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Citations for Bio-Rad :
301 - 350 of 459 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Protein extracts were resolved by SDS-PAGE on Mini-PROTEAN® TGX Stain-Free™ precast gels (BioRad). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Systems Biology 2021Quote: ... Denatured cellular extracts equivalent to 1/120 OD600 were loaded onto a 7.5% precast Mini-Protean TGX (BioRad) and resolved by SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... growth media was removed from cells and cell extracts prepared by lysis in SDS sample buffer (Bio-Rad) supplemented with β-mercaptoethanol (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extract (4 ug per lane) was separated on a 10% TGX Stain-Free polyacrylamide gel (Bio-Rad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2022Quote: ... Prepared protein extracts were resolved on a 4-20% gradient polyacrylamide gel (Bio-Rad, Cat. No. 456-1093), transferred to Immobilon®-P membrane (Millipore ...
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bioRxiv - Systems Biology 2022Quote: Cell extracts (15 ug) were resolved by SDS-PAGE using 4-15% TGX precast gels (Bio-Rad #4561086) with tris/glycine/SDS buffer and transferred onto PVDF membranes using the Bio-Rad Mini-PROTEAN Electrophoresis System ...
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bioRxiv - Microbiology 2019Quote: ... The protein extracts were separated by SDS-PAGE on a 4-20% Mini Protean TGX gel (Bio-Rad) and then transferred to a PVDF membrane ...
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bioRxiv - Neuroscience 2020Quote: Protein extracts were fractionated by 10% SDS-PAGE and electroblotted onto Immobilion-P polyvinyl difluoride membranes (Bio-rad) in CAPS-based transfer buffer (10 mM CAPS pH 11 ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... Protein extracts were separated by denaturing sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad Laboratories, Hercules, California, US) under reducing conditions and transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Microbiology 2019Quote: ... The resulting cell extract (INPUT) was used to determine the total protein concentration using Bradford assay (Biorad, USA). 10 mg of total proteins were mixed with Magnetic anti-FLAG beads (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Cell extracts were separated by SDS-PAGE on 7.5% polyacrylamide gel and transferred to nitrocellulose membrane (Bio-Rad) for immunodetection ...
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bioRxiv - Molecular Biology 2020Quote: Nuclease-treated PEARL extracts were separated on 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (Bio-Rad) for western blot analysis ...
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bioRxiv - Genetics 2020Quote: Whole cell extracts (WCE) prepared using NaOH lysis method were separated on a 4-20% polyacrylamide gel (BioRad), and transferred using a Semi-Dry Trans-Blot Cell (BioRad) ...
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bioRxiv - Plant Biology 2022Quote: ... and the amount of protein for each extract was measured according to the manufacturer’s instructions (Bio-Rad, USA). Proteins were detected using anti-MBP (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20 µg/µl of the extracts were run on a 10% TGX Stain-free FastCast gel (BioRad) and transferred onto a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 µg of SN or CB protein extracts were blotted onto a nitrocellulose membrane gentle vacuum (Bio-rad), and air dried for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein extracts were loaded and run on 5-20% gradient pre-cast gels (Bio-Rad) before being transferred onto a 0.45 μM nitrocellulose membrane (Amersham ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant protein extract was collected and protein concentration was determined using DC Protein Assay Kit (Bio-Rad).
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Suppression of the Integrated Stress Response in Islet β Cells Decreases Risk of Autoimmune DiabetesbioRxiv - Cell Biology 2023Quote: ... and protein extract was resolved by electrophoresis on a precast 4-20% tris-glycine polyacrylamide gels (Bio-Rad), transferred to polyvinylidene difluoride membrane ...
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bioRxiv - Cell Biology 2023Quote: ... After taking a sample from the cell-free extract (CFE) the chromatography column (BioRad, Poly-Prep Chromatography Column) was prepared by adding 1 ml of resin (His-select nickel affinity gel) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated on 10% or 15% SDS-PAGE gels and transferred to nitrocellulose membrane (Bio-Rad). Membranes were incubated with the primary antibodies listed in Supplemental Table S2 overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein extracts underwent electrophoresis under reducing conditions on 4–15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and then transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: Crude extracts of the given strains were separated on 4-20% Criterion TGX precast midi protein gels (BioRad #5671094). The gel was washed in fixing solution (40% Ethanol 10% Acetic Acid ...
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bioRxiv - Molecular Biology 2021Quote: ... The crude extracts were separate by SDS PAGE on 4-20% Criterion TGX precast midi protein gels (BioRad #5671094) and transferred to a 0.2 μm nitrocellulose membrane (BioRad #1620112 ...
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bioRxiv - Developmental Biology 2020Quote: ... These cell extracts were run on a 10% acrylamide gel before being transferred to a PVDF membrane (Bio-Rad). The membranes were blocked in 5% milk prior to incubation with primary antibodies overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 30 ug of each extract was resolved through 4-20% Criterion Tris-Glycine (TGX) Stain-Free SDS-PAGE (Biorad) and transferred to nitrocellulose ...
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bioRxiv - Molecular Biology 2021Quote: ... The same amounts of protein extracts were separated on the 4%-20% Mini-Protean TGX gel system (Bio-Rad) and transferred to PVDF (0.45 μm pore size ...
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bioRxiv - Immunology 2019Quote: ... total proteins were quantified in paw extracts using the Bicinchoninic acid technique (BCA) and 75-100µg of proteins were loaded on 12% polyacrylamide gels (Biorad). The migration step was followed by a liquid transfer on PVDF membranes (100V 40min ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein-antibody complexes were collected by incubating protein extracts with 20 μl SureBeads Protein G magnetic beads (Bio-Rad) for 30 min at room temperature on a rocking shaker ...
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bioRxiv - Plant Biology 2021Quote: ... The concentration of the purified protein extracts was measured using the Bradford method (Protein Assay Dye Reagent, Bio-Rad). Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Biochemistry 2021Quote: ... 120-250 μg of crude extracts were run in a pre-cast 4-12% SDS-PAGE gradient gel (Biorad) and then electro-transferred onto a 0.2 μm nitrocellulose membrane (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: The total protein in each extract was quantified using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), and 24 μg run in a 10% SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2022Quote: ... total protein extracts were subjected to SDS page electrophoresis using 4-20% polyacrylamide gradient gels (Bio-Rad, Watford, UK) and transferred to nitrocellulose membranes using a Trans-blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations in cell-free extracts and purified protein preparations were quantified using Bradford reagent (catalog # 5000006, Bio-Rad), with ovalbumin as a standard ...
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bioRxiv - Immunology 2022Quote: ... 30 ug of Protein extracts were separated by 4-12% Bold NuPage (ThermoScientific) and transferred onto PVDF membranes (BioRad). After blocking with 3% albumin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal mass of each cell extract (20 μg) was separated on a 4-20% Mini-PROTEAN TGX gel (BioRad) and then transferred to Immobilon P membrane (Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was then centrifuged through a 0.45-micron filter at 4,000 x g and the resulting cell extract was measured using the DC-Lowry assay (BioRad). One milligram of total cellular protein was then incubated with 30 μl bed volume of anti-V5 beads (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were subsequently aliquoted by mixing 75μl of protein extract with 25μl of 4X Laemmli buffer (Bio-Rad #1610747) and stored at -20°C ...
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bioRxiv - Immunology 2021Quote: ... Protein extracts were separated by SDS-PAGE with a 10% gel and transferred to nitrocellulose membranes (Bio-Rad Laboratories). The membranes were probed with anti-spike Ab (40150-T62 ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µg of protein extract were loaded onto a Mini-PROTEAN TGX Precast Gel (4-15% gradient, BioRad, 4561086) in 1xLaemmli buffer (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... 20 µl of protein extract was mixed with 180 µl of five-time diluted Bradford reagent (Bio-Rad, US) in a 96-well microplate ...
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bioRxiv - Molecular Biology 2023Quote: ... and equal amounts of cell extracts were separated by SDS 12% PAGE and transferred to nitrocellulose membrane (Bio-Rad). Samples were then analyzed by Western blotting using a rabbit-raised anti-p53 polyclonal antibody (CM1 ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: Protein extracts were run on sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred to nitrocellulose membranes (Turbo blot, Biorad). Membranes were blocked for 2 hours in 5% non-fat milk (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Parasite extracts were resolved by SDS–PAGE gel electrophoresis then transferred to Transblot turbo mini-size nitrocellulose membranes (Biorad) and blocked for 1 h in 3% BSA in PBS at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... and equal amounts of cell extracts were separated by SDS 12% PAGE and transferred to nitrocellulose membrane (Bio-Rad). Samples were then analyzed by Western blotting using a rabbit-raised anti-p53 polyclonal antibody (CM1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were electrophoresed under reducing conditions on 4– 15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Cell Biology 2024Quote: Protein extracts dissolved in Lämmli sample buffer or SDS sample buffer were separated by SDS-PAGE (Biorad Mini Protean) and transferred to ethanol activated Amersham Hybond-P 0.45 µm PVDF membranes (Cytiva ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes received a final wash in PBST and the antigen-antibody complex was detected by enhanced chemiluminescence using a ChemiDoc MP Imaging system (Bio-Rad Laboratories, Hercules, CA). The bands in the experimental samples were background subtracted and quantified by mean optical density using computer-assisted densitometry with Image Lab software (v4.0.1 ...