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Citations for Stemcell Technologies :
651 - 700 of 4935 citations for Rat Activated Leukocyte Cell Adhesion Molecule CD166 ALCAM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Primary human NK cells were isolated from PBMCs from three donors by negative selection using the EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies 17955), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 100,000-200,000 CD4+ T cells per well were plated in 96-well V-bottom plates and rested for 1.5 hours at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 250,000-500,000 CD4+ T cells per well were plated in 96-well V-bottom plates ...
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bioRxiv - Cell Biology 2020Quote: ... cells were patterned into definitive endoderm using the StemDiff Definitive Endoderm kit (STEMCELL Technologies, #05110). On day 3 ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies). Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... or EasySep™ Release Mouse PE Positive Selection Kit (Stem Cell Technologies, Cat no. 17656) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Viability enrichment was performed using the EasySep Dead Cell Removal (Annexin V) Kit (StemCell Technologies) as per manufacturers protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... human leukemia cells were isolated using EasySepTM Mouse/Human Chimera Isolation Kit (#19849; Stemcell Technologies). All animal experiments were approved by and performed in accordance with guidelines of the Institutional Animal Care and Use Committee at University of Colorado (protocol No ...
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bioRxiv - Genomics 2022Quote: ... H1 cells were differentiated to germ layers using the STEMDiff Trilineage Differentiation Kit (STEMCELL Technologies) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2019Quote: ... NK-cells were enriched from PBMC using a CD56-positive selection kit (Stemcell Technologies, Canada). NK cells were expanded using the Amplification and Expansion System (NKAES ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies). Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... enrichment of CD8+ T cells was performed using magnetic bead separation kits EasySep (STEMCELL Technologies) or Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... the media was switched to Neural Maturation Medium (STEMdiff Cerebral organoid Kit, STEM CELL Technologies) and media was exchanged every 3 days.
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bioRxiv - Molecular Biology 2021Quote: ... differentiation of hESCs cardiomyocytes was performed using the STEMdiffTM Cardiomyocyte Differentiation Kit (Stem Cell Technology), whereas analysis of cardiomyocyte induction efficiency was conducted via FACS using the cTnT+ primary antibody (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: Cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, STEMCELL Technologies). Purified NK cells were then cultured in stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated using the CD4+ Negative Selection Kit (StemCell Technologies, Cat# 19752) followed by fluorescence-activated cell sorting ...
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bioRxiv - Immunology 2021Quote: ... CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies, Vancouver, Canada) per manufacturer’s instructions and confirmed >90% pure by flow cytometry ...
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bioRxiv - Cancer Biology 2021Quote: ... human CD41+ cells were isolated using the CD41+ selection TAC kit (Cat# 10450, StemCell Technologies).
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bioRxiv - Immunology 2022Quote: ... NK cells were removed from cultures (EasySep Human CD56 Positive Selection Kit II, StemCell Technologies). Remaining CD4 T cells were analyzed by flow cytometry to confirm removal of NK cells by staining for CD56 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated from PBMCs using the EasySep positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were then ultra-purified using an EasySep Direct Neutrophil isolation kit (Stem Cell Technologies) according to the manufacturer’s instructions and subsequently stained with Cell Trace Calcein Red-Orange cell stain (ThermoFisher ...
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Autologous humanized PDX modeling for immuno-oncology recapitulates the human tumor microenvironmentbioRxiv - Cancer Biology 2022Quote: ... CD34+ cells were positively selected using the EasySep Human CD34 Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 2042cl9 and 2042cl5 hiPSCs was performed using STEMdiffTM Trilineage Differentiation Kit (#05230, Stem Cell Technologies) according to manufacturer’s instructions.These experiments were conducted with at least one or two replicates (Fig ...
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bioRxiv - Immunology 2024Quote: ... magnetically isolated splenic P14 CD45.1+ TN (EasySep Mouse CD8+ T cell isolation kit, STEMCELL Technologies) were labeled with CellTrace Violet (CTV ...
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bioRxiv - Cancer Biology 2024Quote: ... coated tissue plates in NeuroCult NS-A proliferation kit media (Stem Cell Technologies Cat. #05751) supplemented with 1% penicillin/streptomycin solution (Sigma Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Neural induction was achieved using the StemDiff Neural Induction Kit (IM, 08581, Stem Cell Technologies) and following the embryoid-body (EB ...
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bioRxiv - Genetics 2023Quote: ... and the cells were further matured using STEMdiff Forebrain Neuron Maturation Kit (STEMCELL Technologies; #08605). Neurons were maintained in maturation media for 25-35 days ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were differentiated using mouse MesenCult™ Adipogenic and Osteogenic Differentiation Kits from StemCell Technologies. Cells were differentiated for 3 weeks and then fixed ...
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bioRxiv - Cancer Biology 2023Quote: ... EasySep™ human CD34 positive selection kit II was procured from Stem cell technologies (17856). Ficoll® Paque PLUS density gradient media was purchased from Sigma Aldrich (GE17-1440-03).
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bioRxiv - Bioengineering 2023Quote: ... and mesodermal lineage cells in vitro using the STEMdiff™ Trilineage Differentiation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Later differentiations also utilized the kit developed based on this protocol (Stem Cell Technologies, 08620) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Pluripotency tests of iPSC lines using the STEMdiffTM Trilineage Differentiation Kit (Stem Cell Technologies, 05230) according to manufacturer instructions demonstrated differentiation capacity of all three germ layers (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampi were dissected from embryonic day 18 (E18) rats and resuspended in BrainPhys medium (BPNM, STEMCELL Technologies, 05790) supplemented with 2% SM1 (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... all the unwanted cells were labelled with magnet conjugated antibody cocktail which is separated using their EasySep magnetic stand leaving CD4 or CD8 cell in suspension using the vendor supplied EasySep Human CD4/CD8 T Cell Iso Kit (Stem Cell Technologies, 17952 for CD4 and 17953 for CD8). This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were first enriched for CD8 T cells using EasySep™ human total or naïve CD8 T Cell enrichment kits (STEMCELL Technologies, Vancouver, Canada). CD56− and CD56+ PBMC fractions were sorted by flow cytometry ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Immunology 2022Quote: ... Splenic T cells were enriched using a magnetic-based commercial CD8 T cell negative selection kit (StemCell Technologies, BC, Canada; Supplemental Figure 3A) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Cell Biology 2023Quote: ... The middle layer containing PBMCs were then enriched for primary human NK cells (hNK) by negative selection using the EasySep Human NK Cell Enrichment Kit (StemCell Technologies, Cat# 19055), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... A crude live cell mixture was obtained by filtration and negative selection using Easy Eights EasySep Magnet and Dead Cell Removal kit (Stemcell Technologies, Catalog # 17899). Cells were further en-riched with positive CD90.2 selection (Stemcell Technologies ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...