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Citations for Stemcell Technologies :
151 - 200 of 303 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... ESCs were plated on laminin-coated 6 well plates at a density of 65000 cells per well in N2B27 media (as above but with N2 Supplement-B from StemCell Technologies) supplemented with bFGF (10ng/uL bFGF (R&D) ...
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bioRxiv - Immunology 2020Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymoh nodes using CD19 Microbeads (Miltenyi) or Mouse B cell Isolation Kit (Stemcell Technologies). B cells were stimulated with LPS (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2022Quote: ... Then all cell clumps formed in Stemdiff B were transferred to a 24-well transwell (0.4uM pore, Stem cell technologies, #38024) for further differentiation ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Genetics 2024Quote: Splenic B cells from BACtg and wild type littermate control mice were isolated using the EasySep Mouse B Cell Isolation Kit (StemCell Technologies) and rested for 30 minutes and then either left unstimulated or stimulated with the addition of 10 μg/ml goat anti-IgM F(ab’)2 crosslinking antibody (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... 75 μL of 37°C pre-warmed mTesR1 medium (Stemcell technologies) supplemented 1:10 with CloneR (Stemcell technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... CFU-C assays were performed using Methocult M3434 (Stem Cell Technologies), as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156 ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells using a combined strategy of incorporating primitive hematopoiesis via a WNT switch(77)and sorting and replating CD235a+ cells in Medium B from a commercially available kit (StemCell Technologies #05310). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Neuroscience 2024Quote: ... B cells were then enriched by negative selection using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (17963, Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were washed in PBS and resuspended in PBS containing 2%FBS and 2 mM EDTA before negative selection using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, #19844A) following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: Culture supernatants were prepared by growing 5×107 B cells purified from peripheral blood of either healthy donors or CLL patients by negative selection using RosetteSep™ Human B-cell enrichment Cocktail (#15064; STEMCELL Technologies), in 15 ml RPMI-1640 medium (#8758 ...
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bioRxiv - Immunology 2024Quote: ... 10 x 106 PBMCs were enriched for B cells using CD3 T cell depletion via the EasySep™ Human CD3 Positive Selection Kit II (StemCell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from 2 healthy PBMC donors using the EasySep™ Human B-cell Isolation Kit (Catalog #17954) from StemCell Technologies. Cells were then stained with APC anti-IgD-APC ...
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bioRxiv - Immunology 2024Quote: ... B-cells were then purified from the other harvested PBMCs by negative selection using a Human B-cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Mammary glands were dissected and epithelial cells were isolated via differential centrifugation as previously described.[61] Primary mammary epithelial cells (MECs) were cultured in Epicult-B media with proliferation supplement (Stemcell Technologies, #05610) with 10ng/ml EGF ...
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bioRxiv - Immunology 2024Quote: B cells from tonsils of 5 individuals were enriched using negative selection magnetic beads (EasySep Human B Cell Isolation kit, Stemcell technologies, #17954). They were then pooled to avoid batch effects ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... we performed negative B cell enrichment from 100M PBMCs in each experiment using a commercially available B cell enrichment kit according to manufacturer’s protocol (StemCell Technologies; Vancouver, BC, Canada).
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2022Quote: ... and heat shock (3 hours at 43 □C) in BrainPhys (Stem Cell Technologies) supplemented with 2 mM L-Glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... at 37 °C for 1 min and seeded on AggreWell 800 (#34815; StemCell Technologies) in Essential 8 medium with ROCK inhibitor Y-27632 (10 μM ...
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bioRxiv - Cell Biology 2021Quote: Colony-forming activity (CFU-C) assays were performed using MethoCult M3434 (Stem Cell Technologies). A total of 2×104 BM mononuclear cells (MNCs ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated overnight at 4° C with anti-CD3 (Clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C and passaged every 5-6 days using ReLeSR (Stem Cell Technologies).
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bioRxiv - Neuroscience 2023Quote: ... 200 μL of pre-warmed (37°C) Intesticult Media (Stem Cell Technologies, Cat#06005) containing Primocin ...
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bioRxiv - Cell Biology 2023Quote: ... coated plates and dissociated into single cells using 37°C Accutase (Stem Cell Technologies) for ∼5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then triturated in prewarmed (37 °C) trypsin-EDTA (0.25%; STEMCELL Technologies, 07901) with a 1 mL pipette for 2 min ...
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bioRxiv - Developmental Biology 2022Quote: ... dissociated into single cells (accutase, Stemcell Technologies, 07920, 30-60 min, 37 °C, 5% CO2) and fixed (Foxp3/Transcription Factor Staining Buffer Set ...
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bioRxiv - Genomics 2022Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37°C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours ...
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bioRxiv - Genomics 2021Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37°C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours.
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bioRxiv - Cancer Biology 2023Quote: ... H1 cells were cultured in Matrigel (Corning)-coated plates at 37 °C and 5% CO2 using mTeSR-1 Basal Medium (STEMCELL Technologies) exchanged every 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured at 37°C with 5% CO2 in StemSpan (Stem Cell Technology #09650) supplemented with TPO (0,125ng/mL) ...
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bioRxiv - Immunology 2024Quote: ... The tissue segments were incubated at 37°C with Gentle Cell Dissociation Reagent (Stemcell Technologies) for 5 minutes with shaking at 100 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... coated culture dishes for 1h at 37°C with 5% CO2 using mTeSR1 medium (STEMCELL technologies). Cultures were routinely tested for mycoplasma contamination using MycoAlert Kit (Lonza) ...
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bioRxiv - Developmental Biology 2021Quote: ... coated dishes at 37°C under hypoxia conditions (10% CO2/5%O2) in mTeSR1 (StemCell Technologies). These were passaged every 4-6 days with Accutase (#A6964 ...
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bioRxiv - Cancer Biology 2020Quote: Primary FL cell-suspensions were defrosted at 37°C and layered onto 3ml of lymphoprep (STEMcell Technologies). Lymphocytes were isolated by centrifugation at 1150g for 12 minutes ...