Labshake search
Citations for Bio-Rad :
201 - 250 of 459 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 20-30 μg of extract was loaded into gradient SDS-PAGE gels (Bio-Rad) and transferred to nitrocellulose membranes (Amersham Proton 0.2 μM NC #10600006) ...
-
bioRxiv - Genetics 2023Quote: ... Pre-cleared extracts were resolved on 12% precast polyacrylamide gel (Bio-Rad, cat. 4561043) and blotted on a PVDF membrane (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... and the extract concentrations were measured using the Bradford method (Bio-Rad Bradford reagent). All sample extracts were adjusted to 1 mg/mL ...
-
bioRxiv - Microbiology 2024Quote: Parasite or digitonin fractionated extracts were resuspended in 2X Laemmli sample buffer (Bio-Rad) with 5% 2-mercaptoethanol (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: Proteins from whole cell extracts were resolved on 4-20% SDS-polyacrylamide gels (BioRad), followed by transfer of the proteins to 0.2 µm nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... whole-cell extracts were resolved using 4-20% Mini-PROTEAN TGX precast gels (BioRad) and transferred to PVDF membranes (Millipore) ...
-
bioRxiv - Bioengineering 2022Quote: ... and EV/HSP27 mixtures containing 2 µg of HSP27 at the indicated w/w ratios were mixed with native sample buffer (cat#1610738, Bio-Rad Laboratories Inc., Hercules, CA) and loaded into the gel lanes ...
-
bioRxiv - Pathology 2019Quote: ... Extracts with equal amounts of proteins were solubilized by SDS sample buffer (BioRad, Califonia, USA), separated by SDS-PAGE ...
-
bioRxiv - Biophysics 2019Quote: ... The extract containing l-Opa1 was loaded onto a Ni-NTA column (Biorad, CA, USA), washed with 40 column volumes of buffer B (50 mM sodium phosphate ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNAs were synthesized from total RNA extracts using iScript cDNA Synthesis Kit (Bio-Rad, US) and cDNA products stored at 4 °C before use ...
-
bioRxiv - Cell Biology 2021Quote: Proteins from whole cell extracts were resolved on 4-20% SDS-polyacrylamide gels (BioRad, #4561096), followed by transfer of the proteins to 0.2 µm nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Plant Biology 2022Quote: ... total protein content in the extracts was quantified with a Bradford assay (Bio-Rad; 5000006). The assay was performed using 50 μg of total protein extract and 300 μM of substrate in assay buffer (100mM HEPES-KOH pH 7.8 ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins extracts(ca.20μg) were resolved by SDS-PAGE (4 - 15 % Mini-PROTEAN TGX, Biorad) and transferred onto a nitrocellulose membrane and were blocked according to appropriate primary antibodies Membranes were visualized using an Odyssey® DLx scanner using Odyssey® imaging software ...
-
bioRxiv - Plant Biology 2019Quote: ... The protein concentration of the protein extract was then measured by Bradford (BioRad protein assay). The protein extract was diluted 1:5 to avoid interference of buffer components with the Bradford assay ...
-
bioRxiv - Molecular Biology 2019Quote: Total whole cell extracts from SCGs were collected by lysis in Laemmli buffer (Bio-Rad) from approximately 50,000 neurons and fractionated on NuPAGE 4-12% Bis-Tris Protein Gels (Life Technologies) ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein concentration in the soluble extract was determined using the DC Protein Assay (Bio-Rad), as per the manufacturer’s protocol.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells extracts were resolved on 12% SDS-PAGE and transferred to nitrocellulose membrane (Bio-Rad). Immunoblot analysis was performed by overnight incubation with antibodies against BiP ...
-
bioRxiv - Microbiology 2023Quote: ... Protein concentration in the extracts was quantified using Bradford protein assay (BioRad®: 500-0006). Depending on the experiment ...
-
bioRxiv - Bioengineering 2022Quote: ... Protein extracts were quantified by a Bradford protein assay (Bio-Rad Laboratories, Hercules, CA, USA). Fifteen micrograms total protein extracts were separated by 4–20 % stain-Free SDS-PAGE gels and transferred onto polyvinylidene difluoride (PVDF ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad) for immunoblotting ...
-
bioRxiv - Developmental Biology 2023Quote: ... Nuclear extracts were separated by SDS–PAGE and transferred onto a nitrocellulose membrane (Bio-Rad). Membranes were blocked with 3% bovine serum albumin (BSA ...
-
bioRxiv - Neuroscience 2023Quote: ... 30 µg of protein extract was resolved on 4-15% polyacrylamide gels (Bio-Rad, 4561085), transferred to PVDF membranes (Thermo Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... The extract was cleared by centrifugation and protein concentration was determined by Bradford assay (BioRad). Cell lysate extracted from different genotypes under the same starvation treatment at the same time point were then mixed at equal amounts with an external control sample (wild type cells in exponential growth phase in rich medium ...
-
bioRxiv - Immunology 2023Quote: ... solubilised cell extracts were mixed with reducing Laemmli SDS Sample Buffer 6x (Bio-Rad, USA) and boiled at 95°C for 5 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... The concentration of the protein extracts was measured using Protein Assay Dye Reagent (BioRad, #5000006).
-
bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were separated on NuPAGE 4–20% Bis-Tris gels (Bio-Rad, Hercules, CA) and blotted onto PVDF membranes (Bio-Rad ...
-
bioRxiv - Biophysics 2019Quote: ... All custom-made antibodies were purified from serum with an antigen-coupled matrix (Affi-Gel 10 or 15, Biorad). For western blot analysis of other proteins ...
-
bioRxiv - Biochemistry 2023Quote: ... The antibody–antigen reactions were detected using the Clarity™ or Clarity™ MaxWestern Blotting ECL Substrates (Bio-Rad). Densitometry analysis was performed using ImageJ software11–13 ...
-
bioRxiv - Microbiology 2022Quote: ... The lysates were adjusted to 5 µg of total soluble proteins in native sample buffer and then loaded onto a 4-20% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad, Cat. No. 4561094). Protein electrophoreses were performed in TG buffer (60 mM Tris ...
-
bioRxiv - Genetics 2021Quote: Protein extracts were prepared in Laemmli 2X buffer and western blots performed using SDS gels (BioRad) and Trans-Blot Turbo Transfer System ...
-
bioRxiv - Developmental Biology 2021Quote: ... Then denatured protein extract was separated in 4%-15% precast polyacrylamide gel (Bio-Rad, 456-1084) and then transferred to 0.45µm PVDF membrane ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein concentrations of the extracts was determined by the Bradford assay (Bradford reagent supplier: Bio-Rad) and the amount of total protein loaded from each sample was optimized to achieve similar GFP levels ...
-
bioRxiv - Molecular Biology 2019Quote: ... 10 µg of each extract was loaded on 7-12% precast SDS-polyacrylamide gels (Bio-Rad). After transference ...
-
bioRxiv - Cancer Biology 2019Quote: ... 50μg whole-cell extracts were separated by SDS–PAGE and transferred onto Nitrocellulose membrane (Bio-Rad). After blocking in 5% milk in TBST ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein extracts were fractionated on 4-20% Mini-PROTEAN® TGXTM Precast protein gels (Bio-Rad), transferred to a nitrocellulose blotting membrane (AmershamTM ProtranTM ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins in the extracts were resolved by SDS-PAGE using 12% or 4-15% gradient (BioRad) acrylamide gels ...
-
bioRxiv - Bioengineering 2021Quote: ... 15 µg of protein extracts were separated by Mini-PROTEAN TGXPrecast Gels (BioRad, Hercules, CA, USA) and transferred to PVDF membranes using Trans-Blot Turbo RTA Mini PVDF Transfer Kit (BioRad) ...
-
bioRxiv - Biophysics 2021Quote: ... The supernatant extract containing l-Opa1 was loaded onto a Ni-NTA column (Biorad, CA, USA), washed with 50 mM sodium phosphate ...
-
bioRxiv - Plant Biology 2021Quote: ... Each extract was assayed for protein levels with the Bio-Rad protein assay (Bio-Rad, USA). Enzyme activity (expressed in nkatal/mg protein ...
-
bioRxiv - Biochemistry 2021Quote: ... Nuclear protein extracts were obtained as described earlier(30) and quantified by Bradford reagent (Bio-rad) to determine the total amount of protein ...
-
bioRxiv - Physiology 2021Quote: ... Approximately 8μg of protein extract was loaded per lane on polyacrylamide gel (4-20% Criterion, BioRad). Proteins were separated and transferred to PVDF membrane ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were separated by electrophoresis on acrylamide gel (456-8085, Bio-Rad, Hercules, CA, USA) and transferred onto PVDF membrane ...
-
bioRxiv - Biophysics 2020Quote: ... 10μl of VLPs or cell extracts were then denatured by Laemmli sample buffer (BioRad, Hercules, CA) with 5% BME and boiled at 95C for 10min ...
-
bioRxiv - Developmental Biology 2022Quote: ... Whole cell extracts were fractionated by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad). After incubation with 5% milk in TBST (10 mM Tris ...
-
Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... cell extracts were quantified with the Bradford-based Protein Assay kit (Bio-Rad Protein Assay, #5000006). After addition of 6X Laemmli buffer (375 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2021Quote: Denatured protein extracts were separated by SDS-PAGE on 10% TGX Stain-Free™ (Bio-Rad) gels prepared according to the manufacturer’s instructions using a Stain-Free FastCast Acrylamide Kit ...
-
bioRxiv - Cell Biology 2019Quote: ... The protein extracts were subjected to electrophoresis through a 4–20% precast polyacrylamide gel (Bio-Rad). Subsequently ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cell extracts were sonicated 10 s and protein concentration determined using DCTM Protein Assay (Bio-Rad). Ten micrograms of proteins were loaded and run on NuPAGE 3-8% Tris-Acetate (ThermoFisher Scientific ...
-
bioRxiv - Bioengineering 2021Quote: ... protein extracts were loaded to fast protein liquid chromatography (FPLC, NGC Quest 10 Chromatography System, Biorad) for 3X PBS buffer exchange and purification ...
-
bioRxiv - Genetics 2022Quote: ... Protein extracts and supernatants were resolved by electrophoresis in Criterion TGX 10% precast gels (Bio-Rad), with the resulting bands transferred onto PVDF membranes (#1704157 ...