Labshake search
Citations for Stemcell Technologies :
1051 - 1100 of 1509 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Monocytes were isolated by negative selection using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (StemCell Technologies, Cambridge, MA) according to factory instructions ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were enriched from total splenocytes using the EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies) (routinely >90% purity).
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bioRxiv - Immunology 2024Quote: ... Neutrophils from an unidentified donor were obtained from whole blood using EasySep™ Direct Human Neutrophil Isolation Kit (StemCell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Single cell suspensions of BMMNCs were first lineage depleted using EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies). Lineage depleted cells were then stained with ...
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bioRxiv - Microbiology 2024Quote: ... Monocytes were isolated from PBMCs using EasySep™ Human CD14 Positive Selection Kit according to the manufacturer’s instructions (Stemcell Technologies).
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bioRxiv - Immunology 2024Quote: Naïve CD4+ or CD8+ T cells were isolated from mouse spleen and lymph nodes by using a mouse naïve CD4+ or CD8+ T cell isolation kit (STEMCELL Technologies). Purified naïve CD4+ or CD8+ T cells were labeled with CellTrace Violet (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells were purified from mouse spleen and lymph nodes by using a mouse naïve CD4+ T cell isolation kit (STEMCELL Technologies) and polarized following the manufacturer’s protocol for Immunocult Mouse Th1 or Th2 differentiation supplements (STEMCELL Technologies) ...
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bioRxiv - Neuroscience 2024Quote: hiPSCs were differentiated into iMGLs as described previously with minor adaptations.52,73 hiPSCs were differentiated into hematopoietic precursors cells (HPCs) using the STEMdiff Hematopoietic kit (STEMCELL Technologies) largely as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Cells were differentiated to NPCs using STEMdiff™ SMADi Neural Induction Kit following the monolayer culture protocol (STEMCELL Technologies 08582). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Total CD4 T cells were isolated from PBMC using EasySep™ Human CD4+ T Cell Isolation Kit (#17952, StemCell Technologies) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and total CD4+ T-cells were obtained via negative immunomagnetic selection (EasySep Human CD4+ T-cell Enrichment Kit, Stemcell Technologies), with cell count ...
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bioRxiv - Bioengineering 2024Quote: ... Bavarian Red Cross) obtained from healthy and anonymous donors using EasySep T Cell Isolation Kit (cat. # 17951, Stem Cell Technologies). For CAR-T cell generation ...
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bioRxiv - Cell Biology 2024Quote: ... and dissociated into single cells using the NeuroCult Enzymatic Dissociation Kit for Adult CNS Tissue (Stem Cell Technologies cat. 05715) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: T cells were isolated from PBMCs of healthy donors using the EasySep™ Human T Cell Isolation Kit (STEMCELL Technologies). They were activated for 24 hours at 37°C and 5% CO2 in Hepes-buffered RPMI medium containing 20 IU/mL recombinant human IL-2 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were differentiated into CD-43 expressing hematopoietic progenitor cells (HPCs) using the STEMdiffTM Hematopoietic Kit (cat. 05310, STEMCELL Technologies) for 12 days ...
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bioRxiv - Cancer Biology 2024Quote: ... samples underwent selection for CD34-positivity using a CD34 MicroBead Kit (Miltenyi Biotech) and cultured in StemSpan SFEM (STEMCELL Technologies) supplemented with 100ng/ml hTPO ...
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bioRxiv - Genetics 2024Quote: ... were thawed and T cells were isolated using EasySep Buffer and the EasySep Human T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Placenta cell suspensions were suspended in Iscove’s MDM with 2% fetal bovine serum and cultured using a MethoCult GF M3434 Optimum kit (STEMCELL Technologies). 10,000 cells from each sample were plated in 35-mm culture dishes (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... where randomly grouped and 2 x 106 naïve Ly5.2+CD8+CD44loCD62Lhi OT-I T cells [purified by FACS or using the EasySep™ murine naïve CD8+ T cell isolation kit (Stemcell Technologies)] were injected intravenously ...
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bioRxiv - Cell Biology 2020Quote: ... The buffy coat was first separated by Ficoll-Paque Plus solution and the NK cells were then purified from the Peripheral Blood Mononuclear Cells (PBMCs) by negative selection using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from the spleens of anemic mice using the EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... peripheral blood monocytes (PBMCs) were isolated from the whole blood of healthy donors using an EasySep Direct Human PBMC Isolation Kit (StemCell Technologies) through negative selection ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... cardioids were maintained in CDM with insulin.21,31 Samples were collected 7 days post induction and dissociated using the STEMdiff cardiomyocyte dissociation kit (#05025, Stem Cell Technologies) and subsequently washed 3x with PBS.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated from buffy coats from healthy donors using the RosetteSep NK cell enrichment kit (STEMCELL Technologies, MA, USA) and stimulated with rhIL-15 (1ng/ml ...
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bioRxiv - Immunology 2021Quote: For sorting single cell suspensions were obtained as described prior and enriched with the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). This semi-pure cell suspension was stained with fluorescently labelled antibodies via the methods described above and sorted with a 70uM nozzle (RNAseq and Giemsa stainings ...
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bioRxiv - Genomics 2020Quote: ... Lymph nodes were harvested from a 6-week-old GFP OT-1 mouse and CD8 T cells were isolated using an EasySep mouse CD8 T cell negative selection kit (StemCell Technologies). 14 days post tumor injection ...
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bioRxiv - Immunology 2021Quote: ... Primary CD4+ or CD8+ T cells were isolated using EasySep Human CD4+ or CD8+ T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI 1640 (ATCC ...
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bioRxiv - Cell Biology 2021Quote: ... Magnetic depletion of lineage-positive haematopoietic cells was performed using the EasySep mouse haematopoietic progenitor cell enrichment kit (Stem Cell Technologies), and lineage-depleted stem and progenitor cells were submitted to mass spectrometry analysis ...
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bioRxiv - Immunology 2021Quote: ... primary naïve CD4+ T cells were isolated from the spleen of OT-II mice (B6.Cg-Tg(TcraTcrb)425Cbn/J) using EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) after homogenization with a 70 µm cell strainer and resuspending the cells in PBS supplemented with 2 % FCS and 1 mM EDTA ...
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bioRxiv - Bioengineering 2021Quote: ... CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies). Isolated T cells were then plated in ImmunoCult-XF T Cell Expansion Medium (StemCell Technologies ...
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bioRxiv - Bioengineering 2020Quote: HSPC (CD34+) were isolated from cord blood using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) according to the manufacture’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... Human pluripotent stem cell (hPSC)-derived cerebral organoids were grown at 37°C with 5% CO2 in STEMdiff Cerebral Organoid Kit cell culture media from STEMCELL Technologies Inc ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from healthy adult PBMC using a RosetteSep NK Cell Enrichment Kit (Stem Cell Technologies, Vancouver, BC, Canada), according to manufacturer’s instructions followed by density gradient centrifugation in Histopaque ...
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bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... The CD4+ T cells were purified from 50 × 106 PBMCs using EasySep™Human CD4+ T cell isolation kit (STEMCELL Technologies). Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v ...
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bioRxiv - Immunology 2021Quote: Naïve IgM+IgD+B cells were purified by negative selection using the EasySepTM human naive B cell enrichment kit (19254; StemCell Technologies) from healthy subject PBMCs ...
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bioRxiv - Molecular Biology 2020Quote: ... and LN cells were enriched for CD4+ T cells using the EasySep NHP CD4+ T cell isolation kit from STEMCELL Technologies Canada Inc (Cat# ...
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bioRxiv - Microbiology 2021Quote: ... buffy coats (from anonymous healthy blood donors from Mississippi Blood Center) were separated by Ficoll and CD4+ T-cells purified using RosetteSep Human CD4+ T-cell enrichment kit (STEMCELL Technologies). CD4+ T-cells cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI ...
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bioRxiv - Microbiology 2020Quote: A lentiviral vector encoding anti-HIV-1 CAR was transduced and expressed in the enriched total human T cells obtained from fresh peripheral blood mononuclear cells by negative selection (EasySep Human T cell isolation kit, Stemcell Technologies). CAR-T cells were maintained in IMDM supplemented with 20% FBS ...
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bioRxiv - Microbiology 2020Quote: ... DCs and naïve CD4+ T cells were harvested from SPLNs of mice at 7 weeks of age using a CD11c positive selection kit II (STEMCELL Technologies) and a mouse naïve CD4+ T cell Isolation kit (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Lin−Sca-1+c-Kit+CD150+CD48− (HSC) or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) populations were cultured in Myelocult M5300 (STEMCELL Technologies) with 100 µg/ml Primocin (Invivogen ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged and dissociated as described above for EB generation into single cells and cultured on Matrigel-coated plates with the STEMdiff Trilineage differentiation kit (used according to the manufacturer’s instructions, Stemcell Technologies, 05230). Cells were harvested at the indicated day for gene expression analysis by qPCR.
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bioRxiv - Bioengineering 2022Quote: T cells were isolated from human donor whole blood samples by negative selection using the RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies). Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... total B cells from the spleens and lymph nodes were isolated using the Negative Selection B cell isolation kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ T-cells were aseptically isolated from the spleen and lymph nodes of C57BL/6 or FVB (6-8 weeks old) mice using the negative selection Easysep mouse CD8+ T-cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Splenocytes and lung cells were enriched for total B cells using the EasySep Mouse Pan-B Cell Isolation kit (Stemcell Technologies) while whole mln cells were used for downstream processing ...