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Citations for Stemcell Technologies :
8751 - 8800 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... CD4+ T cells were enriched by negative selection using the EasySep Human CD4+ T Cell Enrichment Kit (StemCell Technologies). The purity of the CD4+ T cells was evaluated by flow cytometry and was higher than 95% ...
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bioRxiv - Genetics 2019Quote: ... Naive and memory CD4+ T cells were isolated from PBMCs using EasySep® naive CD4+ T cell isolation kit and memory CD4+ T cell enrichment kit (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... peripheral blood mononuclear cells (PBMCs) were processed from Leukopak (STEMCELL Technologies, Vancouver, BC, Canada). Monocytes were isolated from PBMCs using the EasySep Human Monocyte Enrichment Kit (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Monocytes were isolated from PBMCs using the EasySep Human Monocyte Enrichment Kit (STEMCELL Technologies, 19058) and were differentiated in RPMI medium (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... Sl/Sl stromal cells (Stem Cell Technology), M2-10B4 stromal cells (Stem Cell Technology) ...
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bioRxiv - Molecular Biology 2019Quote: ... M2-10B4 stromal cells (Stem Cell Technology), and CD34+ HPCs were maintained as previously described (18) ...
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bioRxiv - Cancer Biology 2019Quote: Tissue samples were minced and digested with collagenase/hyaluronidase (Stemcell technologies) in complete Epicult-B Medium supplemented with 0.48 µg/mL hydrocortisone (Stemcell technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... in complete Epicult-B Medium supplemented with 0.48 µg/mL hydrocortisone (Stemcell technologies) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... which were bought from STEMCELL Technologies (Cambridge, MA #70025.1). Normal human fibroblasts and breast epithelial cells were obtained from Coriell Institute (NJ ...
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bioRxiv - Immunology 2019Quote: ... The Jackson Laboratory) using a negative selection kit (StemCell Technologies, # 19854).
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bioRxiv - Developmental Biology 2019Quote: ... embryos were treated with Collagenase Type I (Stemcell technologies; 07902) at 0,125% for 1h at 37º ...
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bioRxiv - Molecular Biology 2019Quote: ... PneumaCult-ALI Medium was made up as per manufacturer’s recommendations (Stemcell Technologies, 05001).
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bioRxiv - Developmental Biology 2019Quote: ... 5% CO2 and ambient oxygen level on Geltrex coated plates in mTeSR1 medium (STEMCELL Technologies, 05850) with daily medium change ...
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bioRxiv - Immunology 2019Quote: ... clone IV.3 (STEMCELL Technologies; 60012FI); Brilliant Violet 711 conjugated anti-human FcγRIII ...
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bioRxiv - Bioengineering 2019Quote: ... Undifferentiated hESCs were cultured in mTeSR1 (StemCell Technologies; 05850) in Accutase (StemCell Technologies ...
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bioRxiv - Bioengineering 2019Quote: ... in Accutase (StemCell Technologies; 07920) and passaged every 3 d.
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bioRxiv - Developmental Biology 2019Quote: ... in mTesr medium (Stem cell technologies), supplied with 50μM Rho-associated kinase (ROCK ...
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bioRxiv - Neuroscience 2019Quote: ... media was supplemented with Y27632, Laminin (2.5 µg per 1ml of media, Thermo-Fisher) and 0.1µM Compound-E (Stem Cell Technologies, Grenoble, France) during the first 3 days after plating in Poly-L-Lysine (Sigma ...
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bioRxiv - Immunology 2019Quote: Leukapheresis was performed on three normal donors using Institutional Review Board (IRB)-approved consent forms and protocols by StemCell Technologies (Vancouver, BC). Approximately two to three blood volumes were processed using the Spectra Optia® Apheresis System to produce a full-sized Leuko Pak ...
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bioRxiv - Immunology 2019Quote: ... The sample was resuspended in growth media and B cells were negatively selected using the EasySep Human B cell Isolation Kit according to the manufacturer’s protocol (STEMCELL Technologies, Vancouver, BC). Because our samples contained greater than one million PBMCs ...
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bioRxiv - Cell Biology 2019Quote: ... incubated for 2 min in NH4Cl red blood cell lysis solution (StemCell Technologies, cat. # 07800, Vancouver, Canada), and seeded into culture plates in αMEM supplemented with 15% FBS and antibiotics ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using the EasySep Biotin Positive Selection Kit (Stemcell Technologies) with anti-human/mouse β7 integrin antibody (FIB504 ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1% of 0.2% heparin (StemCell Technologies), 20ng/mL EGF/bFGF and 10ng/mL PDGF-AA/PDGF-BB (all from Shenandoah Biotechnology) ...
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bioRxiv - Biochemistry 2019Quote: ... suspension cultures were mechanically disaggregated and filtered through 37 µm filters (StemCell Technologies, Vancouver, Canada) in order to obtain bona fide single-cell suspension ...
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bioRxiv - Immunology 2019Quote: ... B cells were isolated using a negative selection B cell isolation kit (Stemcell Technologies, #19854A). The A20 murine IgG+B-cell line was obtained from ATCC (#TIB-208) ...
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bioRxiv - Immunology 2019Quote: Human T-cell subsets were isolated from leukapheresis produts using negative selection kits from STEMCELL Technologies. Rosette Sep (Stemcell Technologies ...
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bioRxiv - Immunology 2019Quote: ... Rosette Sep (Stemcell Technologies) was used to enrich for total CD8+ or CD4+ T-cells ...
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bioRxiv - Immunology 2019Quote: ... Naïve and memory cells were isolated using EasySep Enrichment kits (negative selection, Stemcell Technologies). Flow cytometric analysis based on CD62L and CD45RAwas used to confirm purity is above >92% ...
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bioRxiv - Cancer Biology 2019Quote: Single dissociated cells were suspended in Aldefluor assay buffer (STEMCELL technologies, Cambridge UK) and incubated with an ALDH substrate ...
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bioRxiv - Immunology 2019Quote: OT2 T cells were isolated by magnetic beads (EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit, StemCell Technologies) from a single cell suspension of splenocytes ...
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bioRxiv - Immunology 2019Quote: ... or EasySep™ mouse neutrophil enrichment kit (StemCell technologies) as previously described (Park et al. ...
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bioRxiv - Bioengineering 2019Quote: ... Conditioned cells were split with Accutase (STEMCELL Technologies, Köln, Germany) instead of Trypsin ...
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bioRxiv - Neuroscience 2019Quote: ... and passaged once every 5-6 days using Dispase (StemCell Technology). To maintain pluripotency and limit spontaneous differentiation ...
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bioRxiv - Microbiology 2019Quote: ... freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: Freshly isolated PBMCs obtained from healthy donor peripheral blood (lymphocyte separation media; StemCell Technologies) were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend) ...
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bioRxiv - Genetics 2019Quote: ... in mTESR-1 medium (StemCell Technologies) [20] ...
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bioRxiv - Immunology 2019Quote: ... and Immunocult anti-CD3/28 activator (Stemcell Technologies; 10971) for 4 days ...
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bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: One mM of EDTA and 50 µL/mL RosetteSep human monocyte enrichment cocktail (STEMCELL Technologies, Vancouver, Canada) were added to blood and incubated for 20 minutes at room temperature before dilution with an equal volume of PBS enriched with 2% FCS ...
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bioRxiv - Cell Biology 2019Quote: ... Routine passaging every 4-5 days was performed using ReLeSR (Cat. # 05873; STEMCELL Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... spheroid cultures were dissociated using Accutase (StemCell Technologies) when they had reached diameters of ∼100 µm ...
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bioRxiv - Cancer Biology 2019Quote: ... iPSCs were maintained in mTeSR1 medium (StemCell Technologies) on tissue culture plates coated with Matrigel (Corning ...
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bioRxiv - Neuroscience 2019Quote: Undifferentiated pluripotent hESC were cultured in mTeSR1 media (Stem Cell Technology) on Matrigel-coated plates with daily media changes and passaged using L7 passaging media (Lonza ...
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bioRxiv - Neuroscience 2019Quote: ... or ReLeSR passaging media (Stem Cell Technology). The anti-ATXN3 ASO and scrambled control ASO (ASO-Ctrl ...
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bioRxiv - Cancer Biology 2019Quote: ... or Robosep (STEMCELL Technologies, Vancouver, Canada). DNA from peripheral blood was used as a control sample for each patient to exclude germline variants ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.1 mg/ml DNase (Stem Cell Technologies 07900), and filtered through a 40 µm cell strainer ...
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bioRxiv - Microbiology 2019Quote: CD34+ HPCs isolated from human bone marrow were infected at an MOI of 2 for 20h in IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies, Canada), 2 mM L-Glutamine ...
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bioRxiv - Microbiology 2019Quote: ... Canada) M2-10B4 and Sl/Sl stromal cell monolayer [58] for 10-12 days in Myelocults (Stem Cell Technologies) containing 1 µM hydrocortisone and penicillin/streptomycin ...
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bioRxiv - Microbiology 2019Quote: ... Pure populations of sorted HPCs were plated either at 500 cells/mL in Methocult H4434 (Stem Cell Technologies) in 6 well plates in triplicate for myeloid colony assays ...
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bioRxiv - Bioengineering 2019Quote: The maintenance of hiPSCs was done using iMatrix-511 (Matrixome) in mTeSR1 medium (STEMCELL Technologies). Culture medium was changed every other day ...