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Citations for Macherey-Nagel GmbH :
551 - 595 of 595 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Cleaved Histone-DNA complexes were isolated by centrifugation and DNA was extracted with a NucleoSpin PCR Clean-up kit (Macherey-Nagel, 740609).
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: The final PCR amplified library was submitted to two consecutive 1× NucleoMag NGS Clean-up and Size select magnetic bead selections (Macherey-Nagel – 7449970.5) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... exonuclease V was used to digest the linear DNA in the sample prior to isolation of the residual putative cirDNA using a DNA clean-up kit (NucleoSpin gel and PCR clean-up kit, Macherey-Nagel, Germany). The final cirDNA preparation was dissolved in TE buffer ...
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bioRxiv - Genetics 2023Quote: ... was added for 30 min at 37°C and DNA was purified using NucleoSpin Gel and PCR Clean-Up columns (Macherey-Nagel, #740609.250) according to the manufacturer’s instructions ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... RNA bands according to the size of the 18s and 28s rRNA were cut from the agarose gel and extraction was performed using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel). The extraction was performed according to the manufactureŕs protocol for RNA extractions from agarose gels using the buffer NTC (Macherey Nagel).
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplicons were purified using the NucleoSpin gel and PCR cleanup kit following the manufacturer’s instructions (Macherey-Nagel Inc., Bethlehem, PA) and submitted for Sanger sequencing with the same primers used to generate the amplicons ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reaction was performed on the new synthetized vector to amplify the HA1-DCK*-P2A-GFP-HA2 sequence corresponding to the HDR (homology directed repair) DNA template and was purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel # 740609.10) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of the sample with gRNA–TAR and –p24 combined was run on a 1% agarose gel containing ethidium bromide and the two expected bands were cut out separately and purified using the Nucleospin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Developmental Biology 2022Quote: ... plasmids were linearized by AscI (pBSK vector) or SfiI (pYX vector) and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, #740609). cRNA was produced by in vitro transcription using mMessage mMachine™ T3 kit (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was amplified by PCR methods using the following oligo-DNA primers and the products were purified with micro spin columns (MACHEREY-NAGEL, 74060910). Each reverse primer also possesses T3 sequence for transcription.
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Spirolactone, an unprecedented antifungal β-lactone spiroketal macrolide from Streptomyces iranensisbioRxiv - Biochemistry 2024Quote: ... DNA purification was conducted on 1% Tris-acetate-EDTA (TAE) agarose gel followed by using NucleoSpin Gel and PCR Clean-up Kits (Macherey-Nagel, Germany). One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E ...
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bioRxiv - Microbiology 2021Quote: ... was linearized by AdeI restriction enzyme digestion and DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). For in-vitro transcription ...
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bioRxiv - Microbiology 2021Quote: The qPCR product for hsv1-miR-H27 was first subjected to agarose gel electrophoresis and the band complementary to the size of cDNA was excised and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Germany). The purified product was cloned into T&A™ cloning vector by using T&A™ Cloning Vector (Yeastern Biotech Co. ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were analysed using 1.5% agarose gel electrophoresis in 1x Tris-borate-EDTA (TBE) buffer and purified from the gel using a NucleoSpin® gel and PCR clean-up kit (Macherey-Nagel, Germany).
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bioRxiv - Physiology 2021Quote: ... Ak1 cDNA and plasmids were digested with SpeI and BglII and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Ligation was performed using Ligation High Ver ...
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bioRxiv - Immunology 2020Quote: ... The reactions were then run at 100V in a 1% agarose gel and the digest vectors were excised and purified using a NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, USA) according to instructions set by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA was isolated with NucleoSpin Plasmid Mini Kit and extracted from an agarose gel with NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). All constructs were verified by restriction digest and Sanger sequencing ...
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bioRxiv - Genomics 2022Quote: ... The DNA band at 5 to 10kb size was collected for extraction with a NucleoSpin Gel and PCR Clean-up purification kit following the manufacturer’s instructions (Macherey-Nagel, cat# 740609.250, Germany), and was eluted with 15 μl NF water.
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were run on gel electrophoresis before gel extraction of bands of interest (with sizes corresponding to either a wild-type allele or a deleted allele) (Nucleospin gel and PCR clean-up, Macherey-Nagel Cat# 740609.50). Fragments were cloned into blunt-end TOPO vector (Invitrogen Cat# 45024) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cat#M0491S/L) and gel extractions were done with the Macherey-Nagel NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Cat#740609). DNA assemblies were carried out by In-Fusion Cloning (Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: ... the rhizosphere and root subpools were purified on an agarose gel (amplicon ∼450 bp) using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany), whereas all other subpools were purified with CleanNGS beads ...
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bioRxiv - Cell Biology 2023Quote: ... The amplicons were resolved by agarose electrophoresis and extracted from the gel using NucleoSpin Gel and PCR Clean-up XS (Macherey-Nagel, Düren, Germany). The extracted DNA was then processed into libraries for sequencing using Ion Plus Fragment Library Kit and Ion Xpress Barcode Adapters 1-16 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was isolated with NucleoSpin Plasmid Mini Kit and extracted from an agarose gel with NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel, Duren, Germany). All constructs were verified by restriction digest and Sanger sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments encoding the open reading frame (ORF) of the nanobodies with 15-bp complementary overhangs were amplified using PCR and purified from agarose gel bands (Macherey-Nagel Ref 740609.50). A pAAV vector ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products (with approximately 500-600 bp in length) were then purified with NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, MN) or with NZYGelpure kit (NZYtech ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissue using a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers suggested by the stock facility (forward ...
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bioRxiv - Systems Biology 2020Quote: ... with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Plasmids were constructed from PCR-generated fragments and pET16b vector cut with restriction enzymes using the isothermal DNA assembly method (48) ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR product from the 5’ RACE procedure was excised from a 1% agarose gel and purified by column capture (Macherey-Nagel Nucleospin mini kit). The purified product was then Sanger sequenced (Eurofins Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissues with a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers (forward 5’ to 3’ ...
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Microbiology 2022Quote: ... The product of barcoding qPCR was cleaned by gel extraction using NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL, GmbH & Co, KG, Germany), after 1% AGE electrophoresis run for 1 hour at 100 V ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and bands of the correct size were excised and cleaned up with a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Cat. No. 740609.5) followed by cleanup with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2024Quote: ... PCRs for loci of interest were run using Amplicon-EZ (Genewiz) partial IlluminaÒ adapters and amplicons were processed using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Catalog no. 740609.250). Paired end (2 x 250 bp ...
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bioRxiv - Microbiology 2024Quote: The PCR product was run on a 0.8% agarose gel and a fragment of the correct size was excised for clean up using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Ref. No. 740609.5) followed by Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products were isolated and purified by the Gel clean up guidelines of the NucleoSpin Gel and PCR clean-up Kit (Macherey-Nagel, Ref. No. 740609.50).
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bioRxiv - Synthetic Biology 2024Quote: ... The gel purifications were performed using the Freeze N Squeeze DNA Gel Extraction Spin Columns (BIO-RAD, CAT#: 4106139 and NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL, CAT#: 740609.250).
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bioRxiv - Developmental Biology 2024Quote: ... dsDNA repair templates were amplified by PCR using primers with containing 5′ SP9 modifications (IDT) and purified using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.250) prior to injections ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These were obtained by using as template a 1:10 serial dilution (8 dilutions) of a cleaned (Macherey-Nagel’s NucleoSpin Gel and PCR Clean-up) and quantified (Invitrogen’s Qubit™ ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplified regions of CRISPR-C unedited samples and the specific bands derived from the amplification of the deletion scar and circular junction sites were dissected and DNA products purified by NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL Düren, Nordrhein-Westfalen, Germany) and sent for Sanger sequencing at Eurofins Genomics ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated on 2.5% agarose gels and extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany, cat. no. 740609). The purified PCR products were then cloned using a Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Spheroids were transferred into PCR-clean 1.5 mL microtubes with 100 µL of lysis buffer (LB1 from Macherey-Nagel or 700 µL of Buffer RLT (79216, Qiagen). Lysates were snap-frozen and stored at −80 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Amplification of respective gene fragments was verified by Sanger sequencing of the amplicons after separation by agarose gel electrophoresis and purification (NucleoSpin® Gel and PCR Clean-up kit, Macherey-Nagel GmbH & Co. KG, Düren, Germany). Individual plants were propagated to obtain homozygous mutants and wildtypes and were phenotyped for EPH ...