Labshake search
Citations for Stemcell Technologies :
751 - 800 of 4935 citations for Rat Activated Leukocyte Cell Adhesion Molecule CD166 ALCAM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: CD4+ and CD8+ T cell subsets were depleted from PBMC using magnetic bead-based CD4 and CD8 negative selection kits (Stem Cell Technologies, Vancouver, Canada). The cell separations were performed according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
-
bioRxiv - Immunology 2020Quote: ... Each antibody clone and lot was titrated to optimal staining concentrations using primary murine samples with all appropriate positive and negative controls: polyclonal murine CD8 T cells purified by positive selection kit (Stem Cell Technologies, Vancouver, Canada) stimulated with PMA/Ionomycin via eBioscience Cell Stimulation Cocktail (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... B cells were enriched by negative selection using EasySep mouse B cell enrichment kit as per manufacturer’s instructions (StemCell Technologies Inc., Tukwila, WA, USA). Enriched B cells were then resuspended in FACS buffer and stained and sorted as described previously(Carbonetti et al. ...
-
bioRxiv - Bioengineering 2022Quote: ... CD4+ T cells were isolated from the spleen of C57/BL6 mice using the CD4 T cell isolation kit from Stemcell Technologies (# 19852; Cologne, Germany) and subsequently naïve T helper cells (CD3+ ...
-
bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
-
bioRxiv - Cancer Biology 2023Quote: An Easy Step Human CD8+ T Cell Isolation Kit was used to isolate CD8+ T cells from a donor PBMC sample via negative selection (Cat #, 17913, Stem Cell Technologies, Vancouver, Canada).
-
bioRxiv - Cancer Biology 2023Quote: ... malignant B cells were enriched from splenocytes of Eµ-TCL1 mice using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, Inc., Cologne, Germany). The CD5+ CD19+ content of purified cells was typically above 95% ...
-
bioRxiv - Immunology 2023Quote: Single cell suspensions of splenocytes were prepared and CD4+ T cells were isolated using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies; Cat # 19852A). CD4+ T cells were plated in a round bottom 96-well plate at 2 × 105 cells/well and activated using Mouse T Activator CD3/CD28 Dynabeads™ (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were isolated from healthy donor-derived PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. # 17953). CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific ...
-
bioRxiv - Immunology 2024Quote: ... Dendritic cells (DCs) were isolated from spleens of wild type mice using EasySep™ Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies, 19863). Co-cultures were set up in 4 replicates with BMDM primed as above ...
-
bioRxiv - Genomics 2019Quote: CD4+ T cells were enriched from PBMCs by negative MACS selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies SARL, Grenoble, France), according to manufacturer’s instructions.
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... splenic T-lymphocytes were negatively selected from total splenocytes through use of EasySep Mouse T-cell Isolation Kit (Stem Cell Technologies #19851, Vancouver, BC, Canada). Samples were validated for >90% viability and purity before subsequent use.
-
bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
-
bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: Astrocyte precursor cells were created from the neural progenitor cells described above using the STEMdiff Astrocyte Differentiation media kit from STEMCELL Technologies (Cat No. 100-0013) following their protocol for Matrigel-coated 6-well plates [30] ...
-
bioRxiv - Cell Biology 2023Quote: Transduced lineage negative (Lin-) or Lin-Sca-1+Kit+ (LSK) cells were plated into 1mL of methylcellulose-based medium with cytokines (Stem Cell Technology, MethoCult GF M3434) into 35 x 10 mm non-adherent Bio-One Petri Dish ...
-
bioRxiv - Bioengineering 2024Quote: ... PBMC samples were thawed on the day of activation and T cells were purified via the EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies Inc, cat#19053). Isolated T cells were seeded in the CD3/CD28 precoated well plates at a concentration of 500,000 cells/mL with 1 mL/well in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies Inc ...
-
bioRxiv - Neuroscience 2024Quote: ... E18 rat hippocampal tissue (SDEHP, Brain Bits) were dissociated with papain (PAP, Brain Bits) and DNase I (07469, Stemcell Technologies) following the manufacturer instructions for neuron dissociation ...
-
bioRxiv - Immunology 2019Quote: ... hMT and s-hMT mice were purified with the EasySep Mouse B Cell Isolation Kit (Stemcell Technologies). B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
-
bioRxiv - Immunology 2021Quote: ... Negative selection of NK was performed on EasySep™ Human NK Cell Enrichment Kit system (StemCell Technologies). 2.106 sorted NK cells were cultured with 100 Gy-irradiated EBV-LCL cells in a 1:20 ratio ...
-
bioRxiv - Immunology 2021Quote: ... CD34+ cells were enriched with the EasySep Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) without using the RosetteSep™ Human Cord Blood CD34 Pre-Enrichment Cocktail ...
-
bioRxiv - Neuroscience 2020Quote: Embryoid bodies (EB) were obtained as described [36] and using tri-lineage differentiation kit (Stem Cell Technologies). Germ layer differentiation was assessed by immunochemistry.
-
bioRxiv - Cancer Biology 2022Quote: ... CD20+ target cells were isolated using the EasySep Human Biotin Positive Selection Kit II (StemCell Technologies, Canada) and the biotinylated anti-CD20 antibody (BioLegend ...
-
bioRxiv - Cell Biology 2022Quote: ... CD15-expressing neutrophils were isolated using the EasySepTM direct neutrophil isolation kit (Stem Cell Technologies, Seattle, WA) within 1 hour of the blood draw as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: RALDH activity in individual cells was analyzed using the aldefluor staining kit (StemCell Technologies, Vancouver, BC, Canada). Briefly ...
-
bioRxiv - Immunology 2021Quote: ... Neutrophils from HDs were purified by the EasySep Human Neutrophil Enrichment Kit (Stem Cell Technologies; > 99.7% purity) after centrifuging buffy coats onto Ficoll-Paque gradient density ...
-
bioRxiv - Microbiology 2021Quote: ... Human CD34+ HSC were isolated using a CD34+ HSC isolation kit (Stem Cell Technologies, Cambridge, MA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... NK cells were then isolated from these PBMCs using a magnetic negative selection kit (STEMCELL Technologies, 17955). Briefly ...
-
bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
-
bioRxiv - Immunology 2019Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... For Il2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
-
bioRxiv - Immunology 2022Quote: Bone-marrow eosinophils were isolated using EasySep™ Mouse Biotin Positive Selection Kit II (Stem Cell Technologies) according to manufacturer instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: MSCs were differentiated into chondrocytes using the MesenCult-ACF Chondrogenic Differentiation Kit (Stem Cell Technologies; Catalogue #05426) comprising of MesenCult-ACF Chondrogenic Differentiation Basal Medium (Stem Cell Technologies ...
-
bioRxiv - Developmental Biology 2024Quote: ... NSC suspensions were seeded at 200,000 cells per well in STEMdiff Neuron Differentiation Kit medium (Stemcell Technologies) and medium replaced every other day until day 7 ...
-
bioRxiv - Immunology 2024Quote: ... Splenocytes or BM cells were enriched using EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies) per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Marie-Thérèse El-Daher derived NPCs using the StemDiff Neural Induction Kit (IM, 08581, Stem Cell Technologies) following the embryoid-body (EB ...
-
bioRxiv - Cancer Biology 2023Quote: ... All sorted cells were grown in Mouse MesenCult Expansion Kit Media (05513; StemCell Technologies, Vancouver, BC, Canada). Cells were cultured at 37 °C with 5% CO2 ...
-
bioRxiv - Immunology 2023Quote: ... Lymph node CD8α+ T cells were isolated and sorted using the CD8α Positive Selection Kit (Stemcell Technologies). Isolated cells were washed and resuspended at 1 x 106 cells/ml in stimulation media ...
-
bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2023Quote: iPSCs were differentiated to NPCs using the STEMdiff™ SMADi Neural Induciton Kit (Stem Cell Technologies, 08581) according to manufacturer instructions ...
-
bioRxiv - Immunology 2024Quote: EasySep Mouse Pan-B cell Isolation and EasySep Release Mouse CD138 Positive Selection kits from STEMCELL Technologies were used to enrich B cells and ASCs from ∼5x107 SPL cells following manufacture guidelines ...
-
bioRxiv - Cancer Biology 2024Quote: The ALDH enzyme activity in human melanoma cells was measured using the Aldefluor assay kit (StemCell Technologies) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... CD8+ T-cells were negatively isolated from PBMC by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies; average purity 93%, n=16).
-
bioRxiv - Cancer Biology 2023Quote: ... T cells were then isolated using EasySep™ Human T Cell Isolation Kit and EasySep™ Magnet (Stemcell Technologies, Cat. No. 17951 and Cat. No. 18000) following manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
-
bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
-
bioRxiv - Cancer Biology 2020Quote: The aldehyde dehydrogenase (ALDH) activity was detected using an ALDEFLUOR assay kit (Stem Cell Technologies, Canada, Cat #1700) according to the manufacturer’s protocol ...