Labshake search
Citations for Stemcell Technologies :
1351 - 1400 of 1403 citations for Cow UMP CMP kinase CMPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
-
bioRxiv - Immunology 2020Quote: ... Each antibody clone and lot was titrated to optimal staining concentrations using primary murine samples with all appropriate positive and negative controls: polyclonal murine CD8 T cells purified by positive selection kit (Stem Cell Technologies, Vancouver, Canada) stimulated with PMA/Ionomycin via eBioscience Cell Stimulation Cocktail (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... CD45 positive cells were depleted from tumor cell digests using the EasySep ™ Mouse streptavidin Rapidspheres™ Isolation Kit (Stem Cell Technologies, Catalog #19860) and a biotin conjugated anti Mouse CD45 antibody (Stem Cell Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... We confirmed our iPSC cell banks have normal copy number at various mutation hotspots using the hPSC Genetic Analysis Kit (Cat # 07550, STEMCELL Technologies, Vancouver, BC, CAN).
-
bioRxiv - Cell Biology 2020Quote: Depletion of lineage-committed cells from single-cell suspensions of mouse bone marrow was performed using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, BC, Canada).
-
bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
-
bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
-
bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
-
bioRxiv - Immunology 2021Quote: ... CD34+ peripheral stem cells were isolated from peripheral blood and expanded using the Complete Kit for Human Whole Blood CD34+ Cells (Stem Cell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2022Quote: ... the cells were dissociated and plated at a density of 3×104 cells/cm in STEMdiff™ Neuron Maturation Kit (Stem Cell Technologies, #08510) medium on a double layer of poly-L-ornithine (Sigma-Aldrich #P3655 ...
-
bioRxiv - Bioengineering 2022Quote: ... CD4+ T cells were isolated from the spleen of C57/BL6 mice using the CD4 T cell isolation kit from Stemcell Technologies (# 19852; Cologne, Germany) and subsequently naïve T helper cells (CD3+ ...
-
bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
-
bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
-
bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
-
bioRxiv - Immunology 2023Quote: ... Purification of CD8+ T cells for sorting or cell culture was performed using a mouse CD8+ T cell isolation kit (Stem Cell Technologies, Cat# 19853), following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: An Easy Step Human CD8+ T Cell Isolation Kit was used to isolate CD8+ T cells from a donor PBMC sample via negative selection (Cat #, 17913, Stem Cell Technologies, Vancouver, Canada).
-
bioRxiv - Immunology 2023Quote: ... Intermediate layer containing IELs was collected and washed with 1X PBS followed by either flowcytometry analysis or used for CD8α IELs purification using EasySep™ mouse CD8α positive selection kit-II (StemCell Technologies; Cat# 18953). Sort purified CD8α IELs (50,000/mouse ...
-
bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
-
bioRxiv - Neuroscience 2023Quote: Neural progenitor cells (NPCs) were created from the hESCs described above using the STEMdiff SMADi Neural Induction kit from STEMCELL Technologies (Cat No. 08581) according to their guidelines for Matrigel-coated 6-well plates [31] ...
-
bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... The cells were differentiated and matured using the StemCell Midbrain Neuron differentiation and maturation kit (#100-0038 and #100-0041; StemCell Technologies, New York, NY). Briefly ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell pellet was resuspended in 500 µL HBBS with 0.5% fatty acid-free BSA and lymphocytes were isolated by using the EasySep Mouse Streptavidin RapidSpheres isolation kit (STEMCELL Technologies, Vancouver, British Columbia, Canada) with the protocol provided ...
-
bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were isolated from healthy donor-derived PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. # 17953). CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: Primary human CD4 and CD8 T cells were isolated from PBMCs by negative selection using STEMCELL EasySep isolation kits (Stemcell Technologies, 17952 and 17953), and mixed 1:1 ...
-
bioRxiv - Immunology 2024Quote: ... Dendritic cells (DCs) were isolated from spleens of wild type mice using EasySep™ Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies, 19863). Co-cultures were set up in 4 replicates with BMDM primed as above ...
-
bioRxiv - Immunology 2024Quote: ... OT-I T cells (CD8+) were isolated from spleens of OT I mice using EasySep™ Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies, 19853A). Dendritic cells (DCs ...
-
bioRxiv - Biochemistry 2024Quote: ... PBMCs were isolated from the blood of healthy volunteer and enriched for CD3+ Tcells using EasySep™ human T-cell isolation kit (Stemcell Technologies, Vancouver,Canada) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... CD4+CD25+ T cells were isolated from splenocytes of DR3 and DR3.IL17A-/- transgenic mice using EasySep™ Mouse CD4+CD25+ regulatory T cell isolation kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
-
bioRxiv - Immunology 2024Quote: CD4+CD25+ T cells isolated from the spleens of DR3 and DR3.IL17A-/- transgenic mice using the EasySep™ Mouse CD4+CD25+ regulatory T Cell Isolation Kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
-
Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... The pluripotency of C43-1260 was tested by in vitro differentiation to the three germ layers using the STEMdiff™ Trilineage Differentiation Kit (STEMCELL Tech, Catalog # 05230). All iPSCs were cultured and expanded in mTeSR™ Plus medium (STEMCELL Tech ...
-
bioRxiv - Genomics 2019Quote: CD4+ T cells were enriched from PBMCs by negative MACS selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies SARL, Grenoble, France), according to manufacturer’s instructions.
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
-
bioRxiv - Microbiology 2020Quote: ... Primary human B cells were isolated by negative selection using RosetteSep Human B Cell Enrichment and EasySep Human B cell enrichment kits (Stem Cell Technologies, #15064 and #19054), according to the manufacturers’ protocols ...
-
bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
-
bioRxiv - Immunology 2022Quote: ... successfully transduced cells were enriched based on CD19t expression on day 11-15 using EasySep™ Human CD19 Positive Selection Kit II (Stemcell Technologies, Inc., Cat#17854), and the resulting CAR+ T cells were cryopreserved at day 15 or 17 ...
-
bioRxiv - Neuroscience 2019Quote: ... splenocytes were further separated by negative selection using the EasySep T cell isolation kit designed for CD3+ T cells (Stem Cell Technologies, Vancouver, BC, Canada) following the manufacturer’s guidelines for manual separation ...
-
bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
-
bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
-
bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: Transduced lineage negative (Lin-) or Lin-Sca-1+Kit+ (LSK) cells were plated into 1mL of methylcellulose-based medium with cytokines (Stem Cell Technology, MethoCult GF M3434) into 35 x 10 mm non-adherent Bio-One Petri Dish ...
-
bioRxiv - Neuroscience 2023Quote: Astrocyte precursor cells were created from the neural progenitor cells described above using the STEMdiff Astrocyte Differentiation media kit from STEMCELL Technologies (Cat No. 100-0013) following their protocol for Matrigel-coated 6-well plates [30] ...
-
An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: Samples were depleted of cells of an immune origin with the RosetteSep CD45 depletion kit (depletes with antibodies for CD45, CD66, and Glycophorin A, cat. no. 15122, Stem Cell Technologies, Cambridge, MA USA) according to the protocol with SepMate - 15 tubes (cat ...
-
bioRxiv - Cancer Biology 2023Quote: The first step consists in a CTC enrichment from whole blood sample by immunomagnetic negative selection using the EasySep™ Direct Human CTC Enrichment Kit (StemCell Technologies®, Vancouver, Canada) according to manufacturer instruction ...
-
bioRxiv - Bioengineering 2024Quote: ... PBMC samples were thawed on the day of activation and T cells were purified via the EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies Inc, cat#19053). Isolated T cells were seeded in the CD3/CD28 precoated well plates at a concentration of 500,000 cells/mL with 1 mL/well in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies Inc ...
-
bioRxiv - Immunology 2024Quote: T cells were extracted from OT-II mouse spleens and magnetically sorted using a T-cell extraction kit from Stem Cell Technologies (catalog number 19851). Cells were incubated in culture media (RPMI+ 10% HIFBS + Pen/Strep + 1 mM HEPES + 50 μM beta mercaptoethanol ...
-
bioRxiv - Cell Biology 2024Quote: Primary human T cells were isolated from the peripheral blood of a healthy donor using a negative selection kit (EasySep, STEMCELL Technologies Canada Inc., Vancouver, BC) and used for testing the sensitivity of the lifetime flow system to T cell activation ...
-
bioRxiv - Immunology 2024Quote: ILCs from Alt and Alt plus BPC treated mice were enriched from two single-cell suspended lungs by Mouse Pan-ILC Enrichment Kit (STEMCELL Technologies, Vancouver, British Columbia, Canada). ILC enriched samples were preserved in PIPseqTM T100 3L Single Cell Capture and Lysis Kit and processed by sequencing service (Fluent BioSciences ...