Labshake search
Citations for Macherey-Nagel GmbH :
2101 - 2150 of 2219 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Zoology 2020Quote: ... urticae (one butterfly individual per nest) collected across 8 sites spread across the latitudinal gradient using the NucleoSpin® 96 Tissue kit (Macherey-Nagel) (Figure 1) ...
-
bioRxiv - Plant Biology 2019Quote: ... Extraction of total genomic DNA was conducted with the NucleoSpin Plant Kit in accordance to the manufacturer’s protocol (Macherey-Nagel, Düren, Germany). The concentration of the DNA samples was checked with a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2019Quote: ... Total genomic DNA was extracted from a fresh stem slice using NucleoSpin® Plant II DNA extraction kit (Macherey-Nagel, Düren, Germany) and quantified using a Qubit 2.0 device (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... to reach at least 200x colonies per guide-RNA and the library plasmid pool was purified using NucleoBond Xtra-Maxi Kit (Macherey-Nagel, #740414.50). Then ...
-
bioRxiv - Microbiology 2020Quote: Lung left inferior lobes from hamsters were cut into pieces and lysed using the RA1 lysis buffer provided with the NucleoSpin® RNA Plus kit (Macherey-Nagel). RNA extraction was then performed according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... DNA extraction was performed on (i) the frozen suspension and (ii) the collected CFU suspension with the NucleoSpin® 96 Food kit (Macherey-Nagel) following the supplier’s recommendations.
-
bioRxiv - Genomics 2021Quote: Sequencing libraries were prepared from the cells collected at the end of the screens as follows: Genomic DNA was extracted using the NucleoSpin® Blood XL kit (Macherey-Nagel) with a condition of 50e6-100e6 cells per column ...
-
bioRxiv - Genomics 2019Quote: ... CHP-100 and HS-SYII) and tissue (ES1 and RH) was extracted by using the column-based NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Total RNA was isolated from 2 × 105 cells untreated or butyrate-conditioned BMDMs using the NucleoSpin RNA extraction kit (Macherey-Nagel, 740902.50) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... and 6 µg total endotoxin free plasmid DNA (4 µg HaloTag-KIF5B1-560 plasmid DNA + 2 µg of α-tubulin-GFP) isolated using the NucleoBond Xtra Midi Plus EF kit (MACHEREY-NAGEL GmbH & Co ...
-
bioRxiv - Cell Biology 2022Quote: ... MuSCs from the gaskin muscles were collected by FACS and then subjected to extract RNA with NucleoSpin® RNA XS kit from Macherey-Nagel. 1 ng high-quality RNA per sample were reverse transcribed and amplified into cDNA following Smart-seq2 library preparation protocol (Picelli et al. ...
-
bioRxiv - Immunology 2022Quote: ... 5000-10 000 ThyMCs were sorted into lysis buffer and the RNA was extracted using the NucleoSpin® RNA XS RNA isolation kit (Macherey-Nagel). Total RNA was reverse transcribed into cDNA using the SuperScript IV First-Strand Synthesis System (Thermo Fisher) ...
-
bioRxiv - Microbiology 2022Quote: Total RNA was isolated from mock- and KSHV-infected blood or lymphatic ECFCs using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). RNA was further concentrated and purified using the RNA Clean and Concentrator kit (Zymo Research ...
-
bioRxiv - Neuroscience 2022Quote: Cells were lysed (RLT buffer with 1% β-mercaptoethanol) and RNA was isolated via column purification (NucleoSpin RNA kit, Macherey-Nagel 740955). RNA integrity and concentration were determined with BioAnalyzer (Agilent ...
-
bioRxiv - Plant Biology 2022Quote: ... O23 and d44a genomic DNA was extracted from liquid-cultured aerial hyphae using the NucleoBond high-molecular-weight DNA kit (MACHEREY-NAGEL, Germany). The genomic DNA was processed through the short-read eliminator kit XL (Circulomics) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The resulting powder was used for DNA extraction using the NucleoSpin® Tissue kit following manufacturer instructions (MACHEREY-NAGEL GmbH & Co. KG). DNA quantity and purity were checked with a Nanodrop One spectrophotometer (Thermo Scientific ...
-
bioRxiv - Biophysics 2022Quote: ... Plasmid purifications for mammalian cell lines transfection were performed using high-yield column-based commercial NucleoBond Xtra Midi kit (Macherey-Nagel, Germany) following usage protocols.
-
bioRxiv - Immunology 2023Quote: Total RNA was prepared from XG6 cells treated or not with IgG-ASO or CTR (48h, 3µM) using the NucleoSpin® RNA L kit (Macherey-Nagel, France) according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... The messenger RNA was purified from the total RNA of the spleen and bone marrow cells of immunized rabbits using the NucleoTrap mRNA kit (Macherey-Nagel, 740655) and according to the manufacturer’s protocol ...
-
bioRxiv - Physiology 2023Quote: ... six samples each containing 5 whole larvae or 5 larval dissected tissues (central neuronal systems or body carcasses) were disrupted in 350 μl lysis buffer from the NucleoSpin RNA kit (Macherey-Nagel, #740955) containing 1% beta-mercaptoethanol ...
-
bioRxiv - Immunology 2023Quote: Total RNA was extracted from microglia after CD11b microbead isolation from P7 neonates born to and nursed by WT and Slc2a5−/− dams using the NucleoSpin RNA kit (Macherey-Nagel, 740955.250). The QuantiTect Reverse Transcription Kit (Qiagen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Bacteria were grown in Luria-Bertani Broth at 37°C and plasmid DNA was isolated using the Nucleobond Xtra midi kit (MACHEREY-NAGEL, 740410), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA gene of Ca ...
-
bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
-
bioRxiv - Genetics 2023Quote: Genomic DNA of the 54 genotypes was extracted from the leaves using a mixed alkyltrimethylammonium bromide buffer and the kit NucleoMag Plant (Macherey-Nagel, Germany). DNA samples were quantified using ThermoFisher Scientific fluorometry Qubit ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from INS-1 832/13 cells 48h after transfection using the NucleoSpin extraction kit (Macherey-Nagel, Düren, Germany). The amount of isolated RNA was measured using the NanoDrop system ...
-
bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from 8–12 mg of dried tissue using the NucleoSpin 96 Plant II kit (Macherey-Nagel GmbH & Co. KG) following manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... The resulting constructs were used for protoplast transformation through midiprep using the NucleoBond Xtra plasmid kit (Macherey-Nagel GmbH & Co.KG, Duren, German) as per the manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2023Quote: ... 700ng of the plasmid library was isolated from the liquid culture using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany).
-
bioRxiv - Genomics 2023Quote: Perca fluviatilis gDNA samples from 35 males and 35 females were extracted with the NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany), following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: The total RNA of the brain samples was extracted according to the manufacturer’s protocol by applying the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The quality and quantity of RNA were assessed at 260 nm using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2023Quote: Bulk DNA of the meristematic region and the basal portions of the leaves was extracted using NucleoSpin Plant II kit (Macherey-Nagel, Germany). DNA concentration was determined using a Qubit Fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
-
bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from six-day-old seedlings (approximately 20 seedlings per genotype) using the NucleoSpin RNA plant kit (Macherey-Nagel CO.). Subsequently ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The isolation of gDNA was conducted either by using a column-based method following manufacturer’s instructions (NucleoSpin Microbial DNA kit, Macherey-Nagel, Düren, Germany) or an automated system (King Fisher Duo Prime System ...
-
bioRxiv - Cancer Biology 2023Quote: ... the cultured alone C4-2B cells or the mixed co-cultured C4-2B/MC3T3-E1 were harvested for DNA extraction using the NucleoSpin Tissue kit (Macherey-Nagel Inc.). Primers against the nuclear and mitochondrial genomes were used for qRT-PCR (sequences provided in Supplementary Table S2 ...
-
bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
-
bioRxiv - Zoology 2024Quote: ... total RNA was extracted from the second pooled sample of 60 bees using a NucleoSpin®RNA kit (Macherey-Nagel Gmbh & Co.), cDNA was synthesized from 800 ng total RNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The three constructs were then cultured for 24 h at 37°C and purified with NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) and used for gene reporter assay.
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in water ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL 1X TE buffer ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... and we collected bands containing arrays ≥2-plex at 550-650 bps and 650-800 bps to be purified separately using a NucleoSpin Gel Purification Kit (Macherey-Nagel 740609.250). We also collected the cut vector backbone ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL water ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... to pellet the cell debris and the RNA was extracted from the supernatant using the two column-system of the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Germany). DNA removal was ensured by the on-column DNA digestion according to the manufacturer’s description ...
-
bioRxiv - Genetics 2022Quote: ... DNA was then extracted from tissue using the NucleoSpin Tissue Mini kit columns and corresponding protocol for extraction from animal tissue (Macherey-Nagel, Duren, Germany). For each sperm pool ...
-
bioRxiv - Microbiology 2019Quote: Environmental DNA was extracted from 0.3 g of crushed rocks using NucleoSpin® Plant II Kit (Macherey-Nagel, Gmbh & Co. KG, Duren, Germany) according to the manufacturer’s instructions and quantified by Quant-iT dsDNA HS assay kit (Invitrogen molecular probes-Eugene ...
-
bioRxiv - Cancer Biology 2020Quote: ... GRANTA-519 and B-cells purified from four MCL patients using the NucleoSpin® RNA II kit according to the manufacturer’s protocol (Macherey-Nagel, Oensingen, Switzerland). A minimum of 2µg per samples were used for analysis ...
-
bioRxiv - Biochemistry 2019Quote: Genomic DNA was isolated using the NucleoSpin® RNA II and NucleoSpin® RNA/DNA Buffer Set kit (MACHEREY-NAGEL, Düren, Mannheim, Germany) kit according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... were extracted from fresh haploid mycelium after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel, Düren, Germany). Sequencing was performed using the Illumina HiSeq 2500 paired-end technology (Illumina Inc. ...