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Citations for Stemcell Technologies :
901 - 950 of 1509 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... with 5×103 splenic P14 TN cells congenic for CD90.1+ or CD45.1+ isolated by negative selection using the EasySep Mouse CD8+ T cell isolation kit (STEMCELL Technologies) before LCMV Arm infection eight hours later ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19258). All isolated T cells were activated by CD3/CD28 Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... https://vi-talant.org/Home.aspx) by using the negative selection “RosetteSep Human NK Cell Enrichment Cocktail” kit (#15065; STEMCELL Technologies). Primary NK cells were cultured in CellGenix® GMP stem-cell growth media (SCGM ...
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bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... dendritic cells (DCs) were enriched by magnetic isolation using the EasySepTM Mouse Pan-DC Enrichment Kit (Stem Cell Technologies). DCs were plated into 96-well U-bottom plates (100 × 106 cells/well) ...
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bioRxiv - Neuroscience 2023Quote: ... and Naïve CD4+ T cell were isolated from splenocytes with commercial kits (STEMCELL Technologies, Cat No. 19852, 19853, 19765) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Systems Biology 2023Quote: ... TRA-1-60) as well as differentiation into the three germ layers (STEMdiff Trilineage Differentiation kit, #05230, StemCell Technologies).
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: NK Cells were enriched from freshly isolated PBMC using the EasySep™ Human NK Cell Isolation Kit (STEMCELL Technologies). NK cells were then counted and resuspended at 1M/mL in RPMI-1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... B cells were extracted by negative selection with the EasySep™ Human B Cell Isolation Kit (StemCell Technologies, #17954). EBV-containing supernatants from B95-8 cells were added to purified B cells supported by 30 µg mL-1 holo-transferrin and 2.5 µg mL-1 CpG (ODN2006) ...
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bioRxiv - Immunology 2023Quote: ... one piece (∼3g) was used for CD25+CD8- Treg enrichment using a custom thymic Treg selection kit (STEMCELL Technologies)102 ...
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bioRxiv - Immunology 2023Quote: Human neutrophils were purified from blood of healthy volunteers with the EasySep neutrophil isolation kit (StemCell Technologies, Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cells are cultivated in Epicult basal medium with Supplement C (Epicult-C human media kit, Stem Cell Technologies, Vancouver), 5% FCS ...
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bioRxiv - Bioengineering 2023Quote: All six independent iPSC lines were further differentiated into iMSCs using the STEMdiff TM Mesenchymal Progenitor kit (STEMCELL Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in the required volume of Formation Medium prepared using the STEMdiff Cerebral Organoid Kit (Stem Cell Technologies) supplemented with 50 μM Rho-associated protein kinase (ROCK ...
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bioRxiv - Immunology 2023Quote: B cells were isolated from naïve mouse spleens with EasySepTM Mouse Pan-B cell Isolation Kit (Stem Cell Technologies) and suspended in Opti-MEM™ I Reduced Serum Medium (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T lymphocytes were negatively selected for using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies #19851) and cultured in RPMI containing L-glutamine (Corning # MT10040CM ...
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bioRxiv - Immunology 2023Quote: ... using the EasySep™ Direct Human Neutrophil Isolation Kit according to the manufacturer’s instructions (StemCell Tech, Vancouver, BC, Canada). Each sorting resulted in more than 90% neutrophils ...
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bioRxiv - Immunology 2023Quote: Total monocytes were isolated by negative selection using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (StemCell Technologies) using the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and lineage negative cell fraction were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies #19856). Cell sorting of LSK or LT-HSC ...
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bioRxiv - Neuroscience 2023Quote: Differentiation of iPSCs into iPSC-derived hematopoietic progenitor cells (iHPCs) was carried out using STEMdiffTM Hematopoietic kit (STEMCELL Technologies) as previously described (4 ...
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bioRxiv - Immunology 2023Quote: B cells were isolated from naïve mouse spleens with EasySepTM Mouse Pan-B cell Isolation Kit (Stem Cell Technologies) and stimulated following the same method as BCR signal analyses described above ...
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bioRxiv - Microbiology 2024Quote: Both wild type (WT) and RFP labeled iPSCs were differentiated to HPCs using STEMdiff hematopoietic kit (Stem Cell Technologies). Briefly ...
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 T cells were isolated separately using EasySep Human CD4+ or CD8+ Enrichment Kits (Stem Cell Technologies). Isolated T cells were immediately activated with DynaBeads Human T-Activator CD3/CD28 (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... followed by depletion of non-NK lineages using immuno-magnetic beads (EasySep human NK cell enrichment kit, Stemcell Technologies). Samples were sequenced in dedicated flow cells on a HiSeq 2500 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... after which bulk T cells T cells were enriched using EasySep Human T Cell Enrichment Kits (Stem Cell Technologies), then maintained in RPMI-1640 (ATCC ...
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bioRxiv - Neuroscience 2024Quote: ... medium was replaced with STEMdiff™ Hematopoietic Supplement B medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). Cells remained in this medium for 7 additional days ...
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bioRxiv - Bioengineering 2024Quote: ... (i) Dextran-coated MNPs and anti-CD4 antibodies (EasySep Human CD4 Positive Selection Kit II, STEMCELL Technologies Canada Inc.) were incubated at a 1:1 ratio over night at 3 – 8 °C ...
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bioRxiv - Physiology 2024Quote: Bone marrow neutrophils were isolated using the EasySep Mouse Neutrophil Enrichment Kit according to the manufacturer’s instructions (STEMCELL Technologies). Isolated neutrophils were then resuspended in HBSS buffer [containing 0.1% of glucose ...
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bioRxiv - Immunology 2024Quote: Bone-marrow monocytes were purified from bone marrow cellular suspensions using an EasySepTM Mouse Monocyte Isolation Kit (STEMCELL Technologies), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... T cells were obtained using a CD3+ T cell enrichment kit (EasySep™ Human T Cell enrichment, Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Cells were subjected to a 20-day protocol for hepatocyte differentiation using the STEMdiffTM hepatocyte kit (Stem Cell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... EBs were first differentiated into hematopoietic progenitor cells (HPCs) using the STEM diff Hematopoietic Kit (Cat#05310; STEMCELL Technologies). Following a previously established protocol ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes were then fused to Sp2/10-Ag14 cells with polyethylene glycol using the ClonaCell Hybridoma kit (StemCell Technologies). Fused cells were cultured overnight in T-75 flasks to recover ...
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bioRxiv - Immunology 2024Quote: ... Human neutrophils were isolated using the EasySepTM Direct Human Neutrophil Isolation Kit (STEMCELL technologies Canada Inc., Vancouver, BC, Canada), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were then enriched from the PBMC samples via the EasySep Human Monocyte Enrichment Kit (StemCell Technologies Inc, #19059). Monocytes were seeded at a concentration of 500.000 cells/mL in blood cell growth media (Sigma-Aldrich ...
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A non-invasive method to sample immune cells in the lower female genital tract using menstrual discsbioRxiv - Immunology 2024Quote: ... CVF was enriched for CD45+ cells using the EasySep™ Release Human CD45 Positive Selection Kit (Stem Cell Technologies) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... FA000010 (RUCDR/Infinity BiologyX) iPSCs were differentiated into hematopoietic precursors cells (HPCs) using the STEMdiff Hematopoietic kit (STEMCELL Technologies) largely by manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Bone marrow plasma cells (BMPCs) were further enriched using EasySep™ Release Mouse CD138 Positive Selection Kit (StemCell Technologies) and cryopreserved in 10% DMSO in FBS ...
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bioRxiv - Bioengineering 2024Quote: ... CD14+ human monocytes were isolated from the PBMCs using an EasySep™ Human Monocyte Enrichment Kit (19058, StemCell Technologies). The isolated monocytes were cultured in RPMI medium supplemented with 10% FBS and 1% Antibiotic-Antimycotic (A-A ...
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bioRxiv - Immunology 2024Quote: ... Neutrophils were isolated using the Ly6G-PE positive selection kit according to the manufacturer’s instructions (STEMCELL technologies, Catalog#17666). Degranulation assay was performed based on (Lorenzo-Herrero et al. ...
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bioRxiv - Immunology 2024Quote: ... T cells were purified by negative immunomagnetic selection using an EasySep T-cell enrichment kit (StemCell Technologies, Grenoble, France) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... HPCs were diluted to 50,000 cells per well in 100 µL of Media B (STEMdiff Hematopoietic Kit, STEMCELL Technologies) in a 96-well plate ...
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bioRxiv - Cell Biology 2024Quote: Cerebral organoids with telencephalic identity cells were generated as described previously using STEMdiff Cerebral Organoid Kit (StemCell Technologies, 08570), Figure 1A Lancaster et al ...
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bioRxiv - Cancer Biology 2024Quote: ... Sameer Agnihotri (University of Pittsburg) and grown in neurobasal media with complete NeuroCult Proliferation kit (Stem Cell Technologies, #05702), supplemented with EGF ...
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bioRxiv - Cancer Biology 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights EasySep Magnets (StemCell Tech.) according to the manufacturer protocol ...