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Citations for Macherey-Nagel GmbH :
401 - 450 of 724 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the DNA plasmid was extracted using a NucleoBond® Xtra Midi kit (MACHEREY-NAGEL #740410.50). The efficiency of transformation was controlled by plating 0.01% of the transformation reaction to a 15 cm LB agar plate with 100 µg/mL ampicillin.
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bioRxiv - Bioengineering 2020Quote: ... DNA was extracted with the NucleoSpin®Soil Kit (MACHEREY-NAGEL GmbH & Co. KG, Germany) using buffer SL2 and enhancer solution ...
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bioRxiv - Zoology 2020Quote: ... DNA was extracted following the standard protocol of the NucleoSpin® Tissue Kit (Macherey-Nagel) except that we performed two elution steps ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA (gDNA) was extracted from the pellets using the NucleoSpin tissue kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Transformants genomic DNA was extracted with the NucleoSpin® Tissue kit (Macherey-Nagel, Düren, Germany) and further analyzed by (i ...
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bioRxiv - Cell Biology 2019Quote: ... genomic DNA (gDNA) of transfected parasites was isolated using the NucleoSpin Blood Kit (MACHEREY-NAGEL) according to the manufacture’s protocol and used as template in diagnostic PCR ...
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bioRxiv - Plant Biology 2019Quote: ... Total DNA was extracted with the NucleoSpin® Plant II kit (Macherey-Nagel, Düren, Germany) or the DNeasy Plant Mini Kit (QIAGEN Biotecnologia Brasil Ltda ...
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bioRxiv - Microbiology 2021Quote: ... After sequence verification plasmids were isolated using NucleoBond Xtra Midi plasmid DNA purification (Macherey-Nagel). For recombinant antibody expression ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted from the cell pellets using the NucleoBond AXG20 kit (Macherey-Nagel), and DNA purity and concentration were determined with the Nanodrop 2000c spectrophotometer (Thermoscientific).
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bioRxiv - Immunology 2020Quote: ... DNA was purified by using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL). The transposed DNA was amplified by PCR for 10-12 cycles by using the Nextera DNA Library Preparation Kit and Nextera XT Indexing Kit (Illumina) ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA was extracted with the NucleoSpin Tissue kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and libraries prepared using the ONT Rapid Kit (SQK-RBK004 ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from cell pellets using the NucleoSpin Blood XL Kit (Macherey-Nagel). The sgRNA sequence was amplified by PCR with sufficient gDNA to maintain representation ...
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bioRxiv - Microbiology 2022Quote: ... Intracellular and supernatant HBV DNAs were isolated using the Nucleospin Tissue extraction kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA was extracted from pelleted cells using minipreps (NucleoSpin Plasmid EasyPure Kit - Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Genomic DNA was extracted from each founder male using the NucleoSpin Tissue kit (Macherey-Nagel) and subjected to two separate PCR reactions with primers LA2750 ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was extracted and purified using NucleoBond AXG and Buffer Set III (MACHEREY-NAGEL) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction of soil samples was performed using the NucleoSpin kit (MACHEREY-NAGEL, Düren, Germany) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with a commercial gel and PCR clean-up kit (Macherey-Nagel, Germany) and was directly used for quantitative PCR with SimpleChIP human CDKN1A promoter primers (Cell Signaling Technology ...
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bioRxiv - Genomics 2023Quote: DNA samples were prepared from ≤1,000 autogamous cells using the NucleoSpin Tissue kit (Macherey-Nagel). For PCR analysis of IES excision ...
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bioRxiv - Microbiology 2022Quote: DNA extraction was performed as described in [21] using the Nucleospin tissue kit (Macherey-Nagel). DNA concentration and purity were checked with a NanoDrop One (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, 740609) and quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted from 570 foot tissue samples using NucleoSpin® Tissue kits (Macherey-Nagel) or a CTAB protocol (Doyle & Dickson ...
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bioRxiv - Genomics 2023Quote: DNA extractions were carried out using the NucleoSpin Plant II kit (Macherey-Nagel, GmbH, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA and RNA was extracted using the NucleoMag VET extraction kit (Macherey-Nagel, Germany) as described by the manufacturer’s instructions with the IDEALTM 96 extraction robot (Innovative Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the plasmid DNA was amplified using a NucleoBond Xtra Midi kit (Cat.#740410; Macherey-Nagel) and the sequence was verified with Sanger sequencing (GENEWIZ).
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bioRxiv - Microbiology 2024Quote: ... To recover the DNA a Nucleo Spin Gel and PCR Clean up kit (Macherey-Nagel) was used and the DNA was eluted in 20 µl TE buffer (10 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA and mRNA were extracted using NucleoSpin Tissue and NucleoSpin RNA kits (Macherey-Nagel), respectively.
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA were isolated from cell lines using the NucleoSpin Tissue extraction kit (Macherey-Nagel). Genomic DNA amount and quality were assessed using a NanoDrop (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was purified using Macherey-Nagel NucleoSpin Blood XL Maxi Kit (740950.50, Macherey-Nagel) and prepared as previously described [Kampmann et al 2014] with modifications ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 12,000 ×g at 4 °C for 20 minutes and the cleared supernatant was either directly used for the reconstitution assays or subjected to purification with Ni-NTA agarose (Macherey-Nagel). The suspension was incubated for 60 minutes and transferred to a fretted column ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Immunology 2022Quote: Total RNA from cultured cells was extracted using the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, Germany). RNA was retrotranscribed using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The protein samples resolved in agarose gel were vacuum-transferred onto the nitrocellulose membrain (Porablot, MACHEREY-NAGEL), and subjected to a standard Western-Blotting procedure with either anti-Sup35NM ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Biochemistry 2024Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Genomics 2020Quote: Genomic DNA from cells and tissues was isolated with the NucleoSpin® Tissue Kit (Macherey-Nagel), and from blood (150 µl ...
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bioRxiv - Microbiology 2019Quote: ... mutant BAC DNAs were purified from E.coli using the Nucleobond BAC100 kit (Macherey-Nagel, Düren, Germany). The day before transfection ...
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bioRxiv - Microbiology 2021Quote: Procaryotic DNA from the pellet was isolated using the MN NucleoSpin Soil Kit (Macherey-Nagel, Germany) and a Precellus 24 homogenizer (Bertin ...
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bioRxiv - Microbiology 2021Quote: Procaryotic DNA from the pellet was isolated using the MN NucleoSpin Soil Kit (Macherey-Nagel, Germany) and a Precellus 24 homogenizer (Bertin ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was isolated from DH5α using the Nucleospin Plasmid kit (MACHEREY-NAGEL Inc., Bethlehem, PA) and transformed into the DAP-auxotroph donor strain E ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial genomic DNA was extracted from faeces by using NucleoSpin 96 Soil kits (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... amplified DNA was purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel) as per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Genomic DNA from the cell pellets was purified using the NucleoSpin blood L kits (Macherey-Nagel) according to the manufacturer’s instructions and was quantified using PicoGreen dsDNA assay kits (ThermoFisher).
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bioRxiv - Genomics 2019Quote: ... genomic DNA was extracted from a single adult female using the NucleoSpin Tissue XS (Macherey-Nagel) according to manufacturer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Further purification of the extracted DNA was done with NucleoSpin® gDNA Clean-up (Macherey-Nagel). For laboratory bred F2 offspring of the two-generation acclimation experiment ...