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Citations for Macherey-Nagel GmbH :
1851 - 1900 of 2219 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 37°C and then cleaned up with the NucleoSpin RNA clean-up kit (Macherey-Nagel). RNA quality was verified on a 2100 Bioanalyzer Instrument (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: RNA from nasal washings and organ samples was extracted using the NucleoMag® VET Kit (Macherey-Nagel, Düren, Germany) in combination with a Biosprint 96 platform (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood using NucleoSpin Blood Quick Pure kit (Macherey-Nagel, Duren, Germany) and was stored in the gene bank run by Azabu University (Kanagawa ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: RNA isolation for multiple tissues was achieved by using the NucleoZOL one phase RNA purification kit (Macherey-Nagel #740404.200) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... All constructs were verified by sequencing and purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Total RNA extraction and complementary DNA (cDNA) synthesis were performed using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany) and High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from TGF-β1-treated cells (as described above) using NucleoSpin® RNA kit (Macherey-Nagel) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: All expression constructs generated in this work were then midi-prepped using NucleoBond Xtra Midi kit (Macherey-Nagel 740410.50).
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bioRxiv - Genetics 2023Quote: ... except for genomic DNA for whole-genome sequencing that was extracted using NucleoSpin DNA purification kit (MACHEREY-NAGEL®).
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bioRxiv - Cancer Biology 2023Quote: RNA from sorted blasts or ex vivo-cultured cells was isolated using NucleoSpin RNA XS kit (#740902 Macherey-Nagel) or RNeasy Mini Kit (#74104 Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then lysed in Buffer RA1 and RNA was extracted with NucleoSpin RNA kit (Macherey-Nagel ref 740902.250). Total RNAs were then retrotranscribed to cDNA using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent ref 200436 ...
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bioRxiv - Bioengineering 2023Quote: ... Library plasmids were harvested from transformed bacteria and purified using the NucleoBond Xtra MIDI plasmid preparation kit (Macherey-Nagel). The AAV library genomes were packaged into 293T cells by transfecting 1 × 107 in ten 15-cm with the library plasmids (containing the cap gene with peptide insertion ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from 50µm cryostat sections of left ventricular tissue samples with NucleoMag Tissue Kit (Macherey-Nagel, Germany) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: DNA was extracted from 20 mg of dried leaf tissue using the NucleoSpin Plant II kit (Macherey-Nagel, Denmark). DNA was extracted according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Plant Biology 2023Quote: RNA was isolated from 6-days-old seedlings using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted genomic DNA from faeces using the NucleoSpin®Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Cancer Biology 2023Quote: Total DNA was extracted from various mouse organs using the NucleoSpin Tissue kit (Macherey-Nagel Inc., Allentown, PA, USA). The DNAs were used for species-specific PCR with primers against human α-satellite and mouse plakoglobin genomic regions [35] ...
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bioRxiv - Evolutionary Biology 2023Quote: ... They have been further purified using the Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al. 2011). The extracts were subsequently diluted in water and quantified with a Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... DNA isolations were conducted using the NucleoSpin Plant II Mini Kit for DNA from plants (Macherey-Nagel, Düren, Germany). Preliminary quality assessments were conducted using agarose gel visualization and NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was extracted from ∼300 mg of soil using the NucleoSpin Soil 96 kit (Macherey-Nagel, Düren, Germany). Lysis was performed using the Buffer SL with Enhancer SX and on the FastPrep 24 homogenizer (MP Biomedicals ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 100 mg of ground tissue using the NucleoSpin RNA Plant Kit (Macherey-Nagel) and treated with DNase I on the column ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from DIV4 or DIV6 primary neuronal cultures using the NucleoSpin RNA purification kit (Macherey-Nagel) and from human fibroblasts using Trizol reagent (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: BAC DNA was extracted from BAC clones (BacPac Resources, CHORI, Oakland) using the Nucleobond Xtra BAC kit (Macherey-Nagel) and subsequently labeled (Nick translation protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Molecular Biology 2024Quote: ... and Agilent microarray analyses were performed from isolated total RNA using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250). Per reaction 200 ng RNA was amplified and Cy3-labeled using the LIRAK kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA containing the ACE-tRNA TTT sequences was midiprepped with the NucleoBond PC 20 miniprep kit (Macherey-Nagel). 16HBE14o-cells were diluted to 6 x 105 cells per mL with 1 mL added per well of a coated 6-well plate as previously described22 ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from 100 mg of ripe fruits using the Nucleo-spin RNA Plant mini kit (Macherey-Nagel). Library preparation and Nanopore sequencing were performed at the Ecole normale superieure genomic core facility (Paris ...
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bioRxiv - Cancer Biology 2021Quote: Cells were treated as indicated for 72 hours prior to harvesting of RNA using the Nucleospin RNA kit (Macherey-Nagel). First-strand cDNA synthesis was performed using iScript Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: RNA was purified from cerebellum samples from Cockayne patients and age- and gender-matched healthy individuals by using the Nucleospin RNA isolation kit (catalog no. 740955.250; Macherey-Nagel) with initial quantitation conducted using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... lysates prepared from infected and stimulated cells were collected and RNA extracted using a NucleoSpin RNA kit (Macherey-Nagel, 740955). The yield and purity of the extracted RNA was assessed using a spectrophotometer (DS-11 FX ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from total thymocytes and from sorted T-cell subsets from MCMV-infected and uninfected mice according to the manufacturer’s instructions using the NucleoSpin Blood QuickPure kit (MACHEREY-NAGEL), and stored at −20°C until further analysis.
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bioRxiv - Immunology 2021Quote: ... Virus was purified by centrifugation at 12,000 xg for 10 mins at 4 °C and genomic DNA extracted (NucleoSpin Tissue kit, Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin® RNA II kit according to the manufacturer's protocol (Macherey-Nagel). Sixty nanograms of total RNA were reverse transcribed by using the RevertAid H Minus First Strands cDNA Synthesis Kit with oligo(dT ...
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bioRxiv - Genetics 2019Quote: Total RNA was extracted from ovaries of 3-day-old females using the NucleoSpin® RNA isolation kit (Macherey-Nagel), following the instructions of the manufacturer ...
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bioRxiv - Microbiology 2019Quote: Total cellular RNA from well-differentiated hAECs was extracted with the Nucleospin™ RNA extraction kit (Macherey-Nagel, Oensingen, Switzerland) according to the manufacturer’s guidelines ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted using the NucleoSpin Plant II Mini kit according to the manufacturer’s instructions (Macherey-Nagel, Hoerdt, France) with the following exception for the lysis step ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted DNA from recently collected field and greenhouse samples using NucleoSpin Plant Mini Kit II (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: Total RNA from pools of 5 oocytes were extracted using the Nucleospin RNA XS kit according to the manufacturer’s protocol (Macherey-Nagel) and including a DNase1 treatment ...
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bioRxiv - Biochemistry 2020Quote: ... Genomic DNA was isolated from the pellet of trophozoite/schizont stage parasites using the Nucleospin® tissue kit (Macherey-Nagel) as instructed by the manufacturer.
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from sorted E14.5 fetal liver hematopoietic progenitor cells (KL; CD45+/Lineage−/cKit+/Sca1−) using the NucleoSpin RNA Plus XS kit (Macherey-Nagel). The RNA-sequencing libraries were prepared using KAPA RNA Hyper Prep Kit with RiboErase ...
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bioRxiv - Genetics 2021Quote: ... Each tissue sample was sonicated for a few seconds with the buffer LBP1 from NucleoSpin RNA Plus kits (MACHEREY-NAGEL) and divided into two tubes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
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bioRxiv - Microbiology 2021Quote: ... as well as cKD TgISU1 (incubated with or without ATc at 1.5 μg/mL for 3 days) were extracted using Nucleospin RNA II Kit (Macherey-Nagel). The cDNAs were synthesized with 450 ng of total RNA per RT-PCR reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 16 h after transfection and total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2021Quote: ... cells were stimulated with 15% FBS for 45 min and total RNA was extracted using Nucleospin RNA II kit according to the manufacturer’s protocol (Macherey-Nagel). Five hundred micrograms of total RNA wasused for complementary DNA synthesis using Thermo Scientific RT–PCR kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... After 16 hours cells were lysed and total RNA was isolated using NucleoSpin® RNA Plus Mini Kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted by using the Macherey-Nagel Nucleospin® RNA Clean-up XS kit (Macherey-Nagel, Bethlehem, PA). cDNA was synthesized using iScript cDNA synthesis kit (Bio-Rad ...