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Citations for Macherey-Nagel GmbH :
601 - 650 of 724 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The final transgenic BAC DNA is then transferred back to DH10b cells for better extraction yield (Nucleobond BAC100, Macherey-Nagel). DNA is resuspended in injection buffer (10mM Tris-HCL pH 7.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μL water was added and DNA was purified using Nucleospin Gel and PCR Cleanup (Macherey-Nagel, Cat. No 740609.250). Extracted DNA was PCR-amplified to check the insertion of the 3XFLAG tag ...
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bioRxiv - Microbiology 2022Quote: DNA from monolayer cultures as well as from rafts was isolated using the NucleoSpin Blood QuickPure kit (740569.250; Macherey-Nagel) according to the manufacturer’s instructions with some modifications for raft tissues ...
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bioRxiv - Microbiology 2019Quote: ... difficile strains was done using standard methods.33 Routine DNA extractions were performed using the Nucleospin Plasmid Easypure (Macherey-Nagel) and DNeasy Blood and Tissue (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... DNA from the IP and input fractions was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). DNA from IP and input were used for library preparation and sequencing by Illumina Hi-Seq (FASTERIS ...
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bioRxiv - Genetics 2021Quote: DNA extraction from fresh and scale connective tissues was performed using the NucleoSpin® Tissue Kit (Macherey-Nagel, Hoerdt, France), following manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... Collected pellets were stored at −80°C and genomic was DNA isolated using MN Nucleospin Tissue kit (Macherey-Nagel, Germany) according to manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2023Quote: DNA extraction from dried filters was done using NucleoSpin™ Forensic Filter kit (Macherey-Nagel GmbH & Co .KG, Düren – Germany). Filter parts were folded and incubated in 600 μL of lysis buffer T1 at 56 °C for two hours with tube horizontally agitated and then centrifugated 1 min 30 sec at 11,000 g ...
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bioRxiv - Plant Biology 2023Quote: ... Total DNA was extracted using CTAB (Doyle & Doyle 1987) and purified using NucleoSpin® Purification Kit (Macherey-Nagel, Düren, Germany). DNA extractions were profiled in Agarose gel 1% and using Nanodrop ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA of all mcr-positive isolates was extracted using NucleoSpin® Tissue kit (Macherey-Nagel GmbH & Co, Duren, Germany). The libraries were prepared using Nextera XT DNA Sample Preparation Kit and sequenced on MiSeq or NovaSeq 6000 platform (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... all isolates were grown overnight at 20 °C in liquid Zobell medium and bacterial DNA was extracted using the Nucleospin tissue kit following the manufacturer’s instructions (Macherey-Nagel).
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bioRxiv - Biophysics 2023Quote: ... The 600 bp DNA was extracted from the gel using a standard NucleoSpin Gel and PCR cleanup (Macherey-Nagel GmbH). The fluorescence of the doubly labelled 600 bp band was visualized using a Typhoon 9400 scanner (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: We generated high-quality DNA of the expression constructs for electroporations using the NucleoBond Xtra Midi kit (Macherey-Nagel 740410.50)
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bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from the final pellets using the NucleoBond High Molecular Weight DNA Kit according to the manufacturer’s instruction (Macherey-Nagel).
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted from larvae or pupae of the selected lines using NucleoSpin Tissue gDNA extraction kit (Macherey-Nagel).
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bioRxiv - Biophysics 2022Quote: ... Ligated DNA constructs were separated with agarose gels from other reaction products and purified by gel extraction (NucleoSpin, Macherey-Nagel) by avoiding any exposure to ethidium bromide or UV light.
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
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bioRxiv - Plant Biology 2024Quote: DNA-free RNA was isolated with NucleoSpin® RNA Plant and Fungi Kit as recommended by the manufacturer (MACHEREY-NAGEL) and used as template for reverse transcription as previously described (Lemaire-Chamley et al. ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysis and extraction of genomic DNA from dry pellets were performed using the NucleoSpin Tissue XS kit (Macherey-Nagel). Genomic DNA quality and quantity were measured using the NanoDrop (ND-1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1.7 Gb paired-end reads for the RS_LinB were obtained from Red Sea samples preserved in CHAOS buffer (Flot 2007) and extracted using the DNA NucleoSpin kit (Macherey-Nagel) following the protocol recommended by the company ...
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bioRxiv - Systems Biology 2019Quote: ... Histone-DNA complexes were isolated from insoluble nuclear chromatin by centrifugation and DNA was extracted with a NucleoSpin PCR Clean-up kit (Macherey-Nagel). For CUT&RUN quantitative PCR ...
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bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The lysates were then purified to genomic DNA and RNA using NucleoSpin Tissue Kits and NucleoSpin RNA Plus kits (MACHEREY-NAGEL), respectively ...
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bioRxiv - Microbiology 2021Quote: ... 107 cells were inoculated in 50 ml LB medium supplemented with 100 µg/ml spectinomycin and grown overnight at 37 °C for purification of the plasmid library DNA by miniprep (NucleoSpin Plasmid, Macherey-Nagel). The efficiency of fragment insertion (100% ...
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bioRxiv - Plant Biology 2022Quote: 100mg of leaf tissues from the second to fourth fully emerged leaf from the apical bud were used for DNA extraction using the NucleoSpin Plant II kit (Macherey-Nagel). Leaf tissues were disrupted with 400µL PL1 from the kit using BeadBeater (BioSpec ...
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bioRxiv - Genetics 2022Quote: ... cells were scraped from plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel). The resulting plasmid DNA was used for yeast transformation.
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bioRxiv - Genetics 2022Quote: ... the cells were scraped from the plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Synthetic Biology 2022Quote: ... and LA1737 and LA1738 (629 bp downstream of stop codon) (Table S11) from genomic DNA prepared from our Liverpool WT colony using the NucleoSpin Tissue kit (Macherey-Nagel) and ligated into the plasmid sequentially by standard restriction enzyme-based cloning to generate bgcn-Cas9 (AGG1207).
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bioRxiv - Molecular Biology 2019Quote: ... Cells were collected 5 days after transfection and the cell’s genomic DNA was prepared using NucleoSpin Tissue (MACHEREY-NAGEL & Co. KG).
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bioRxiv - Biochemistry 2019Quote: ... the extracted ChIP DNA was further re-purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). The region-specific primer sets used for the ChIP-qPCR analysis have been mentioned in Table S2.
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bioRxiv - Genetics 2019Quote: ... Endotoxin-free plasmid DNA from sequence-verified clones was isolated using the Nucleobond Xtra Midi EF Kit (Macherey-Nagel, Düren Germany), ammonium acetate precipitated and re-suspended in MaxCyte buffer (MaxCyte Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from single legs of individual mosquitoes or whole individual mosquitoes using NucleoSpin DNA Insect Kit (Machery-Nagel) or NucleoSpin Tissue Kit (Macherey-Nagel) following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... primers 4163-4183 and 4288-4308) were amplified by PCR (Rotor Gene 3000, Corbett Research) from 50ng DNA (Nucleospin Tissue kit, Macherey-Nagel). PCR products quantified with Sybr green (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: We extracted both DNA and RNA from the RNAlater samples using the Nucleospin XS Kit in combination with the Nucleospin RNA/DNA Buffer Set (Macherey-Nagel), and we extracted DNA from the ethanol samples using the DNeasy Blood and Tissue kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... flanking Cas9 targeted site was amplified from genomic DNA extracted from wild type MCF10A cells (NucleoSpin tissue extraction kit, Macherey-Nagel), Puro-T2A were amplified from the custom-made plasmid MXS Puro bGHpA using primers containing T2A sequence ...
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bioRxiv - Microbiology 2021Quote: ... selected clones were grown overnight at 37°C under agitation and the plasmid DNA was extracted and purified using the NucleoSpin plasmid Mini or Midi kit (Macherey-Nagel).
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bioRxiv - Genomics 2021Quote: ... genomic DNA was extracted from bacterial strains grown on King’s medium B (King et al. 1954) at 28°C for 24 h using NucleoSpin Microbial DNA kit (Macherey-Nagel, Germany). The quality of the genomic DNA was assessed by electrophoresis in 0.8% agarose gel.
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bioRxiv - Immunology 2022Quote: ... The surviving cells were expanded for 24 h and DNA was extracted using a NucleoSpin Tissue kit (Macherey-Nagel, Cat#740952). An additional eight biological replicates were similarly prepared but did not undergo killing by TC5A87 ...
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bioRxiv - Biochemistry 2022Quote: ... pre-tRNAPheGAA2-2 and human pre-tRNATyrgta8-1 and tRNAArgtct3-2 were amplified by PCR of respective pUC19 vectors and template DNA was isolated using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). RNA substrates were produced by run-off in vitro transcription using T7 RNA polymerase (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Transfection-grade plasmid DNA was isolated from XL10Gold cell overnight culture with the NucleoBond™ Xtra Midi Kit (Macherey-Nagel, Germany).
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bioRxiv - Plant Biology 2022Quote: ... DNA was extracted from the distant rhizosphere using a modified Griffiths’ protocole that co-extracts DNA and RNA (as described above) and from the root and close rhizosphere using NucleoSpin Plant II kit (Macherey-Nagel). DNA was sent for 16S rRNA and ITS sequencing on an Illumina MiSeq in pair-ended mode (2×250 ...
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bioRxiv - Molecular Biology 2023Quote: ... the beads were resuspended in NTI buffer for DNA purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, #740609.250).
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bioRxiv - Microbiology 2023Quote: ... All samples were immediately placed on dry ice and stored at −80 °C until DNA extraction using the NucleoSpin 96 soil kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from leaves using a mixed alkyl trimethylammonium bromide buffer (MATAB) and NucleoMag Plant Kit (Macherey-Nagel, Düren, Germany) as already described by Cormier et al ...
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bioRxiv - Genomics 2023Quote: ... Transformed cells were grown in Kp medium for 8-10 vegetative divisions and their genomic DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel). Transgene injection levels (copy number per haploid genome ...
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bioRxiv - Genomics 2023Quote: The genomic DNA was extracted from at least one million cells (typically 3 million, coverage 300x) using the NucleoSpin Tissue kit (Macherey-Nagel). To enrich for GBCs ...
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bioRxiv - Evolutionary Biology 2023Quote: Whole DNA was extracted from fecal samples following the optimized protocol of (79) using the Genomic DNA from soil kit (NucleoSpin, Macherey-Nagel). Two successive extractions were done and a purification step was added to retrieve high molecular weight DNA suitable for long-read sequencing (79) ...