Labshake search
Citations for Bimake :
1 - 50 of 258 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... 0.1% (w/v) SDS supplemented with protease and phosphatase inhibitor cocktail (BiMake)) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cDNAs were analyzed by real time PCR using SYBR Green (Bimake, 21203) DNA quantification kit ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% Triton X-100) supplemented with Protease Inhibitor Cocktail (Bimake). Lysates were centrifuged at 20,000x g for 15 minutes at 4°C to precipitate insoluble materials.
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclei were then washed twice with cold PBS and resuspended in SDS lysis buffer (50 mM Tris– HCl, pH8.1, 10 mM EDTA, 0.5% SDS, 1×Protease inhibitor cocktail (Bimake)) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Viability was measured using the cell counting kit-8 (CCK-8, Bimake) and read on a Biotek Synergy 2 plate reader ...
-
bioRxiv - Neuroscience 2024Quote: ... qPCR analysis was carried out using SYBR-Green Premix Ex Taq (Bimake, Cat. B21203, China) on a ViiA7 Real-time PCR system (Applied Biosystems ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21203, SKU# B21203-25 mL, 25 mL, $580.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... and Phosphatase inhibitor cocktail (Bimake Cat# B15001). Cell lysates were incubated with precipitating antibody against Dab1 (mouse anti-Dab1 #2721 ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... 1% EDTA-free protease inhibitor cocktail (B14001, Bimake) and 1% phosphatase inhibitor cocktail (B15001 ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Biochemistry 2024Quote: ... remove the old medium and add 100 μL fresh medium with 10% CCK8 reagent (Bimake; Selleck Chemicals; cat. no. B34304) for each well ...
Product cited:
The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell...Cat# B34304, SKU# B34304-25 mL (2500 rxns), 25 mL (2500 rxns), $201.00 Ask -
bioRxiv - Molecular Biology 2024Quote: ... 1% w/v deoxycholic acid (sodium salt)) supplemented with protease inhibitors (Bimake, Cat# B14001) followed by sonication ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14001, SKU# B14001-1 mL, 1 mL, $37.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... and 1% phosphatase inhibitor cocktail (B15001, Bimake) at 4°C for 20 minutes ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Molecular Biology 2024Quote: ... SYBR Green qPCR Master Mix (High ROX) (Bimake) and custom designed oligos were used to quantify mRNAs ...
-
bioRxiv - Cell Biology 2024Quote: ... and nuclei were prepared by incubation in FL buffer (5 mM PIPES, pH 8.0, 85 mM KCl, 0.5% NP40) supplemented with Protease Inhibitor Cocktail (Bimake) and Phosphatase Inhibitor Cocktail (Bimake ...
-
bioRxiv - Cell Biology 2024Quote: ... and Phosphatase Inhibitor Cocktail (Bimake) for 30 min on ice ...
-
bioRxiv - Biochemistry 2024Quote: Cells were lysed in RIPA buffer (Beyotime, Haimen, China) supplemented with phosphatase inhibitors (Bimake; Selleck Chemicals, Houston, TX, USA) or protease inhibitor cocktail (Roche ...
-
bioRxiv - Cancer Biology 2024Quote: ... anti-flag magnetic beads (Bimake) were added to the cell lysate ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5 mM EDTA and 1x protease inhibitor (Bimake, cat. B14002). Protein concentration was measured by using the Bradford assay (BioVision ...
-
bioRxiv - Biophysics 2024Quote: ... complete mini EDTA-free protease inhibitor (Bimake, B14001), and 1 U/μL of RNasin Plus RNase Inhibitor (Vazyme ...
-
bioRxiv - Genetics 2024Quote: ... The cell lysate was then incubated with prewashed Magnetic Beads (Bimake, Texas, USA) and primary antibodies at 4°C overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... The genotypes of mice were identified by PCR analysis of tail DNA according to the manufacturer’s instructions (Mouse Direct PCR Kit, Bimake, B40013). Heterozygous double-transgenic mice aged 4-6 months were used for lineage tracing ...
-
bioRxiv - Cell Biology 2024Quote: cDNA was subjected to RT-qPCR using the SYBR Green assay with 2× SYBR Green qPCR master mix (Bimake). PCR reactions were performed on a CFX Connect Real-Time PCR Detection System (Bio-rad ...
-
bioRxiv - Neuroscience 2024Quote: Dentate gyrus tissues were carefully dissected, lysed in RIPA buffer (P0013B, Beyotime Biotechnology) with 1× Protease inhibitor cocktail (B14001, Bimake), sonicated (Diagenode ...
-
bioRxiv - Developmental Biology 2024Quote: ... 10% protease inhibitor cocktail (Bimake, B14012) and a lab-made phosphatase inhibitors cocktail (8 mM NaF ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 mM DTT) containing Protease Inhibitors EDTA-free (Bimake) and Benzonase (Merck) ...
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA was used as the template for the quantitative PCR assay using 2×SYBR Green qPCR Master Mix (Bimake, B21202). Quantitative PCR primers are listed in Table S8.
-
bioRxiv - Molecular Biology 2024Quote: ... which was obtained by collecting extra-embryonic regions subsequently digestion using Mouse Direct PCR Kit (Bimake, B40013). PCR reactions were used to detect the Cre transgene and the Hand1 loxP site ...
-
bioRxiv - Developmental Biology 2024Quote: ... All manipulations were carried out on ice and all buffers contain a mix of protease inhibitor cocktail (Bimake B14012).
-
bioRxiv - Immunology 2024Quote: ... 2xSYBR Green qPCR Master Mix (Low ROX) (Bimake) was used to perform qPCR ...
-
bioRxiv - Immunology 2024Quote: ... qPCR reactions included a 2x SYBR Green qPCR Master Mix (Low ROX) (Bimake), 300 nM of each specific primer and 2 μL of diluted cDNA as template ...
-
bioRxiv - Neuroscience 2024Quote: Hippocampal dentate gyrus was rapidly stripped and sonicated (BioruptorTM UCD-200) in RIPA buffer (P0013B, Beyotime Biotechnology) with 1% cocktail (B14001, Bimake) followed by centrifuge (12000rpm ...
-
bioRxiv - Neuroscience 2024Quote: ... and protease inhibitor cocktail (B14002, Bimake)] ...
-
bioRxiv - Molecular Biology 2024Quote: ... 229.73 ng/ml creatine kinase and 1x protease inhibitor cocktail (Bimake, Cat. No. B14002)) to a final concentration of 2×108 cells/ml in 2 ml screw cap microtubes (Sarstedt ...
-
bioRxiv - Molecular Biology 2024Quote: Tissues were homogenized in RIPA buffer (EMD Millipore with protease and phosphatase inhibitor cocktail (Bimake.com, #B14002, #B15002). Proteins were separated using SDS-PAGE and transferred to the Nitrocellulose membrane (BioRad ...
-
bioRxiv - Neuroscience 2024Quote: ... and 1x phosphatase inhibitor (catalog no. B15002; Bimake). For homogenization ...
-
bioRxiv - Neuroscience 2024Quote: ... and ice-cold Homogenization Buffer (Tris-EDTA buffer, pH 8.0 [ThermoFisher Scientific AM9858] and 1X Protease [Millipore 539131] and Phosphatase Inhibitor Cocktail [Bimake B15002]) was added ...
-
bioRxiv - Molecular Biology 2024Quote: ... DNA Transfection Reagent B35101 (Bimake), PEI MW40000 ...
-
bioRxiv - Immunology 2024Quote: ... supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 × protease inhibitor cocktail (Bimake). The suspension was rotated for 3 hours at 4℃ followed by centrifugation at 8,000 rpm for 40 minutes and ultracentrifugation at 40,000 rpm for 1 hour ...
-
bioRxiv - Neuroscience 2024Quote: ... with 0.5 mm zirconium oxide beads and ice-cold Triton X-100 buffer containing 1x protease inhibitor (catalog no. B14002; Bimake) and 1x phosphatase inhibitor (catalog no ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1% NP-40, and 0.25% sodium deoxycholate) supplemented with phenylmethylsulfonyl fluotide (PMSF; ST505, Beyotime Biotechnology) and phosphatase inhibitor cocktail (B15001, Bimake, USA). To immunoprecipitate PRMT5 ...
-
bioRxiv - Cancer Biology 2024Quote: ... phosphatase inhibitor cocktail (B15001, Bimake), and protease inhibitors (539134 ...
-
bioRxiv - Cancer Biology 2024Quote: ... or 2 × SYBR Green qPCR Master Mix (Low ROX, B21702, Bimake). All quantities were normalized to those of endogenous ACTB (β-actin) ...
-
bioRxiv - Biochemistry 2024Quote: ... 1x protease inhibitor cocktail (Bimake, Cat No. B14002)) utilized in this work was not supplemented with amino acids.
-
bioRxiv - Biochemistry 2024Quote: ... 1x protease inhibitor cocktail (Bimake, Cat No. B14002), 15% glycerol (unless otherwise stated) ...
-
bioRxiv - Cancer Biology 2024Quote: Cell viability was evaluated using the Cell Counting Kit-8 (CCK-8) assay (B34304, Bimake). For this ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1% NP-40, and 0.25% sodium deoxycholate) supplemented with PMSF (ST505, Beyotime Biotechnology) and phosphatase inhibitor cocktail (B15001, Bimake). To immunoprecipitate Flag-PRMT5 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the SYBR Green PCR Core Reagents kit (Bimake.com). The thermal cycling conditions comprised an initial denaturation step at 94 °C for 5 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... and protease inhibitor cocktail (Bimake).
-
bioRxiv - Cell Biology 2024Quote: ... and lysed in lysis buffer (20 mM Tris pH=8.0, 150 mM NaCl, 1% Triton) containing protease inhibitor cocktail (Bimake) at 4°C for 20 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... qRT-PCR reactions were performed with SYBR Green supermix (Bimake, B21203) using the ABI 7500 Fast platform (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 μl of anti-Flag magnetic beads (Bimake, B26101) were added and the mixture was incubated on a rotator at 4°C overnight ...