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Citations for Stemcell Technologies :
1051 - 1100 of 1403 citations for Cow UMP CMP kinase CMPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... were isolated from perioperative human patients’ brain tissues (Nervous System Tumor Bank) using EasySep Human CD8 T Cells Isolation Kit (STEMCELL Technologies) following the tissue digestion as previously described [68 ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction remaining after the positive selection of CD14+ cells was used for pan-DC enrichment employing the EasySep human pan-DC enrichment kit (StemCell Technologies). Total pan-DC were resuspended with 20,000 CD14+ cells and sent for sequencing ...
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bioRxiv - Immunology 2021Quote: ... 100μL of Isolation Cocktail and 100μL of Rapid Spheres (Easy Sep™ Direct Human Total Lymphocytes Isolation Kit: 19655, StemCell Technologies) were added to the remaining whole blood ...
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bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
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bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: Monocytes were enriched from the PBMCs of healthy individuals by using the EasySep CD14 positive selection kit according to the manufacturer’s instructions (STEMCELL Technologies, Canada). Monocytes were cultured in the DC medium for 6 days ...
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bioRxiv - Immunology 2020Quote: ... Total CD3+ T cells were isolated from unfractionated PBMCs using the EasySep™ Human T Cell Isolation Kit (Stem Cell Technologies) with a DynaMag™-2 magnet (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Preliminary experiments were also performed after isolation of monocytes using the EasySep human monocyte enrichment kit with CD16 depletion (StemCell Technologies) and the EasySep human monocyte isolation kit (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... infection efficiency was determined by GFP expression and infected cells were isolated using RoboSep mouse CD8a positive selection kit II (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... In vitro experiments were performed using human plasmacytoid dendritic cells (pDCs) purified from PBMCs by negative selection with the EasySep Human Plasmacytoid DC enrichment kit (STEMCELL Technologies). pDCs were cultured in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Memory human CD8+ T cells were purified from healthy donor blood samples using the EasySep Human Memory CD8+ T cell enrichment kit (StemCell Technologies), cells were routinely ∼90% CD8+RO+RA-.
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bioRxiv - Immunology 2020Quote: Total human CD8+ T cells were purified from healthy donor blood samples using the EasySep Negative human CD8+ T cell isolation kit (StemCell Technologies). CD8+ T cells were routinely ∼90% pure ...
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bioRxiv - Immunology 2020Quote: ... Total mDC and pDC were negatively enriched from PBMCs using the EasySep™ Human Pan-DC Pre-Enrichment Kit (StemCell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Total T-cells were purified from the same donor’s PBMC using the EasySep™ Human T cell Isolation kit (StemCell Technologies). Purity of total CD4+ and CD8+ T-cells was ∼94% (Supplementary Fig 2) ...
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bioRxiv - Cell Biology 2020Quote: Peripheral blood was collected from a healthy volunteer into a BD Vacutainer tube and mononuclear cells were isolated using the SepMate kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Human ES cells were differentiated using the STEMdiff Definitive Endoderm Kit (TeSR-E8 Optimized) from STEMCELL Technologies (Ramme et al. 2019). Rat ES cells were differentiated following an optimized mouse protocols (Kim et al ...
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bioRxiv - Immunology 2021Quote: ... CD90.2+ cells were pre-sorted from Rag2−/− lung and bone marrow single cell suspensions using an EasySep™ Mouse CD90.2 Positive Selection Kit II (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... WT or Rnf5−/– Lin−Sca1+c-Kit+ cells transformed with GFP-MLL-AF9 were resuspended in methylcellulose M3234 (Stem Cell Technologies) supplemented with 6 ng/mL IL-3 ...
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bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
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bioRxiv - Immunology 2022Quote: ... NK cells were either isolated from cryopreserved PBMCs by either negative selection using the EasySep NK cell enrichment kit (StemCell technologies) or by depletion of T cells with human CD3 MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells from spleens were negatively enriched using an EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853) according to the manufacturer’s instructions (STEMCELL Technologies). Enriched CD8+ T cells (92% - 96% purity ...
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow plasma cells (BMPC) were enriched from bone marrow mononuclear cells using the EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% DMSO in FBS.
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bioRxiv - Molecular Biology 2022Quote: ... Neutrophils were isolated from 20 ml of blood with EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, British Columbia, Canada, 19666) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... apoptotic and dead cells were removed using immunomagnetic cell separation with the Annexin Dead Cell Removal Kit (StemCell Technologies, Cat# 17899) and EasySepTM Magnet (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated from spleens and lymph nodes of P14 Tcf7GFP or OTI Tcf7GFP mice using the EasySepTM Mouse CD8+ T cell Isolation Kit (Stemcell Technologies), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Splenic naïve CD4+ T cells were negatively selected using an EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naïve T cells were resuspended in supplemented RPMI 1640 medium with penicillin (100 U/ml) ...
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Immunology 2022Quote: ... or GZB-cre Zbtb20-fl/fl CD45.1 OT-I mice (KO) and naive CD8 T cells purified using EasySep Mouse Naive CD8 T Cell Isolation Kits (catalog no. 19858A; STEMCELL Technologies). Fifty thousand naive OT-I cells were retro-orbitally injected into congenic B6 recipient mice ...
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bioRxiv - Microbiology 2022Quote: ... Loosely adherent immature BMDCs were collected and then CD11c+ DCs were enriched using CD11c positive selection kit (Stemcell Technologies, Cambridge, MA). Twenty-four hours after culture of purified subsets ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... primary CD4+ T-cells were negatively enriched using EasySep Human CD4+ T-cell isolation kit (Catalog no. 17952; Stem Cell Technologies) and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2 ...
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bioRxiv - Molecular Biology 2022Quote: H9 human ES cell lines obtained from the HMS Cell Biology Initiative for Genome Editing and Neurodegeneration were cultured using the mTeSR1TM1 kit (STEMCELL technology) and 1% penicillin-streptomycin (Sigma ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from spleens and lymph nodes using the EasySep mouse CD4+ T cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were negatively selected from single cell suspensions using the EasySep™ CD4+ T cell isolation kit (Stemcell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Spleens were processed into single cell suspensions and CD4+ T cells were isolated using the EasySep CD4+ T cell isolation kit (StemCell Technologies) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Human naïve T cells were isolated from PBMCs using the EasySep™ Human naïve CD4+ T Cell Isolation Kit Human (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... we took advantage of the STEMdiff™ trilineage differentiation Kit and followed the manufacturer’s instructions with minor modifications (Cat Nº 05230, Stemcell technologies). hiPSCs were cultured onto Matrigel-coated glass coverslips and on day 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were obtained at >95% purity from pooled collections using a two-step Rosette-Sep/EasySep human CD34-positive selection kit (STEMCELL Technologies) according to the manufacturer’s protocols and/or FACS sorting ...
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2024Quote: The CD4+ T-cell subpopulation was isolated from the CD14- fraction of PBMCs using the Easysep human CD4+ T-cell isolation kit (Stemcell Technology) as per the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada). Second- generation 4-1BB- costimulated TSHR CAR constructs were synthesized de novo (IDT ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM EDTA buffer and monocytes were then isolated using the magnetic EasySep Human CD14+ Selection kit (STEMCELL Technologies, Vancouver, BC, Canada) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, France) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, Canada). Culture supernatants were screened using ELISA (see below ...
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bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
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bioRxiv - Neuroscience 2024Quote: ... 70,000-80,000 EPSCs were used for the development of individual organoids using the STEMdiff™ Cerebral Organoid Kit (Stemcell technologies, #08570) and the STEMdiff™ Cerebral Organoid Maturation Kit (Stemcell technologies ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from splenocytes by the EasySep Mouse CD4+ T-cell Isolation Kit (#19852, STEMCELL Technologies, Vancouver, Canada) were used to evaluate the capability to differentiate into different T cell subsets as previously described [18,19,20] ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from spleens of P14 donor mice by negative selection using the EasySepTM Mouse CD8+ T-cell isolation kit (STEMCELL Technologies). P14 cells were activated in vitro for 24-28 hours with 100U/mL recombinant IL-2 ...