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Citations for Stemcell Technologies :
251 - 300 of 303 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... GBOs cultured for more than one month were dissociated into a single cell with accutase at 37 °C for 30 min and seeded into aggrewell 800 (Stemcell technologies) at a density of 9 × 105 cells/mL with 1 mL of GBO medium supplemented with 10 µM Y27632 ...
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bioRxiv - Genomics 2024Quote: ... in a humidified atmosphere of 5% CO2 at 37°C on low-attachment plates (62) and were dissociated with Accutase (StemCell Technologies) for passaging and plating.
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Cancer Biology 2022Quote: ... glands were first digested overnight (∼16 hours) with gentle agitation at 37 °C in 250 μL Gentle Collagenase (Stemcell Technologies #07919) diluted in 2.25 mL of complete Basal Epicult media formulated according to manufacture instructions (Epicult Basal Medium Stemcell Technologies #05610 ...
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bioRxiv - Immunology 2022Quote: Vials of cryopreserved PBMCs were thawed at 37°C and washed in ClonaCell™-HY Medium A (STEMCELL Technologies, cat. #03801). Epstein-Barr virus (EBV ...
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bioRxiv - Genetics 2022Quote: ... Human ESCs (wild type and ADAR1 KO, gifts from Dr. C. Rice, the Rockefeller University) were maintained in complete mTeSR1 (StemCell Technologies) and the differentiated neural progenitor cells were maintained in STEMdiff Neural Progenitor Medium (StemCell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed with Dulbecco’s Modified Eagle Medium (DMEM/F12) and incubated for 70 min (37°C, 5 % CO2) in STEMdiff Neural Rosette Selection Reagent (StemCell Technologies). Rosettes were lifted by gently dispensing DMEM/F12 onto the colonies ...
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bioRxiv - Physiology 2022Quote: ... Proteases were neutralized with medium containing 10% FBS and resulting suspension of cells were incubated in a flask at 37°C 5% CO2 for 2 hours in PneumaCult-Ex Plus medium (Cat. 05040, Stemcell Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin to remove non-epithelial cells ...
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bioRxiv - Developmental Biology 2023Quote: ... A single-cell suspension was obtained by incubating the dissected embryos at 37°C in a dissociation solution consisting of accutase (Stemcell Technologies) with 3U/mg Papain (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were cultured at 37°C with 5% CO2 and fed every third day by half-media exchange using astrocyte-conditioned BrainPhys media (StemCell Technologies) supplemented with 1% FBS ...
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bioRxiv - Genomics 2024Quote: ... and 100% humidity) in E8 Flex medium (BIONi010-C and BIONi037-A) and mTeSR plus (STEMCELL Technologies, Cat. no 100-0276) (Gibco ...
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bioRxiv - Microbiology 2024Quote: WA09 embryonic stem cells (WiCell, Madison, WI, USA) were expanded and maintained at 37°C/5% CO2 in mTeSR-E8 culture medium (STEMCELL Technologies) on Nunclon cultureware coated with LDEV-free Geltrex (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Undifferentiated iPSCs and iE cells were incubated at 37°C in Gentle Cell Dissociation Reagent (Stem Cell Technologies CAT#100-0485) for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 40 µL of Matrigel containing cells were placed in each well and the plate was incubated at 37 °C for 30 min before adding 500 µL of HepatiCult Organoid Initiation Media (StemCell Technologies). The medium was changed every 2-3 days until the organoid cultures were ready for passaging (∼ 14 days) ...
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bioRxiv - Genetics 2021Quote: ... ∼4 × 105 CS iPSCs containing a c.1569G>A (p.(W523X)) mutation were treated with Accutase (working dilution 1:3 in PBS; StemCell Technologies #07922,). Cells were dissociated to generate a suspension of single cells ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: 1×105 sorted CD34+ HSPCs were seeded in for collagen-based colony-forming assays (MegaCult™-C medium with cytokines, StemCell Technologies), as previously described (Gialesaki et al. ...
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bioRxiv - Cell Biology 2020Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4° C with a mouse monoclonal anti-CD3 antibody (clone UCHT1, StemCell Technologies, #60011) or a mouse monoclonal anti-HA antibody (clone 16B12 ...
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bioRxiv - Cell Biology 2020Quote: ... The hESCs and hiPSCs were maintained under 5% CO2 at 37°C with daily media change and were passaged every 5 to 7 days by ReLeSR (STEMCELL Technologies, 05872) into small clumps and replated onto the precoated culture dishes ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium containing hematopoietic cells and trypsinized cells were collected for FACS analysis and CFU-C assay in Methylcellulose M3434 (Stem Cell Technologies). After 10 days of culture at 37°C in 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 15 min at 37°C and adherent aNSCs/NPCs were lifted from the plate using 1 mL of Accutase (STEMCELL Technologies, 07920) incubated for 5 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Cancer Biology 2023Quote: ... Matrigel was allowed to polymerize at 37 °C for 10-15 minutes before mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies cat#06005), containing Y-27632 (10 μM) ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... on an automated cell-culture platform developed on a Hamilton Microlab STAR Liquid Handling System (Hamilton Company).2,43 Cells were maintained at 37 °C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies 85850, 05876), supplemented with 1% penicillin– streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... DMSO or BI2536 (100 nM, Selleckchem, 72 hours, 37 °C) treated MNs (DIV21) were labelled with NeuroFluor™ NeuO dye (STEMCELL Technologies) with Ex/Em of 468/557 nm that selectively labels neurons in live culture ...
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bioRxiv - Synthetic Biology 2024Quote: WTC11 iPSCs90 were grown with 5% CO2 at 37°C cultured in mTeSR Plus Basal Medium (Stemcell technologies; Cat. No. 100-0276) on Greiner Cellstar plates (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with DMEM (centrifuged at 300 g for 10 mins at 4°C) and further dissociated into single cells using the NeuroCult Chemical Dissociation Kit (STEMCELL Technologies; #05707) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse CAR-19 cells were generated first by isolating T cells from spleens of BALB/c mice followed by CD3 negative selection (Stemcell Technologies #19851A). T cells were activated and transduced with m1928z-mCherry vector as previously described (45).
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bioRxiv - Molecular Biology 2020Quote: ... or β-thalassemia patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies, Vancouver, Canada) as previously described37,38 ...
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bioRxiv - Cancer Biology 2020Quote: GFP positive neural tube regions were dissected after a DispaseI-DMEM/F-12 treatment (Stem cell technologies 1U/ml #07923; 37°C, 30min). Single cell suspensions were obtained by 3 minutes incubation in Trypsin-EDTA 0.05% (Life technologies ...
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bioRxiv - Physiology 2020Quote: ... and maintained at 37 °C with 5% CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver, Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... were grown and maintained at 37°C in hypoxic conditions (3% O2, 5% CO2) in NeuroCult™ NS-A Proliferation medium (STEMCELL Technologies, 05751) supplemented with 1% Heparin Solution (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... enriched lineage negative cells were further purified to obtain an enriched Lin-Sca-1+ c-kit+ (LSK) population using EasySep™ Mouse Sca1 and cKit Positive Selection kits (#18756 & #18757, Stemcell Technologies, CA). For ROS studies ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were prepared by mechanically disaggregating the domes and digesting the Matrigel for 1 hour using dispase at 37°C (Stem Cell Technologies, 7913). Cells were washed in PBS and resuspended in lysis buffer as previously described (Santiago-Gomez et al ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ, Stem Cell Technologies). One well of seeded cells was left untreated as negative control (data not shown) ...
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bioRxiv - Cell Biology 2023Quote: ... PBMCs were cultured at 37°C under 5% CO2 for 5 days in ImmunoCult™-XF T Cell Expansion Medium (STEM CELL Technologies) supplemented with 30 ng/mL human recombinant IL-2 (Peprotech ...
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bioRxiv - Developmental Biology 2024Quote: hiPSCs were thawed in presence of Rock-inhibitor Y-27632 (5µM, Stemgent-Ozyme #04-0012) and cultured under standard conditions at 37°C in mTeSR+ medium (Stem Cell Technologies #100-0276) on Matrigel (Corning ...
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bioRxiv - Developmental Biology 2024Quote: ... The Matrigel droplets were incubated at 37 °C for 30 mins before they were washed into 6-well ultra-low attachment plate (Stem Cell Technologies, 38071) containing STEMdiff kit Expansion media with 1 µM SB431542 and 1 µM CHIR99021 ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Mouse Intestinal Organoids were incubated in 24-well cell culture plates at 37 °C under 5% CO2 in IntestiCult Complete Organoid Medium (#06005, Stem Cell Technologies, Canada) containing CHIR-99021 (3 μM final concentration ...
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bioRxiv - Synthetic Biology 2024Quote: ... HSPCs derived from healthy patients or iPSC-derived HPCs were cultured for 14d at 37°C and 5% CO2 in SFEMII medium (STEMCELL Technologies, Vancouver, Canada) as previously described19,20 ...
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bioRxiv - Bioengineering 2024Quote: ... These cells were maintained in a 5% CO2 incubator at 37°C and were fed every 24 to 48 hours with mTeSR Plus medium (StemCell Technologies, 100-0276). The iPSCs were passaged using the StemPro EZPassage tool (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 103 cells were suspended in 100 µL c-BM media with 1 mL MetholCultTM GF M3434 media (Stemcell Technologies, Vancouver, BC, Canada) and then maintained in culture on a 35-mm plate (Cat# 27150 ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...