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Citations for Stemcell Technologies :
1001 - 1050 of 4935 citations for Rat Activated Leukocyte Cell Adhesion Molecule CD166 ALCAM ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... from wild-type or Jak1 floxed C57/Bl6 mice were isolated using EasySep mouse hematopoietic progenitor cell isolation kit (Stemcell technologies, Vancouver, Canada) which remove lineage marker-expressing cells with biotinylated antibodies directed against non-hematopoietic stem cells and non-progenitor cells (CD5 ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... following a monolayer culture method with a commercial dual SMAD inhibition-mediated neural induction medium (STEMdiff™ SMADi Neural Induction Kit, Stem Cell Technologies) based on a published protocol59 ...
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from spleen and peripheral lymph nodes from indicated mice by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies Inc). 1.75 x 105 cells were cultured for 4 days in RPMI1640 supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Immunology 2020Quote: ... we performed negative B cell enrichment from 100M PBMCs in each experiment using a commercially available B cell enrichment kit according to manufacturer’s protocol (StemCell Technologies; Vancouver, BC, Canada).
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bioRxiv - Bioengineering 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Immunology 2023Quote: ... Cells were filtered through a 70 μm nylon strainer and further purified with the EasySep mouse neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: PP cells generated using the STEMdiff™ Pancreatic Progenitor Kit and expanded under CINI conditions were dissociated into single cells using AccutaseTM (STEMCELL Technologies, 07920) at 37 °C and then resuspended in PEP medium (Table S4 ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were maintained in standard tissue-culture treated 24-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276) and 1x Penn/Strep from a 100x stock (Mediatech ...
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bioRxiv - Bioengineering 2024Quote: The primary T cells were obtained from peripheral venous blood of a healthy human donor using the EasySep™ Direct Human T cell Isolation Kit (STEMCELL TECHNOLOGIES, #19661) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: ... The lungs were collected for single cell suspension and ILC enrichment (by Mouse Pan-ILC Enrichment Kit; STEMCELL Technologies, Vancouver, British Columbia, Canada). The dose of Alt was 30 µg/mouse ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged using Gentle Cell Dissociation Reagent (Stem Cell Technologies, #7174). iPSCs were confirmed to be karyotype normal and maintained below passage 10 and all the cell lines were routinely checked and were negative for mycoplasma.
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bioRxiv - Cell Biology 2021Quote: ... cells were dissociated into single cells using AccutaseTM (Stem Cell Technologies, #07920). 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were passaged using Gentle Cell Dissociation Reagent (Stem Cell Technologies 07174).
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bioRxiv - Neuroscience 2024Quote: ... stem cells were dissociated to single cells by accutase (Stem Cell Technologies) and two days later the differentiation media (DMEM:F12/Neurobasal 1:1 ...
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bioRxiv - Genetics 2019Quote: ... CD4+ T cells fraction of the PBMCs was obtained by negative selection using EasySep® Human CD4+ T Cell Enrichment Kit (Cat. no. 19052, StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: OT1 CD8+ T cells from Wild type or the knockout mice mentioned above were purified from spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853, STEMCELL Technologies, Vancouver, CA). Isolated CD90.1+ OT1 CD8+ T cells were injected into CD90.2+ C57BL6 recipient mice at a concentration of 106 cells/100ul PBS through the retro-orbital route.
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bioRxiv - Cell Biology 2022Quote: We isolated CD34+ cells from human cord blood and BM samples using an EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, Canada); sample purity of >95% was confirmed using flow cytometry ...
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bioRxiv - Immunology 2020Quote: CD4+ OT-II cells were isolated from spleen and LNs from OT-II*B6SJL mice with the EasySep mouse CD4+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... We confirmed our iPSC cell banks have normal copy number at various mutation hotspots using the hPSC Genetic Analysis Kit (Cat # 07550, STEMCELL Technologies, Vancouver, BC, CAN).
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bioRxiv - Cell Biology 2020Quote: Depletion of lineage-committed cells from single-cell suspensions of mouse bone marrow was performed using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, BC, Canada).
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bioRxiv - Immunology 2021Quote: ... Cells from all mice were pooled by tissue type and B cells were selected from lymph node and spleen using a mouse Pan-B negative selection kit (Stemcell Technologies, Vancouver, BC, Canada). Generation of natively paired heavy and light chain libraries ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated by magnetic bead negative selection using EasySep™ Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Mouse B cells were isolated from pooled single cell suspensions of spleen and peripheral lymph nodes using CD19 microbeads (Miltenyi, catalog no. 130-052-201) or EasySep Mouse B Cell Isolation Kit (Stemcell Technologies, catalog no. 19854). B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Neural progenitor cells (NPCs) were created from the hESCs described above using the STEMdiff SMADi Neural Induction kit from STEMCELL Technologies (Cat No. 08581) according to their guidelines for Matrigel-coated 6-well plates [31] ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were differentiated and matured using the StemCell Midbrain Neuron differentiation and maturation kit (#100-0038 and #100-0041; StemCell Technologies, New York, NY). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: Primary human CD4 and CD8 T cells were isolated from PBMCs by negative selection using STEMCELL EasySep isolation kits (Stemcell Technologies, 17952 and 17953), and mixed 1:1 ...
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bioRxiv - Biochemistry 2024Quote: ... PBMCs were isolated from the blood of healthy volunteer and enriched for CD3+ Tcells using EasySep™ human T-cell isolation kit (Stemcell Technologies, Vancouver,Canada) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25+ T cells were isolated from splenocytes of DR3 and DR3.IL17A-/- transgenic mice using EasySep™ Mouse CD4+CD25+ regulatory T cell isolation kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: CD4+CD25+ T cells isolated from the spleens of DR3 and DR3.IL17A-/- transgenic mice using the EasySep™ Mouse CD4+CD25+ regulatory T Cell Isolation Kit II (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... We used the commercial kit (STEMdiff™ Cerebral Organoid Kit, StemCell Technologies 08570) with previously described modifications to increase embryoid body surface area75 ...
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bioRxiv - Molecular Biology 2019Quote: ... Sl/Sl stromal cells (Stem Cell Technology), M2-10B4 stromal cells (Stem Cell Technology) ...
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bioRxiv - Molecular Biology 2019Quote: ... M2-10B4 stromal cells (Stem Cell Technology), and CD34+ HPCs were maintained as previously described (18) ...
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bioRxiv - Microbiology 2023Quote: ... M2-10B4 stromal cells (Stem Cell Technology), and CD34+ HPCs were maintained as previously described [19 ...
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bioRxiv - Microbiology 2023Quote: ... Sl/Sl stromal cells (Stem Cell Technology), M2-10B4 stromal cells (Stem Cell Technology) ...
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bioRxiv - Immunology 2022Quote: ... successfully transduced cells were enriched based on CD19t expression on day 11-15 using EasySep™ Human CD19 Positive Selection Kit II (Stemcell Technologies, Inc., Cat#17854), and the resulting CAR+ T cells were cryopreserved at day 15 or 17 ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: Samples were depleted of cells of an immune origin with the RosetteSep CD45 depletion kit (depletes with antibodies for CD45, CD66, and Glycophorin A, cat. no. 15122, Stem Cell Technologies, Cambridge, MA USA) according to the protocol with SepMate - 15 tubes (cat ...
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bioRxiv - Cell Biology 2024Quote: Primary human T cells were isolated from the peripheral blood of a healthy donor using a negative selection kit (EasySep, STEMCELL Technologies Canada Inc., Vancouver, BC) and used for testing the sensitivity of the lifetime flow system to T cell activation ...
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bioRxiv - Immunology 2024Quote: ILCs from Alt and Alt plus BPC treated mice were enriched from two single-cell suspended lungs by Mouse Pan-ILC Enrichment Kit (STEMCELL Technologies, Vancouver, British Columbia, Canada). ILC enriched samples were preserved in PIPseqTM T100 3L Single Cell Capture and Lysis Kit and processed by sequencing service (Fluent BioSciences ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were dissociated into single cells using AccutaseTM (Stem Cell technologies, Canada. Catalog# 07922) and transferred onto BIOLAMININ 521 LN (LN521 ...
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bioRxiv - Immunology 2019Quote: ... cells were separated with Lymphoprep (Stem Cell Technologies), following the manufacturer’s instructions and stained for flow cytometry ...
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bioRxiv - Bioengineering 2021Quote: ... cells were detached with ReLeSR (Stem Cell Technologies) at 37°C for about 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... cells were dissociated with Accutase (Stem Cell Technologies) and replated onto a monolayer of primary mouse glial cells on glass coverslips ...
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bioRxiv - Genetics 2022Quote: ... Cells were incubated with HetaSep (Stem Cell Technologies) at 5:1 ratio (cells 5 parts and HetaSep 1 part ...