvesting Mitotracker DeepRed (MTDR ...
-
bioRxiv - Neuroscience 2023Quote: ... plated cells were immediately supplemented with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
-
bioRxiv - Cancer Biology 2023Quote: The purity of cultured CTC subpopulation was validated by gating the sorted cells with conjugated primary antibodies of CD44-FITC (Stem Cell Technologies), CD24-PE ...
-
bioRxiv - Developmental Biology 2021Quote: A 100 μL volume of anti-adherence rinsing solution (StemCell Technologies, Vancouver, Canada, cat. no. 07010) was added to untreated sterile CellStar U-bottom or V-bottom (Figure 1D ...
-
bioRxiv - Neuroscience 2024Quote: ... the microwells were coated with 500 µl of AggreWellTM Anti-adherence rinsing solution (7010, StemCell Technologies) and rinsed with 2 ml NeurobasalTM Plus (A3582901 ...
-
bioRxiv - Developmental Biology 2024Quote: ... and transfer to 10cm plates coated with anti-adherence rinsing solution (Stemcell Technologies, cat. no. 07010). Day 13 ...
-
bioRxiv - Developmental Biology 2024Quote: ... EBs were cultured in 12-well plate coated with anti-adherence rinsing solution (STEM CELL TECHNOLOGIES).
-
bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were then stained for F4/80+ positivity by first using a F4/80-PE conjugated antibody (StemCell Technologies Cat# 60027PE.1) and incubated for 15 min at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... CD31+ EPCs were purified using a FITC-conjugated human CD31 antibody (Miltenyi, cat# 130-117-390, clone AC128) and the EasySep Human FITC Positive Selection Kit II (Stemcell Technologies, cat# 18558). Purified EPCs were seeded into collagen IV (Sigma-Aldrich ...
-
bioRxiv - Genetics 2023Quote: Peripheral blood was collected from Saudi patients into an EDTA-containing blood collection tube and treated with RosetteSepTM Human Progenitor Cell Basic Pre-Enrichment antibody cocktail according to the manufacturer’s instructions (Stem Cell Technologies Catalog#15226). After PBMCs separation and isolation ...
-
bioRxiv - Immunology 2020Quote: ... CAR-transduced T cells were enriched by positive selections using anti-CD19 magnetic beads (Stem Cell Technologies). Cultures were propagated for 14-16 days before applying to assays or get cryo-preserved ...
-
bioRxiv - Bioengineering 2022Quote: ... Each well was rinsed with 1x PBS and an anti-adherence rinsing solution (StemCell Technologies, Aggrewell™) was added into each well ...
-
bioRxiv - Neuroscience 2023Quote: Flow cytometric analysis was performed on healthy control PBMCs after 48h stimulation with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
-
bioRxiv - Immunology 2024Quote: ... cells were stimulated with antibodies against CD3/CD28 (2 µg/mL CD3 and 1 µg/mL CD28 antibodies, ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, or eBioscience) or PMA (20 ng/mL and ionomycin (1 µg/mL ...
-
bioRxiv - Cancer Biology 2021Quote: CD14+ monocytes were selected from frozen human PBMC using anti-CD14 microbeads according to manufacturer’s instructions (Stemcell Technologies). The isolated monocytes were counted and resuspended in growth medium (Stemcell Technologies ...
-
bioRxiv - Developmental Biology 2023Quote: ... Aggrewell™400 was prepared by rinsing the wells with an anti-adherence rinsing solution (Stem cell technologies) as per the manufacturer’s protocol and washed with SL (without LIF ...
-
bioRxiv - Immunology 2024Quote: ... the surface of untreated chips was instead coated with a protein-repellent anti-adherence rinsing solution (STEMCELL Technologies). The solution was directly added to dry and sterile chips ...
-
An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: Samples were depleted of cells of an immune origin with the RosetteSep CD45 depletion kit (depletes with antibodies for CD45, CD66, and Glycophorin A, cat. no. 15122, Stem Cell Technologies, Cambridge, MA USA) according to the protocol with SepMate - 15 tubes (cat ...
-
bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized anti-coagulated blood using density gradient Lymphoprep medium (Stemcell Technologies) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-human CD25-PE-Cy7 (clone MA251) plus FVD-eFluor-780 (eBioscience) and human FcR blocking Reagent (StemCell Technologies). Cells were washed then fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer Set ...
-
bioRxiv - Genetics 2023Quote: ... all plasticware used to handle the cerebral organoids was pretreated with an anti-adhesion solution (Stem Cell Technology #07010). All fixation steps described in this study were performed using 4% paraformaldehyde (PFA ...
-
bioRxiv - Bioengineering 2023Quote: ... wells of the U-bottom 96 well plate were treated with an anti-adherence rinsing solution (STEMCELL Technologies 07010) and centrifuged at 1300g for 5 min ...
-
bioRxiv - Developmental Biology 2022Quote: ... the mold was rinsed once with PBS and then coated with an anti- adherence rinsing solution (StemCell Technologies, # 07010) to increase the non-stick behavior of the PDMS further ...
-
bioRxiv - Cancer Biology 2022Quote: ... Myeloma cells were selected from total bone marrow mononuclear cells using anti-CD138 magnetic bead enrichment (StemCell Technologies, Cat #17887) prior to cryopreservation and use ...
-
bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
-
bioRxiv - Bioengineering 2023Quote: ... and broken down to small fragments by a 200uL pipet tip rinsed with Anti-Adherence Rinsing Solution (07010, STEMCELL Technologies). The tissue fragment suspension was transferred into an Anti-Adherence Rinsing Solution coated 15mL conical tube on ice and allowed to sediment by gravity for at least 5 minutes ...
-
bioRxiv - Immunology 2024Quote: ... irradiated with 7500 cGy) loaded with anti-CD3 mAb (OKT3, UBC AbLab) and cultured in ImmunoCult™ XF (STEMCELL Technologies) supplemented with 100 U/mL penicillin-streptomycin ...
-
bioRxiv - Neuroscience 2024Quote: ... The spheroid-forming wells were prepared prior to cell seeding by adding 0.5 mL of anti-adherence rinsing solution (07010, STEMCELL Technologies) to each well ...
-
bioRxiv - Cancer Biology 2022Quote: ... Neonatal CD34+ HSPCs were obtained from cord blood of healthy donors and enriched using anti-CD34 immunomagnetic microbeads (Miltenyi Biotech and StemCell Technologies). All investigations were performed in accordance to the Declaration of Helsinki and informed consent was obtained according to local laws and regulations ...
-
bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
-
bioRxiv - Molecular Biology 2024Quote: The obtained bone marrow cells were enriched for cells expressing CD11b using anti-CD11b magnetic beads (STEM CELL Technologies, Vancouver, BC), applied according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... non-TC treated 96-well U-bottom plates were passivated with 100 μL anti-adherence rinsing solution (AAR, StemCell Technologies 07010) for 15 min at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... The cells were mechanically detached in VP spheroid medium using a serological pipette before transfer to a 6WP pre-coated with anti-adherence solution (STEMCELL Technologies, 07010). Detached cells from five wells of a 6WP were added divided over six wells of a 6WP ...
-
bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
-
bioRxiv - Developmental Biology 2022Quote: ... EBs were embedded in Matrigel and transferred to 10-cm cell-culture dishes coated with anti-adherence rinsing solution (Stemcell technologies, P2443) in 15ml NI ...
-
bioRxiv - Bioengineering 2024Quote: ... Non-treated petri dishes of 100 mm diameter were coated with 5 mL of anti-adherence rinsing solution (STEMCELL Technologies, Vancouver, CA) for 5 minutes at 37°C to prevent cell adhesion ...