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Citations for Stemcell Technologies :
1201 - 1250 of 1509 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
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bioRxiv - Immunology 2022Quote: ... NK cells were either isolated from cryopreserved PBMCs by either negative selection using the EasySep NK cell enrichment kit (StemCell technologies) or by depletion of T cells with human CD3 MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells from spleens were negatively enriched using an EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853) according to the manufacturer’s instructions (STEMCELL Technologies). Enriched CD8+ T cells (92% - 96% purity ...
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow plasma cells (BMPC) were enriched from bone marrow mononuclear cells using the EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% DMSO in FBS.
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bioRxiv - Molecular Biology 2022Quote: ... Neutrophils were isolated from 20 ml of blood with EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, British Columbia, Canada, 19666) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... apoptotic and dead cells were removed using immunomagnetic cell separation with the Annexin Dead Cell Removal Kit (StemCell Technologies, Cat# 17899) and EasySepTM Magnet (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated from spleens and lymph nodes of P14 Tcf7GFP or OTI Tcf7GFP mice using the EasySepTM Mouse CD8+ T cell Isolation Kit (Stemcell Technologies), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Splenic naïve CD4+ T cells were negatively selected using an EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naïve T cells were resuspended in supplemented RPMI 1640 medium with penicillin (100 U/ml) ...
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Immunology 2022Quote: ... or GZB-cre Zbtb20-fl/fl CD45.1 OT-I mice (KO) and naive CD8 T cells purified using EasySep Mouse Naive CD8 T Cell Isolation Kits (catalog no. 19858A; STEMCELL Technologies). Fifty thousand naive OT-I cells were retro-orbitally injected into congenic B6 recipient mice ...
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bioRxiv - Microbiology 2022Quote: ... Loosely adherent immature BMDCs were collected and then CD11c+ DCs were enriched using CD11c positive selection kit (Stemcell Technologies, Cambridge, MA). Twenty-four hours after culture of purified subsets ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... primary CD4+ T-cells were negatively enriched using EasySep Human CD4+ T-cell isolation kit (Catalog no. 17952; Stem Cell Technologies) and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2 ...
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bioRxiv - Immunology 2023Quote: ... and purified CD4+ T cells were obtained by positive selection with the “EasySep Mouse CD4 Positive Selection II” kit (StemCell Technologies). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary human CD3+ T cells were isolated from PBMCs by negative selection using EasySep Human T Cell Isolation Kit (StemCell Technologies) or by positive selection using Dynabeads Human T-Expander CD3/CD28 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse T-cells were isolated from mouse PP/lymph nodes/spleens and tumors using EasySep Mouse CD8+ T Cell Isolation kit (StemCell Technologies). For FACS analysis ...
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bioRxiv - Genetics 2023Quote: ... Common karyotypic abnormalities were measured using the qPCR-based hPSC Genetic Analysis Kit following manufacturer’s instructions (STEMCELL Technologies; cat. no. 07550). Cell lines with true karyotypic abnormalities were not used in this study.
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bioRxiv - Genomics 2023Quote: Human CD8+ T cells were obtained from either pooled PBMC donors (ZenBio) using negative selection human CD8 isolation kits (StemCell Technologies) or directly from vials containing isolated CD8+ T cells from individual donors (StemCell Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Immunology 2024Quote: ... OT-I+/- or total CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection using the EasySepTM Mouse CD8+ T cell isolation kit (#19753, StemCell Technologies). For in vitro experiments ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were isolated from PBMCs by CD14 positive selection using the EasySep™ Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ CD45RO+ memory T cells were then isolated by negative selection using an EasySepTM Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies).
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bioRxiv - Cancer Biology 2024Quote: NK cells were isolated from PBMCs of healthy donors by depleting CD3 cells using an EasySep human CD3 positive selection kit II (StemCell Technologies). The negative cell fraction was expanded using irradiated (100 Gy ...
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bioRxiv - Immunology 2024Quote: ... The resulting hybridoma cells were selected on semi-solid methylcellulose and grown in 96-well plates using the ClonaCell-HY Hybridoma Cloning Kit (Stemcell Technologies). Hybridomas secreting SH-01-reactive IgG were identified by several rounds of ELISA screening ...
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were then isolated from the PBMC fraction using the EasySep™ Human CD14 Positive Selection Kit II (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... mTESR plus medium was replaced with STEMdiff™ Hematopoietic Supplement A medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). On day 3 ...
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bioRxiv - Molecular Biology 2024Quote: ... femur and tibia were flushed and neutrophils (PMN) enrichment was performed with the EasySep mouse neutrophil enrichment kit (Stem Cell Technologies). PMN ROS production upon stimulation with PMA (100 nM ...
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bioRxiv - Neuroscience 2024Quote: hiPSC derived neural progenitor cells (NPCs) were generated using the embryoid body protocol and STEMdiff SMADi Neural Induction Kit (STEMCELL Technologies). From these NPCs ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells (OT-I cells) were isolated by negative selection with the EasySepTM Mouse CD8 T cell isolation kit (Catalog #19853, Stemcell Technologies) following the manufacturers protocol ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were first enriched for memory B-cells using the EasySep Human Memory B Cell Isolation Kit (Stemcell Technologies, catalog #17864) and sequenced using the 10x Genomics pipeline as described by Hurtado et al.37 Sequencing data were analyzed using Cell Ranger and custom Python scripts.
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bioRxiv - Immunology 2024Quote: ... Cells were filtered through a 70 µm nylon strainer and further purified with the EasySepTM mouse neutrophil enrichment kit (StemCell Technologies), following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... EB-HSPCs, and primary mPBSCs from a healthy donor (female, 30 years) were differentiated using the StemDiff T Cell Kit (STEMCELL Technologies) with minor modifications to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T lymphocytes were isolated from PBMCs by positive selection using magnetic beads (EasySep Human CD4 positive selection kit II, StemCell Technologies) and activated using ImmunoCult human CD3/CD28 T cell activator cocktail (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T were isolated from the spleen and LN of OT-II TCR Tg GFP+ mice using the Easysep Mouse CD4+ T cell isolation kit (Stemcell Technologies) or MojoSort Mouse CD4 Naïve T cell isolation kit (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... γδ TCR+ cells were enriched by immunomagnetic negative selection using an EasySep Human Gamma/Delta T Cell Isolation Kit (STEMCELL Technologies). After purification ...
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bioRxiv - Immunology 2024Quote: We isolated CD8+ T cells from primary human donor PBMCs using the EasySep Human CD8+ T cell Isolation Kit (STEMCELL Technologies) and activated them overnight using anti-CD3/CD28 DynaBeads (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... with minor modifications.18 CD34+ cells were isolated directly from PBMCs or from enriched HSPCs (RosetteSep Hematopoietic Progenitor Enrichment Cocktail Kit, Stemcell Technologies) via immunomagnetic isolation (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... CD3 negative selection was performed utilizing the EasySep™ Human APC Positive Selection Kit II (Stem Cell Technologies, Cat-no. 17661) with CD3 APC antibody (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were plated at density of 65,000 cells per well in Neural Induction Media (STEMdiff SMADi Neural Induction Kit STEMCell Technologies, 08581) in a 96-well v-bottom plate to form neural aggregates ...
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bioRxiv - Cancer Biology 2024Quote: Primary mouse NK cells were isolated from spleens of 8-12-week-old wild-type or Prf1-/- mice using the negative selection EasySep mouse NK cell isolation kit (STEMCELL Technologies) or positive selection CD49b (DX5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then harvested and stained using the Annexin V Apoptosis Detection Kit with 7-AAD (STEMCELL Technologies, Vancouver, BC, Canada), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: CD4+ T-cells were isolated from frozen PBMCs from 5 study participants using the EasySep Human CD4+ T-Cell Enrichment Kit (Stemcell Technologies). 2 million CD4+ T-cells were then cultured in 96-well plates in duplicate in 200 μL R10+ media supplemented with 100 U/mL IL-2 ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the mouse spleen and injected with mEERL cells with scramble or Marchf8 sgRNA using the CD8+ T-cell Isolation Kit (STEMCELL Technologies). The cells were labeled with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: Splenic B cells from BACtg and wild type littermate control mice were isolated using the EasySep Mouse B Cell Isolation Kit (StemCell Technologies) and rested for 30 minutes and then either left unstimulated or stimulated with the addition of 10 μg/ml goat anti-IgM F(ab’)2 crosslinking antibody (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2024Quote: RNA extraction and quantitative PCR: Tumor infiltrating myeloid cells were isolated from tumors either using a CD11b isolation kit (catalog # 18970, StemCell Technologies) or by cell sorting ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the spleens of C57BL/6J or mice with magnetic bead-based CD8 T cell isolation kit (Cat # 19853, StemCell Technologies). T cells from naïve mice were stained with 5µM Cell Trace Violet (Cat# C34557 ...
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bioRxiv - Bioengineering 2024Quote: ... CD8+/CD4+T cells (for OT-I/OT-II mice) were isolated from this suspension using the EasySep Mouse CD8+/CD4+ T Cell Isolation kit (STEMCELL Technologies; Cat#19852 for CD4+T ...