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Citations for Stemcell Technologies :
101 - 150 of 303 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... mice were purified with the EasySep Mouse B Cell Isolation Kit (Stemcell Technologies). B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies (Vancouver, Canada). Cells were thawed per manufacturer’s instructions and cultured as previously described.60 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.25 μg/mL amphotericin B) supplemented with 10 μM Y-27632 (STEMCELL Technologies). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Isolation of T and B cells were performed according to the manufacturer’s protocols (Stemcell Technologies).
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bioRxiv - Bioengineering 2021Quote: ... the medium was replaced with RPMI1640 enriched with 5ng/mL PDGF-B (Stem Cell Technologies), 2.5 ng/mL TGF-B (PeproTech) ...
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bioRxiv - Immunology 2022Quote: ... and human B-cell activating factor (BAFF) in 80 mL StemCell medium A (StemCell Technologies) containing 1 μg/mL of cyclosporine A (Millipore-Sigma) ...
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bioRxiv - Genomics 2020Quote: ... B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies; cat ...
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bioRxiv - Immunology 2021Quote: ... and magnetic beads were used for negative selection of bulk B cells (StemCell Technologies, 19054) or naïve B cells (17254) ...
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bioRxiv - Immunology 2024Quote: ... were purified by negative selection using RosetteSep B-cell enrichment Cocktail (StemCell Technologies, Vancouver, Canada) followed by density gradient centrifugation on Lympholite (Cedarlane Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... from day 0 to day 2 and in STEMdiff Medium B (STEMCELL Technologies, Cat# 05310) from day 3 to day 12 to differentiate them into hematopoietic progenitor cells (HPCs) ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated from single cell suspensions by using negative selection kit (Stem Cell Technologies) prior to flow cytometry and cell sorting.
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bioRxiv - Bioengineering 2021Quote: ... The B cells were isolated from fresh blood samples using negative selection kits from Stemcell Technologies (EasySep Direct Human B Cell Isolation Kit) ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Using EasySep mouse B cell enrichment kit and manufacturer’s protocol (StemCell Technologies Inc., Tukwila, WA, USA), B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum ...
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bioRxiv - Cancer Biology 2020Quote: ... Immuno-magnetic T and B cell depletion was performed using the EasySep magnetic system (StemCell Technologies), prior to setting up in vitro cultures ...
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bioRxiv - Developmental Biology 2021Quote: ... CHIR99021 (C, 1 mM; Stem Cell Technologies), (S)-(+)-Dimethindenemaleate (D ...
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bioRxiv - Cell Biology 2024Quote: ... and c-Kit/CD117 antibodies (STEMCELL Technologies) for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... Cells are cultivated in Epicult basal medium with Supplement C (Epicult-C human media kit, Stem Cell Technologies, Vancouver), 5% FCS ...
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bioRxiv - Cell Biology 2020Quote: ... freshly isolated mammary epithelial cells or transduced cells were cultured in complete Epicult-B medium (STEMCELL Technologies) containing 5 % matrigel (Corning) ...
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bioRxiv - Immunology 2020Quote: ... Plasma samples were diluted by half with PBS during RosetteSep human B cell processing (StemCell Technologies #15064), aliquoted ...
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bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
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bioRxiv - Immunology 2024Quote: EasySep Mouse Pan-B cell Isolation and EasySep Release Mouse CD138 Positive Selection kits from STEMCELL Technologies were used to enrich B cells and ASCs from ∼5x107 SPL cells following manufacture guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... Human B cells were then purified by negative selection and magnetic separation (Stem Cell Technologies Cat# 17954). Purified human B cells were pre-activated with anti-human CD40 (clone HB14 ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse B cells were isolated using the EasySEP Mouse CD19 Positive Selection Kit (Stem Cell Technologies #18954). Total cells were collected by centrifugation at 800 g for 5 minutes at 4 °C ...
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bioRxiv - Immunology 2024Quote: B cells subsets were obtained from WT or IL17−/− naïve mice or from mice 14 d after alloantigen immunization (2 × 107 mitomycin C–treated BALB/c splenocytes i.p.) by negative selection (EasySep; StemCell Technologies). Purity of the B cell subset was greater than 98% ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM c- AMP (Stem Cell Technologies Cat # 73886), 20ng/ml BDNF (Stem Cell Technologies Cat # 78005) ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells and CD19+ B cells were negatively isolated using magnetic bead based separation (EasySep, StemCell Technologies). CD14+ monocytes were positively selected (EasySep ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Immunology 2020Quote: ... The mouse B cell isolation kit and mouse naïve CD4+ T cell isolation kit were from STEMCELL technologies. The purified rat anti-mouse α4β7 (DATK32 ...
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bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... minced into small pieces and collected in dissociation media containing Epicult-B Basal medium (Stem Cell Technologies, 05610) supplemented with Epicult B supplement ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mM c-AMP (Stem Cell Technologies Cat # 73886), 20 ng/ml BDNF (Stem Cell Technologies Cat # 78005) ...
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bioRxiv - Genomics 2021Quote: ... MEFs were treated with mitomycin C (StemCell Technologies, #73274) for 2.5-3 hours before they were used as the feeder layer.
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bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were enriched in CD19+ cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) yielding a purity above 95% of CD5+ CD19+ cells ...
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bioRxiv - Cell Biology 2020Quote: ... medium was replaced with STEMdiff™ Hematopoietic Supplement B medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). Cells remained in this medium for 7 additional days ...
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bioRxiv - Cell Biology 2023Quote: FACS purified single cell suspensions (500 cells/per well) were seeded in Human EpiCult-B media (Stem Cell Technologies) supplemented with 5 % FBS on day 0 ...
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bioRxiv - Neuroscience 2024Quote: ... medium was replaced with STEMdiff™ Hematopoietic Supplement B medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). Cells remained in this medium for 7 additional days ...
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bioRxiv - Bioengineering 2024Quote: ... HPCs were diluted to 50,000 cells per well in 100 µL of Media B (STEMdiff Hematopoietic Kit, STEMCELL Technologies) in a 96-well plate ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies, Vancouver, Canada) containing a supplement mix ...
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bioRxiv - Immunology 2022Quote: ... Het and HO mice using EasySep B cell kits by negative selection according to the manufacturer’s instructions (Stemcell Technologies, Canada). B cells were stained with anti CD21-FITC and anti CD23-PE antibodies (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood B cells were isolated via negative selection using RosetteSep immunodensity-based cell separation (Stemcell Technologies, Vancouver, BC, Canada). The purity of CLL/ B cells was analyzed by flow cytometry and revealed that ≥ 90% cells co-expressed CD5/CD19.
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bioRxiv - Microbiology 2022Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the medium was replaced by Stemfit AK02N (A + B) supplemented with CHIR99021 (3 μM, #72052; STEMCELL Technologies, Vancouver, BC, Canada) and ActivinA (10 ng/mL ...
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 (MegaCult®-C Medium without Cytokines, STEMCELL Technologies). Cultures were supplemented with human recombinant 50 ng/ml TPO (Peprotech) ...
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bioRxiv - Microbiology 2020Quote: ... Splenocytes were isolated by shredding the spleen through a mesh followed by washing three times in ClonaCell-HY Medium B (StemCell technologies). Splenocytes were fused to SP2/0 cells using the ClonaCell-HY hybridoma Kit (StemCell cat ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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bioRxiv - Immunology 2022Quote: ... and M2A-GFP KI mice (gift of R. Adelstein, NHLBI/NIH) of either sex using negative selection B cell isolation (StemCell Technologies). Euthanasia was performed in accordance with protocols approved by the National Human Genome Research Institute Animal Use and Care Committee at the National Institutes of Health ...