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Citations for Macherey-Nagel GmbH :
451 - 500 of 595 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and DNA bands of interest were isolated using the NucleoSpin® Gel & PCR Cleanup Kit from Macherey-Nagel. The extracted DNA underwent Sanger sequencing at Eurofins Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The products were inspected on agarose gel and purified using Nucleofast PCR purification plates (Macherey-Nagel, Dueren, Germa) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Cell Biology 2024Quote: ... Enzyme-resistant dbDNA was then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). When 18 mol of plasmid template is reacted and subsequently eluted in 30 µL of sterile MQ ...
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bioRxiv - Genomics 2024Quote: TempO-LINC libraries were purified using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, cat# 740609.250), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons were then purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH, Cat. # 740609.50) and sequenced through Sanger sequencing (Microsynth Seqlab GmbH).
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bioRxiv - Cell Biology 2020Quote: ... Each worm pellet was re-suspended in 1M DTT and RA1 (solution from the NucleoSpin® RNA kit (Macherey-Nagel, Duren Germany #740955.50)) and frozen at −80°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... and J321 was performed at the CDC according to a Nabsys solution-based protocol modified from the bacterial DNA protocol for AXG 20 columns and Nucleobond Buffer Set III (Macherey-Nagel, Bethlehem, PA). Purified DNA was sent to Nabsys for nicking ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplicons were purified using the NucleoSpin® Gel and PCR clean up kits from Macherey-Nagel (Bethlehem, PA), after which quantification and qualification of purified band(s ...
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bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitated DNA was purified with NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Digested DNA was cleared from restriction enzymes using the NucleoSpin Gel and PCR Clean-up kit (740609.250, Macherey-Nagel). Insert-DNA (50 ng ...
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bioRxiv - Biochemistry 2020Quote: ... After gel extraction of the DNA fragments with the PCR Clean-up Gel Extraction Kit (Macherey-Nagel, Düren, Germany), the DNA fragments and the vector DNA were digested by the same restriction enzymes (Supplemental Table S4 ...
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bioRxiv - Microbiology 2020Quote: ... ChIP-DNA and input DNA were purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel). The DNA concentration of the input DNA was determined using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... A total of 400 µl of this PCR was run and purified using column-based clean-up (Macherey-Nagel).
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bioRxiv - Biochemistry 2021Quote: ... the band containing the efrCD genes were extracted with the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel).
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bioRxiv - Molecular Biology 2021Quote: ... DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. Genomic DNA extractions from Amycolatopsis and E ...
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bioRxiv - Microbiology 2020Quote: ... difficile strains was estimated by quantitative PCR on genomic DNA extracted using the NucleoSpin Microbial DNA kit (Macherey-Nagel). The total chromosome copy number was quantified based on the reference gene dnaF (CD1305 ...
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bioRxiv - Molecular Biology 2023Quote: ... Five tubes of each amplicon were pooled and DNA purified using NucleoSpin Gel and PCR Clean-Up (Macherey-Nagel). DNA was eluted in 20 µl of Elution Buffer and concentration was measured using Qubit 3.0 fluorometer (Life Technologies).
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bioRxiv - Microbiology 2024Quote: ... All positive samples were purified with the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Each mix was then purified using the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). DNA was then eluted and quantified at a Nanodrop 2000 Spectrophotometer (ThermoScientific) ...
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bioRxiv - Neuroscience 2024Quote: ... the bands of interest were excised and purified with the Gel and PCR clean up (ref 740609, Macherey-Nagel) with a final elution volume of 20 µl ...
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bioRxiv - Cell Biology 2024Quote: ... the first-strand cDNA was purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel, no. 470609) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... and linearized plasmid was purified from the gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). In-Fusion reaction was prepared as follows ...
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bioRxiv - Immunology 2023Quote: ... The excised DNA fragments were further refined using nucleospin gel and by using PCR clean-up kit (Macherey-Nagel) and quantified for determining the purity and concentration ...
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bioRxiv - Physiology 2024Quote: ... Target bands were excised and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel GmbH & Co. KG). The purified DNA was subsequently sequenced using the Sanger method to verify the specificity of the amplified fragments.
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bioRxiv - Microbiology 2024Quote: ... The gRNA cassette was isolated by gel electrophoresis with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and the DNA was then amplified by PCR using primers with Illumina sequencing adapters ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated by agarose gel electrophoresis and purified from agarose gels using the NucleoSpin kit (Macherey-Nagel). Purified PCR products were subcloned into linearized plasmid vectors of interest using the In-fusion cloning system following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and the resulting digested fragment was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, 740609.250). Guide RNAs were designed using the CRISPOR tool (http://crispor.gi.ucsc.edu/ ...
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bioRxiv - Cancer Biology 2020Quote: ... treatment with 100µg/ml RNase A (37° for 15 minutes) and 50µg/ml proteinase K and purification using the PCR cleanup kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected for 400-550 bp using a 2% agarose/TAE gel and column purification (Macherey-Nagel). Libraries were sequenced as above ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μL water was added and DNA was purified using Nucleospin Gel and PCR Cleanup (Macherey-Nagel, Cat. No 740609.250). Extracted DNA was PCR-amplified to check the insertion of the 3XFLAG tag ...
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bioRxiv - Immunology 2020Quote: ... Positive clones were identified by colony PCR and the plasmid was purified using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel). The cloned devil NLRC5 transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
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bioRxiv - Immunology 2020Quote: ... amplicons were separated by gel electrophoresis and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Library quantification and quality control was performed using Qubit (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNAs have been extracted on an agarose gel and purified (using the PCR clean-up gel extraction kit #740609.50, Macherey-Nagel) and the sanger sequencings have been performed by Eurofins ...
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bioRxiv - Genetics 2020Quote: ... Obtained libraries were pooled in equimolar amounts and purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), followed by a second purification step of gel extraction and size exclusion with AMPure XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Biophysics 2023Quote: ... The 600 bp DNA was extracted from the gel using a standard NucleoSpin Gel and PCR cleanup (Macherey-Nagel GmbH). The fluorescence of the doubly labelled 600 bp band was visualized using a Typhoon 9400 scanner (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Digestion products were isolated on agarose gels and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Plasmid isolation was performed with NucleoSpin Plasmid kit (Macherey-Nagel) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The 140-bp UPTAG and DNTAG libraries were purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel), quantified using the Qubit dsDNA HS kit (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... NucleoSpin® plasmid isolation kit and NucleoSpin® gel and PCR clean-up kit were purchased from Macherey-Nagel (Germany). PierceTM protease inhibitor tablets EDTA free and Pierce™ high capacity Ni-IMAC resin were purchased from Thermo Scientific (international) ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were purified by the NucleoSpin Gel and PCR cleanup kit according to manufacturer’s instructions (Macherey-Nagel, Dueren, Germany). Purified products were sent for Sanger sequencing with primers rbcL404+ and rbcL587-to Macrogen (http://dna.macrogen-europe.com).
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bioRxiv - Genetics 2024Quote: ... We purified the resulting fragment from a 2% agarose gel using the NucleoSpin Gel and PCR Purification Kit (Macherey-Nagel). We amplified the 1483bp RTFDC1 promoter sequence off of a promoter plasmid (Genecopoeia HPRM41362-PF02 ...
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bioRxiv - Immunology 2024Quote: ... PCR was migrated in a 1.3% agarose gel and amplicons were cut and purified using a gel and PCR clean-up kit (Macherey-Nagel) for further DNA sequencing ...
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bioRxiv - Cell Biology 2024Quote: mRNAs were quantified in mouse kidneys and cultured cells by quantitative RT-PCR RNA extraction performed as described in the manufacturer’s protocol (NucleoSpin RNA, Macherey-Nagel). cDNA was synthesized with the High-Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription products were purified by NucleoSpin Gel and PCR clean-up kit equilibrated with NTC buffer (Macherey-Nagel). Dideoxy DNA sequencing samples were prepared using Thermo Sequenase DNA polymerase (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Genomics 2020Quote: ... for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Immunology 2020Quote: ... The amplified products were identified by gel electrophoresis and purified using Nucleospin PCR and Gel Clean Up Kit (Macherey-Nagel # 740609.5). Alternatively ...