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Citations for Bimake :
201 - 250 of 258 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Nphp1 cDNA fragments between exons 2 and 11 were PCR-amplified using Universe High-Fidelity Hot Start DNA polymerase (Bimake) and two primers (F1-mNPHP1-RT and R1-mNPHP1-RT ...
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bioRxiv - Developmental Biology 2021Quote: ... 2X SYBR Green PCR Master Mix was purchased from Bimake. Real-time quantitative PCR was conducted using the QuantStudionTM 7 flex System (Applied Biosytstems) ...
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bioRxiv - Developmental Biology 2021Quote: ... the supernatants were collected and incubated with anti HA magnetic beads (Bimake) or IgG bound protein A/G magnetic beads (Bimake ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% [mass/vol] sodium dodecyl sulfate) containing 1X protease inhibitor cocktail (Bimake) for 2 hours on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... or IgG bound protein A/G magnetic beads (Bimake) followed by an overnight rotation at 4oC ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time PCR was performed on a QuantStudio 3 Real-time PCR system using gene-specific primers (Table S2) and 2x SYBR Green qPCR Master Mix (Bimake). The amplification conditions were 40 cycles of 95°C for 10 s and 60°C for 30 s ...
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bioRxiv - Cancer Biology 2021Quote: ... WTS-8 (Bimake, Houston, TX), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Total proteins were extracted using the RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China) containing 1×complete proteinase inhibitor (Bimake), and protein concentration was defined using BCA protein assay kit (Beyotime) ...
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bioRxiv - Genomics 2021Quote: ... and 0.5% Nonidet P-40 and aprotease and phosphatase inhibitor cocktail (Bimake, Shanghai, China). Equal amounts of protein were separated by 10% SDS-PAGE gel and transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... and supplemented with EDTA-free protease inhibitor cocktail (Bimake). Cells were lysed by dounce homogenization and complex formation was initiated in the presence of 10 μg/mL Nb35 ...
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bioRxiv - Biophysics 2021Quote: ... and supplemented with EDTA-free protease inhibitor cocktail (Bimake). Cells were lysed by dounce homogenization and complex formation was initiated with the addition of 10 μg/mL Nb35 ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Triton X-100) supplemented with Protease Inhibitor Cocktail (Bimake). Homogenates were centrifuged at 20,000 xg for 15 min at 4 °C to remove insoluble materials ...
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bioRxiv - Immunology 2020Quote: ... or rabbit IgG was cross-linked to protein A/G magnetic beads (Bimake cat. B23202) using dimethyl pimelimidate (ThermoFisher Scientific cat ...
Product cited:
Cat# B23202, SKU# B23202-5 ml, 5 ml, $306.00 Ask -
bioRxiv - Immunology 2020Quote: ... nuclear and cytoplasmic extracts from HaCaT cells were prepared with a Nuclear Protein Extraction Kit (Beyotime cat. P0027) supplemented with a protease inhibitor cocktail (Bimake cat. B14001). The lysates were incubated with anti-PPP6C (Abcam cat ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Immunology 2020Quote: ... The cDNA was mixed with 2 × SYBR Green qPCR Master Mix (B21202, Bimake, Houston, TX, USA), and the real-time PCR reaction was performed on the 7500 Real-Time PCR System (ABI ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21202, SKU# B21202-5 mL, 5 mL, $137.00 Ask -
bioRxiv - Bioengineering 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on ice ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GAPDH (Bimake, A5028), mouse anti-GAPDH (Proteintech ...
Product cited:
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bioRxiv - Genetics 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on a rotator at 4°C ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Genomics 2020Quote: ... before reverse transcription using All-in-One cDNA Synthesis SuperMix (Bimake, Houston, TX, USA). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was reverse transcribed into cDNA (Bimake, Houston, TX, USA), and real-time PCR was performed (LightCycler96 ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... 0.1% SDS) plus protease inhibitor cocktail (Bimake, B14001) 48 h after transfection ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Genetics 2020Quote: ... and phosphatase inhibitor cocktail (Bimake, B15001). Following centrifugation at 12,000 rpm at 4°C for 15 min ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... protein A-agarose beads (Santa Cruz, sc-2001; Bimake, B23202) were used ...
Product cited:
Cat# B23202, SKU# B23202-5 ml, 5 ml, $306.00 Ask -
bioRxiv - Genetics 2020Quote: ... EDTA-free protein inhibitor cocktail was purchased from Bimake (B14001). Polyethylenimine (PEI ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... the reverse transcription (RT) reactions were performed using All-in-One cDNA Synthesis SuperMix kit (Bimake). The cDNA was then used for real-time qPCR using 2x SYBR Green qPCR Master Mix (Bimake ...
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bioRxiv - Molecular Biology 2020Quote: ... or anti-Myc magnetic beads (B26201, Bimake). Briefly ...
Product cited:
Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high...Cat# B26201, SKU# B26201-1 mL, 1 mL, $319.00 Ask -
bioRxiv - Genetics 2020Quote: ... 1% Nonidet P-40) supplemented with protease inhibitor cocktail (Bimake B14001) for 1 hour on a rotator at 4°C ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... or HA magnetic beads (Bimake) were mixed with cell lysate at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... Quantitative PCR (qPCR) was performed using the ABI Real-Time PCR Detection System with SYBR Green qPCR Master Mix (Bimake). Data were analyzed using DART-PCR70 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM tris(2-carboxyethyl)phosphine(TCEP) 10% (v/v) glycerol with additional EDTA-free Protease Inhibitor Cocktail (Bimake), and then incubated with agitation for 20 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% Triton X-100) containing a cocktail of protease and phosphatase inhibitors (Bimake, Houston, TX), sonicated (3 × 45 s in a Sonic Dismembrator apparatus ...
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bioRxiv - Genomics 2020Quote: ... and ligated by Quick-Fusion cloning (Bimake) into pMEregΔalpA digested with PmlI and BamHI to remove the part containing lacIq ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then used for real-time qPCR using 2x SYBR Green qPCR Master Mix (Bimake) with the QuantStudio™ 6 Flex Real-Time PCR system (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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bioRxiv - Biochemistry 2020Quote: ... RqcH-GS-FLAG3 was eluted by addition of 200 μL opening buffer containing 0.1 mg/mL poly-FLAG peptide (Biotool, Bimake) for 45 min on a turning wheel ...
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bioRxiv - Microbiology 2020Quote: ... or with Anti-Flag magnetic beads (Bimake), anti-Myc magnetic beads (Bimake) ...
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bioRxiv - Microbiology 2020Quote: ... and the anti-Flag magnetic beads were purchased from Bimake. 2’3’-cGAMP was purchased from Invivogen ...
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bioRxiv - Microbiology 2020Quote: ... anti-Myc magnetic beads (Bimake). After incubation overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... using the 2x SYBR Green qPCR Master Mix (Bimake, Houston, TX, USA) and segment 7 specific primers (BTV1 S7/322F ...
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bioRxiv - Cancer Biology 2020Quote: ... and Nickel Magnetic Beads for His tag protein purification (Bimake, B23602) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant proteins were purified using GSH Magnetic Beads for GST tag protein purification (Bimake, B23702) and Nickel Magnetic Beads for His tag protein purification (Bimake ...
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bioRxiv - Cancer Biology 2020Quote: ... cell lysates were incubated with antibody-Flag or antibody-Myc affinity gel (Bimake) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were performed using the Mouse Direct PCR Kit (Bimake). Primers Ddi2F and Ddi2R were used for genotyping of Ddi2PD strain samples ...
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bioRxiv - Biochemistry 2020Quote: ... with 1X protease inhibitor cocktail (Bimake) and 50 µM PR-619 (non-specific DUB inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with 10 μL CCK8 reagent (cell counting kit-8, Bimake) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μL of 1:10 diluted cDNA was added to 10 μL of 2× SYBR® Green Supermix (Bimake, Houston, Texas, USA) and the final concentration of each primer was 250 nM in 20 μL of total volume ...
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bioRxiv - Neuroscience 2020Quote: ... Real-time polymerase chain reaction was carried out in duplicate using 2× SYBR® Green Supermix (Bimake, Houston, Texas, USA), and reactions were run on a CFX Connect Real-Time PCR Detection System (BioRad Laboratories ...
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bioRxiv - Pathology 2020Quote: ... RT-PCR was performed with SYBR Green qPCR Master Mix reagent (Bimake, Houston, TX, USA) in a 7300 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Pathology 2020Quote: ... The cDNA samples were amplified by Real-time quantitative PCR (RT-qPCR) using SYBR Green qPCR Master Mix (Bimake) and 18S was used as a standard reference ...
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bioRxiv - Genetics 2020Quote: All mice were genotyped with the tail DNA which was extracted using the Mouse Direct PCR Kit (Bimake). Briefly ...