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Citations for Bimake :
201 - 249 of 249 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM tris(2-carboxyethyl)phosphine(TCEP) 10% (v/v) glycerol with additional EDTA-free Protease Inhibitor Cocktail (Bimake), and then incubated with agitation for 20 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% Triton X-100) containing a cocktail of protease and phosphatase inhibitors (Bimake, Houston, TX), sonicated (3 × 45 s in a Sonic Dismembrator apparatus ...
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bioRxiv - Genomics 2020Quote: ... and ligated by Quick-Fusion cloning (Bimake) into pMEregΔalpA digested with PmlI and BamHI to remove the part containing lacIq ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then used for real-time qPCR using 2x SYBR Green qPCR Master Mix (Bimake) with the QuantStudio™ 6 Flex Real-Time PCR system (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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bioRxiv - Biochemistry 2020Quote: ... RqcH-GS-FLAG3 was eluted by addition of 200 μL opening buffer containing 0.1 mg/mL poly-FLAG peptide (Biotool, Bimake) for 45 min on a turning wheel ...
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bioRxiv - Microbiology 2020Quote: ... or with Anti-Flag magnetic beads (Bimake), anti-Myc magnetic beads (Bimake) ...
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bioRxiv - Microbiology 2020Quote: ... and the anti-Flag magnetic beads were purchased from Bimake. 2’3’-cGAMP was purchased from Invivogen ...
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bioRxiv - Microbiology 2020Quote: ... anti-Myc magnetic beads (Bimake). After incubation overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... using the 2x SYBR Green qPCR Master Mix (Bimake, Houston, TX, USA) and segment 7 specific primers (BTV1 S7/322F ...
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bioRxiv - Cancer Biology 2020Quote: ... and Nickel Magnetic Beads for His tag protein purification (Bimake, B23602) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant proteins were purified using GSH Magnetic Beads for GST tag protein purification (Bimake, B23702) and Nickel Magnetic Beads for His tag protein purification (Bimake ...
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bioRxiv - Cancer Biology 2020Quote: ... cell lysates were incubated with antibody-Flag or antibody-Myc affinity gel (Bimake) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were performed using the Mouse Direct PCR Kit (Bimake). Primers Ddi2F and Ddi2R were used for genotyping of Ddi2PD strain samples ...
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bioRxiv - Biochemistry 2020Quote: ... with 1X protease inhibitor cocktail (Bimake) and 50 µM PR-619 (non-specific DUB inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with 10 μL CCK8 reagent (cell counting kit-8, Bimake) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μL of 1:10 diluted cDNA was added to 10 μL of 2× SYBR® Green Supermix (Bimake, Houston, Texas, USA) and the final concentration of each primer was 250 nM in 20 μL of total volume ...
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bioRxiv - Neuroscience 2020Quote: ... Real-time polymerase chain reaction was carried out in duplicate using 2× SYBR® Green Supermix (Bimake, Houston, Texas, USA), and reactions were run on a CFX Connect Real-Time PCR Detection System (BioRad Laboratories ...
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bioRxiv - Pathology 2020Quote: ... RT-PCR was performed with SYBR Green qPCR Master Mix reagent (Bimake, Houston, TX, USA) in a 7300 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Pathology 2020Quote: ... The cDNA samples were amplified by Real-time quantitative PCR (RT-qPCR) using SYBR Green qPCR Master Mix (Bimake) and 18S was used as a standard reference ...
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bioRxiv - Genetics 2020Quote: All mice were genotyped with the tail DNA which was extracted using the Mouse Direct PCR Kit (Bimake). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell viability was tested by using Cell Counting Kit-8 (CCK-8, Bimake, B34302). The figures were plotted from viral RNA copies in supernatants versus the drug concentrations using GraphPad Prism 6 software.
Product cited:
The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell...Cat# B34302, SKU# B34302-5 mL (500 rxns), 5 mL (500 rxns), $71.00 Ask -
bioRxiv - Cancer Biology 2020Quote: ... and phosphatase inhibitors (Bimake) and determined the protein concentration though BCA protein assay kit (Beyotime Biotechnology) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100) with EDTA-free protease inhibitors (Bimake) and phosphatase inhibitors (Bimake ...
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bioRxiv - Cell Biology 2020Quote: ... Inhibitors were tested for toxicity using a tetrazolium-based Cell Counting Kit-8 (Bimake, Houston, TX) at working concentrations for 12 hrs ...
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bioRxiv - Microbiology 2020Quote: ... the primers prW741 and prW742 and SYBR qPCR mastermix (Bimake). The primers prW83 and prW84 for GAPDH and prW819 and prW820 for c-myc were used as references/controls ...
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bioRxiv - Immunology 2020Quote: ... anti-Flag magnetic beads (Bimake, USA), or anti-Myc magnetic beads (Bimake ...
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bioRxiv - Immunology 2020Quote: ... and the anti-Flag magnetic beads were from Bimake (USA). Alexa Fluor 488 goat anti-rabbit IgG secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... or anti-Myc magnetic beads (Bimake, USA). After incubation overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... pH 7.4) supplemented with protease and phosphatase inhibitor cocktail (BiMake, B14002, and B15002). All subsequent steps were performed on ice or at 4°C ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15002, SKU# B15002-(1 mL A 1mL B) x 10, (1 mL A 1mL B) x 10, $384.00 Ask -
bioRxiv - Genomics 2019Quote: ... Cells were washed with cold PBS and incubated in 375 µL lysis buffer (10 mM Tris pH 7.5, 10 mM NaCl, 0.2% NP40, 1× protease inhibitors (Bimake), 37.5 U SUPERase.In RNase inhibitor (Invitrogene) ...
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bioRxiv - Cell Biology 2019Quote: ... Cep290 cDNA fragments between exons 34 and 41 were PCR amplified with Universe High-Fidelity Hot Start DNA polymerase (Bimake) and two primers (F-Cep290-e34 ...
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bioRxiv - Cell Biology 2019Quote: ... protease inhibitor cocktail (Bimake)) by pipetting and vortexing ...
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bioRxiv - Bioengineering 2019Quote: ... they were genotyped by PCR using Mouse Direct PCR Kit (Bimake), following the manufacturers instruction ...
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bioRxiv - Physiology 2019Quote: ... Liver samples were homogenized on ice in buffer containing protease cocktail inhibitor (Bimake) and quantified using a fluorometric glycogen assay kit according to manufacturer’s instructions (Cayman Chemicals).
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bioRxiv - Physiology 2019Quote: ... EDTA- and protease cocktail-treated (Bimake) plasma samples were collected at 0 and 30 minutes post-insulin injection for circulating glucagon measurement.
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bioRxiv - Plant Biology 2019Quote: ... 1% (v/v) Protease Inhibitor Cocktail (Bimake, added immediately prior to use) was added to the frozen powder (2 ml per g fresh weight ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were prepared with RIPA lysis buffer and 1X protease inhibitor cocktail (Bimake) and then ground for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... This DNA fragment was inserted into pYES2-SUP35NMG plasmid cut with PvuII and BalI using quick-fusion cloning kit (no longer produced by Bimake, USA) to replace the related sequence ...
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bioRxiv - Microbiology 2019Quote: ... and ligated using a Quick-Fusion cloning kit (Bimake). Component B’ was then generated by PstI digestion to remove the part downstream of bisD and religated ...
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bioRxiv - Immunology 2019Quote: ... and qPCRs were performed using SYBR Green qPCR Mastermix (Bimake) on a BioRad CFX Connect thermocycler ...
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bioRxiv - Developmental Biology 2019Quote: Mouse tails and AG-haESCs were lysed by Mouse Direct PCR Kit (Bimake) according to the manufacturer’s guidance ...
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bioRxiv - Cell Biology 2019Quote: ... and inserted into PvuII/BalI treated pYES2-Sup35NMH-GFP using quick-fusion cloning kit (Bimake) to replace fragment encoding Sup35 residues 1-154.
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bioRxiv - Cancer Biology 2019Quote: ... and Protease Inhibitor Cocktail (Bimake). The Pierce BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and Complete protease inhibitor cocktail (cat#: B14011, Bimake)] ...
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bioRxiv - Cell Biology 2019Quote: ... additional Phosphatase Inhibitor Cocktail (cat#: B15001, Bimake) will be applied if phosphorylation signal is to be detected ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... pH 7.4) supplemented with protease and phosphatase inhibitor cocktails (BiMake), and centrifuged at 13,000xg for 10 minutes at 4°C.
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bioRxiv - Plant Biology 2019Quote: ... Three-step qPCR of cDNA was carried out using SYBR-Green Master Mix (Bimake). qPCR reactions were run in triplicate and an average taken ...
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bioRxiv - Molecular Biology 2019Quote: The total protein was extracted in SCC9-FAP and IP was performed using a Protein A/G Magnetic Beads (Bimake, USA). Briefly ...