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Citations for Bio-Rad :
201 - 250 of 3384 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... and RT-qPCR assays were set up with 2.5 μL SsoAdvanced Universal SYBR Green Supermix (BioRad, Hercules, CA), 1.25 pmol of each primer ...
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bioRxiv - Genetics 2022Quote: ... Up to 40μg of total protein was loaded onto a Mini-PROTEAN TGX gel (Bio-Rad, 456-8093) and separated by SDS-PAGE for 1 hour at 200 volts ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions were set up according to the manufacturer’s instructions in a T100™ Thermal Cycler (Bio-Rad). SA11 VP4 PCR conditions were 1 cycle of 98°C for 30 sec ...
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bioRxiv - Genetics 2022Quote: ... and qRT-PCR reactions were set up using SsoAdvanced Universal SYBR Green or SsoFast™ EvaGreen Supermix (BioRad). Reactions were run on the StepOne Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... The slides were covered in a blocking solution made up of in Tris-buffered saline (#1706435; Bio-Rad) with 0.1% (vol/vol ...
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bioRxiv - Physiology 2022Quote: ... and protein content assessed by BCA assay (Pierce) before 15 ug protein lysates were separated on a TGX Stain-Free™ criterion gel (Bio-Rad, Sweden). Following which ...
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bioRxiv - Immunology 2021Quote: ... Basically 300 ug of IVIG protein were subjected to isoelectric focusing (IPG) across pH gradients of 4-7 (Biorad, LifeSciences, Hercules, Ca, USA) or pH 6-11 (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ug of protein was loaded onto precast gel (4561095, 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Bio-Rad, USA) and resolved at 200 volts for 30-35 minutes ...
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bioRxiv - Genomics 2023Quote: ... 2 million cells were resuspended in 200μl OptiMEM and incubated for 3min with 2μg (7.5μL of a 20μM stock) siRNA in a 4mm cuvette (Bio-Rad) before being pulsed at 300V ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Biochemistry 2022Quote: Supernatants from Wuhan SARS CoV-2 infected cells prepared as described above or uninfected cells and commercial spike were lysed with 2x Laemmli Sample Buffer (#1610737, Bio-Rad, Hercules, CA, USA) with DTT (#EU0006-B ...
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bioRxiv - Microbiology 2023Quote: ... Products were purified using the ssDNA clean and concentrate kit and loaded on 10% TBE-Urea gels (BioRad #4566033) with sample buffer (Thomas Scientific #C995V39 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-IBA-1 antibody (0.4 μg/mL, FUJIFILM Wako) and rat anti-CD68 antibody (5 μg/mL, clone FA-11, Bio-Rad) was used ...
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bioRxiv - Biophysics 2021Quote: ... Purified TREK-2 was then mixed with GUVs to a final concentration of ∼1 - 5 µg/ml and incubated overnight at 4 °C with 0.5mg/ml Bio-Beads (Bio-Rad) prior to use.
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bioRxiv - Biophysics 2020Quote: ... 1 mL of 10X tris-borate-EDTA (TBE) buffer (Bio-Rad Laboratories), 20 μL of freshly made ammonium persulfate (APS ...
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bioRxiv - Immunology 2020Quote: ... The beads were transferred to a 1 mL spin columns (Bio-Rad) and equilibrated in 100 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... were coated with 1 μg/ml recombinant YFV NS1 (Biorad, cat # PIP052A) in 50 mM carbonate buffer (pH ...
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bioRxiv - Biochemistry 2021Quote: Purified proteins concentrated to 1 mL or gel filtration standards (BioRad, 1511901) were injected on to Hi-Load pg200 column and 150 mL eluate was collected as 1.0 mL fractions at 1.0 mL/min. ...
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bioRxiv - Immunology 2022Quote: ... followed by installation of 0.5 ml of 1% low melting agarose (BioRad) via the trachea ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR reactions were set up using the iTaq Universal Sybr Green One-Step RT-PCR kit (Biorad) according to manufacturer’s directions ...
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bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
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bioRxiv - Molecular Biology 2023Quote: ... The content of each well was then drawn up into the Bio-Plex 100 System array reader (Bio-Rad Laboratories Bio-Rad Laboratories ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by incubation at 37 °C for up to 7 hours on a real-time qPCR instrument (Biorad, CFX96). To measure the time-dependent change in fluorescence ...
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bioRxiv - Evolutionary Biology 2024Quote: ... This reaction mixture was partitioned into up to 20,000 nanoliter-sized droplets using the QX200 droplet generator (Bio-Rad) by mixing 70 μl of synthetic oil with 20 μl PCR mix ...
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bioRxiv - Plant Biology 2021Quote: ... membrane was incubated 1 min in 1 ml enzyme solution (Clarity™ Western ECL, BIORAD, Les-Ulis, France) and 1 ml Luminol/enhancer solution (Clarity™ Western ECL ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
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bioRxiv - Molecular Biology 2019Quote: ... EtOH precipitation was performed and DNA was additionally cleaned up on Micro Bio-Spin 6 chromatography columns in SSC (BioRad) as purity was essential for oxidation of hmC ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA template (up to 8 μL) and nuclease-free water were loaded into a disposable droplet generator cartridge (Bio-Rad). After adding 70 μL of droplet generation oil (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... Up to 500 ng of RNA was used for reverse transcription using either the iScriptTM cDNA synthesis kit (Bio-Rad) or the qScript cDNA Synthesis Kit (Quantabio) ...
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bioRxiv - Systems Biology 2022Quote: ... Protein extracts derived from OBC1 cells were electrophoretically transferred onto nitrocellulose membranes using a Trans-blot Turbo transfer system (up to 25V, 7min) (BioRad). Membranes were probed with primary antibodies at 1:1000 dilution in 5% nonfat milk or BSA according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction for ddPCR Copy Number Variation Assay was set up in 20 μl sample volume containing 10 μl of 2X ddPCR Supermix (no dUTP, BioRad), 1 μl of FAM-labeled target primers/probe (BioRad) ...
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bioRxiv - Genomics 2023Quote: ... Each ddPCR reaction was set up by adding the following: 10 uL of 2X ddPCR Supermix (no dUTP; BioRad #1863023), 2 uL of 10 uM ITR_Forward Primer ...
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bioRxiv - Physiology 2024Quote: ... Real-Time PCR was set up in a 384-well plate and run in a CFX384 Real-Time PCR System (BioRad) using the following protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of up to 1μg of total RNA was performed using the iScript cDNA Synthesis Kit (Bio-Rad, #1725038).
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bioRxiv - Bioengineering 2019Quote: ... 2.99 ml 30 % Acrylamide/Bis 29:1 (Bio-Rad, Cat. No. 161-0156), 112.5 μL 10 % (w/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A solution of rabbit αFITC antibody (1 μg/mL, Bio-Rad 4510-7804) in 2% Milk-PBS was added to each well (100 μL/well ...
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bioRxiv - Biochemistry 2021Quote: ... 200 μl of the resin was loaded onto 1 ml column (Bio-Rad) and washed 3 times with 1 ml of 100 mM TBS buffer pH 8.5 with 0.5 M NaCl and 5 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... An activation cocktail consisting of anti-CD3 (1 μg/mL; Bio-Rad #MCA463EL), recombinant IL-2 (100 U/mL ...
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bioRxiv - Biochemistry 2022Quote: ... 60 mg ml-1 of methanol-activated Bio-Beads SM2 resin (Bio-Rad) was added and allowed to mix overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... sheep polyclonal anti-GFP (Bio-Rad, 4745-1051, 1:1000, 5 µg/mL), and rabbit polyclonal anti-GFP (Novus Biologicals ...
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bioRxiv - Cancer Biology 2021Quote: ... 60 of High Capacity NeutrAvidin slurry (Thermo PI29204) were washed three times with 1 mL 2X RIPA/1mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231) ...
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bioRxiv - Microbiology 2021Quote: Viral stocks or supernatant from DENV growth curves at 120hpi were diluted with 4x Laemmli Sample Buffer (Bio-Rad) and boiled at 95°C for 5 minutes ...
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bioRxiv - Biophysics 2021Quote: ... Urea solutions were purified by passage of the stock solution through an AG501-X8(D) ion-exchange resin (Biorad) before use74.
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bioRxiv - Cell Biology 2023Quote: Polyacrylamide gels of desired stiffness were made by adjusting acrylamide and bisacrylamide stock solution (Bio-Rad Laboratories, Hercules, CA) concentrations (Chopra et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting ddPCR droplets were transferred to a clean 96-well plate and the plate was sealed with a foil lid using a thermal plate sealer from Bio-Rad Laboratories prior to PCR amplification using a Bio-Rad C1000 touch thermal cycler with deep wells ...
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bioRxiv - Cell Biology 2023Quote: ... cleared supernatant was transferred to the new clean Eppendorf tubes and total protein concentration was measured using Bradford assay by Bio-Rad Protein Assay kit (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was transferred to a clean new tube and protein concentration was quantified with the DC Protein Assay kit (Bio-Rad). SDS-PAGE and western blot analysis were conducted as described (Tseng et al. ...
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bioRxiv - Cell Biology 2020Quote: ... For Western Blotting protein samples were normalized up to 25µL and combined with a mixture of 4x Laemmli Sample Buffer (#161-0747, Bio-rad Laboratories) with 450mM DTT (D0632 ...
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bioRxiv - Microbiology 2021Quote: ... Partitioning of the reaction mixture into up to 20,000 droplets was carried out using a QX200 dPCR droplet generator (Bio-Rad, Laboratories) and PCR was performed on the Mastercycler Pro (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... DNA samples were cleaned up and used as template for quantitative PCR (qPCR) using iTaq Universal SYBR Green Supermix (Bio-Rad) and primers specific for the genes indicated (see Table S4 for primers ...