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Citations for Macherey-Nagel GmbH :
401 - 450 of 454 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from the cultured cells using the Nucleospin RNA Plus kit (cat. 740984, Macherey-Nagel, CA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Three days later cells were isolated and either frozen or resuspended in RA1 buffer (NucleoSpin RNA XS, # 740902 Macherey-Nagel). The experiment was run in triplicates for treatment and vehicle ...
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bioRxiv - Immunology 2023Quote: Total RNA from BMDMs and RAW 264.7 cells was isolated using the NucleoSpin RNA II kit (Macherey-Nagel, Catalog #740955). The cDNA of pre-mRNA was synthesized by the use of random hexamer primers (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested in RIPA with 1X HALT or the RNA lysis buffer from the Nucleospin RNA kit (Macherey-Nagel).
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted in triplicate for mixed cell suspensions using either NucleoMag® DNA/RNA Water (Macherey-Nagel, Hoerdt, France), i.e. ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and lysed with RNA extraction buffer (Nucleo spin RNA extraction kit, Macherey-Nagel, Cat# 740955). Total RNA was extracted according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysis and extraction of genomic DNA from dry pellets were performed using the NucleoSpin Tissue XS kit (Macherey-Nagel). Genomic DNA quality and quantity were measured using the NanoDrop (ND-1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from wt and Uhrf1- or Usp7-knockdown BHK cells using the nucleospin triprep kit from Macherey-Nagel. 500 ng of total RNA was reverse transcribed with a high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Bioengineering 2021Quote: Cell pellets were washed and lysed using RA1 RNA extraction buffer and purified using the NucleoSpin RNA kit (MAcherey-Nagel; 740955.250) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
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bioRxiv - Microbiology 2021Quote: ... 30 min) to remove cell debris and the supernatant was collected and mixed with Protino Glutathione Agarose 4B (Macherey-Nagel # 745500.10) equilibrated with PBS buffer ...
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bioRxiv - Genomics 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin-RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were collected into the RA1 lysis buffer and extracted using the Nucleospin RNA Plus Extraction kit (740984, Macherey-Nagel).
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
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bioRxiv - Genomics 2020Quote: ... the genomic DNA from the edited cells was isolated after the transduction for eight days using a DNA isolation kit (NucleoSpinBlood - Macherey-Nagel). The target region for base editing was amplified using the respective primers (Sup Tables 2 and 3) ...
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bioRxiv - Immunology 2020Quote: Following stimulation cells were lysed and total RNA isolated using Nucleospin RNA purification columns according to the manufactures protocol (Macherey-Nagel). 0.5 to 1 µg of total RNA was reverse transcribed using BioRad iScript ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNAs were extracted from yeast cells disrupted by bead beating and purified using the Nucleospin RNA II kit (Macherey-Nagel). For RNA-seq analysis ...
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bioRxiv - Cell Biology 2021Quote: ... flanking Cas9 targeted site was amplified from genomic DNA extracted from wild type MCF10A cells (NucleoSpin tissue extraction kit, Macherey-Nagel), Puro-T2A were amplified from the custom-made plasmid MXS Puro bGHpA using primers containing T2A sequence ...
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bioRxiv - Cell Biology 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Immunology 2020Quote: ... was expressed in 293T cells from a plasmid kindly provided by Florian Krammer and purified using Ni-NTA Agarose (Macherey-Nagel), as previously published (Stadlbauer et al. ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from flow-sorted naive CD8+ T cells (n = 300 per condition) using a NucleoSpin RNA XS Kit (Macherey-Nagel), and cDNA was synthesized using Reverse Transcription Master Mix (Fluidigm) ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from mESCs and MEFs (3 biological replicates of WT and Atxn7l3−/− for each cell types) using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from U251 cells (SETD6 control vs. KO or Empty vs. TWIST1 WT) using the NucleoSpin RNA Kit (Macherey-Nagel). Samples were prepared in triplicates (SETD6 KO ...
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bioRxiv - Immunology 2022Quote: ... The surviving cells were expanded for 24 h and DNA was extracted using a NucleoSpin Tissue kit (Macherey-Nagel, Cat#740952). An additional eight biological replicates were similarly prepared but did not undergo killing by TC5A87 ...
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bioRxiv - Immunology 2022Quote: ... Transfection-grade plasmid DNA was isolated from XL10Gold cell overnight culture with the NucleoBond™ Xtra Midi Kit (Macherey-Nagel, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... The ligated products were transformed into XL1 blue bacterial cells by electroporation followed by isolating the plasmids from single bacterial colonies using NucleoBond Xtra Midi kit (Macherey-Nagel). The Cas9-sgRNA plasmids were validated by BbsI restriction analysis and confirmed by Sanger sequencing ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from control and IMCD PCM1 KO cells before SAG treatment and 24 h after SAG treatment using NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was added on top of the cell layer/bone powder and total RNA were purified with Nucleospin RNA set nucleozol column (Macherey-Nagel) according to the manufacturer recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from confluent cells from one well of a 6-well plate with the NucleoSpin RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from three replicates of PRMT7-KO2 and control PC3 cells was extracted using the NucleoSpin RNA kit (Macherey-Nagel). mRNA quality control checks and NGS for RNA-seq analysis were performed at the NIMgenetics facility (Madrid ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA was successfully isolated from blood cells of 34 14-day-old great tits with NucleoSpin RNA Plus Kit (Macherey-Nagel). Packed blood cells (10 µl per sample ...
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bioRxiv - Genomics 2023Quote: The genomic DNA was extracted from at least one million cells (typically 3 million, coverage 300x) using the NucleoSpin Tissue kit (Macherey-Nagel). To enrich for GBCs ...
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bioRxiv - Bioengineering 2023Quote: ... supernatant was removed and cell pellets were used for plasmid isolation by using NucleoBond□ Xtra Midi Plasmid DNA Purification Kit (Macherey-Nagel) according to the protocol supplied by the kit.
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from T-REx-293 and A549 cells using the NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel). 500 ng of total RNA was used for cDNA synthesis using Maxima™ Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: RNA from purified astrocytes or brain cell suspension after enzymatic digestion was extracted using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250), genomic DNA was eliminated and cDNA was synthetised (Thermo Fisher Scientific #K1672) ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated with Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Cells were lysed (RLT buffer with 1% β-mercaptoethanol) and RNA was isolated via column purification (NucleoSpin RNA kit, Macherey-Nagel 740955). RNA integrity and concentration were determined with BioAnalyzer (Agilent ...
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bioRxiv - Biophysics 2022Quote: ... Plasmid purifications for mammalian cell lines transfection were performed using high-yield column-based commercial NucleoBond Xtra Midi kit (Macherey-Nagel, Germany) following usage protocols.
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bioRxiv - Immunology 2023Quote: Total RNA was prepared from XG6 cells treated or not with IgG-ASO or CTR (48h, 3µM) using the NucleoSpin® RNA L kit (Macherey-Nagel, France) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The messenger RNA was purified from the total RNA of the spleen and bone marrow cells of immunized rabbits using the NucleoTrap mRNA kit (Macherey-Nagel, 740655) and according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from INS-1 832/13 cells 48h after transfection using the NucleoSpin extraction kit (Macherey-Nagel, Düren, Germany). The amount of isolated RNA was measured using the NanoDrop system ...
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bioRxiv - Cell Biology 2023Quote: ... the medium in the reservoirs was collected and the cells from the chambers were lysed with 350 µl Lysis Buffer RA1 (Macherey-Nagel, Germany) supplemented with 3.5 µl ß-mercaptoethanol (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... GRANTA-519 and B-cells purified from four MCL patients using the NucleoSpin® RNA II kit according to the manufacturer’s protocol (Macherey-Nagel, Oensingen, Switzerland). A minimum of 2µg per samples were used for analysis ...
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bioRxiv - Neuroscience 2021Quote: ... RNA from CD11B+ cells from brains of mice exposed to IL-1β or PBS at P5 were extracted (RNA XS plus, Macherey-Nagel, Dueren, Germany).
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bioRxiv - Molecular Biology 2022Quote: RNA from cell culture supernatant after CPZ/PCZ treatment (mentioned above) was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative RT-PCR was performed in Roche LightCycler 480 using commercial kits ...
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bioRxiv - Physiology 2021Quote: ... mRNA specimens were isolated from the cells grown on collagen-gel in 24-well dishes using a NucleoSpin RNAplus RNA isolation kit (Macherey-Nagel, Dueren, Germany) and converted to cDNA with SuperScript IV VILO Master Mix (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 x 109 cells were used for extraction of genomic DNA using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). PCR-free libraries (microbial short insert libraries ...
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bioRxiv - Bioengineering 2022Quote: ... 8×104 661W cells were seeded in a 24-well culture plate with DMEM and transfected with the plasmids containing guides using Lipofectamine™ Cells were harvested 72 hours following the transfection and Genomic DNA was then extracted using NucleoSpin® Tissue kit (Macherey-Nagel) following the manufacturer’s recommendation.
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... infected cells were washed five times to eliminate non-internalized VPs and DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel #740952-250). Internalized vector genomes were titrated by qPCR using a forward primer in the U exon ...