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Citations for Bio-Rad :
251 - 300 of 459 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 30μg whole-cell extracts were separated by SDS–PAGE and transferred onto Nitrocellulose membrane (Bio-Rad). After blocking in 5% milk in TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the cell extracts was determined using the BCA assay kit (Bio-Rad). Protein concentration for lysates was adjusted to 2 μg/μl before adding 4X loading buffer (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of extracts were resuspended in reducing (5.0 % 2-mercaptoethanol) sample buffer (Bio-Rad) and boiled at 100°C for 5 min ...
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bioRxiv - Physiology 2023Quote: ... These extracts were loaded onto a 4-20 % SDS-PAGE gel (Bio-Rad, Hercules, California, USA), transferred onto nitrocellulose membrane and revealed with a rabbit anti-target antibody (see Table E2) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the extracts were determined using a Bio-Rad protein assay kit (Biorad, 5000006). Proteins were diluted in Invitrogen 2X sample buffer (NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the extracts were determined using a Bio-Rad protein assay kit (Biorad, 5000006). The same amounts of proteins were incubated with antibodies (anti-SURF2 or anti-GAPDH antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the extracts were determined using a Bio-Rad protein assay kit (Biorad, 5000006). The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of the extracts was measured using a commercial reagent (Bio-Rad Protein Assay). Acetyltransferase activity was assessed using the phosphocellulose paper binding assay [Haas & Dowding ...
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bioRxiv - Plant Biology 2024Quote: Total protein extract (10 µg) was separated by SDS-PAGE and transferred to PVDF membranes (BioRad). For detection of biotinylated proteins as result of TurboID activity ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μg of protein extracts were mixed with 4x Laemmli sample buffer with 2-mercaptoethanol (Biorad) and heated at 95°C for 10 minutes ...
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bioRxiv - Microbiology 2019Quote: ... The antigen-antibody reaction was analyzed by using Opti-4CN detection kit according to the instructions of the manufacturer (Biorad).
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bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and affinity purified anti-HSP-90 antibodies by use of an MBP-HSP-90 antigen coupled to Affigel matrix (BioRad), using a procedure previously published (Benian et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The BRM antigen was affinity-purified by IMAC using the NGC Medium-Pressure Liquid Chromatography System (Bio-Rad, Hercules, CA) and eluted in elution buffer (50mM sodium phosphate pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-12 (10 ng/mL)] or transferred to a high-binding 96-well plate pre-coated with indicated amounts of control or TAG72 antigen (BioRad). Reagents and buffers for flow cytometry processing were pre-chilled on ice unless otherwise stated ...
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bioRxiv - Epidemiology 2019Quote: ... Bloodspot dilutions were assayed in duplicate with antigen-coupled microsphere beads using a BioPlex 200 system platform (Bio-Rad, Hercules, CA) as previously described [21,23,25] ...
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bioRxiv - Biochemistry 2021Quote: ... The antigen-antibody complexes were visualized using appropriate HRP conjugated secondary antibodies and enhanced chemiluminescence kit (ECL; Amersham or Bio-Rad) and normalization was reported to β-actin ...
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bioRxiv - Immunology 2023Quote: ... Labeled glycans were dissolved in HPLC grade water (FisherScientific) and excess unbound APTS removed using Agencourt CleanSEQ beads (non-antigen specific glycans) and Bio-Gel P-2 (Bio-rad) size exclusion resin (RBD-specific glycans) ...
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bioRxiv - Neuroscience 2023Quote: ... The antigen–antibody complexes were detected with SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoScientific) using the ChemiDoc XRS+ Imager System (Bio-Rad). Blots were exposed for 3 seconds ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Molecular Biology 2023Quote: ... The HURP antibody was then purified by first conjugating 10 mg of HURP(462-576) antigen to 0.5 mL of Affi-gel 15 (Bio-Rad: 1536051) resin (brought up to 10 mL with PBS buffer) ...
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bioRxiv - Microbiology 2023Quote: ... Distinct MagPlex microsphere sets (Luminex Corp, Austin, TX, USA) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration of the supernatant (“total protein extract”) was determined by Bradford protein assay kit (Bio-Rad). An equal amount of total protein was loaded on 12% SDS acrylamide gels (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Protein extracts collected after centrifugation were subjected to SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). After blocking with PBS containing 0.1% Tween-20 and 5% milk ...
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bioRxiv - Developmental Biology 2019Quote: Nuclear extracts were fractionated by SDS-PAGE and transferred to nitrocellulose by semi-dry transfer (Bio-Rad). Membrane were blocked with 5% nonfat milk in PBS-0.1% Tween 20 for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were deposited onto a 4-15% Mini-PROTEAN® TGXTM precast protein gel (Bio-Rad) for migration ...
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bioRxiv - Cancer Biology 2020Quote: Cell extracts were harvested by direct addition of 2x SDS sample buffer (1610747, BioRad Laboratories, Alcobendas, Spain) containing 100 mM DTT to the culture plate and incubation on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: 30 μg protein extracts were separated on 7.5% gradient polyacrylamide gel (Mini-PROTEAN TGX gel, Bio-Rad) by electrophoresis (SDS-PAGE) ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Equal amounts of protein extract were loaded onto 4-15% Mini-PROTEAN TGX precast gels (Bio-Rad), separated by SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μl of the protein extract was used to carry out a Bradford (Bio-Rad, 500-0205) assay ...
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bioRxiv - Cancer Biology 2019Quote: ... Extracts were separated by SDS– PAGE and transferred to 0.2 µm pore size nitrocellulose membranes (Bio-Rad). Blots were probed with primary antibodies to caspase-3 (#9662) ...
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bioRxiv - Plant Biology 2020Quote: ... The remaining extract was used for total protein determination using the Bradford microplate microassay procedure (Bio-Rad).
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bioRxiv - Genomics 2021Quote: ... whole-cell extract in the supernatant was measured using the Bio-Rad Protein Assay (Bio-Rad, 5000006). 15 μg of protein extracts were mixed with NuPAGE LDS Sample Buffer (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... Total soluble protein content of the protein extracts was determined using Bradford protein assay (Bio-Rad, USA) with bovine serum albumin (BSA ...
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bioRxiv - Genomics 2022Quote: ... 20 µg total protein extract per sample were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cytoplasmic extract prepared from the harvested cells was used for immunoprecipitation (IP) using ProteinG magnetic beads (Biorad). Beads were first equilibrated with IP buffer (25 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2024Quote: ... 40 µg of protein extract was loaded on 10% polyacrylamide gels (TGX Stain-Free FastCast, Bio-Rad). Membranes were blocked with 5% BSA-TBST (5% BSA Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... The extracts were then desalted on Bio-Gel® P-6DG gel (BIO-RAD, Hercules, CA, USA) equilibrated with 20 mM sodium phosphate buffer pH 7.4 containing 0.15 M NaCl ...
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bioRxiv - Microbiology 2023Quote: ... 7 µg of each cell extract was loaded on a 12% stain-free SDS-PAGE gel (BioRad) and separated by gel electrophoresis at 175 V for 45 min ...
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bioRxiv - Microbiology 2020Quote: ... Distinct MagPlex microsphere sets (Luminex Corp) were respectively coupled to viral or control antigens using the amine coupling kit (Bio-Rad Laboratories) according to manufacturers’ instructions ...
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bioRxiv - Pathology 2019Quote: ... Affi-gel 10 affinity resins were covalently cross-linked to his-tagged GT198 protein as antigen for affinity purification of anti-GT198 according to the manufacturer’s protocol (Bio-Rad, Hercules, CA). FFPE tumor sections or tumor microarrays were deparaffinized and dehydrated through xylene and ethanol series ...
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bioRxiv - Molecular Biology 2021Quote: ... Initial screening for IgG antibodies was undertaken using an extensive panel of bacterial (Leptospira, Brucella) and viral antigens (paramyxovirus, filovirus, coronavirus, flavivirus, alphavirus) coupled to MagPlex beads (Bio-Rad, Australia) for multiplex screening ...
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bioRxiv - Immunology 2023Quote: ... Each antigen concentration was determined by UV absorbance at 450 nm in a plate reader (iMark™ Microplate Absorbance Reader, BioRad, Japan).
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed as previously mentioned and the antigen-antibody complex was revealed by Clarity Enhanced Chemiluminescence Blotting Substrate (Bio-Rad, #1705060) using the iBright™ FL100 Imaging System (ThermoFisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein extract equivalent to 50 μg was loaded per lane onto precast gradient gels (4-20% Bio-rad). Separated proteins were then transferred to nitrocellulose membranes ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... protein extracts were prepared in lysis buffer with 2% SDS and resolved in a 4-12% gel (Biorad). Membranes were probed overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 15 µg of protein extract were loaded with 5x Laemmli buffer on Mini Protean TGX gels (Bio-Rad) and run at 100V for 1.5 h ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of whole cell extracts and tissue lysates were determined using a Bradford Protein assay (Bio-Rad). 50μg of sample proteins per well were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2019Quote: ... 30 μL egg extract was applied to and eluted from a Micro Bio-Spin 6 column (Bio-Rad) equilibrated in ELB150 ...