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Citations for Macherey-Nagel GmbH :
701 - 724 of 724 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: DNA extraction was performed from a single female 4th instar larva (ToLID ilPecGoss1) using the NucleoMag Tissue kit (Macherey-Nagel, Allentown, PA, USA). The high molecular weight DNA was prepared for PacBio sequencing using the SMRTbell Express Template Prep Kit 2.0 for Continuous Long Read (CLR ...
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bioRxiv - Microbiology 2022Quote: ... The total genomic DNA of the insect individual and its symbionts was extracted using the NucleoSpin Tissue XS Kit (MACHEREY-NAGEL, Duren, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
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bioRxiv - Bioengineering 2022Quote: ... 8×104 661W cells were seeded in a 24-well culture plate with DMEM and transfected with the plasmids containing guides using Lipofectamine™ Cells were harvested 72 hours following the transfection and Genomic DNA was then extracted using NucleoSpin® Tissue kit (Macherey-Nagel) following the manufacturer’s recommendation.
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bioRxiv - Molecular Biology 2023Quote: ... 500 µg of ground mycelium was used for extraction of high molecular weight DNA with the NucleoBond HMW-Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... infected cells were washed five times to eliminate non-internalized VPs and DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel #740952-250). Internalized vector genomes were titrated by qPCR using a forward primer in the U exon ...
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bioRxiv - Genetics 2023Quote: 200K cells were collected on day 14 after crRNA transduction and genomic DNA was isolated using NucleoSpin Blood (Macherey-Nagel, Catalog no. 740951.50). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... If no genomic DNA could be obtained strains were cultured according to the manufacturer’s protocol and DNA was extracted using the NucleosSpin Plasmid (NoLid) Mini kit (Macherey-Nagel cat. No. 740499) with vortexing after addition of BufferA2 and BufferA3 ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
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bioRxiv - Microbiology 2023Quote: Whole peripheral blood was collected from newly hatched chicks and total DNA was extracted using the NucleoSpin 96 Blood core kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted from mycelium and conidia with the Nucleospin soil kit for the progeny and with the NucleoBond High Molecular Weight DNA kit (Macherey-Nagel, Düren, Germany) for the parental strain LCP06136 ...
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bioRxiv - Plant Biology 2019Quote: ... genomic DNA from ‘Bartlett.DH’ was purified from young rolled leaves and young meristem tissue using the NucleoSpin Plant II DNA extraction kit (Macherey-Nagel GmbH, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Plasmids were constructed from PCR-generated fragments and pET16b vector cut with restriction enzymes using the isothermal DNA assembly method (48) ...
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bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from ACE-grown cells (DSMZ medium 461 with 5 mM ACE) with the NucleoSpin® Microbial DNA kit (Macherey-Nagel, Düren, Germany) according to the manual and using wide-bore tips ...
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Synthetic Biology 2023Quote: ... Base-editing efficiency when targeting a plasmid-borne gene was estimated by isolating plasmid DNA upon 24-h editing treatment by using the NucleoSpin™ plasmid EasyPure kit (Macherey-Nagel, Dueren, Germany). Either the isolated plasmids or the purified PCR products from independent samples were Sanger-sequenced using the Mix2Seq™ kit (Eurofins Scientific ...
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bioRxiv - Zoology 2024Quote: ... Genomic (g)DNA was extracted and purified using the NucleoSpin®Tissue kit following the manufacturer’s instructions (Macherey-Nagel Gmbh & Co. KG, Düren, Germany). Primers used for the detection of all pathogens are found in Supplementary Data 3.
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bioRxiv - Evolutionary Biology 2020Quote: ... The leaf material was lysed with sterile sand and 7mm glass beads before DNA was extracted with the NucleoMag 96 plant kit (Macherey-Nagel Gmbh & Co., Düren, Germany) using the KingFisher Flex magnetic particle processor (Thermo Scientific) ...
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bioRxiv - Genetics 2022Quote: ... Rubus DNA extractions were performed in 96-well plates from freeze-dried tissue using commercial kits (e.g. Macherey-Nagel Nucleospin kit was used at PFR). Mānuka samples were collected in natural stands in remote locations using silica beads in 2mL screw cap tubes as described in Koot et al ...
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bioRxiv - Plant Biology 2020Quote: ... DNA was extracted using either a MO BIO PowerPlant DNA Isolation Kit (MO BIO Laboratories, Carlsbad, California, USA) or NUCLEOSPIN Plant II Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). Dry plant leaf material (typically ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid preparation and DNA gel extraction were performed using the Nucleospin Plasmid kit and the Nucleospin Extract II kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). DNA sequences were determined by Eurofins-Genomics (Vienna ...
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bioRxiv - Biochemistry 2023Quote: ... and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). In vitro methylation experiments were performed for 10 min (DNA ...
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bioRxiv - Biochemistry 2023Quote: ... and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). In vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc. ...