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Citations for Macherey-Nagel GmbH :
351 - 400 of 454 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted from cells corresponding to 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Immunology 2024Quote: ... gDNA from T cells and frozen liver biopsies was extracted with NucleoSpin® Blood kit (Macherey-Nagel) and TCRβ sequencing was performed as previously described45.
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bioRxiv - Cell Biology 2020Quote: ... Total RNA from cells of interest was isolated using the NucleoSpin RNA Kit from Macherey-Nagel (Düren, Germany) according to manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... RNA extraction (two replicates per cell line) was performed using the Nucleospin® RNA Plus Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was collected from the DT40 cells using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co., Germany) and subjected to complementary DNA synthesis and quantitative PCR using a ReverTra Ace qPCR RT Kit (Toyobo Life Science ...
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bioRxiv - Neuroscience 2021Quote: mRNAs of O4+ MACSorted cells were extracted using the Macherey-Nagel NucleoSpin RNA XS kit (740902.50, Macherey-Nagel) and quantified with Nanodrop spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from N/Tert or HFK cells using the NucleoSpin RNA extraction kit (Macherey-Nagel). RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from GYP treated GLUTag cells using the NucleoSpin® RNA kit (Macherey-Nagel, UK) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA was extracted using the NucleoSpin tissue mini kit for DNA from cells and tissue (Macherey-Nagel). DNA integrity was assessed by TapeStation (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA (gDNA) was isolated from sorted and unsorted cells using the Nucleospin Tissue Mini Kit (Macherey-Nagel) and sgRNA sequences were retrieved by a nested PCR approach that pre-amplified sgRNAs in a first round with primers specific to the lentiGuide-Puro construct (5’-AGAGGGCCTATTTCCCATGA-3’ ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from infected and non-infected HCT8 cells using NucleoSpin RNA Kit (Macherey-Nagel, Germany). An on-column DNase digestion with a RNase-free DNase was included in the process described by the fabricant to remove any genomic DNA contamination in RNA samples ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from 2.5×105 HEK293T cells was purified using a Nucleo Spin Tissue XS kit (MACHEREY-NAGEL). Using 25 mg of human postmortem tissue ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel, Hoerdt, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: To clone the NERCLIN cDNA we extracted total RNA from 143B cells using Mini spin kit (Macherey-Nagel). Then RNA was reverse transcribed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher scientific) ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was isolated using the NucleoSpin Tissue – Mini kit for DNA from cells and tissue (MACHEREY-NAGEL). Genomic DNA was sheared with a 30-G syringe ...
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bioRxiv - Microbiology 2022Quote: Total DNA was isolated from frozen cell pellets using the NucleoSpin Microbial DNA Kit (Macherey-Nagel, Düren, Germany). Methods for DNA quality control and quantification were reported previously [28] ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from the cells with a PGTX solution42 and purified using NucleoSpin RNA (MACHEREY-NAGEL). cDNA was synthesized from 1 μg of total RNA using ReverTra Ace qPCR RT Master Mix (TOYOBO) ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of homogenate was set aside as total cell (T) and mixed with NucleoZOL (Macherey-Nagel, #740404.200) before storage at -80°C ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... total RNA after McIdas RNAi was isolated from U2OS cells using the Nucleospin RNA II kit (Macherey-Nagel). 1 μg RNA was converted to cDNA using M-MLV transcriptase (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA were extracted from GBM control and EMP3 KO cells using the Nucleospin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the DNA of HEK293 cells was extracted and purified following the NucleoSpin Tissue protocol (Macherey-Nagel, Düren, Germany). The genotype at rs1047626 was verified to be heterozygote (AG ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from tissues or purified cells using the the NucleoSpin® RNA Plus Kit (Macherey-Nagel). Complementary DNAs were synthesized using the SuperScript™ III First-Strand Synthesis System Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were resuspended in PBS with 20 µg/ml PI and 0.2 mg/mL RNAse A (Macherey-Nagel) and incubated at RT for 30 min before analysis on a FACSCalibur (Beckton Dickinson) ...
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bioRxiv - Immunology 2023Quote: RNA extraction of in vitro cultured Th17 cells was performed with the NucleoSpin RNA XS Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: High molecular weight DNA was extracted from blood cells using a NucleoBond AXG column (Macherey-Nagel, Düren, Germany), which was followed by purification with phenol-chloroform ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was isolated from cells or tissues using NucleoSpin RNA Plus (Macherey-Nagel, Duren, Germany, Cat. No. 740984.250) and subjected to cDNA synthesis using ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... and the selection of inactivated cells was performed by DNA extraction using the NucleoSpin Blood kit (Macherey-Nagel) followed by PCR amplification using primers flanking the Cas9 cleavage site ...
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bioRxiv - Cancer Biology 2024Quote: ... SNU-499 and Hep3B cells was extracted using the NucleoSpin RNA kit (Macherey-Nagel, AH Diagnostics, Solna, Sweden) according to the protocol of the manufacturers ...
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bioRxiv - Immunology 2021Quote: RNA of polyclonally activated CD8+ T cells from C57BL6/N mice was purified using NucleoSpin RNA kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: Total RNA was extracted from 2.3×106 Sf9 cells in each sample using NucleoSpin® RNA kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was extracted from GYP pre-treated BRIN-BD11 cells using NucleoSpin® RNA kit (Macherey-Nagel, UK) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: Cells were harvested and DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Libraries for whole genome sequencing were prepared with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Immunology 2022Quote: ... RNA was isolated from peripheral blood mononuclear cells (PBMC; NucleoSpin RNA Mini Plus, Macherey-Nagel GmbH & Co.KG, Düren, Germany). For cDNA first strand synthesis RevertAid kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from TGF-β1-treated cells (as described above) using NucleoSpin® RNA kit (Macherey-Nagel) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: RNA from sorted blasts or ex vivo-cultured cells was isolated using NucleoSpin RNA XS kit (#740902 Macherey-Nagel) or RNeasy Mini Kit (#74104 Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Cells were treated as indicated for 72 hours prior to harvesting of RNA using the Nucleospin RNA kit (Macherey-Nagel). First-strand cDNA synthesis was performed using iScript Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2019Quote: ... lysates prepared from infected and stimulated cells were collected and RNA extracted using a NucleoSpin RNA kit (Macherey-Nagel, 740955). The yield and purity of the extracted RNA was assessed using a spectrophotometer (DS-11 FX ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin® RNA II kit according to the manufacturer's protocol (Macherey-Nagel). Sixty nanograms of total RNA were reverse transcribed by using the RevertAid H Minus First Strands cDNA Synthesis Kit with oligo(dT ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from sorted E14.5 fetal liver hematopoietic progenitor cells (KL; CD45+/Lineage−/cKit+/Sca1−) using the NucleoSpin RNA Plus XS kit (Macherey-Nagel). The RNA-sequencing libraries were prepared using KAPA RNA Hyper Prep Kit with RiboErase ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 16 h after transfection and total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2021Quote: ... The final transgenic BAC DNA is then transferred back to DH10b cells for better extraction yield (Nucleobond BAC100, Macherey-Nagel). DNA is resuspended in 10mM Tris-HCL pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The final transgenic BAC DNA is then transferred back to DH10b cells for better extraction yield (Nucleobond BAC100, Macherey-Nagel). DNA is resuspended in injection buffer (10mM Tris-HCL pH 7.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... After 16 hours cells were lysed and total RNA was isolated using NucleoSpin® RNA Plus Mini Kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from around 5×105 to 1×106 cells using the NucleoSpin RNA Plus (MACHEREY-NAGEL, 740984.50) or Quick-RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the cells were collected from the plate and plasmid pools were extracted using the NucleoBond Xtra Midi Kit (Macherey-Nagel) according to the manufacturer’s recommendation.
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bioRxiv - Molecular Biology 2021Quote: ... His6-BsrA was purified from the cell lysate by chromatography on Ni-agarose columns (Protino Ni-TED 1000, Macherey-Nagel) with 300 mM imidazole in phosphate buffer used for elution ...
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bioRxiv - Neuroscience 2021Quote: Cell lysis and RNA extraction were performed on macrophage samples 24h post-activation using Nucleospin RNA extraction kit (Macherey-Nagel). Quality of RNA was confirmed on Agilent TapeStation (RINe>8) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from ΔbolA and control cell line was isolated independently six times using NucleoSpinTM RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: We extracted RNA from live cells for bulk RNA-sequencing using the NucleoSpin totalRNA FFPE micro kit (Macherey-Nagel #740969.50) ...