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Citations for Stemcell Technologies :
951 - 1000 of 1403 citations for Cow UMP CMP kinase CMPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
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bioRxiv - Immunology 2023Quote: ... France) were processed for the isolation of CD34+ precursor cells using the EasySep Human CD34 Positive Selection Kit (Stemcell Technologies). Isolated cells were cultured for 1-2 weeks in StemSpan Medium (Stemcell Technologies ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cell culture was done using a commercially available media kit (mTeSR Plus; Cat No. 100-0276, STEM CELL Technologies) following the manufacturer’s published protocol [32] ...
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bioRxiv - Cell Biology 2023Quote: ... The timely collection of the CMs was done using STEMdiff reagent from the CM dissociation kit (STEMCELL Technologies, Vancouver, BC). STEMdiff was added to each well of hiPSC-CMs ...
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bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
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bioRxiv - Bioengineering 2023Quote: Organoids were dissociated into a single-celled suspension using a modified protocol using the STEMdiff Cardiomyocyte Dissociation Kit (STEMCELL Technologies). Upon being transferred to a microcentrifuge tube ...
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bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD8+ Tn or Teff cells isolated from LmOVA-infected mice by Thy1.1 positive selection kit (StemCell Technologies, Vancouver, BC, Canada) and were cultured immediately ex vivo in Seahorse XF medium (non-buffered DMEM 1640 containing 25mM glucose ...
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bioRxiv - Immunology 2023Quote: ... and cells were counted using a hemocytometer and dead cells were removed with a magnetic bead based dead cell removal kit according to the manufacturer’s instructions (EasySep STEMCELL technologies). Following lung digestion 1/4 of the cell suspension was sorted for CD45+ CD3- CD19- Ly6G- FceRIa- CD5- NK1.1- CD127+ CD90+ ILCs and spiked back into the whole lung single cell solution ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from PBMC by depletion of non-NK cells using an NK cell isolation kit (STEMCELL Technologies). The manufacturer’s protocol was modified as follows ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were purified by negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes ...
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bioRxiv - Immunology 2023Quote: Monocytes were isolated by depletion of non-monocytic cells using a human monocyte enrichment kit without CD16 depletion (STEMCELL Technologies). PBMC were resuspended at 5ξ107 cells/mL and 50 μL/mL of the non-monocyte cocktail was added ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
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bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
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bioRxiv - Developmental Biology 2023Quote: ... Clones with the insertions were then tested for common chromosomal abnormalities using the hPSC Genetic Analysis Kit (STEMCELL Technologies Inc.).
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
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bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and used directly for total CD4+ T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Developmental Biology 2023Quote: ... the H1 ES cell line was differentiated to PP cells using the STEMdiff™ Pancreatic Progenitor Kit (STEMCELL Technologies, 05120) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... NK cells were purified by negative selection by using the EasySep™ Human NK cell Enrichment Kit (Stem Cell Technologies). 5×104 NK were co-cultured with NK sensitive cell line ...
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bioRxiv - Microbiology 2023Quote: Dorsal forebrain regionalized neural organoids (RNOs) were generated using the STEMDiff™ Dorsal Forebrain Organoid Differentiation Kit (STEMCELL Technologies™) and STEMDiff™ Neural Organoid Maintenance Kit (STEMCELL Technologies™ ...
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bioRxiv - Immunology 2023Quote: ... T cells are isolated using an EasySep™ Human T Cell Enrichment Kit as described by the manufacturer (Stemcell Technologies). For T cell activation ...
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bioRxiv - Immunology 2024Quote: ... The samples were enriched for CD4 T cells using the EasySep™ mouse CD4+ T Cell isolation kit (Stemcell technologies) and the dead cells were removed using EasySep™ Dead Cell Removal (Annexin V ...
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bioRxiv - Cell Biology 2024Quote: F814 and WT bone marrow cells were cultured for 10 days in MesencultTM Expansion Kit for mouse (Stemcell tech, 05513) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and P14 cells were isolated on 7 dpi using the EasySep Mouse APC Positive Selection Kit II (#17667, STEMcell technologies) with a CD45.2 APC antibody (#17-0454-82 ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were enriched from total splenocytes using the EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) (routinely >90% purity).
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bioRxiv - Immunology 2024Quote: ... Neutrophils from an unidentified donor were obtained from whole blood using EasySep™ Direct Human Neutrophil Isolation Kit (StemCell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Single cell suspensions of BMMNCs were first lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). Lineage depleted cells were then stained with ...
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bioRxiv - Microbiology 2024Quote: ... Monocytes were isolated from PBMCs using EasySep™ Human CD14 Positive Selection Kit according to the manufacturer’s instructions (Stemcell Technologies).
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bioRxiv - Immunology 2024Quote: Naïve CD4+ or CD8+ T cells were isolated from mouse spleen and lymph nodes by using a mouse naïve CD4+ or CD8+ T cell isolation kit (STEMCELL Technologies). Purified naïve CD4+ or CD8+ T cells were labeled with CellTrace Violet (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells were purified from mouse spleen and lymph nodes by using a mouse naïve CD4+ T cell isolation kit (STEMCELL Technologies) and polarized following the manufacturer’s protocol for Immunocult Mouse Th1 or Th2 differentiation supplements (STEMCELL Technologies) ...
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bioRxiv - Neuroscience 2024Quote: hiPSCs were differentiated into iMGLs as described previously with minor adaptations.52,73 hiPSCs were differentiated into hematopoietic precursors cells (HPCs) using the STEMdiff Hematopoietic kit (STEMCELL Technologies) largely as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... where randomly grouped and 2 x 106 naïve Ly5.2+CD8+CD44loCD62Lhi OT-I T cells [purified by FACS or using the EasySep™ murine naïve CD8+ T cell isolation kit (Stemcell Technologies)] were injected intravenously ...
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bioRxiv - Cell Biology 2019Quote: ... at a density of 9000 cells per well in 100 μl of Embryoid Body Formation Medium (STEMdiff Cerebral organoid Kit, STEMCELL Technologies) with either addition of 10 μM Y-27632 (Tocris ...
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bioRxiv - Cell Biology 2020Quote: ... The buffy coat was first separated by Ficoll-Paque Plus solution and the NK cells were then purified from the Peripheral Blood Mononuclear Cells (PBMCs) by negative selection using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from the spleens of anemic mice using the EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... peripheral blood monocytes (PBMCs) were isolated from the whole blood of healthy donors using an EasySep Direct Human PBMC Isolation Kit (StemCell Technologies) through negative selection ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... cardioids were maintained in CDM with insulin.21,31 Samples were collected 7 days post induction and dissociated using the STEMdiff cardiomyocyte dissociation kit (#05025, Stem Cell Technologies) and subsequently washed 3x with PBS.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated from buffy coats from healthy donors using the RosetteSep NK cell enrichment kit (STEMCELL Technologies, MA, USA) and stimulated with rhIL-15 (1ng/ml ...
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bioRxiv - Immunology 2021Quote: For sorting single cell suspensions were obtained as described prior and enriched with the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). This semi-pure cell suspension was stained with fluorescently labelled antibodies via the methods described above and sorted with a 70uM nozzle (RNAseq and Giemsa stainings ...
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bioRxiv - Immunology 2019Quote: OT1 and OTII T cells were isolated from LN of TCR transgenic mice using either a CD8 or CD4 StemSep enrichment kit (STEMCELL Technologies), respectively ...