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Citations for Bio-Rad :
401 - 450 of 1535 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 2 μl of bacterial cells standardized to 104 cells/μl were added to 48 μl of master mix and vortexed to evenly disperse cells before adding 300 μl of cold Droplet Generation Oil for Probes (BioRad 1863005). Emulsions were formed immediately after by shaking tubes at 25 Hz for 30 seconds on a Retch Mixer Mill MM 400 with adapters 11990 and 11993 (Mobio/Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... The transcript level of different genes of interest was evaluated via qPCR machine using SYBR green master mix (MedChem Express, USA) and detected on CFX96TM Real-Time System C1000 Touch Thermal cycler (BIO-RAD); Conditions ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR experiments were carried out using SYBR Green FastStart Master Mix [25] on a CFX Connect Real-time PCR detection system (Bio-RAD). qPCR cycle settings were as follows ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR was conducted using primer sets for the indicated transcripts and iTaq Universal SYBR Green master mix (Bio-Rad).
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bioRxiv - Genomics 2022Quote: ... was used for RNA to cDNA conversion and subsequent qPCR analysis was performed using the SYBR Green Master Mix (Bio-Rad) and primers for retina-specific genes and controls ...
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bioRxiv - Genomics 2022Quote: ... as well as the shared duplicated region downstream of IRX1 were evaluated by qPCR using the SYBR Green Master Mix (Bio-Rad). Copy number values were analyzed in qbase+ (Biogazelle) ...
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bioRxiv - Cancer Biology 2022Quote: ... were performed using the FIREPol® Master Mix Ready to Load (Solis biodyne, 04-12-00115) and a T100 thermal cycler (Biorad). Sequences of the primers used are the following ones ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-03) in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) with 3 biological replicates ...
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bioRxiv - Plant Biology 2022Quote: ... and qPCR was performed using the SYBR Green Realtime PCR Master Mix (TOYOBO) on a CFX real-time machine (Bio-Rad). The PP2AA3 gene was used as the reference gene for normalization ...
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bioRxiv - Genetics 2022Quote: ... a master mix containing primers but not the template was made and aliquoted to 96-well PCR plates (Bio-Rad, HSP9601). Right before loading ...
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bioRxiv - Pathology 2023Quote: ... One nanogram of cDNA from each sample was mixed with forward and reverse primers (300 nM) and SYBR Green master mix (Bio-Rad). The amplification program was as follows ...
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bioRxiv - Genomics 2023Quote: ... RT-PCR was performed in triplicates and carried out in 20 µl of reaction mixture containing 10 µl of Fast SYBR® Green Master Mix (Biorad), 0.2 µl (10 pmol/µl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quantitative real-time PCR analysis was performed with ChamQ Universal SYBR qPCR Master Mix kit (Vazyme) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) under the conditions of 95 °C for 2 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The qRT-PCR assay was performed using ChamQ SYBR Master Mix (Vazyme, Q311-02) on Bio-rad CFX96 real-time PCR system (Biorad, USA). All primers used in the present study were listed as following ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qPCR was performed with gene-specific primers using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme #Q712) reaction system on the CFX96 Real-Time detection system (Bio-Rad). Each experiment was performed with three reaction replicates and UBQ10 was used as the internal control for data analysis ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then SYBR Green Real-Time PCR Master Mix (Yeasen, China) was used for qPCR on the CFX 96 system (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The qPCR was performed using TSINGKE Master qPCR Mix (Tsingke Biotechnology) and the MyiQ2 Real-time PCR Detection system (Bio-Rad) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... and qRT-PCR was performed using AceQ qPCR SYBR Green Master Mix (Vazyme) and corresponding primers on a CFX96 Touch Real-Time System (Bio-Rad). Primers used for amplifications are listed in Table 1 ...
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bioRxiv - Immunology 2023Quote: ... Equal volumes of cDNA samples were used in qRT-PCR reactions using Sso Advanced Universal SYBR Master Mix (Bio-Rad 1725271) and validated primers (41 ...
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bioRxiv - Immunology 2023Quote: ... The pre-amplified cDNA was subjected to qPCR in which the amplified product was detected using AceQ Universal SYBR qPCR Master Mix (Vazyme, Q511-03) on a CFX384 Real-Time PCR Detection System (Bio-rad).
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bioRxiv - Plant Biology 2023Quote: ... quantitative PCR was carried out using a ChamQ Universal SYBR qPCR Master Mix (VAZYME) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Actin2 (AT3G18780 ...
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bioRxiv - Microbiology 2023Quote: ... qPCR analysis was performed using ChamQ SYBR qPCR Master Mix (Q311, Vazyme) with a CFX ConnectTM Real-Time System (Bio-Rad). Bacillus subtilis (ATCC® 6051™ ...
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bioRxiv - Bioengineering 2023Quote: ... qRT-PCR was performed using SYBR Green Realtime PCR Master Mix (QPK-201, TOYOBO) on a CFX Connect Real-Time PCR Detection System (Bio-Rad). All samples were run in two technical replicates and normalized with respect to GAPDH levels using the 2-ΔΔCt method ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was run with cDNA previously generated from rescue experiments in a 10 µl reaction using iTaq Universal SYBR Green Master Mix (BioRad 1725121) with readout using the Applied Biosystems ViiA 7 real-time PCR system with standard cycling parameters (50°C for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... the FastStart Universal SYBR Green Master (ROX) mix was employed on a CFX96 Touch Real-Time PCR detection system (Bio-Rad). GAPDH was employed as the internal reference gene.
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bioRxiv - Immunology 2023Quote: ... The 5x-diluted RT samples for detection were mixed with ChamQ SYBR qPCR Master Mix (Q311-02, Vazyme, China) according to instruction and then run on CFX 96 (Biorad, USA). The expression level of target genes was normalized by the 2-ΔΔCt method after having checked that the efficiencies of the couple of primers were similar.
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bioRxiv - Neuroscience 2023Quote: ... Quantitative levels of mRNA were assayed in triplicates using the Universal SYBR Master Mix (MQ101-00, Vazyme) following the manufacturer’s instruction in the CFX Connect System (Bio-Rad Laboratories). Primers for DAT and ACTB are listed in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Cancer Biology 2023Quote: ... QPCR was performed using probe-based primer sets and master mix (Prime Time, Integrated DNA Technologies, Coralville, IA) on a CFX96 real time platform (Bio-Rad). Data was evaluated using Maestro software (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... followed by qPCR with PowerUp SYBR green PCR master mix on a CFX Connect Real-Time PCR Detection System (Bio-Rad). All reagents ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR assays were conducted using gene specific primers (Supplementary table X. Primer list) using SYBR green master mix (Bio-Rad). The reaction was run for a total of 40 cycles ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad) with Micro-Amp Optical 384-well plates ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was transferred to eppendorf and mixed with 2X loading dye (2X Lammeli Buffer, Bio-Rad, No. 1610737) + beta-Mercaptoethanol (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time qPCR analysis was carried out with the SYBR®Green Realtime PCR Master Mix (TOYOBO) on a CFX real-time machine (Bio-Rad). Two technical repeats were performed for each sample ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was run using the SYBR® Green Master Mix on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad). Gene expression was relative to the housekeeping gene Gapdh or Hprt and presented by 2-△Ct 52.
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bioRxiv - Immunology 2021Quote: Real-time PCR was constructed and read by the Bio-Rad CFX96 system using TaqMan PCR Master Mix (Bio-Rad, Cat# 1725284) and pre-mixed probe sets ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed on three biological replicates along with three technical replicates using the SYBR-green dye premixed master-mix (Thermo Fischer Scientific) in a C1000 Touch© thermal cycler (BIO-RAD). CT values were obtained using Bio-Rad CFX manager 3.1 ...
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bioRxiv - Biochemistry 2020Quote: ... PCR was performed using the AceQ qPCR SYBR Green Master Mix kit (Vazyme, Nanjing, China) and the MyiQ2 Real-time PCR system (Bio-Rad, USA) with the following cycling conditions ...
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bioRxiv - Neuroscience 2020Quote: ... levels were quantified by real time polymerase chain reaction (RT-PCR) using SYBR® Green DNA intercalating dye and master mix (Bio-Rad) and the ABI 7900HT PCR machine ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were performed with 2 × Taq Master Mix (Vazyme, Nanjing, China) on a Bio-Rad Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following procedure ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed on three biological replicates along with three technical replicates using the SYBR-green dye premixed master-mix (Thermo Fischer Scientific) in a C1000 Touch© thermal cycler (BIO-RAD). CT values were obtained using Bio-Rad CFX manager 3.1 ...
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bioRxiv - Microbiology 2022Quote: ... All the cDNA products were utilized for the quantitative real-time PCR reaction using the GoTaq qPCR Master Mix on a CFX96 real-time PCR Detection System (Bio-Rad, CA). Primer pairs were designed using Universal Probe library website and shown in Table S4 ...
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bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
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bioRxiv - Neuroscience 2021Quote: ... Lcn2 and C3 were quantified by quantitative real-time PCR (qRT-PCR) using the QuantStudio 7 Pro System thermo-cycler (ThermoFischer Scientific) and SYBR Green Master Mix (Bio-Rad Laboratories). Data are shown as fold change relative to control samples using the ΔΔCq method with Rpl4 as an internal control gene ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... and the corresponding 5 μl of Primer Master-mix was added followed by sealing the plate with Microseal ® adhesive sealer (Bio-Rad). The 60S acidic ribosomal protein P0 (rplp0) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Expression levels of ICAM-1 and IL6 mRNAs were quantified using SYBR Green-based primers and the iTaq master mix (Bio-Rad Inc) in the Bio-Rad CFX Real-Time PCR detection system (Bio-Rad Inc) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cDNA was with Taq Pro Universal SYBR qPCR Master Mix kit (Q712, Vazyme, Nanjing, China) by the CFX96TM Real-Time System (BIO-RAD, USA). The thermal cycles were 95 ℃ for 5 min ...
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bioRxiv - Systems Biology 2022Quote: ... paired gRNA entry vectors were created by PCR amplification (Red Taq DNA Polymerase Master Mix, VWR Life Science or iProof High-Fidelity DNA Polymerase, Bio-Rad Laboratories) on the template plasmid pEN-2xTaU3 with primers containing the 20-nt spacer sequences and BbsI restriction sites ...
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bioRxiv - Cancer Biology 2019Quote: ... The RT-PCR assay was performed using SYBR Green PCR Master Mix (QPK-212, Toyobo Co.) on the CFX96 real-time PCR detection system (Bio-Rad, USA). The gene-specific primer sequences are listed in Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCR reactions were performed as described by the manufacturer in triplicate with Power SYBR Green PCR Master Mix on the BIO-RAD CFX98 Real-Time System (BIO-RAD, US). The reaction was performed in a 96-well plate ...