Labshake search
Citations for Bimake :
101 - 150 of 258 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... 1 mM MnCl2 and 100 μM TCEP supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization ...
-
bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR was performed by using the Agilent Mx3000P qPCR system with the 2×SYBR Green qPCR Master Mix (Bimake). Relative mRNA expression was determined by the delta cycle time with human GAPDH as the internal control in data normalization ...
-
bioRxiv - Cell Biology 2022Quote: ... primers and SYBR-green fluorescent dye (Bimake, Houston, TX, USA) were used ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells or fresh tissues were lysed on ice using RIPA buffer supplemented with protease inhibitors (Bimake, B14012) and phosphatase inhibitors (Roche ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1mM MgCl2 and 12U/ml Benzonase) supplemented with protease inhibitors (Bimake). Antibodies (FLAG ...
-
bioRxiv - Developmental Biology 2022Quote: ... and protease inhibitors (Bimake).
-
bioRxiv - Cell Biology 2022Quote: ... 2 μL of 1:10 diluted cDNA was added to 10 μL of 2x SYBR Green qPCR Master Mix (Low Rox) (Bimake, Houston, TX, USA). The final concentration of each primer was 250 nM in 20 μL of total master mix volume ...
-
bioRxiv - Cancer Biology 2022Quote: ... and protease inhibitor cocktail (Bimake). The Pierce bicinchoninic acid (BCA ...
-
bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed using 2X SYBR green master mix (Bimake). Relative gene expression was determined using ddCt method with Ribosomal protein L9 (rpl9 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1% NP-40 and 5% glycerol) supplemented with protease inhibitors (Bimake, Cat# B14012) and phosphatase inhibitors (Roche ...
-
bioRxiv - Cell Biology 2022Quote: ... 2X SYBR Green PCR Master Mix was purchased from Bimake. Real-time quantitative PCR was conducted using the QuantStudionTM 7 flex System (Applied Biosytstems) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and the SYBR Green PCR Core Reagents kit (Bimake.com). The thermal cycling conditions comprised an initial denaturation step at 94°C for 5 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... and reverse transcribed using the All-in-One cDNA Synthesis Kit (Bimake, B24403). qRT-PCR was performed using SYBR Green qPCR Master Mix (Bimake ...
-
bioRxiv - Cell Biology 2022Quote: ... Protease Inhibitor Cocktail (Bimake, B14012)] and incubated for 30 min at 4°C with rotation ...
-
bioRxiv - Biochemistry 2022Quote: ... supplemented with proteasome inhibitors (Bimake no. B14011). Cell lysates were centrifuged at 15,000 rpm for 15 min ...
-
bioRxiv - Biochemistry 2022Quote: ... Beyotime) supplemented with proteasome inhibitors (Bimake no. B14011). Lysates were centrifuged at 15,000 rpm for 15 min ...
-
bioRxiv - Biochemistry 2022Quote: ... and the elution was done with the and eluted by addition of 0.3 mg/mL poly-FLAG peptide (Bimake, catalog number B23111) to the lysis buffer ...
Product cited:
The Poly DYKDDDDK (FLAG) Peptide lyophilized powder was synthesized by 23 amino acid residues...Cat# B23111, SKU# B23111-4mg, 4mg, $299.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR was performed using SYBR Green qPCR Master Mix (Bimake, B21202). Gapdh was used for normalization ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21202, SKU# B21202-5 mL, 5 mL, $137.00 Ask -
bioRxiv - Microbiology 2022Quote: Cell viability assays were performed using a Cell Counting Kit-8 (CCK-8, Bimake, #B34302) according to the manufacturer’s instructions ...
Product cited:
The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell...Cat# B34302, SKU# B34302-5 mL (500 rxns), 5 mL (500 rxns), $71.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using SYBR Green qPCR master mix (Bimake, B21202). The enrichment of the Kit and Sp1 promoter regions was calculated relative to the IgG control ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21202, SKU# B21202-5 mL, 5 mL, $137.00 Ask -
bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were incubated with anti-Flag magnetic beads (Bimake, catalog number B26102) for 2 h at 4 °C ...
Product cited:
Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1...Cat# B26102, SKU# B26102-5 mL, 5 mL, $999.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... 5 mM EDTA) containing protease/phosphatase inhibitor (B14001/B15001, Bimake). After centrifugation at 14,000 × g (4°C ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Immunology 2022Quote: ... and each sample was analyzed in triplicate using 2X SYBR Green PCR mix (Cat#: B21202, Bimake.com) (Supplemental Table 6) ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21202, SKU# B21202-5 mL, 5 mL, $137.00 Ask -
bioRxiv - Microbiology 2022Quote: ... Virus quantification by real-time PCR was performed using the QPCR Mix (Bimake, Cat#B21202), following the supplier’s instruction ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21202, SKU# B21202-5 mL, 5 mL, $137.00 Ask -
bioRxiv - Biochemistry 2022Quote: ... The qPCR was performed with 2x SYBR Green qPCR Master Mix (Bimake B21202) on the Biorad CFX Connect Real-Time PCR detection system ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21202, SKU# B21202-5 mL, 5 mL, $137.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... supplemented with protease inhibitor cocktail (Bimake, B14002), phosphatase inhibitor cocktail III (Research Products International ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14002, SKU# B14002-1 mL x 10, 1 mL x 10, $270.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... The lysate was incubated with 25 μL of anti-Flag magnetic beads (Bimake, B26101) for 12 hrs at 4°C on a rotator ...
Product cited:
Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1...Cat# B26101, SKU# B26101-1 mL, 1 mL, $319.00 Ask -
bioRxiv - Microbiology 2022Quote: ... The eluates were further purified with magnetic beads conjugated with mouse anti-FLAG antibody (Bimake, Shanghai, China) (15 μL/dish) ...
-
bioRxiv - Immunology 2022Quote: ... phosphatase inhibitor cocktail (Bimake), and SDS loading buffer (Beyotime Biotechnology) ...
-
Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... 1x protease inhibitor cocktail (Bimake, Houston, USA), and centrifuged at 12,000 g for 10min to remove nuclei ...
-
bioRxiv - Neuroscience 2022Quote: ... Control EC and CD2AP EC-KO murine brain samples were chopped and frozen in 0.5 mL of MIB containing 0.32 M sucrose and protease and phophatase inhibitors (Bimake) until processed for microvessel enrichment ...
-
bioRxiv - Neuroscience 2022Quote: ... 3 mM CaCl2 and 12 mM MgCl2) containing protease and phosphatase inhibitor cocktails (Bimake, Houston, TX), meninges and white matter were removed ...
-
bioRxiv - Biochemistry 2022Quote: ... protease inhibitor cocktail (Bimake, 1 mL/ 100 mL suspension). The protein complex was assembled on membrane by adding 100 μM rotigotine (TargetMol ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 μl of Power SYBR Green PCR Master Mix (Bimake), 0.4 μl of reference ROX dye and 7.6 μl of milliQ sterile water were added to 1 μl cDNA together with 1 μl of 10 μM forward and reverse primer mix (mTMX4 (I ...
-
bioRxiv - Biochemistry 2022Quote: ... 20 mM KCl and 10 mM MgCl2 supplemented with Protease Inhibitor Cocktail (Bimake). The sample was centrifuged at 65,000 g for 30 min ...
-
bioRxiv - Cancer Biology 2022Quote: Mice were genotyped by using the Mouse Direct PCR kit (Bimake) on DNA isolated from tails following the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed in triplicate or quadruplicate using 2 x SYBR Green qPCR Master Mix (Bimake.com) with specific primers and normalized to reference genes (Table 1).
-
bioRxiv - Developmental Biology 2022Quote: ... combined with the protease inhibitor cocktail (Bimake, Shanghai, China) for Western blot ...
-
bioRxiv - Developmental Biology 2022Quote: Female adult ovaries of Drosophila were collected with gathered into lysis buffer RIPA (Meilunbio, Dalian, China) with protease inhibitor cocktail (Bimake, Shanghai, China) for Western blotting ...
-
bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed with SYBR Green (Bimake). All qPCR gene expression data were normalized against the housekeeping gene ACTB.
-
bioRxiv - Genomics 2022Quote: ... using wAlbA-specific primers (Table S5) normalised to the host homothorax (HTH) gene using a SYBR Green qPCR master mix (Bimake), with the following conditions ...
-
bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% (v/v) glycerol and EDTA-free protease inhibitor mixture (Bimake)] ...
-
bioRxiv - Pathology 2022Quote: ... and RT-qPCR was performed using SYBR Green Super-Mix (Bimake, China) on an RT-qPCR machine (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2022Quote: ... and phosphatase inhibitor cocktail (100X) (Bimake). The lysates were cleared by centrifugation at 13,000 rpm at 4°C in a microcentrifuge for 10 minutes ...
-
bioRxiv - Biochemistry 2022Quote: Cells were lysed in RIPA buffer (Thermo) containing protease and phosphatase inhibitor cocktails (Bimake B14001 and B15001-B). Protein concentration was quantified by BCA assay (Thermo Fisher) ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Microbiology 2022Quote: ... VmlR2viretiEQ2-HTF was eluted by addition of 200 µL opening buffer containing 0.1 mg/mL poly-FLAG peptide (Biotool, Bimake) for 45 min on a turning wheel ...
-
bioRxiv - Cell Biology 2022Quote: ... 1X Protease Inhibitor Cocktail (B14002, Bimake), Phosphatase Inhibitor Cocktail (B15001 ...
Product cited:
Protease Inhibitor Cocktail comprehensively protects proteins from degradation by a variety of...Cat# B14002, SKU# B14002-1 mL x 10, 1 mL x 10, $270.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... Phosphatase Inhibitor Cocktail (B15001, Bimake). Lysates were then cleared by centrifugation and the beads were blocked with 1% BSA to avoid non-specific binding of PRMT5 to Glutathione beads ...
Product cited:
Phosphatase Inhibitor Cocktail for phosphorylation protection is provided in a 2 tube formula at...Cat# B15001, SKU# B15001-1 mL A + 1mL B, 1 mL A + 1mL B, $54.00 Ask -
bioRxiv - Physiology 2022Quote: ... and SYBR Green qPCR Master Mix (B21203, BiMake) in a 96-well plate ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21203, SKU# B21203-25 mL, 25 mL, $580.00 Ask -
bioRxiv - Immunology 2022Quote: ... 95048-100) was used to produce cDNA before running RT-qPCR with 2x SYBR Green qPCR Master Mix (BiMake, B21203) according to manufacturer instructions ...
Product cited:
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...Cat# B21203, SKU# B21203-25 mL, 25 mL, $580.00 Ask