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Citations for Macherey-Nagel GmbH :
151 - 200 of 454 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected for 400-550 bp using a 2% agarose/TAE gel and column purification (Macherey-Nagel). Libraries were sequenced as above ...
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA from a male wild type blood sample (2 ml) was purified with the NucleoBand®HMW DNA Kit (Macherey-Nagel). To eliminate fragments below 40 kb the Short Read Elimination Kit XL (Circulomics ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were excised from 2% agarose gels and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) using NTC buffer for solubilization of the gel slices ...
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bioRxiv - Immunology 2021Quote: ... Solid phase extraction was carried out using Chromabond SiOH cartridges filled with 2 g of unmodified silica gel (Macherey-Nagel, Germany). Chemicals ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR products were confirmed on 2% agarose gels and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Purified PCR products were quantified using the Quant-iT dsDNA kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... or cells (NucleoSpin RNA plus kit, Macherey-Nagel) at different time post-electroporation (4 hpe – 7 dpe) ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed with Nucleozol (Macherey-Nagel, 740404) and RNA extractions were conducted according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated for 1 hour with 2 g Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany). The matrix was washed extensively with buffer L and bound protein was eluted in buffer L containing 250 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... and 6 µg total endotoxin free plasmid DNA (4 µg HaloTag-KIF5B1-560 plasmid DNA + 2 µg of α-tubulin-GFP) isolated using the NucleoBond Xtra Midi Plus EF kit (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were collected 5 days after transfection and the cell’s genomic DNA was prepared using NucleoSpin Tissue (MACHEREY-NAGEL & Co. KG).
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bioRxiv - Biochemistry 2020Quote: ... 4°C for 45 min) and the supernatant was incubated for 25 min at 4°C with 2 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µL of extracts were injected into a Nucleoshell RP 18 column (2.7 µm x 150 mm x 2 mm, Macherey-Nagel GmbH, Düren, Germany). A solvent system composed of A ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed with Nucleozol™ (Macherey-Nagel, 740404) and RNA extraction was conducted according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RNA was harvested from the cells (Macherey-Nagel; 740955) and subjected to cDNA synthesis with oligo(dT ...
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bioRxiv - Genetics 2023Quote: ... and then incubated for 2 h on ice on a rocking platform with 1 ml Protino Ni-NTA Agarose (MN, Macherey-Nagel GmbH, Düren, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH10 cells using a BAC prep kit (Macherey-Nagel). P19 Copg1-/- cells were transfected with 1µg BAC DNA using Effectene (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA purification from cultured cells and tissue (740955; Macherey-Nagel). Complementary cDNA was synthesised using Maxima first strand cDNA kit from five hundred nanograms of purified total RNA (K1641 ...
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bioRxiv - Biochemistry 2019Quote: Total RNA was extracted from HEK293T cells using NucleoZOL (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cells were collected in Nucleoprotect RNA solution (Macherey-Nagel, Dueren, Germany). Bacteria were gated as described above ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from cells using Nucleospin RNA (Macherey-Nagel; 740955) and cDNA was synthesized from 1 μg of total RNA using the M-MLV (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The cells were lysed with Nucleobond buffer set iii (MACHEREY-NAGEL) and the chromosomal DNA was purified with Sera-Mag magnetic beads (Cytiva) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified edited cells using a NucleoSpin Tissue Kit (Macherey-Nagel). The HDRT integration site was PCR-amplified from genomic DNA using Q5 High-Fidelity Polymerase (NEB) ...
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bioRxiv - Genomics 2021Quote: ... Menaquinones were then removed from the plate and analyzed using HPLC fitted with a reverse phase column (Macherey-Nagel, 2 mm x 125 mm, 3 µm, RP18) with methanol ...
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bioRxiv - Microbiology 2021Quote: ... half of the cells was lysed in RA1 lysis buffer (Macherey-Nagel) (“on ice”) ...
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bioRxiv - Microbiology 2020Quote: ... Total DNA was extracted from cell pellets using NucleoSpin Tissue (Macherey-Nagel) kit ...
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bioRxiv - Genetics 2019Quote: ... coli BL21-Gold cells and purified on Ni-NTA Agarose (Macherey-Nagel). Two guinea pigs were immunized at Bioscience with His-DBN-1(1-132 ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was collected from cells using the NucleoSpin RNA Kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: RNA from mESC and HEK293T cells was extracted with NucleoZOL (Macherey-Nagel); from MCF7 cells BIO TRI RNA reagent (Bio-lab ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then treated with RNase (Macherey-Nagel, Thermo Fisher Scientific), stained with propidium iodide (PI ...
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bioRxiv - Biophysics 2022Quote: ... coli MC1061 cell line using the Nucleobond Xtra Maxi Kit (Macherey-Nagel). 1.3 μg DNA was used per 106 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and processed using the NucleoSpin RNA kit (Macherey-Nagel) or the PureLink RNA Mini Kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was isolated from cell lines using NucleoSpin RNA Kit (Macherey-Nagel) and subjected to Real-Time PCR using the high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from cultured cells was isolated using NucleoSpin®RNA (Macherey-Nagel), Düren ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from cells using a NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: Genomic DNA was isolated from sorted cells using NucleoSpin Blood kits (Macherey-Nagel). PCR was used to amplify gRNA cassettes with Illumina sequencing adapters and indexes as described previously43 ...
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bioRxiv - Biochemistry 2020Quote: ... coli NM522 cells then extracted using a NucleoBond Xtra Midi kit (Macherey-Nagel). Quantification was performed using a SimpliNano spectrophotometer (Biochrom).
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bioRxiv - Cell Biology 2020Quote: Total DNA was extracted from cells using NucleoSpin Tissue (Macherey-Nagel, Duren, Germany). The extracted DNA (100 ng ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from cultured cells using Mini spin kit (Macherey-Nagel). RNA extracted from canine fibroblasts was obtained from Prof ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were either fixed with 4% paraformaldehyde or lysed with NucleoZOL (MACHEREY-NAGEL) 17 days post infection ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA of cells was isolated with Nucleo Spin miRNA Kit (Macherey-Nagel), total RNA of melanoma samples was isolated with RNeasy Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... cells were plated on sterile filters (0.2 µm pore size, MN615, Macherey-Nagel), placed on BG11 agar plates and incubated under standard conditions ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: RNA was extracted from S2 cells using Nucleospin RNA II kit (Macherey-Nagel) and cDNA was synthesized using SensiFAST cDNA Synthesis kit (Bioline ...