Labshake search
Citations for Macherey-Nagel GmbH :
1801 - 1850 of 2219 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... and subjected to genomic DNA extraction using the Nucleo Mag Plant kit (Macherey-Nagel GmbH &Co. KG, Germany) in the automated nucleic acid extractor Maelstrom 4800 (TANBead Taiwan Advanced Nanotech Inc. ...
-
bioRxiv - Microbiology 2023Quote: ... and the selection of inactivated cells was performed by DNA extraction using the NucleoSpin Blood kit (Macherey-Nagel) followed by PCR amplification using primers flanking the Cas9 cleavage site ...
-
bioRxiv - Molecular Biology 2024Quote: ... A 200 ul aliquot was used for RNA extraction using a NucleoMag Virus kit (Macherey-Nagel, Düren, Germany) and a Magnetic Particle Processor (MPP ...
-
bioRxiv - Microbiology 2024Quote: ... 0.5 g of soil were used for DNA extraction using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... RNA samples were purified with the rDNase set and the RNA Clean-up kit (Macherey-Nagel, Düren, Germany). RNA quality was checked with the Prokaryote Total RNA Nano Series II chip using the Agilent Bioanalyzer 2100 (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... extracted by using the NucleoSpin® Plasmid or NucleoBond® Xtra Midi Kit (Macherey-Nagel; #740588.250 and #740410.50) and controlled by Sanger Sequencing (Eurofins Genomics or Seqlab Microsynth).
-
bioRxiv - Neuroscience 2024Quote: ... Maxi-preps of the plasmid construct were isolated using a Nucleobond Xtra Maxi Plus EF kit (Macherey-Nagel). The full coding frame of all pRK5-rPanx1 and pRK5-mPanx1 constructs were sequenced at the University of Calgary Sequencing Core using the primers CMV-F (5′-CGCAAATGGGCGGTAGGCGTG-3′ ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pUC57 mini plasmid used as carrier cDNA for transfections was gigaprepped as per kit instructions (Macherey-Nagel). The normalized ACE-tRNA library cDNA at 50 ng/μL was mixed at equal volumes with pUC57 mini carrier plasmid at 150 ng/μL to give final concentrations of 25 ng/μL and 75 ng/μL of the library and carrier plasmids respectively.
-
bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted using the Nucleospin Blood L Midi genomic DNA extraction kit (Macherey-Nagel, 740954.20). Genomic DNA was PCR amplified by the Genetic Perturbation Platform at the Broad Institute using the following primers:
-
bioRxiv - Biochemistry 2024Quote: Fosmid DNA of the hit clones was extracted with the NucleoBond Xtra Midi kit from Macherey-Nagel (France) following supplier recommendations ...
-
bioRxiv - Cancer Biology 2024Quote: ... SNU-499 and Hep3B cells was extracted using the NucleoSpin RNA kit (Macherey-Nagel, AH Diagnostics, Solna, Sweden) according to the protocol of the manufacturers ...
-
bioRxiv - Cancer Biology 2024Quote: RNA was isolated using the NucleoSpin® RNA Plus kit following the manufacturer’s protocol (Macherey-Nagel, Düren, Germany). The integrity and concentration of RNA samples was assessed using the TapeStation 2200 (Agilent ...
-
bioRxiv - Systems Biology 2020Quote: The viral RNA from supernatant of infected cells was isolated using the NucleoSpin RNA virus isolation kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... were grown overnight at 37 °C and plasmid was subsequently purified using the NucleoBond Xtra Midi kit (Macherey-Nagel). Plasmid DNA was solubilized in 150 µl of TE buffer and quantified using NanoDrop ND-1000 spectrophotometer (Celbio) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fragments of 300–2000 bp were excised from the gel and isolated with a gel-extraction kit (Macherey-Nagel, NucleoSpin Gel and PCR Clean-up ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA for short-read sequencing was extracted using NucleoSpin® Tissue kit (Macherey-Nagel, GmbH & Co. KG, Germany), library was prepared by Nextera® XT Library Preparation kit (Illumina ...
-
bioRxiv - Molecular Biology 2021Quote: ... The in vitro transcripts (IVTs) were cleaned up using the NucleoSpin RNA Clean-Up Kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions and subsequently quantified by using a Qubit RNA BR Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: RNA from sorted BMDCs was extracted using the Macherey-Nagel™ NucleoSpin™ RNA Plus kit (Macherey-Nagel™) and quantified using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Genetics 2019Quote: ... according to the manufacturer’s protocol followed by concentration of RNA using NucleoSpin RNA clean-up XS kit (Macherey-Nagel). The quantity and quality of RNA was determined using both NanoDrop Spectrophotometer ND-1000 (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... Bacteria were collected and the plasmid DNA was purified using NucleoBond® Xtra Midi kit (MACHEREY-NAGEL, cat. 740410.50). The transformation efficiency was estimated by plating 1/10000 of the transformation reaction onto 15 cm LB-agar plate with 100 µg/ml ampicillin and counting number of clones after incubation overnight at 37°C.
-
bioRxiv - Cancer Biology 2019Quote: ... Total RNA were purified after 72 hours incubation with a Nucloespin RNA purification kit (Macherey-Nagel cat. no. 740955) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: ... Total RNA was extracted from 400 µL whole blood using the NucleoSpin RNA Blood Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s directions ...
-
bioRxiv - Genetics 2021Quote: Total RNA was extracted from ovaries of 3-day-old females using the NucleoSpin RNA isolation kit (Macherey-Nagel), following the instructions of the manufacturer ...
-
bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA extraction was performed on the whole guts of five workers using the NucleoSpin Soil kit (Macherey-Nagel) according to manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... The vRNA in the cell culture supernatant was purified using a NucleoSpin® RNA virus kit (Macherey-Nagel, 740956) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from six human fecal samples using the NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Germany), as described previously [36] ...
-
Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... infiltrated leaves were harvested after 6 h for total RNA isolation with NucleoSpin® RNA Plus Kit (Macherey-Nagel). First strand cDNA was synthesized with RevertAid™ M-MuLV reverse transcriptase (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: The total RNA was isolated from macrophages (infected and uninfected) using Nucleo spin RNA extraction kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... The supercoiled pUC19 (pUC19sc) was extracted from 100 ml overnight cultures using the NucleoBond Xtra Midi kit (Macherey-Nagel) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from 10 ml of culture using the NucleoSpin RNA kit (Macherey-Nagel Inc., PA, USA) as described by the manufacturer ...
-
bioRxiv - Cancer Biology 2020Quote: Genomic DNA (gDNA) was isolated from mouse ear punches and hepatocytes with the NucleoSpin Tissue Kit (Macherey-Nagel, Germany). RNA was isolated from mouse liver with peqGOLD RNAPure reagent (VWR ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from iPSCs and from induced neurons after 21 DIV using RNA Plus Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... In vitro transcribed mRNAs were purified using Macherey-Nagel Nucleospin® RNA Clean-up kit (Macherey-Nagel, Bethlehem, PA) prior to injections.
-
bioRxiv - Immunology 2022Quote: ... coli strain in presence of 100 μg/ml ampicillin and prepared by Midi DNA-prep kit (Macherey-Nagel, 740410) for the transient transfection ...
-
bioRxiv - Microbiology 2020Quote: Total cellular RNA from mock and virus-infected hAEC cultures was extracted with the NucleoMag RNA kit (Macherey-Nagel) according manufacturers guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
-
bioRxiv - Immunology 2021Quote: RNA of polyclonally activated CD8+ T cells from C57BL6/N mice was purified using NucleoSpin RNA kit (Macherey-Nagel) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... RNA was extracted from wing discs dissected on ice using the NucleoSpin® RNA XS Kit (Macherey-Nagel, 740902.50). Control wing discs expressed UAS::myr-GFP instead of the RNAi ...
-
bioRxiv - Developmental Biology 2019Quote: ... genomic DNA was isolated from a confluent 3.5- or 10-cm dish with the Nucleospin Tissue Kit (Macherey-Nagel). The rearranged region between the promoter and GOIs (500-600 bp ...
-
bioRxiv - Synthetic Biology 2020Quote: Total RNA was extracted from 2.3×106 Sf9 cells in each sample using NucleoSpin® RNA kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was extracted from GYP pre-treated BRIN-BD11 cells using NucleoSpin® RNA kit (Macherey-Nagel, UK) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: DNA from colon content was extracted using the NucleoSpin® Soil kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) according to the manufacturer’s recommendations ...
-
bioRxiv - Genomics 2020Quote: Total protein was extracted from differentiated neurons at DIV 35 with the NucleoSpin RNA/Protein Kit (Macherey-Nagel 740933) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: Cells were harvested and DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Libraries for whole genome sequencing were prepared with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs ...
-
bioRxiv - Developmental Biology 2022Quote: The RNA form resected fin pieces was extracted using NucleoSpin RNA Plus XS kit for RNA purification (Macherey-Nagel) according manufacturers protocol ...
-
bioRxiv - Immunology 2022Quote: ... RNA from dry pellets of Vγ9Vδ2 T lymphocytes was extracted and purified using the NucleoSpin RNA plus isolation kit from Macherey-Nagel. DGE-sequencing was performed by the BIRD core facility and data were analyzed using R programming languages and tools.
-
bioRxiv - Genetics 2022Quote: Testis DNA extraction was carried out for each individual testis piece using the NucleoMag Tissue Kit (Macherey-Nagel, #744300.1) according to the manufacturer’s conditions except for a few modifications ...
-
bioRxiv - Molecular Biology 2022Quote: ... We extracted RNA from the sampled brain and testis using the NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG), and the pituitary using the NucleoSpin RNA Clean-up XS kit (Macherey-Nagel GmbH & Co ...
-
bioRxiv - Microbiology 2022Quote: ... One hundred mg of soil were used as starting material using the NucleoSpin Soil DNA kit (Macherey-Nagel, Germany). The whole procedure was carried out under laminar flow ...
-
bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
-
bioRxiv - Cancer Biology 2022Quote: ... plasmid DNAs were recovered from ampicillin resistant colonies using a kit (NucleoBond Xtra Midi, Macherey-Nagel GmbH, Düren, Germany), and the nucleotide sequences of each construct confirmed as correct by Sanger direct cycle sequencing using a U6-specific sequencing primer.