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Citations for Bio-Rad :
251 - 300 of 3384 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 0.5 µg of each RNA sample was used in a 10-µL reverse transcription reaction set up using the iScript Select cDNA synthesis kit (Bio-Rad), together with gene-specific reverse primers (Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples were cleaned up and used as template for quantitative PCR (qPCR) using iTaq Universal SYBR Green Supermix (Bio-Rad) and primers specific for the genes indicated (primers are listed in Table 2 ...
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bioRxiv - Microbiology 2020Quote: DNA samples were cleaned up and used as template for quantitative PCR (qPCR) using iTaq Universal SYBR Green Supermix (Bio-Rad) and primers specific for the genes indicated (primers are listed in Table S3 ...
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bioRxiv - Cancer Biology 2021Quote: ... qPCR reactions were set up in triplicate in a 384-well plate and run on the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). Each reaction contained 5μL of PerfeCTa SYBR Green FastMix (Quantabio ...
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bioRxiv - Microbiology 2020Quote: ... 12-16 replicate wells of up to 800 ng DNA each were combined with 2X ddPCR Supermix for Probes (no dUTP, Bio-Rad) and primer/probe sets for regions in PS and env that favors amplification of intact proviral genomes ...
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bioRxiv - Molecular Biology 2022Quote: ... Membrane was placed face up on dry Whatman filter paper and crosslinked with 125 mJoule in a GS Gene Linker UV Chamber (Bio-Rad) using the C-L setting ...
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bioRxiv - Molecular Biology 2023Quote: ... The fragmentation step was then stopped by adding Tris pH 6.8 (0.4 M final concentration) and then samples were cleaned-up on Micro Bio-Spin P-30 gel columns (Bio-Rad Laboratories). 4sU-RNA was then biotinylated using 0.1 mg/ml MTSEA biotin-XX linker (Biotium ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Genomics 2023Quote: ... and 500 nM each primer up to a total volume of 10 μl and amplified in a CFX96 Real-Time System (Bio-Rad) with the following program ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was generated by reverse transcription of up to 9 ng RNA from each sample using iScript Reverse Transcription Supermix for RT-qPCR (BioRad, 1708841). mRNA expression was determined on a StepOnePlusTM Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... For Western blotting equal amounts (up to 80 μg) of total cell lysates were loaded on SDS–polyacrylamide gel electrophoresis system (Bio-Rad) and transferred on nitrocellulose membrane which was proceeded for immunoblotting to detect specific proteins ...
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bioRxiv - Biophysics 2024Quote: ... The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated in a 5 ml 1:1 A:B solution of Clarity Western ECL substrate (BioRad cat. #170-5060) for 5 minutes at room temperature with rotation ...
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bioRxiv - Plant Biology 2021Quote: ... and 1 ml Luminol/enhancer solution (Clarity™ Western ECL, BIORAD, Les-Ulis, France) and observed using ChemiDoc (ChemiDoc™ Imaging Systems ...
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bioRxiv - Cell Biology 2023Quote: Polyacrylamide gels of 7.9 kPa stiffness were made by adjusting acrylamide and bisacrylamide stock solution (Bio-Rad Laboratories, Hercules, CA) concentrations ...
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bioRxiv - Microbiology 2021Quote: ... The suspensions were then distributed into 0.2 mL tubes at approximately 1 × 108 CFU/ml and incubated in a C1000 Thermal Cycler (Bio-Rad Laboratories, Foster City, CA) at 50
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bioRxiv - Microbiology 2020Quote: ... Up to 8 replicate wells of up to 500 ng of DNA was added to ddPCR™ Supermix for Probes (No dUTP) (Bio-Rad) with a primer/probe set overlapping the LTR-gag junction designed to measure group-M HIV-1 proviral load (10) ...
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bioRxiv - Molecular Biology 2023Quote: ... heated up for 10 minutes at 95°C and centrifuged before loading onto a 10 well 5-20% gradient gel (BioRad 456-1093). The finished gels were blotted via semi dry method (20V ...
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bioRxiv - Developmental Biology 2022Quote: ... The suspended eggs and siRNA (5 μg/μl stock solution, in MilliQ) were transferred to a 4 mm cuvette (Bio-Rad) with a final volume of 200 μl and were gently mixed ...
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bioRxiv - Molecular Biology 2019Quote: ... at a 1 ml flow rate using a NGC Quest 10 chromatography system (Bio-Rad). Buffer A was 5% acetonitrile 0.01M ammonium bicarbonate ...
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bioRxiv - Microbiology 2022Quote: ... Detergents were removed by incubation with 0.8 mg ml-1 Bio-Beads SM2 (Bio-Rad) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Detergents were removed by incubation with 0.8 mg ml-1 Bio-Beads SM2 (Bio-Rad) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with PBST containing 1% BSA and 22.5 mg/mL glycine (Bio-Rad 1610718) for 30 minutes at 23°C ...
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bioRxiv - Microbiology 2023Quote: Overnight cultures in TSB were diluted 100-fold into 1 mL of Chelex (Bio-Rad)- treated TSB with the addition of 25 μM zinc acetate ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred into clean tubes and the protein concentration was determined using the Bradford protein assay kit (Bio-Rad Laboratories, USA). The protein extracts (20 μg ...
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bioRxiv - Neuroscience 2022Quote: ... The clear supernatants were then transferred into clean tubes and total protein concentration measured using a DC protein assay (Bio-Rad, Hercules, CA). To prepare for ELISA ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was washed 3 times with TBST for 5 minutes and was incubated with 10 mL of 3% BSA/TBST (w/v) with 1 µL anti-mouse-HRP secondary antibody (1:10,000; Bio-Rad, 170-6516) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Partitioning of the reaction mixture into up to 20,000 individual droplets was achieved using a QX200 dPCR droplet generator (Bio-Rad Laboratories, Munich, Germany). A two-step PCR-reaction was performed on a Mastercycler Pro instrument (Eppendorf ...
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bioRxiv - Genomics 2021Quote: ... Partitioning of the reaction mixture into up to 20,000 droplets was carried out on a QX200 ddPCR droplet generator (Bio-Rad, Laboratories, Munich, Germany) and PCR was performed using a Mastercycler Pro (Eppendorf ...
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bioRxiv - Molecular Biology 2021Quote: PCR reactions were set up using the following volumes: 10 µL 2x ddPCR Supermix for probes (no dUTP) (BioRad Laboratories© 186-3024), 5 µL of 20plex oligo mix ...
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bioRxiv - Bioengineering 2020Quote: ... in a run buffer (Supplementary Table S2) consisting of 1X tris-glycine (prepared by ultrapure water dilution of 10X stock, Bio-Rad 1610734) containing 0.5% v/v Triton X-100 (Sigma) ...
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bioRxiv - Genetics 2020Quote: ... in 11 parallel transformations (1 mL each) on a Bio-Rad MicroPulser (Bio-Rad, #165-2100). The parallel transformations were combined and mixed with a total of 9.5 mL of recovery medium ...
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bioRxiv - Biochemistry 2021Quote: ... the detergent was removed by adding 0.5 g mL-1 (w/v) Bio-Beads (Bio-Rad) overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... Nanodisc reconstitution was achieved by incubation with 0.5 - 1 mL Bio-Beads SM-2 (Bio-Rad) for 16 hours at 4°C under constant rotation ...
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bioRxiv - Biochemistry 2021Quote: ... surface CD8 was labeled with 10 µg ml−1 AlexaFluor647-anti-CD8 antibody (Bio-Rad, MCA1226A647) at 37 °C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... Up to 1000 ng of RNA was used to synthesise cDNA using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA, #1725035) following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: The different gel rigidities were achieved by mixing different ratios of gel precursors acrylamide (AA) (stock 40 %, #1610140, Bio-Rad Laboratories, Hercules, USA) and bis-acrylamide (Bis ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: A 1 mL Ni-NTA resin in a glass gravity-flow column (Econo-Column®, BioRad, USA) was set up inside a Coy anaerobic glovebox with an atmosphere of 100% N2 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant contained 2.5 mg proteins/ 1 ml (determined using Bio-Rad Protein Assay, Cat. No. 5000006). This protein fraction was used for co-immunoprecipitation (input) ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 was concentrated to 1-mL and isocratically eluted from an ENrich SEC650 10×300 column (BioRad) equilibrated with Buffer B ...
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bioRxiv - Immunology 2020Quote: ... followed by streptavidin-alkaline phosphatase (1 µg/mL) and development with AP conjugate substrate kit (Bio-Rad). For analysis of intracellular cytokine production ...
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bioRxiv - Cancer Biology 2023Quote: 6-well culture plates were coated with 1 ml of 0.6 % Low Melt Agarose (Bio-Rad; 1613111). Cells were diluted at a density of 1.0 x 104 cells/ml in media containing 0.3% Low Melt Agarose and seeded 1 ml/ well ...
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bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The content of each well was then drawn up into the Bio-Plex 100 System array reader (Bio-Rad Laboratories Bio-Rad Laboratories, Hercules California, USA), which identifies and quantifies each specific reaction based on bead color and fluorescent signal intensity ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Tween 20) at room temperature for 1 hour and stained with 0.3 μg/mL streptavidin-HRP (BioRad) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... HRP detection was performed by adding 1 mL Clarity Max™ Western ECL Blotting Substrates from Bio-Rad. Arrays were imaged using BioRad X-ray imager and quantified using ImageJ ...
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Bioengineering 2019Quote: ... Blots were subsequently incubated with 0.33 µg ml-1 goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 30 min at room temperature with α-CD3 (1 µg/mL; clone UCHT1; Bio-Rad). CD8+ T cells were negatively selected from blood and TDL using a CD8+ T Cell Enrichment Kit (stemCell Technologies) ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies were used: mouse anti-talin1 (clone 97H6, Bio-Rad: MCA4770, Lot#:1803, 1 μg/ml), rabbit anti-zyxin (B71 ...